LOCUS       sequence-234954-       11617 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Open reading frame vector encoding LacZ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11617)
  AUTHORS   Hong AL, Guerriero JL, Doshi MB, Kynnap BD, Kim WJ, Schinzel AC, 
            Modiste R, Schlauch AJ, Adam RM, Kwiatkowski DJ, Beroukhim R, Letai 
            A, Rosenberg JE, Hahn WC
  TITLE     MCL1 and DEDD promote urothelial carcinoma progression.
  JOURNAL   Mol Cancer Res. 2019 Feb 18. pii: 1541-7786.MCR-18-0963. doi: 
            10.1158/1541-7786.MCR-18-0963.
  PUBMED    30777879
REFERENCE   2  (bases 1 to 11617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cancer 
            Res. 2019 Feb 18. pii: 1541-7786.MCR-18-0963. doi: 
            10.1158/1541-7786.MCR-18-0963."
COMMENT     Sequence Label: pLX307 LacZ
FEATURES             Location/Qualifiers
     source          1..11617
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     380..400
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    529..646
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        784..1962
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1015..1953
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1910..1930
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1982..2006
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2021..5065
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAELQLSAGRYHYQLVWCQK"
     protein_bind    complement(5085..5109)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5162..5203
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    complement(5214..5238)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5246..5287
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5338..5926
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5391..5411)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5809..5820)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5998..6231
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6309..6430
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6346..6365)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6400..6419
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6470..6605
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6625..6644)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6626..6644)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6654..6671)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6654..6670)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      6812..7267
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6899..6918)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7109..7130
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7293..7397
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7398..7466,7467..8258)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7616..7635)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8429..9017
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8918..8937
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9171..9188
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9305..9326
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9341..9346,9347..9364,9365..9371)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9379..9395
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9384..9406
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9403..9419
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9403..9419
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9438..9458
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9440..9458
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9486..9712
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9713..9893
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9940..10065
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10558..10791
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10976..11020
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        11251..11461
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        11273..11469
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      11320..11455
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(11501..11617,1..486)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(11501..11520)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
       61 gacatcggca aggtgtgggt cgcggacgac ggcgccgccg tggcggtctg gaccacgccg
      121 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
      181 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
      241 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
      301 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
      361 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
      421 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
      481 agttaacgaa ttctagatct tgagacaaat ggcagtattc atccacaatt ttaaaagaaa
      541 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
      601 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
      661 ggacagcaga gatccacttt ggcgccggct cgaggcctgc aggtgcaaag atggataaag
      721 ttttaaacag agaggaatct ttgcagctaa tggaccttct aggtcttgaa aggagtggga
      781 attggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
      841 gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa
      901 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
      961 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt
     1021 gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga
     1081 attacttcca cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg
     1141 tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc
     1201 tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct
     1261 gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc
     1321 tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg
     1381 gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg
     1441 cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg
     1501 cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca
     1561 ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg
     1621 aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt
     1681 ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc
     1741 gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg
     1801 atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg
     1861 taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag
     1921 acagtggttc aaagtttttt tcttccattt caggtgtcgt gaggctagca tcgattgatc
     1981 aacaagtttg tacaaaaaag ttggcaccat gatagatccc gtcgttttac aacgtcgtga
     2041 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     2101 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     2161 tggcgaatgg cgctttgcct ggtttccggc accagaagcg gtgccggaaa gctggctgga
     2221 gtgcgatctt cctgaggccg atactgtcgt cgtcccctca aactggcaga tgcacggtta
     2281 cgatgcgccc atctacacca acgtgaccta tcccattacg gtcaatccgc cgtttgttcc
     2341 cacggagaat ccgacgggtt gttactcgct cacatttaat gttgatgaaa gctggctaca
     2401 ggaaggccag acgcgaatta tttttgatgg cgttaactcg gcgtttcatc tgtggtgcaa
     2461 cgggcgctgg gtcggttacg gccaggacag tcgtttgccg tctgaatttg acctgagcgc
     2521 atttttacgc gccggagaaa accgcctcgc ggtgatggtg ctgcgctgga gtgacggcag
     2581 ttatctggaa gatcaggata tgtggcggat gagcggcatt ttccgtgacg tctcgttgct
     2641 gcataaaccg actacacaaa tcagcgattt ccatgttgcc actcgcttta atgatgattt
     2701 cagccgcgct gtactggagg ctgaagttca gatgtgcggc gagttgcgtg actacctacg
     2761 ggtaacagtt tctttatggc agggtgaaac gcaggtcgcc agcggcaccg cgcctttcgg
     2821 cggtgaaatt atcgatgagc gtggtggtta tgccgatcgc gtcacactac gtctgaacgt
     2881 cgaaaacccg aaactgtgga gcgccgaaat cccgaatctc tatcgtgcgg tggttgaact
     2941 gcacaccgcc gacggcacgc tgattgaagc agaagcctgc gatgtcggtt tccgcgaggt
     3001 gcggattgaa aatggtctgc tgctgctgaa cggcaagccg ttgctgattc gaggcgttaa
     3061 ccgtcacgag catcatcctc tgcatggtca ggtcatggat gagcagacga tggtgcagga
     3121 tatcctgctg atgaagcaga acaactttaa cgccgtgcgc tgttcgcatt atccgaacca
     3181 tccgctgtgg tacacgctgt gcgaccgcta cggcctgtat gtggtggatg aagccaatat
     3241 tgaaacccac ggcatggtgc caatgaatcg tctgaccgat gatccgcgct ggctaccggc
     3301 gatgagcgaa cgcgtaacgc gaatggtgca gcgcgatcgt aatcacccga gtgtgatcat
     3361 ctggtcgctg gggaatgaat caggccacgg cgctaatcac gacgcgctgt atcgctggat
     3421 caaatctgtc gatccttccc gcccggtgca gtatgaaggc ggcggagccg acaccacggc
     3481 caccgatatt atttgcccga tgtacgcgcg cgtggatgaa gaccagccct tcccggctgt
     3541 gccgaaatgg tccatcaaaa aatggctttc gctacctgga gagacgcgcc cgctgatcct
     3601 ttgcgaatac gcccacgcga tgggtaacag tcttggcggt ttcgctaaat actggcaggc
     3661 gtttcgtcag tatccccgtt tacagggcgg cttcgtctgg gactgggtgg atcagtcgct
     3721 gattaaatat gatgaaaacg gcaacccgtg gtcggcttac ggcggtgatt ttggcgatac
     3781 gccgaacgat cgccagttct gtatgaacgg tctggtcttt gccgaccgca cgccgcatcc
     3841 agcgctgacg gaagcaaaac accagcagca gtttttccag ttccgtttat ccgggcaaac
     3901 catcgaagtg accagcgaat acctgttccg tcatagcgat aacgagctcc tgcactggat
     3961 ggtggcgctg gatggtaagc cgctggcaag cggtgaagtg cctctggatg tcgctccaca
     4021 aggtaaacag ttgattgaac tgcctgaact accgcagccg gagagcgccg ggcaactctg
     4081 gctcacagta cgcgtagtgc aaccgaacgc gaccgcatgg tcagaagccg gccacatcag
     4141 cgcctggcag cagtggcgtc tggcggaaaa cctcagtgtg acgctccccg ccgcgtccca
     4201 cgccatcccg catctgacca ccagcgaaat ggatttttgc atcgagctgg gtaataagcg
     4261 ttggcaattt aaccgccagt caggctttct ttcacagatg tggattggcg ataaaaaaca
     4321 actgctgacg ccgctgcgcg atcagttcac ccgtgcaccg ctggataacg acattggcgt
     4381 aagtgaagcg acccgcattg accctaacgc ctgggtcgaa cgctggaagg cggcgggcca
     4441 ttaccaggcc gaagcagcgt tgttgcagtg cacggcagat acacttgctg acgcggtgct
     4501 gattacgacc gctcacgcgt ggcagcatca ggggaaaacc ttatttatca gccggaaaac
     4561 ctaccggatt gatggtagtg gtcaaatggc gattaccgtt gatgttgaag tggcgagcga
     4621 tacaccgcat ccggcgcgga ttggcctgaa ctgccagctg gcgcaggtag cagagcgggt
     4681 aaactggctc ggattagggc cgcaagaaaa ctatcccgac cgccttactg ccgcctgttt
     4741 tgaccgctgg gatctgccat tgtcagacat gtataccccg tacgtcttcc cgagcgaaaa
     4801 cggtctgcgc tgcgggacgc gcgaattgaa ttatggccca caccagtggc gcggcgactt
     4861 ccagttcaac atcagccgct acagtcaaca gcaactgatg gaaaccagcc atcgccatct
     4921 gctgcacgcg gaagaaggca catggctgaa tatcgacggt ttccatatgg ggattggtgg
     4981 agacgactcc tggagcccgt cagtatcggc ggaattacag ctgagcgccg gtcgctacca
     5041 ttaccagttg gtctggtgtc aaaaaactaa gggtgggcgc gccgacccag ctttcttgta
     5101 caaagtggtt gatatccagc acagtggcgg ccgctcgagt ctagagggcc cgcggttcga
     5161 aggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg gtttgccaac
     5221 tttcttgtac aaagtggttg atatcggtaa gcctatccct aaccctctcc tcggtctcga
     5281 ttctacgtag taatgaacta gtaccggtta agtcgacaat caacgcgtta agtcgacaat
     5341 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     5401 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     5461 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     5521 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     5581 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     5641 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     5701 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     5761 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     5821 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     5881 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac
     5941 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     6001 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     6061 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     6121 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     6181 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtacgtata
     6241 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     6301 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     6361 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     6421 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     6481 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     6541 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     6601 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     6661 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     6721 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     6781 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     6841 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     6901 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     6961 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     7021 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     7081 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     7141 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     7201 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     7261 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     7321 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     7381 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     7441 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     7501 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     7561 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     7621 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     7681 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     7741 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     7801 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     7861 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     7921 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     7981 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     8041 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     8101 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     8161 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     8221 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     8281 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     8341 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     8401 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     8461 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     8521 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     8581 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     8641 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     8701 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     8761 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     8821 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     8881 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     8941 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     9001 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     9061 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     9121 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     9181 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     9241 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     9301 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     9361 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     9421 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
     9481 cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
     9541 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
     9601 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
     9661 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
     9721 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     9781 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     9841 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     9901 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
     9961 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
    10021 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
    10081 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
    10141 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
    10201 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
    10261 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
    10321 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
    10381 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
    10441 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
    10501 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
    10561 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
    10621 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
    10681 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
    10741 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
    10801 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
    10861 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
    10921 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
    10981 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
    11041 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
    11101 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
    11161 gtttcagacc cacctcccaa ccccgagggg acccatgcat tgcatctcaa ttagtcagca
    11221 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcgtctc
    11281 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
    11341 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
    11401 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
    11461 ttttgcaaaa agctttctag aggtaccacc ggagcttacc atgaccgagt acaagcccac
    11521 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
    11581 cgccgactac cccgccacgc gccacaccgt cgatccg
//
