LOCUS       sequence-234948-        9097 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Open reading frame vector encoding hcRed1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9097)
  AUTHORS   Hong AL, Guerriero JL, Doshi MB, Kynnap BD, Kim WJ, Schinzel AC, 
            Modiste R, Schlauch AJ, Adam RM, Kwiatkowski DJ, Beroukhim R, Letai 
            A, Rosenberg JE, Hahn WC
  TITLE     MCL1 and DEDD promote urothelial carcinoma progression.
  JOURNAL   Mol Cancer Res. 2019 Feb 18. pii: 1541-7786.MCR-18-0963. doi: 
            10.1158/1541-7786.MCR-18-0963.
  PUBMED    30777879
REFERENCE   2  (bases 1 to 9097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cancer 
            Res. 2019 Feb 18. pii: 1541-7786.MCR-18-0963. doi: 
            10.1158/1541-7786.MCR-18-0963."
COMMENT     Sequence Label: pLX307 hcRed1
FEATURES             Location/Qualifiers
     source          1..9097
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             40..642
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(40..59)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     536..556
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    685..802
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        940..2118
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1171..2109
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2066..2086
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    2138..2162
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2165..2848
                     /codon_start=1
                     /product="far-red fluorescent variant of Heteractis crispa 
                     chromoprotein"
                     /label=HcRed1
                     /note="mammalian codon-optimized"
                     /translation="MVSGLLKESMRIKMYMEGTVNGHYFKCEGEGDGNPFAGTQSMRIH
                     VTEGAPLPFAFDILAPCCEYGSRTFVHHTAEIPDFFKQSFPEGFTWERTTTYEDGGILT
                     AHQDTSLEGNCLIYKVKVHGTNFPADGPVMKNKSGGWEPSTEVVYPENGVLCGRNVMAL
                     KVGDRHLICHHYTSYRSKKAVRALTMPGFHFTDIRLQMLRKKKDEYFELYEASVARYSD
                     LPEKAN"
     protein_bind    complement(2850..2874)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2882..2923
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2974..3562
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3027..3047)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3445..3456)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3634..3867
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3945..4066
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3982..4001)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4036..4055
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4106..4241
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4261..4280)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4262..4280)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4290..4307)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4290..4306)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4299..4321)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4448..4903
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4535..4554)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4745..4766
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4929..5033
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5034..5102,5103..5894)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5252..5271)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6065..6653
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6554..6573
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6807..6824
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6941..6962
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6977..6982,6983..7000,7001..7007)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7015..7031
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7020..7042
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7039..7055
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7039..7055
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7074..7094
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7076..7094
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7122..7348
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7349..7529
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7576..7701
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8194..8427
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8612..8656
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8887..9097
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        join(8909..9097,1..8)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8956..9091
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
       61 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
      121 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
      181 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
      241 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
      301 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
      361 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
      421 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
      481 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
      541 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
      601 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
      661 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
      721 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      781 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
      841 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
      901 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
      961 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     1021 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     1081 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     1141 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     1201 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     1261 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     1321 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     1381 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     1441 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     1501 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     1561 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     1621 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     1681 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     1741 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     1801 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     1861 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     1921 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     1981 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     2041 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     2101 ccatttcagg tgtcgtgagg ctagcatcga ttgatcaaca agtttgtaca aaaaagttgg
     2161 caccatggtg agcggcctgc tgaaggagag tatgcgcatc aagatgtaca tggagggcac
     2221 cgtgaacggc cactacttca agtgcgaggg cgagggcgac ggcaacccct tcgccggcac
     2281 ccagagcatg agaatccacg tgaccgaggg cgcccccctg cccttcgcct tcgacatcct
     2341 ggccccctgc tgcgagtacg gcagcaggac cttcgtgcac cacaccgccg agatccccga
     2401 cttcttcaag cagagcttcc ccgagggctt cacctgggag agaaccacca cctacgagga
     2461 cggcggcatc ctgaccgccc accaggacac cagcctggag ggcaactgcc tgatctacaa
     2521 ggtgaaggtg cacggcacca acttccccgc cgacggcccc gtgatgaaga acaagagcgg
     2581 cggctgggag cccagcaccg aggtggtgta ccccgagaac ggcgtgctgt gcggccggaa
     2641 cgtgatggcc ctgaaggtgg gcgaccggca cctgatctgc caccactaca ccagctaccg
     2701 gagcaagaag gccgtgcgcg ccctgaccat gcccggcttc cacttcaccg acatccggct
     2761 ccagatgctg cggaagaaga aggacgagta cttcgagctg tacgaggcca gcgtggcccg
     2821 gtacagcgac ctgcccgaga aggccaactt gccaactttc ttgtacaaag tggttgatat
     2881 cggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat gaactagtac
     2941 cggttaagtc gacaatcaac gcgttaagtc gacaatcaac ctctggatta caaaatttgt
     3001 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     3061 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     3121 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     3181 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     3241 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     3301 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     3361 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     3421 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     3481 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     3541 tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag
     3601 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     3661 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     3721 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     3781 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     3841 tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc
     3901 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     3961 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     4021 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     4081 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     4141 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     4201 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     4261 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     4321 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     4381 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     4441 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     4501 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     4561 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     4621 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     4681 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     4741 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     4801 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     4861 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     4921 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     4981 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     5041 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     5101 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     5161 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     5221 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     5281 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     5341 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     5401 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     5461 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     5521 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     5581 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     5641 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     5701 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     5761 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     5821 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     5881 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     5941 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     6001 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     6061 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     6121 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     6181 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     6241 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     6301 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     6361 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     6421 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     6481 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     6541 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     6601 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     6661 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     6721 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     6781 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     6841 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     6901 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     6961 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     7021 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
     7081 accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact
     7141 cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa
     7201 gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca
     7261 acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt
     7321 atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg
     7381 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     7441 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     7501 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     7561 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     7621 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     7681 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     7741 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     7801 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     7861 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     7921 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     7981 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     8041 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     8101 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     8161 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     8221 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     8281 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     8341 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     8401 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     8461 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     8521 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     8581 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     8641 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     8701 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     8761 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     8821 gaggggaccc atgcattgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     8881 tccccagcag gcagaagtat gcaaagcatg cgtctcaatt agtcagcaac catagtcccg
     8941 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     9001 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     9061 cagaagtagt gaggaggctt ttttggaggc ctaggct
//
