LOCUS       sequence-234957-        9460 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Open reading frame vector encoding MCL1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9460)
  AUTHORS   Hong AL, Guerriero JL, Doshi MB, Kynnap BD, Kim WJ, Schinzel AC, 
            Modiste R, Schlauch AJ, Adam RM, Kwiatkowski DJ, Beroukhim R, Letai 
            A, Rosenberg JE, Hahn WC
  TITLE     MCL1 and DEDD promote urothelial carcinoma progression.
  JOURNAL   Mol Cancer Res. 2019 Feb 18. pii: 1541-7786.MCR-18-0963. doi: 
            10.1158/1541-7786.MCR-18-0963.
  PUBMED    30777879
REFERENCE   2  (bases 1 to 9460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cancer 
            Res. 2019 Feb 18. pii: 1541-7786.MCR-18-0963. doi: 
            10.1158/1541-7786.MCR-18-0963."
COMMENT     Sequence Label: pLX307 MCL1
FEATURES             Location/Qualifiers
     source          1..9460
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        27..237
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        49..245
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      96..231
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             277..879
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(277..296)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     773..793
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    922..1039
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1177..2355
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1408..2346
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2303..2323
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    2375..2399
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3450..3474)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3482..3523
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3574..4162
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3627..3647)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4045..4056)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4234..4467
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4545..4666
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4582..4601)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4636..4655
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4706..4841
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4861..4880)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4862..4880)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4890..4907)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4890..4906)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4899..4921)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5048..5503
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5135..5154)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5345..5366
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5529..5633
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5634..5702,5703..6494)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5852..5871)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6665..7253
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7154..7173
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7407..7424
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7541..7562
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7577..7582,7583..7600,7601..7607)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7615..7631
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7620..7642
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7639..7655
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7639..7655
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7674..7694
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7676..7694
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7722..7948
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7949..8129
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8176..8301
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8794..9027
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9212..9256
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg cgtctcaatt
       61 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
      121 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
      181 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
      241 gcaaaaagct ttctagaggt accaccggag cttaccatga ccgagtacaa gcccacggtg
      301 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
      361 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
      421 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
      481 gacggcgccg ccgtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
      541 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
      601 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
      661 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
      721 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
      781 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
      841 cgcacctggt gcatgacccg caagcccggt gccagttaac gaattctaga tcttgagaca
      901 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
      961 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     1021 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac tttggcgccg
     1081 gctcgaggcc tgcaggtgca aagatggata aagttttaaa cagagaggaa tctttgcagc
     1141 taatggacct tctaggtctt gaaaggagtg ggaattggct ccggtgcccg tcagtgggca
     1201 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt
     1261 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
     1321 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
     1381 cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc
     1441 ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg
     1501 attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa
     1561 ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg
     1621 cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa
     1681 aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc
     1741 caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg
     1801 tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg
     1861 gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc
     1921 ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg
     1981 cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg
     2041 ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac
     2101 tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg
     2161 tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg
     2221 gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt
     2281 gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca
     2341 tttcaggtgt cgtgaggcta gcatcgattg atcaacaagt ttgtacaaaa aagttggcat
     2401 gtttggcctc aaaagaaacg cggtaatcgg actcaacctc tactgtgggg gggccggctt
     2461 gggggccggc agcggcggcg ccacccgccc gggagggcga cttttggcta cggagaagga
     2521 ggcctcggcc cggcgagaga tagggggagg ggaggccggc gcggtgattg gcggaagcgc
     2581 cggcgcaagc cccccgtcca ccctcacgcc agactcccgg agggtcgcgc ggccgccgcc
     2641 cattggcgcc gaggtccccg acgtcaccgc gacccccgcg aggctgcttt tcttcgcgcc
     2701 cacccgccgc gcggcgccgc ttgaggagat ggaagccccg gccgctgacg ccatcatgtc
     2761 gcccgaagag gagctggacg ggtacgagcc ggagcctctc gggaagcggc cggctgtcct
     2821 gccgctgctg gagttggtcg gggaatctgg taataacacc agtacggacg ggtcactacc
     2881 ctcgacgccg ccgccagcag aggaggagga ggacgagttg taccggcagt cgctggagat
     2941 tatctctcgg taccttcggg agcaggccac cggcgccaag gacacaaagc caatgggcag
     3001 gtctggggcc accagcagga aggcgctgga gaccttacga cgggttgggg atggcgtgca
     3061 gcgcaaccac gagacggcct tccaaggcat gcttcggaaa ctggacatca aaaacgaaga
     3121 cgatgtgaaa tcgttgtctc gagtgatgat ccatgttttc agcgacggcg taacaaactg
     3181 gggcaggatt gtgactctca tttcttttgg tgcctttgtg gctaaacact tgaagaccat
     3241 aaaccaagaa agctgcatcg aaccattagc agaaagtatc acagacgttc tcgtaaggac
     3301 aaaacgggac tggctagtta aacaaagagg ctgggatggg tttgtggagt tcttccatgt
     3361 agaggaccta gaaggtggca tcaggaatgt gctgctggct tttgcaggtg ttgctggagt
     3421 aggagctggt ttggcatatc taataagata cccaactttc ttgtacaaag tggttgatat
     3481 cggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat gaactagtac
     3541 cggttaagtc gacaatcaac gcgttaagtc gacaatcaac ctctggatta caaaatttgt
     3601 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     3661 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     3721 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     3781 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     3841 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     3901 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     3961 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     4021 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     4081 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     4141 tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag
     4201 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     4261 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     4321 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     4381 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     4441 tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc
     4501 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     4561 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     4621 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     4681 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     4741 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     4801 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     4861 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     4921 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     4981 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     5041 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     5101 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     5161 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     5221 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     5281 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     5341 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     5401 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     5461 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     5521 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     5581 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     5641 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     5701 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     5761 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     5821 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     5881 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     5941 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     6001 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     6061 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     6121 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     6181 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     6241 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     6301 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     6361 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     6421 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     6481 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     6541 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     6601 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     6661 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     6721 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     6781 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     6841 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     6901 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     6961 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     7021 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     7081 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     7141 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     7201 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     7261 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     7321 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     7381 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     7441 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     7501 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     7561 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     7621 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
     7681 accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact
     7741 cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa
     7801 gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca
     7861 acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt
     7921 atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg
     7981 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     8041 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     8101 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     8161 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     8221 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     8281 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     8341 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     8401 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     8461 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     8521 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     8581 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     8641 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     8701 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     8761 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     8821 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     8881 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     8941 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     9001 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     9061 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     9121 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     9181 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     9241 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     9301 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     9361 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     9421 gaggggaccc atgcattgca tctcaattag tcagcaacca
//
