LOCUS       sequence-235014-        5638 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of HA tagged hnRNPK-lambdaN fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5638)
  AUTHORS   Lubelsky Y, Ulitsky I
  TITLE     Sequences enriched in Alu repeats drive nuclear localization of long
            RNAs in human cells.
  JOURNAL   Nature. 2018 Mar 1;555(7694):107-111. doi: 10.1038/nature25757. Epub
            2018 Jan 24.
  PUBMED    29466324
REFERENCE   2  (bases 1 to 5638)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature25757"; journalName: "Nature"; date: "2018-03-1- 1"; 
            volume: "555"; issue: "7694"; pages: "107-111"
COMMENT     Sequence Label: NHA-hnRNPK
FEATURES             Location/Qualifiers
     source          1..5638
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..576
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     527..547
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     621..640
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        621..639
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             676..741
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             778..804
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    2417..2538
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2454..2473)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2508..2527
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2545..3000)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2682..2703)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2894..2913
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3027..3131
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3133..3490
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3133..3324
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3156..3176)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3341..3476
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3541..4335
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3595..3614)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4205..4224
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4399..4447
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(4488..4507)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      4942..5530
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5431..5450
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtccgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta cgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacaccaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactgcga tcgcccgccc cgttgacgca aatgggcggt
      541 aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag agaacccact
      601 gcttactggc ttatcgaaat taatacgact cactataggg agacccaagc tctagcgcta
      661 ccggtcgcca ccatgatgga cgcacaaaca cgacgacgtg agcgtcgcgc tgagaaacaa
      721 gctcaatgga aagctgcaaa cccaccgctc gaggctgccc aagcttacac catggcctac
      781 ccatatgatg ttccagatta cgctatggaa actgaacagc cagaagaaac cttccctaac
      841 actgaaacca atggtgaatt tggtaaacgc cctgcagaag atatggaaga ggaacaagca
      901 tttaaaagat ctagaaacac tgatgagatg gttgaattac gcattctgct tcagagcaag
      961 aatgctgggg cagtgattgg aaaaggaggc aagaatatta aggctctccg tacagactac
     1021 aatgccagtg tttcagtccc agacagcagt ggccccgagc gcatattgag tatcagtgct
     1081 gatattgaaa caattggaga aattctgaag aaaatcatcc ctaccttgga agagggcctg
     1141 cagttgccat cacccactgc aaccagccag ctcccgctcg aatctgatgc tgtggaatgc
     1201 ttaaattacc aacactataa aggaagtgac tttgactgcg agttgaggct gttgattcat
     1261 cagagtctag caggaggaat tattggggtc aaaggtgcta aaatcaaaga acttcgagag
     1321 aacactcaaa ccaccatcaa gcttttccag gaatgctgtc ctcattccac tgacagagtt
     1381 gttcttattg gaggaaaacc cgatagggtt gtagagtgca taaagatcat ccttgatctt
     1441 atatctgagt ctcccatcaa aggacgtgca cagccttatg atcccaattt ttacgatgaa
     1501 acctatgatt atggtggttt tacaatgatg tttgatgacc gtcgcggacg cccagtggga
     1561 tttcccatgc ggggaagagg tggttttgac agaatgcctc ctggtcgggg tgggcgtccc
     1621 atgcctccat ctagaagaga ttatgatgat atgagccctc gtcgaggacc acctccccct
     1681 cctcccggac gaggcggccg gggtggtagc agagctcgga atcttcctct tcctccacca
     1741 ccaccaccta gagggggaga cctcatggcc tatgacagaa gagggagacc tggagaccgt
     1801 tacgacggca tggttggttt cagtgctgat gaaacttggg actctgcaat agatacatgg
     1861 agcccatcag aatggcagat ggcttatgaa ccacagggtg gctccggata tgattattcc
     1921 tatgcagggg gtcgtggctc atatggtgat cttggtggac ctattattac tacacaagta
     1981 actattccca aagatttggc tggatctatt attggcaaag gtggtcagcg gattaaacaa
     2041 atccgtcatg agtcgggagc ttcgatcaaa attgatgagc ctttagaagg atccgaagat
     2101 cggatcatta ccattacagg aacacaggac cagatacaga atgcacagta tttgctgcag
     2161 aacagtgtga agcagtatgc agatgttgaa ggattctaat gcaagatatt ttttcttttt
     2221 tatagtgtga agcagtattc tggaaagttt ttctaagaat tctgcagtcg acggtaccgc
     2281 gggcccggga tccaccggat ctagataact gatcataatc agccatacca catttgtaga
     2341 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     2401 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     2461 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     2521 actcatcaat gtatcttaac gcgtaaattg taagcgttaa tattttgtta aaattcgcgt
     2581 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     2641 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     2701 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     2761 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     2821 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     2881 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     2941 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     3001 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     3061 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     3121 aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg
     3181 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     3241 aaccatagtc cgaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     3301 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     3361 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     3421 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     3481 cttttgcaaa aagcttgatt cttctgacac aacagtctcg aacttaaggc tagagccacc
     3541 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     3601 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     3661 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     3721 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     3781 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     3841 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     3901 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     3961 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     4021 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
     4081 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     4141 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     4201 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     4261 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     4321 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
     4381 tgccatcacg atggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt
     4441 ttgtgtgaat cgatagcgat aaggatccgc gtatggtgca ctctcagtac aatctgctct
     4501 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     4561 gcttgtctgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     4621 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     4681 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     4741 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     4801 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     4861 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     4921 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     4981 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     5041 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     5101 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     5161 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     5221 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     5281 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     5341 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     5401 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     5461 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     5521 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     5581 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcc
//
