LOCUS       sequence-234950-       11530 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Open reading frame vector encoding ARHGAP30.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11530)
  AUTHORS   Hong AL, Guerriero JL, Doshi MB, Kynnap BD, Kim WJ, Schinzel AC, 
            Modiste R, Schlauch AJ, Adam RM, Kwiatkowski DJ, Beroukhim R, Letai 
            A, Rosenberg JE, Hahn WC
  TITLE     MCL1 and DEDD promote urothelial carcinoma progression.
  JOURNAL   Mol Cancer Res. 2019 Feb 18. pii: 1541-7786.MCR-18-0963. doi: 
            10.1158/1541-7786.MCR-18-0963.
  PUBMED    30777879
REFERENCE   2  (bases 1 to 11530)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cancer 
            Res. 2019 Feb 18. pii: 1541-7786.MCR-18-0963. doi: 
            10.1158/1541-7786.MCR-18-0963."
COMMENT     Sequence Label: pLX311 ARHGAP30
FEATURES             Location/Qualifiers
     source          1..11530
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..211
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        23..219
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      70..205
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             241..636
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    710..827
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        965..2143
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1196..2134
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2091..2111
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    2163..2187
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4261..4272
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(5337..5348)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(5494..5518)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5526..5567
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5618..6206
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5671..5691)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6089..6100)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6278..6511
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6589..6710
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6626..6645)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6680..6699
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6750..6885
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6905..6924)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6906..6924)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6934..6951)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6934..6950)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6943..6965)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7092..7547
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7179..7198)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7389..7410
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7573..7677
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7678..7746,7747..8538)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7896..7915)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8709..9297
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9198..9217
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9451..9468
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9585..9606
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9621..9626,9627..9644,9645..9651)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9659..9675
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9664..9686
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9683..9699
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9683..9699
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9718..9738
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9720..9738
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9766..9992
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9993..10173
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10220..10345
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10838..11071
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11256..11300
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gcaggcagaa gtatgcaaag catgcgtctc aattagtcag caaccatagt cccgccccta
       61 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
      121 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
      181 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctttctag aggtaccacc
      241 atggccaagc ctttgtctca agaagaatcc accctcattg aaagagcaac ggctacaatc
      301 aacagcatcc ccatctctga agactacagc gtcgccagcg cagctctctc tagcgacggc
      361 cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc agaactcgtg
      421 gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga
      481 aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct tctcgatctg
      541 catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc agttgggatt
      601 cgtgaattgc tgccctctgg ttatgtgtgg gagggcctgc agctgcagta gtaaggcgcg
      661 ccgttaacga attctagatc ttgagacaaa tggcagtatt catccacaat tttaaaagaa
      721 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
      781 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
      841 gggacagcag agatccactt tggcgccggc tcgaggcctg caggtgcaaa gatggataaa
      901 gttttaaaca gagaggaatc tttgcagcta atggaccttc taggtcttga aaggagtggg
      961 aattggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     1021 ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga
     1081 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     1141 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag
     1201 tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg
     1261 aattacttcc acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg
     1321 gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc
     1381 ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc
     1441 tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt
     1501 ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg
     1561 ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct
     1621 gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt
     1681 gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc
     1741 accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg
     1801 gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt
     1861 tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct
     1921 cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc
     1981 gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat
     2041 gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca
     2101 gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgaggctagc atcgattgat
     2161 caacaagttt gtacaaaaaa gttggcacca tgaagtctcg gcagaaagga aagaagaagg
     2221 gcagcgcaaa ggagcgggtt tttgggtgcg acttgcagga gcacctgcag cactcaggcc
     2281 aggaggtgcc ccaggtgcta aagagctgtg cagaatttgt ggaggagtat ggagtggtgg
     2341 atgggatcta ccgcctctca ggggtctcct ccaacatcca gaagcttcgg caggaatttg
     2401 agtcagagcg gaagccagac ctgcgtcggg atgtttacct ccaagacatt cactgcgtct
     2461 cctccctgtg caaggcctat ttcagagaac tgccggatcc cctgctcact taccggctct
     2521 atgacaagtt tgctgaggct gtaggagtgc aattggaacc tgagcgcttg gtcaagatcc
     2581 tagaggtgct tcgggaactc cctgtcccaa actacaggac cctggagttc ctcatgaggc
     2641 acttggtaca catggcctca ttcagtgccc agaccaacat gcatgctcgc aacctggcca
     2701 tcgtgtgggc tcccaacctg ctgaggtcta aggacataga ggcctcaggc ttcaatggga
     2761 cagcggcctt catggaggtg cgggtacaat ccatcgtcgt ggagttcatc ctcacacacg
     2821 tggaccagct ctttgggggt gctgccctct ctggtggtga ggtggagagt gggtggcgat
     2881 cgcttccagg gacccgggca tcaggcagcc ccgaggacct tatgcccagg ccactgcctt
     2941 atcacctgcc tagcatactg caggctggcg atggaccccc acagatgcgg ccctaccata
     3001 ctatcatcga gattgcagag cacaagagga aggggtcttt gaaggtcagg aagtggaggt
     3061 ctatcttcaa tttaggtcgc tctggccatg agactaagcg taaacttcca cggggggctg
     3121 aggacaggga ggataaatcc aacaagggga cactgcggcc agccaaaagc atggactcac
     3181 tgagtgctgc agctggggcc agtgatgagc cagaggggct ggtggggccc agcagccccc
     3241 ggccaagccc attgctgcct gagagcttgg agaacgattc tatagaggca gcagagggtg
     3301 aacaggagcc tgaggcagaa gcactgggtg gcacaaactc tgaaccaggc acaccacgag
     3361 ctgggcggtc agccatccgg gctgggggca gcagccgtgc agaacgctgt gctggtgtcc
     3421 acatctcaga cccctacaat gtcaacctcc cgctacacat cacctctatc ctcagtgtgc
     3481 ccccgaacat catctctaac gtttccttgg ccaggctcac ccgtggcctt gagtgccctg
     3541 ctctacagca ccggccaagc cctgcctctg gccctggccc tggccctggc cttggccctg
     3601 gccccccaga tgaaaagttg gaagcaagtc cagcctcaag tcccctggca gactcaggcc
     3661 cagacgactt ggctcctgcc ctggaggact cgctgtccca ggaggtgcag gactccttct
     3721 ccttcctaga ggactcaagc agctcagaac ctgagtgggt gggggcagag gatggggagg
     3781 tggcccaggc agaagcagca ggagcagcct tctcccctgg ggaggacgac cctgggatgg
     3841 gctacctgga ggagctcctg ggagttgggc ctcaggtgga ggagttctct gtggagccac
     3901 ccctggatga cctgtctctg gatgaggcac agtttgtctt ggcccccagc tgctgttccc
     3961 tggactccgc tggccccagg cctgaagttg aggaggaaaa tggggaggaa gttttcctga
     4021 gtgcctatga tgacctaagt ccccttctgg gacctaaacc cccaatctgg aagggttcag
     4081 ggagtctgga gggagaggca gcaggatgtg gaaggcaggc tctgggacag ggtggggaag
     4141 agcaggcatg ctgggaagtt ggggaggaca agcaggctga gcctggaggc aggctagaca
     4201 tcagggaaga ggcagaggga agtccagaga ccaaggtgga ggctggaaag gccagtgagg
     4261 atagagggga ggctggggga agccaagaga caaaagtcag attgagagaa gggagtaggg
     4321 aagagacaga ggccaaggaa gagaagtcca aaggtcagaa gaaggctgac agtatggagg
     4381 ctaaaggtgt ggaggaacca ggaggagatg agtatacaga tgagaaggaa aaagaaattg
     4441 agagagaaga ggatgaacaa agagaggaag cccaggtaga agctggaagg gacctagagc
     4501 aaggggccca ggaagatcaa gttgctgagg agaaatggga agttgtacag aaacaagagg
     4561 ctgagggagt cagagaggat gaggacaaag gacagaggga gaaggggtac catgaagcaa
     4621 gaaaagacca aggagatggt gaagacagca gaagcccaga agcagcaact gaaggaggag
     4681 caggggaggt cagcaaggaa cgggagagtg gggatggaga ggctgaggga gaccagaggg
     4741 ctggagggta ctatttagaa gaggacaccc tctctgaagg ttcaggtgta gcgtccctgg
     4801 aggttgactg tgccaaagag ggcaatcctc actcttctga gatggaagag gtagccccac
     4861 agccacctca gccagaggag atggagcctg aggggcagcc cagtccagac ggctgtctat
     4921 gcccctgttc tcttggcctg ggtggcgtgg gcatgcgtct agcttccact ctggttcagg
     4981 tccaacaggt ccgctctgtg cctgtggtgc cccccaagcc acagtttgcc aagatgccca
     5041 gtgcaatgtg tagcaagatt catgtggcac ctgcaaatcc atgcccgagg cctggccggc
     5101 ttgatgggac tcctggagaa agggcttggg ggtcccgagc ttctcgatcc tcttggagga
     5161 atgggggtag tctttccttt gatgctgctg tggccctagc ccgggaccgc caaaggactg
     5221 aggctcaagg agttcggcga acccagacct gtactgaggg tggggattac tgcctcatcc
     5281 ccagaacctc cccttgtagc atgatctctg cccattctcc tcggcccctt agctgcctgg
     5341 agctcccatc tgaaggtgca gaagggtctg gatcccggag tcgtcttagt ctgcccccca
     5401 gagaacccca ggttcctgac cccctgttgt cctctcagcg caggtcatat gcatttgaaa
     5461 cacaggctaa ccctgggaaa ggtgaaggac tgtgcccaac tttcttgtac aaagtggttg
     5521 atatcggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgaacta
     5581 gtaccggtta agtcgacaat caacgcgtta agtcgacaat caacctctgg attacaaaat
     5641 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     5701 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     5761 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     5821 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     5881 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     5941 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     6001 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     6061 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     6121 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     6181 gatctccctt tgggccgcct ccccgcgtcg actttaagac caatgactta caaggcagct
     6241 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     6301 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     6361 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     6421 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     6481 cccttttagt cagtgtggaa aatctctagc agtacgtata gtagttcatg tcatcttatt
     6541 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     6601 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     6661 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     6721 ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     6781 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     6841 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa
     6901 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga
     6961 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     7021 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     7081 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     7141 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     7201 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     7261 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     7321 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     7381 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     7441 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     7501 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
     7561 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     7621 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     7681 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     7741 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     7801 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     7861 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     7921 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     7981 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     8041 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     8101 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     8161 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     8221 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     8281 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     8341 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     8401 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     8461 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     8521 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     8581 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     8641 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     8701 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     8761 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     8821 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     8881 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     8941 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     9001 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     9061 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     9121 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     9181 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     9241 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     9301 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     9361 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     9421 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     9481 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     9541 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     9601 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     9661 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca
     9721 attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa
     9781 tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga
     9841 aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa
     9901 ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata
     9961 ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag
    10021 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
    10081 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
    10141 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
    10201 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
    10261 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
    10321 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
    10381 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
    10441 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
    10501 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
    10561 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
    10621 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
    10681 agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
    10741 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
    10801 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
    10861 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
    10921 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
    10981 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
    11041 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
    11101 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
    11161 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
    11221 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
    11281 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
    11341 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
    11401 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
    11461 ccccgagggg acccatgcat tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
    11521 aggctcccca
//
