LOCUS       sequence-234952-       11716 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Open reading frame vector encoding ARHGAP30.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11716)
  AUTHORS   Hong AL, Guerriero JL, Doshi MB, Kynnap BD, Kim WJ, Schinzel AC, 
            Modiste R, Schlauch AJ, Adam RM, Kwiatkowski DJ, Beroukhim R, Letai 
            A, Rosenberg JE, Hahn WC
  TITLE     MCL1 and DEDD promote urothelial carcinoma progression.
  JOURNAL   Mol Cancer Res. 2019 Feb 18. pii: 1541-7786.MCR-18-0963. doi: 
            10.1158/1541-7786.MCR-18-0963.
  PUBMED    30777879
REFERENCE   2  (bases 1 to 11716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cancer 
            Res. 2019 Feb 18. pii: 1541-7786.MCR-18-0963. doi: 
            10.1158/1541-7786.MCR-18-0963."
COMMENT     Sequence Label: pLX307 ARHGAP30
FEATURES             Location/Qualifiers
     source          1..11716
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             147..191
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        422..632
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        444..640
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      491..626
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             672..1274
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(672..691)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1168..1188
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    1317..1434
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1572..2750
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1803..2741
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2698..2718
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    2770..2794
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4868..4879
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(5944..5955)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(6101..6125)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6133..6174
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6225..6813
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6278..6298)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6696..6707)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6885..7118
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7196..7317
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7233..7252)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7287..7306
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7357..7492
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7512..7531)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7513..7531)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7541..7558)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7541..7557)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7550..7572)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7699..8154
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7786..7805)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7996..8017
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8180..8284
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8285..8353,8354..9145)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8503..8522)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9316..9904
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9805..9824
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10058..10075
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10192..10213
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10228..10233,10234..10251,10252..10258)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10266..10282
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10271..10293
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10290..10306
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10290..10306
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10325..10345
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10327..10345
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10373..10599
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10600..10780
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10827..10952
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11445..11678
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
       61 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
      121 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
      181 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
      241 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
      301 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
      361 gacccatgca ttgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
      421 agcaggcaga agtatgcaaa gcatgcgtct caattagtca gcaaccatag tcccgcccct
      481 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
      541 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
      601 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctttcta gaggtaccac
      661 cggagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
      721 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
      781 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
      841 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgccgtg gcggtctgga
      901 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
      961 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     1021 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     1081 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     1141 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     1201 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     1261 ccggtgccag ttaacgaatt ctagatcttg agacaaatgg cagtattcat ccacaatttt
     1321 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     1381 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
     1441 tattacaggg acagcagaga tccactttgg cgccggctcg aggcctgcag gtgcaaagat
     1501 ggataaagtt ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag
     1561 gagtgggaat tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
     1621 agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa
     1681 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt
     1741 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
     1801 aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg
     1861 tgccttgaat tacttccacc tggctgcagt acgtgattct tgatcccgag cttcgggttg
     1921 gaagtgggtg ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag
     1981 ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct
     2041 gtctcgctgc tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc
     2101 tttttttctg gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg
     2161 tttttggggc cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc
     2221 ggggcctgcg agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg
     2281 ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc
     2341 ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct
     2401 caaaatggag gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa
     2461 gggcctttcc gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca
     2521 ggcacctcga ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt
     2581 tttatgcgat ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc
     2641 acttgatgta attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca
     2701 agcctcagac agtggttcaa agtttttttc ttccatttca ggtgtcgtga ggctagcatc
     2761 gattgatcaa caagtttgta caaaaaagtt ggcaccatga agtctcggca gaaaggaaag
     2821 aagaagggca gcgcaaagga gcgggttttt gggtgcgact tgcaggagca cctgcagcac
     2881 tcaggccagg aggtgcccca ggtgctaaag agctgtgcag aatttgtgga ggagtatgga
     2941 gtggtggatg ggatctaccg cctctcaggg gtctcctcca acatccagaa gcttcggcag
     3001 gaatttgagt cagagcggaa gccagacctg cgtcgggatg tttacctcca agacattcac
     3061 tgcgtctcct ccctgtgcaa ggcctatttc agagaactgc cggatcccct gctcacttac
     3121 cggctctatg acaagtttgc tgaggctgta ggagtgcaat tggaacctga gcgcttggtc
     3181 aagatcctag aggtgcttcg ggaactccct gtcccaaact acaggaccct ggagttcctc
     3241 atgaggcact tggtacacat ggcctcattc agtgcccaga ccaacatgca tgctcgcaac
     3301 ctggccatcg tgtgggctcc caacctgctg aggtctaagg acatagaggc ctcaggcttc
     3361 aatgggacag cggccttcat ggaggtgcgg gtacaatcca tcgtcgtgga gttcatcctc
     3421 acacacgtgg accagctctt tgggggtgct gccctctctg gtggtgaggt ggagagtggg
     3481 tggcgatcgc ttccagggac ccgggcatca ggcagccccg aggaccttat gcccaggcca
     3541 ctgccttatc acctgcctag catactgcag gctggcgatg gacccccaca gatgcggccc
     3601 taccatacta tcatcgagat tgcagagcac aagaggaagg ggtctttgaa ggtcaggaag
     3661 tggaggtcta tcttcaattt aggtcgctct ggccatgaga ctaagcgtaa acttccacgg
     3721 ggggctgagg acagggagga taaatccaac aaggggacac tgcggccagc caaaagcatg
     3781 gactcactga gtgctgcagc tggggccagt gatgagccag aggggctggt ggggcccagc
     3841 agcccccggc caagcccatt gctgcctgag agcttggaga acgattctat agaggcagca
     3901 gagggtgaac aggagcctga ggcagaagca ctgggtggca caaactctga accaggcaca
     3961 ccacgagctg ggcggtcagc catccgggct gggggcagca gccgtgcaga acgctgtgct
     4021 ggtgtccaca tctcagaccc ctacaatgtc aacctcccgc tacacatcac ctctatcctc
     4081 agtgtgcccc cgaacatcat ctctaacgtt tccttggcca ggctcacccg tggccttgag
     4141 tgccctgctc tacagcaccg gccaagccct gcctctggcc ctggccctgg ccctggcctt
     4201 ggccctggcc ccccagatga aaagttggaa gcaagtccag cctcaagtcc cctggcagac
     4261 tcaggcccag acgacttggc tcctgccctg gaggactcgc tgtcccagga ggtgcaggac
     4321 tccttctcct tcctagagga ctcaagcagc tcagaacctg agtgggtggg ggcagaggat
     4381 ggggaggtgg cccaggcaga agcagcagga gcagccttct cccctgggga ggacgaccct
     4441 gggatgggct acctggagga gctcctggga gttgggcctc aggtggagga gttctctgtg
     4501 gagccacccc tggatgacct gtctctggat gaggcacagt ttgtcttggc ccccagctgc
     4561 tgttccctgg actccgctgg ccccaggcct gaagttgagg aggaaaatgg ggaggaagtt
     4621 ttcctgagtg cctatgatga cctaagtccc cttctgggac ctaaaccccc aatctggaag
     4681 ggttcaggga gtctggaggg agaggcagca ggatgtggaa ggcaggctct gggacagggt
     4741 ggggaagagc aggcatgctg ggaagttggg gaggacaagc aggctgagcc tggaggcagg
     4801 ctagacatca gggaagaggc agagggaagt ccagagacca aggtggaggc tggaaaggcc
     4861 agtgaggata gaggggaggc tgggggaagc caagagacaa aagtcagatt gagagaaggg
     4921 agtagggaag agacagaggc caaggaagag aagtccaaag gtcagaagaa ggctgacagt
     4981 atggaggcta aaggtgtgga ggaaccagga ggagatgagt atacagatga gaaggaaaaa
     5041 gaaattgaga gagaagagga tgaacaaaga gaggaagccc aggtagaagc tggaagggac
     5101 ctagagcaag gggcccagga agatcaagtt gctgaggaga aatgggaagt tgtacagaaa
     5161 caagaggctg agggagtcag agaggatgag gacaaaggac agagggagaa ggggtaccat
     5221 gaagcaagaa aagaccaagg agatggtgaa gacagcagaa gcccagaagc agcaactgaa
     5281 ggaggagcag gggaggtcag caaggaacgg gagagtgggg atggagaggc tgagggagac
     5341 cagagggctg gagggtacta tttagaagag gacaccctct ctgaaggttc aggtgtagcg
     5401 tccctggagg ttgactgtgc caaagagggc aatcctcact cttctgagat ggaagaggta
     5461 gccccacagc cacctcagcc agaggagatg gagcctgagg ggcagcccag tccagacggc
     5521 tgtctatgcc cctgttctct tggcctgggt ggcgtgggca tgcgtctagc ttccactctg
     5581 gttcaggtcc aacaggtccg ctctgtgcct gtggtgcccc ccaagccaca gtttgccaag
     5641 atgcccagtg caatgtgtag caagattcat gtggcacctg caaatccatg cccgaggcct
     5701 ggccggcttg atgggactcc tggagaaagg gcttgggggt cccgagcttc tcgatcctct
     5761 tggaggaatg ggggtagtct ttcctttgat gctgctgtgg ccctagcccg ggaccgccaa
     5821 aggactgagg ctcaaggagt tcggcgaacc cagacctgta ctgagggtgg ggattactgc
     5881 ctcatcccca gaacctcccc ttgtagcatg atctctgccc attctcctcg gccccttagc
     5941 tgcctggagc tcccatctga aggtgcagaa gggtctggat cccggagtcg tcttagtctg
     6001 ccccccagag aaccccaggt tcctgacccc ctgttgtcct ctcagcgcag gtcatatgca
     6061 tttgaaacac aggctaaccc tgggaaaggt gaaggactgt gcccaacttt cttgtacaaa
     6121 gtggttgata tcggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa
     6181 tgaactagta ccggttaagt cgacaatcaa cgcgttaagt cgacaatcaa cctctggatt
     6241 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     6301 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     6361 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     6421 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     6481 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     6541 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     6601 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     6661 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     6721 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     6781 cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa
     6841 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     6901 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
     6961 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     7021 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     7081 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt acgtatagta gttcatgtca
     7141 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     7201 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     7261 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     7321 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     7381 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     7441 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg
     7501 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
     7561 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     7621 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     7681 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     7741 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     7801 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     7861 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     7921 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     7981 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     8041 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     8101 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg
     8161 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     8221 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     8281 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     8341 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     8401 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     8461 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     8521 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     8581 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     8641 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     8701 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     8761 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     8821 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     8881 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     8941 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     9001 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     9061 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     9121 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     9181 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     9241 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     9301 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     9361 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     9421 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     9481 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     9541 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     9601 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     9661 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     9721 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     9781 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     9841 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     9901 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     9961 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
    10021 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
    10081 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
    10141 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
    10201 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
    10261 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
    10321 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct
    10381 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa
    10441 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta
    10501 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc
    10561 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag
    10621 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
    10681 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
    10741 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
    10801 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
    10861 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
    10921 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
    10981 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
    11041 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
    11101 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
    11161 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
    11221 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
    11281 cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
    11341 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
    11401 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
    11461 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
    11521 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
    11581 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
    11641 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
    11701 aaactcattt gcacca
//
