LOCUS       sequence-235021-        7150 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Allows for body-wall muscle expression of HPO-30.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7150)
  AUTHORS   Sharma P, Li L, Liu H, Tikiyani V, Hu Z, Babu K
  TITLE     The Claudin-like Protein HPO-30 Is Required to Maintain LAChRs at 
            the C. elegans Neuromuscular Junction.
  JOURNAL   J Neurosci. 2018 Aug 8;38(32):7072-7087. doi: 
            10.1523/JNEUROSCI.3487-17.2018. Epub 2018 Jun 27.
  PUBMED    29950505
REFERENCE   2  (bases 1 to 7150)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.3487-17.2018"; journalName: "J Neurosci"; date: 
            "2018-08-8- 8"; volume: "38"; issue: "32"; pages: "7072-7087"
COMMENT     Sequence Label: Pmyo-3::hpo-30 gDNA (pBAB204)
FEATURES             Location/Qualifiers
     source          1..7150
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(81..100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(760..1551,1552..1620))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1383..1402
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1621..1725)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1793..1811
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6749..6765)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6749..6765)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6762..6784)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6773..6789
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6797..6803,6804..6821,6822..6827))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6842..6863
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6980..6997)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      661 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      721 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
      781 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
      841 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
      901 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
      961 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     1021 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     1081 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     1141 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     1201 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     1261 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     1321 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     1381 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     1441 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     1501 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     1561 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     1621 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     1681 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     1741 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     1801 tatcacgagg cccttaagag tgattatagt ctctgttttc gttaattttg aattttgctt
     1861 gataaggctg caacaaagat caggttgaca tatattttca gtaatttatt ttaacctgta
     1921 ctctatcact gccggctata ataagttctt gaataaaata attttcccga caaaacatga
     1981 gtatttcttt cgaaaataaa agtgcaggct aattagagat tattctgtaa ttaactgcat
     2041 aatttgtcac gtgccatagt tttacattcc actacgtcat agttcttaaa atactaatct
     2101 cctgaaaata gaagtaggtg aagaaagttt aattatcagt tctaaaatga caattgatct
     2161 ttggaatatg ttctgaaact accgatcatt gaacagatgc tatttgaatg atatagaatt
     2221 gtatatttgc aatttctgaa acgcgttctt aaaggcacac agattaattc aaaagggtct
     2281 ggccgcaaaa aggtttatgg tggccgattt tgagttttgt gtgtgattgc tttttcacaa
     2341 tcagtgtttt caggattatg tgatgaacta gatcttcaag tttcgttaca tttcatatgt
     2401 tttcggaact cacgaagtac atattgggta ttgtgctcaa aaaattcagc aatcagcttc
     2461 gctccgctga ctttagaacc caaaaaaata gtatggccaa actgactgtg ttacgatcat
     2521 ttcaattttt caatacatat ttaagatttc taagagtaag aaggtcaaaa actgttctgg
     2581 aatacatata tatttttcag gttacaatta gtcaaaaagt gcactgaaat atacgtttta
     2641 atttcacgaa taacccaatt agttcaatgt atttttggtc aaccaacgtt aaagtttggc
     2701 ttccaaccaa ttatcatttc tgatcaacca caatgttttt tctttatctg caagttaatt
     2761 ttttattttt atccagatgt ttggcatatt tttcaattct tcactagcgc ccacttcttg
     2821 cacttccggc gccctgaatc taatgcatct gttgcaagaa ttgaaagacc aatcaacaca
     2881 ttgttttctt cacgagatac tgaagaaaat gaataaaaac agagaaaaag agccatgtga
     2941 ttagtgacaa ctgttgctaa cagataccat agcttggact tggtacgtga tggcaacgta
     3001 tgggtcaaca aaaatgattg cagagggggt gcaaaacagt caagtcgaga aaatatgaaa
     3061 aacagaaaac aaagaacaga aaaatgggtt tgagagtcag tataatttat aaaagaaaaa
     3121 ttgtacatag aaattaacca tttttgtaga agaagttatt tttcaagcat cgttaaaaat
     3181 tattcaaagc accttatttc atatttaatt ttaaacatgg ttaaatgaac aacacggtgc
     3241 gcaatcagga aaacttgaaa tctgaaactg ttgttgtgat cttcttcgca actgttcaga
     3301 tagcactagt gtaatgttaa gagtgcgcga atataatgga atataatgga tcacacctcc
     3361 tgccatcagg taaacgtctc tgttatcaca tatttccaac tattaaattt ttacctttta
     3421 cagttttaca tttttttgaa aaaagtaact ttttgtcttc aaaatccctg acgaaaatat
     3481 caaatatttt aatcgagact gcagaggaac cgattgatga tttggaaaat ccagctttac
     3541 ctgtgtaaga actgaaaagt ttcataaccc tagggtattc ccagttacat tccccactgg
     3601 ctaacaatag cacccagttt ttcatcacct ttcttcaaat ttctcggcga tttgttaaaa
     3661 acaaaatttg tgtcccttct ctgatatctc tatgtctcta aacacaagtt catcggaaaa
     3721 cgaaggaggg taggtgttgg ttgggctccc gaagtgaaaa tagaagagca agaatagaat
     3781 attagagaga gagtgcagag agggcgggat agctcccggg attccgtttt cttcttcttt
     3841 atcttcaacg atgatgtgtg tgcgtgttgt atagattctg ttgctccccc acaactcgct
     3901 ccgaaggctc aatacaattc aattgatatt ggaggagagc ctaccggagt gggaggataa
     3961 gaagaaacat aagaagaaga agaagaagaa gcatgcttct ggtttttgat gctatgaaaa
     4021 cggcacaaaa agatgattga ggtccctttt caataccttc tctcatcttt caaatcccat
     4081 tgaaacctaa aacttctcac cacgctttac cattgttctc caaaaactta tagcaatgtc
     4141 tataactttt ttatctctga aaagcagtgt tccatttttc tttttcctat tttatttcaa
     4201 ttgtttctca catttcgttt ggattctttg cttgtcaacc agcttcttct tccactttta
     4261 ccgtctaatt ttcagggcag ggagccatca aacccacgac cactagatcc atctagaaat
     4321 gctcgagatg ccattaatta tgtacaaatt tctattagtc acttcaatat tcttaatcgt
     4381 ttccgggcta atattaactg cgttctcgtt gtttagtcct ctttgggaag taagtttcta
     4441 gtcaataaaa ttctggtttt ttttaaatgt caattttgta ggtagttgat tttcctcgtt
     4501 ctcatttatc acatcatcac ggtctttggt gggattgtat tgtacatcat gaaactttaa
     4561 ttccgttaca tgaagatcaa gcagaacttc gaggtacctt ttttctgctt ttgttttcta
     4621 aataaaacaa gtttaaggtg atcgatgtga tagtaagatg gattcttcgg ttcaagcatc
     4681 attaagagtt gctcttgaaa aaggagatga agaggccaga gagcttttgt tacatcggtt
     4741 tctacgtaag ttaacaatat tttaatgctt ttcatgtgcc gaaacttact tttttctttg
     4801 ttagaaagat aaactagaaa ctatataaaa ctgatttacc aaatgcaggt tcaagttatt
     4861 ttttaaaata attagagtgt gagcgtagta gttcaataat ttgttgatca attttctctt
     4921 tctggataca gaagcgtatt ggcattgcaa cgtacataca catgtttggt tccatgctat
     4981 taattcaatt gattttctgt cttaaattct aggactgtaa tgctattttt tgtaatttag
     5041 tattttctga tctagaatat gcggtaacaa taaattagat tttactgtcc aaacacaaaa
     5101 ttactgaaga tacaagttga ttttatattc ttcatgaaac caaaaaaaaa ttaatttcag
     5161 ctcatcacaa aggtgtaatt ttcttcgcag ttttcacttt tgttttcgga ctaatctcca
     5221 tcctgattgg ctcctgttct ccatgttttc caccaaatgc actactttat gtagttggtg
     5281 tctttatgac cggagcatgt tctttattag cagatattat ctacatattt gcttttaatc
     5341 agaaaccaat ttttacaaaa gaacaatctg agccacacca agaagtttta tcgagaagag
     5401 aacgaggatc cattggaccc atttatgtaa aaattcgaaa aaaaattgat gtcatctttt
     5461 tatttttttt ttcaaattcc agaagcgtct tggcattgca acgtacatgc atatgtttgg
     5521 ttccatgcta ttaattgctg cattcatctt ttcaattttt tgtgcctact tcctcatcac
     5581 ttcaaaacat gcacacgacg tttgttgcac aagtcgaaaa gagtacagag agcagacaaa
     5641 gtaagtttaa tctgatttgc ggatttttct aaacatgtaa ctttcgtcta ataaaacaac
     5701 tttcaagagt aaaataaagg ttttcagatg gaaaaacaat gggctaattc taaaaactgg
     5761 aagagtaaat catcaatcac atcggccatt tgtagtgatt gacgatgaca gcagtatgtg
     5821 aactagtaga aacagttatg tttggtatat tgggaatgta ttctgtcatt taaggcaaaa
     5881 accccataga cactactcca ctttcaaatt atcaacttct actcaccttc cactgagcct
     5941 caaacccaaa ccttcttccg atctttcttg catcgtgctc atcaatactt gtgattttag
     6001 caagaaaaac gccgtcctcg aaatcttgac aaacaatagg ggaaacccca aaaagcaaaa
     6061 gcaggaaaat taatttcata aactttcact tgattgaaaa attccaaaaa tactccaaaa
     6121 ctttttcacg atgagcatga tttgacgtca tgagaggccc agacgtgcga agaaataaaa
     6181 attgcggtca taaactgaaa cgtaacatat gataaggtat tttgtgtgcg gttttttcta
     6241 tgatgtttca aaaaaattta tcagaagtaa aaaacataag aagtgtacac agaacattgt
     6301 gtagattttg ggatagaatt attattaatg gggggaggga gcacaatttt tttcgacttt
     6361 ttattacgaa ttaattctat ttttgaatct acacaatttc attgttagag gtgacttaaa
     6421 agaagctaaa aaacaaagaa attaagagaa gttttttgat aataacaaaa atagggggtg
     6481 ggagcacagg gagaaagagc atgtagggat gttgaagagt aattggactt agaagtcaga
     6541 ggcacgggcg cgagatggcg atctgatgac agcggccgat gcggagctca gatatcaata
     6601 ccatggtacc gtcgacgcta gccaagggtc ctcctgaaaa tgttctatgt tatgttagta
     6661 tcattcgaaa catacctttg ggtcctttgg ccaatcccgg ggatcctcta gagtcgacct
     6721 gcaggcatgc aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     6781 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     6841 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     6901 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     6961 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     7021 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     7081 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     7141 tgctggcgtt
//
