LOCUS       sequence-235146-       12433 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the BaL HIV-1 isolate; 
            C-terminal truncation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12433)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 12433)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-BaLgp160-754*
FEATURES             Location/Qualifiers
     source          1..12433
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1948..1992
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     polyA_signal    2270..2404
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2294..2313)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2348..2367
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2547..2569)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      3129..4918
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(5220..7145)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(7580..7598)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7666..7770
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7771..7839,7840..8631)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7989..8008)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8802..9390
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9291..9310
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        9821..9966
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             10009..11046
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(11044..11063)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11088..11136
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        11790..12169
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12170..12373
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     12324..12344
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     12370..12394
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gccaccatgc ccatggggag cttgcaaccg ctcgccactc tctatctcct cggcatgttg
       61 gtagcatctg tgctggctga ggaaaagctc tgggtgactg tgtattacgg ggtcccagtt
      121 tggaaggagg ctactaccac gctcttctgc gcctccgacc ggaaggcata cgacaccgag
      181 gtgcataatg tgtgggccac acacgcctgt gtgcctaccg accccaaccc acaggaagtg
      241 gaactgaaga acgtgactga gaatttcaat atgtggaaaa ataatatggt ggagcagatg
      301 cacgaggaca ttattagcct ctgggatcag tcccttaaac cctgcgtgaa actgacaccg
      361 ctgtgcgtaa cgctgaattg tactgacctc cggaatgcca cgaacggaaa cgacacaaat
      421 accacgtcaa gctcccgagg gatggtggga gggggagaaa tgaagaactg ctcattcaat
      481 ataacgacga atatccgagg gaaggttcag aaagagtatg ccctgtttta caaactggac
      541 atcgctccta tcgacaacaa cagcaataac aggtaccgcc tgattagctg caacacttct
      601 gtgattaccc aggcctgccc caaggtgagc ttcgagccta tcccaatcca ctactgcgcc
      661 ccagcaggct ttgccatcct gaaatgcaag gacaagaagt tcaacggcaa aggcccatgt
      721 acaaatgtca gtacagtaca gtgcacacac ggtatccgcc cagttgtctc tacacaactg
      781 ctgctgaatg gatccttggc agaggaagag gtggtgatca gatctgctaa cttcgccgat
      841 aatgctaaag tcatcattgt tcagctgaac gagtctgtcg aaatcaattg tactcgccca
      901 aacaacaaca cgcgcaagag cattcatatc ggaccgggca gggcctttta cacaacaggg
      961 gagattattg gtgatattag acaagcccac tgcaacctca gtcgagcaaa gtggaatgat
     1021 actctcaaca aaattgttat caaattgcgg gaacagtttg ggaacaagac aatcgtattt
     1081 aagcactcat ccggaggcga tccagaaatc gtgacacact cttttaactg cggtggagaa
     1141 tttttctact gcaactccac ccagctgttc aacagtacgt ggaacgtgac agaggagtct
     1201 aacaacaccg tggagaataa tacgatcacc cttccatgtc ggattaagca gatcatcaat
     1261 atgtggcagg aggtgggaag agcaatgtac gcgccgccca ttaggggcca gatcagatgc
     1321 agttcaaata tcacaggcct gctcctgaca cgcgacggag gcccagagga caacaagaca
     1381 gaggtgttta ggcccggcgg cggcgacatg cgagataatt ggaggtctga attgtacaag
     1441 tacaaggtgg tgaaaatcga acccctggga gtggcgccta ctaaggcaaa gcgccgcgtt
     1501 gtgcagagag agaagagggc agtgggaatt ggggcggttt ttcttgggtt cctgggggct
     1561 gcaggtagca ccatgggagc ggccgctatg acactgacag tgcaagccag actcctgctg
     1621 tctggtattg tccaacagca aaacaatttg ctccgcgcaa tagaagccca gcagcacctg
     1681 ctccaactga ctgtgtgggg cattaaacaa ttgcaggctc gcgtgctggc agtcgaacgg
     1741 tacctgaggg atcaacagct cctgggcatc tggggctgct ccgggaaact catctgtacg
     1801 acagcggtcc cttggaatgc cagctggtcc aacaaaagcc tcaacaagat ttgggacaat
     1861 atgacgtgga ttgagtggga cagggaaatt aacaattata cctcaatcat atattctctg
     1921 attgaagagt cccagaatca gcaagagaag aatgagcagg aattgttgga acttgataaa
     1981 tgggcaagcc tgtggaactg gtttgacata accaaatggt tgtggtatat caaaatcttc
     2041 ataatgatcg tcggcggact catcgggctc cgaattgtgt tctcagtcct cagcattgtg
     2101 aatagggtgc gccagggcta ttctcccctg agcttccaga cccatctgcc ctctagccga
     2161 ggaccagata ggcctggtgg gatcgaggaa gaaggcgggg aacgggacag ggataggagt
     2221 ggtcctcttg ttaacgggtt cctgtgataa ctcgaggccg gcaaggccgg atccagacat
     2281 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     2341 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     2401 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
     2461 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc cggctgcctc
     2521 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     2581 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     2641 ggcgggtgtc ggggcgcagc catgaggtcg actctagagg atcgatcccc gccccggacg
     2701 aactaaacct gactacgaca tctctgcccc ttcttcgcgg ggcagtgcat gtaatccctt
     2761 cagttggttg gtacaacttg ccaactgggc cctgttccac atgtgacacg gggggggacc
     2821 aaacacaaag gggttctctg actgtagttg acatccttat aaatggatgt gcacatttgc
     2881 caacactgag tggctttcat cctggagcag actttgcagt ctgtggactg caacacaaca
     2941 ttgcctttat gtgtaactct tggctgaagc tcttacacca atgctggggg acatgtacct
     3001 cccaggggcc caggaagact acgggaggct acaccaacgt caatcagagg ggcctgtgta
     3061 gctaccgata agcggaccct caagagggca ttagcaatag tgtttataag gcccccttgt
     3121 taaccctaaa cgggtagcat atgcttcccg ggtagtagta tatactatcc agactaaccc
     3181 taattcaata gcatatgtta cccaacggga agcatatgct atcgaattag ggttagtaaa
     3241 agggtcctaa ggaacagcga tatctcccac cccatgagct gtcacggttt tatttacatg
     3301 gggtcaggat tccacgaggg tagtgaacca ttttagtcac aagggcagtg gctgaagatc
     3361 aaggagcggg cagtgaactc tcctgaatct tcgcctgctt cttcattctc cttcgtttag
     3421 ctaatagaat aactgctgag ttgtgaacag taaggtgtat gtgaggtgct cgaaaacaag
     3481 gtttcaggtg acgcccccag aataaaattt ggacgggggg ttcagtggtg gcattgtgct
     3541 atgacaccaa tataaccctc acaaacccct tgggcaataa atactagtgt aggaatgaaa
     3601 cattctgaat atctttaaca atagaaatcc atggggtggg gacaagccgt aaagactgga
     3661 tgtccatctc acacgaattt atggctatgg gcaacacata atcctagtgc aatatgatac
     3721 tggggttatt aagatgtgtc ccaggcaggg accaagacag gtgaaccatg ttgttacact
     3781 ctatttgtaa caaggggaaa gagagtggac gccgacagca gcggactcca ctggttgtct
     3841 ctaacacccc cgaaaattaa acggggctcc acgccaatgg ggcccataaa caaagacaag
     3901 tggccactct tttttttgaa attgtggagt gggggcacgc gtcagccccc acacgccgcc
     3961 ctgcggtttt ggactgtaaa ataagggtgt aataacttgg ctgattgtaa ccccgctaac
     4021 cactgcggtc aaaccacttg cccacaaaac cactaatggc accccgggga atacctgcat
     4081 aagtaggtgg gcgggccaag ataggggcgc gattgctgcg atctggagga caaattacac
     4141 acacttgcgc ctgagcgcca agcacagggt tgttggtcct catattcacg aggtcgctga
     4201 gagcacggtg ggctaatgtt gccatgggta gcatatacta cccaaatatc tggatagcat
     4261 atgctatcct aatctatatc tgggtagcat aggctatcct aatctatatc tgggtagcat
     4321 atgctatcct aatctatatc tgggtagtat atgctatcct aatttatatc tgggtagcat
     4381 aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat
     4441 atgctatcct aatctgtatc cgggtagcat atgctatcct aatagagatt agggtagtat
     4501 atgctatcct aatttatatc tgggtagcat atactaccca aatatctgga tagcatatgc
     4561 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagcataggc
     4621 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc
     4681 tatcctaatt tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc
     4741 tatcctaatc tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc
     4801 tatcctcatg catatacagt cagcatatga tacccagtag tagagtggga gtgctatcct
     4861 ttgcatatgc cgccacctcc caagggggcg tgaattttcg ctgcttgtcc ttttcctgct
     4921 ggttgctccc attcttaggt gaatttaagg aggccaggct aaagccgtcg catgtctgat
     4981 tgctcaccag gtaaatgtcg ctaatgtttt ccaacgcgag aaggtgttga gcgcggagct
     5041 gagtgacgtg acaacatggg tatgcccaat tgccccatgt tgggaggacg aaaatggtga
     5101 caagacagat ggccagaaat acaccaacag cacgcatgat gtctactggg gatttattct
     5161 ttagtgcggg ggaatacacg gcttttaata cgattgaggg cgtctcctaa caagttacat
     5221 cactcctgcc cttcctcacc ctcatctcca tcacctcctt catctccgtc atctccgtca
     5281 tcaccctccg cggcagcccc ttccaccata ggtggaaacc agggaggcaa atctactcca
     5341 tcgtcaaagc tgcacacagt caccctgata ttgcaggtag gagcgggctt tgtcataaca
     5401 aggtccttaa tcgcatcctt caaaacctca gcaaatatat gagtttgtaa aaagaccatg
     5461 aaataacaga caatggactc ccttagcggg ccaggttgtg ggccgggtcc aggggccatt
     5521 ccaaagggga gacgactcaa tggtgtaaga cgacattgtg gaatagcaag ggcagttcct
     5581 cgccttaggt tgtaaaggga ggtcttacta cctccatata cgaacacacc ggcgacccaa
     5641 gttccttcgt cggtagtcct ttctacgtga ctcctagcca ggagagctct taaaccttct
     5701 gcaatgttct caaatttcgg gttggaacct ccttgaccac gatgctttcc aaaccaccct
     5761 ccttttttgc gcctgcctcc atcaccctga ccccggggtc cagtgcttgg gccttctcct
     5821 gggtcatctg cggggccctg ctctatcgct cccgggggca cgtcaggctc accatctggg
     5881 ccaccttctt ggtggtattc aaaataatcg gcttccccta cagggtggaa aaatggcctt
     5941 ctacctggag ggggcctgcg cggtggagac ccggatgatg atgactgact actgggactc
     6001 ctgggcctct tttctccacg tccacgacct ctccccctgg ctctttcacg acttcccccc
     6061 ctggctcttt cacgtcctct accccggcgg cctccactac ctcctcgacc ccggcctcca
     6121 ctacctcctc gaccccggcc tccactgcct cctcgacccc ggcctccacc tcctgctcct
     6181 gcccctcctg ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc tgctcctgcc
     6241 cctcctgccc ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc tcctgcccct
     6301 cctcctgctc ctgcccctcc tgcccctcct cctgctcctg cccctcctgc ccctcctgct
     6361 cctgcccctc ctgcccctcc tgctcctgcc cctcctgccc ctcctgctcc tgcccctcct
     6421 gctcctgccc ctcctgctcc tgcccctcct gctcctgccc ctcctgcccc tcctgcccct
     6481 cctcctgctc ctgcccctcc tgctcctgcc cctcctgccc ctcctgcccc tcctgctcct
     6541 gcccctcctc ctgctcctgc ccctcctgcc cctcctgccc ctcctcctgc tcctgcccct
     6601 cctgcccctc ctcctgctcc tgcccctcct cctgctcctg cccctcctgc ccctcctgcc
     6661 cctcctcctg ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tcctgctcct
     6721 gcccctcctg cccctcctgc ccctcctcct gctcctgccc ctcctcctgc tcctgcccct
     6781 cctgcccctc ctgcccctcc tgcccctcct cctgctcctg cccctcctcc tgctcctgcc
     6841 cctcctgctc ctgcccctcc cgctcctgct cctgctcctg ttccaccgtg ggtccctttg
     6901 cagccaatgc aacttggacg tttttggggt ctccggacac catctctatg tcttggccct
     6961 gatcctgagc cgcccggggc tcctggtctt ccgcctcctc gtcctcgtcc tcttccccgt
     7021 cctcgtccat ggttatcacc ccctcttctt tgaggtccac tgccgccgga gccttctggt
     7081 ccagatgtgt ctcccttctc tcctaggcca tttccaggtc ctgtacctgg cccctcgtca
     7141 gacatgattc acactaaaag agatcaatag acatctttat tagacgacgc tcagtgaata
     7201 cagggagtgc agactcctgc cccctccaac agccccccca ccctcatccc cttcatggtc
     7261 gctgtcagac agatccaggt ctgaaaattc cccatcctcc gaaccatcct cgtcctcatc
     7321 accaattact cgcagcccgg aaaactcccg ctgaacatcc tcaagatttg cgtcctgagc
     7381 ctcaagccag gcctcaaatt cctcgtcccc ctttttgctg gacggtaggg atggggattc
     7441 tcgggacccc tcctcttcct cttcaaggtc accagacaga gatgctactg gggcaacgga
     7501 agaaaagctg ggtgcggcct gtgaggatca gcttatcgat gataagctgt caaacatgag
     7561 aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     7621 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     7681 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     7741 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     7801 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     7861 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     7921 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     7981 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
     8041 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     8101 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     8161 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     8221 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     8281 ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa
     8341 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     8401 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     8461 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     8521 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     8581 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     8641 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     8701 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     8761 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     8821 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     8881 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     8941 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     9001 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     9061 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     9121 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     9181 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     9241 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     9301 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     9361 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     9421 ctggcctttt gctggccttg aagctgtccc tgatggtcgt catctacctg cctggacagc
     9481 atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag
     9541 gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccccgaga
     9601 tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg
     9661 tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc
     9721 cgttagcgag gtgccgccct gcttcatccc cgtggcccgt tgctcgcgtt tgctggcggt
     9781 gtccccggaa gaaatatatt tgcatgtctt tagttctatg atgacacaaa ccccgcccag
     9841 cgtcttgtca ttggcgaatt cgaacacgca gatgcagtcg gggcggcgcg gtccgaggtc
     9901 cacttcgcat attaaggtga cgcgtgtggc ctcgaacacc gagcgaccct gcagcgaccc
     9961 gcttaacagc gtcaacagcg tgccgcagat cccggggggc aatgagatat gaaaaagcct
    10021 gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac
    10081 ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt
    10141 ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat
    10201 cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggaattcagc
    10261 gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct
    10321 gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg
    10381 gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac
    10441 actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact
    10501 gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg
    10561 gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc
    10621 ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat
    10681 tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag
    10741 cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg gctccgggcg
    10801 tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat
    10861 gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc
    10921 gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta
    10981 ctcgccgata gtggaaaccg acgccccagc actcgtccgg atcgggagat gggggaggct
    11041 aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag
    11101 acagaataaa acgcacgggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg
    11161 gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt
    11221 ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg
    11281 gggcggcagg ccctgccata gccactggcc ccgtgggtta gggacggggt cccccatggg
    11341 gaatggttta tggttcgtgg gggttattat tttgggcgtt gcgtggggtc aggtccacga
    11401 ctggactgag cagacagacc catggttttt ggatggcctg ggcatggacc gcatgtactg
    11461 gcgcgacacg aacaccgggc gtctgtggct gccaaacacc cccgaccccc aaaaaccacc
    11521 gcgcggattt ctggcgtgcc aagctagtcg accaattctc atgtttgaca gcttatcatc
    11581 gcagatccgg gcaacgttgt tgccattgct gcaggcgcag aactggtagg tatggaagat
    11641 ctatacattg aatcaatatt ggcaattagc catattagtc attggttata tagcataaat
    11701 caatattggc tattggccat tgcatacgtt gtatctatat cataatatgt acatttatat
    11761 tggctcatgt ccaatatgac cgccatgttg acattgatta ttgactagtt attaatagta
    11821 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
    11881 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
    11941 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
    12001 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagtc cgccccctat
    12061 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttacggga
    12121 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
    12181 ttggcagtac accaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
    12241 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
    12301 tcgtaataac cccgccccgt tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
    12361 tataagcaga gctcgtttag tgaaccgtca gatctctaga agctgggtac cagctgctag
    12421 caagcttgct agc
//
