LOCUS       sequence-235157-       12418 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the 25711 HIV-1 isolate; 
            C-terminal truncation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12418)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 12418)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-25711gp160-754*
FEATURES             Location/Qualifiers
     source          1..12418
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    2267..2401
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2291..2310)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2345..2364
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2544..2566)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      3126..4915
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(5217..7142)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(7577..7595)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7663..7767
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7768..7836,7837..8628)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7986..8005)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8799..9387
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9288..9307
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        9818..9963
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             10006..11043
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(11041..11060)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11085..11133
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        11787..12166
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12167..12370
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     12321..12341
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     12367..12391
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gccaccatgc ctatggggag cctccaaccc ctggctacac tctatttgct tggtatgctc
       61 gtagccagtg tcttggctaa tctctgggtc acagtatatt atggtgtacc cgtatggaag
      121 gaagctaaga ccaccctctt ctgtgcgtct gacgcgaagg gctacgacaa ggaagttcac
      181 aatgtttggg ctactcacgc atgtgttccg actgatccca acccgcaaga gatgcctctg
      241 gagaatgtta ccgagaattt caatatgtgg gaaaatgaca tggtgaatca aatgcacgaa
      301 gatgtgatca gtctgtggga tgagtccttg aagccttgcg tcaaactgac ccctctgtgc
      361 gtaactttga actgcacgga tgttaataaa aatgtttcct cttccgacac ggataactac
      421 aaagagacta tgaaggagcg gaaaaattgc acgttcaata tgacgacaga gcttcgcgac
      481 aagaaccaga agaaatatgc ccttttctat aagctcgaca ttgtcccact ggatgataat
      541 gacaatgcgt cataccggct tataaattgc aatacgagca cgctgactca ggcctgccca
      601 aaggttagtt ttgaccctat accgatccac tactgtgccc ctgcagggta cgcaattctc
      661 aaatgcaaga ataaaacctt caatgggata gggccgtgta acaaggtttc aaccgttcaa
      721 tgcacgcatg gaataaagcc ggtggtatct acacaacttt tgcttaacgg gagtcttgcg
      781 gaagaggaca ttgttattcg ctcagagaac ataaccgata atgcgaaaac aataatcgta
      841 cacctcaatg agtccgtgga gatcgtttgc atccgcccta ataataacac tcggaaatct
      901 attaggatag gtccaggcca aaccttttac gcgacgggtg atatagttgg ggacatccgc
      961 caggcatact gcaatatctc agaggggaaa tggaataaga ctctccagcg ggtatctgag
     1021 aagctggctg agcacttccc gaacagtaca attaacttca atagctcaag cgggggtgac
     1081 ctggagatta cgacgcattc tttcaattgt ggcggggaat ttttctattg caatacgtct
     1141 ggcctcttca acggaaccta tatgaacaat gatacgaaga gcaatgacac aaagtctaac
     1201 agttcatcaa tcatcacgat cccatgtcgc attaagcaaa ttatcaacat gtggcaagaa
     1261 gtcggacgcg cggtatacgc accacccatc gccggtaaca ttacttgtaa atccaacatt
     1321 acgggcattc ttctgactag ggatggtggg agaggcgaag aagtgaaaaa tgataccgag
     1381 acatttaggc caggaggggg caacatgaaa gacaattgga ggagtgagct ttataaatac
     1441 aaagtagttg aaatcaagcc cctcggagtg gcaccaaccg ccgccaagcg cagagtcgtc
     1501 gagagagaaa agagggcagt aggacttggg gcggtgctcc ttggatttct tggggcagcc
     1561 gggagtacga tgggcgcagc ttccatcact ttgacggttc aggcgcgaca actcctctct
     1621 ggaattgttc aacagcagtc taacttgctc cgggccattg aggcgcagca gcacatgttg
     1681 cagctcaccg tgtggggtat taagcagctc caagctaggg tacttgccat cgagcggtat
     1741 cttaaggatc aacagctcct cggaatttgg gggtgttccg gtaagctcat ttgcacaaca
     1801 gcggtcccct ggaactccag ttggtctaat aagaatcaaa ctgagatatg ggacaagatg
     1861 acttggatgc aatgggatag agagatttca aattatacgg acactatata ccgccttctt
     1921 gaagatagcc aaaaccaaca ggaaaagaat gagaaggacc ttttggaact tgacaagtgg
     1981 cagaaccttt ggagttggtt caacataact aactggctct ggtacataag aatattcata
     2041 atgattgtag ggggcttgat aggcttgcga ataatattcg cggttctgtc aatcgtaaac
     2101 cgcgtacgac aaggatattc accgttgtct tttcagaccc tcgcgccgaa tcctcgggga
     2161 ttggaccgcc tggggcggat tgaagaagag ggagggaaag aggacaggaa cagatccatt
     2221 cgacttgtcc acggttttct ttgataactc gaggccggca aggccggatc cagacatgat
     2281 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     2341 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     2401 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
     2461 ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatccgg ctgcctcgcg
     2521 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     2581 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     2641 gggtgtcggg gcgcagccat gaggtcgact ctagaggatc gatccccgcc ccggacgaac
     2701 taaacctgac tacgacatct ctgccccttc ttcgcggggc agtgcatgta atcccttcag
     2761 ttggttggta caacttgcca actgggccct gttccacatg tgacacgggg ggggaccaaa
     2821 cacaaagggg ttctctgact gtagttgaca tccttataaa tggatgtgca catttgccaa
     2881 cactgagtgg ctttcatcct ggagcagact ttgcagtctg tggactgcaa cacaacattg
     2941 cctttatgtg taactcttgg ctgaagctct tacaccaatg ctgggggaca tgtacctccc
     3001 aggggcccag gaagactacg ggaggctaca ccaacgtcaa tcagaggggc ctgtgtagct
     3061 accgataagc ggaccctcaa gagggcatta gcaatagtgt ttataaggcc cccttgttaa
     3121 ccctaaacgg gtagcatatg cttcccgggt agtagtatat actatccaga ctaaccctaa
     3181 ttcaatagca tatgttaccc aacgggaagc atatgctatc gaattagggt tagtaaaagg
     3241 gtcctaagga acagcgatat ctcccacccc atgagctgtc acggttttat ttacatgggg
     3301 tcaggattcc acgagggtag tgaaccattt tagtcacaag ggcagtggct gaagatcaag
     3361 gagcgggcag tgaactctcc tgaatcttcg cctgcttctt cattctcctt cgtttagcta
     3421 atagaataac tgctgagttg tgaacagtaa ggtgtatgtg aggtgctcga aaacaaggtt
     3481 tcaggtgacg cccccagaat aaaatttgga cggggggttc agtggtggca ttgtgctatg
     3541 acaccaatat aaccctcaca aaccccttgg gcaataaata ctagtgtagg aatgaaacat
     3601 tctgaatatc tttaacaata gaaatccatg gggtggggac aagccgtaaa gactggatgt
     3661 ccatctcaca cgaatttatg gctatgggca acacataatc ctagtgcaat atgatactgg
     3721 ggttattaag atgtgtccca ggcagggacc aagacaggtg aaccatgttg ttacactcta
     3781 tttgtaacaa ggggaaagag agtggacgcc gacagcagcg gactccactg gttgtctcta
     3841 acacccccga aaattaaacg gggctccacg ccaatggggc ccataaacaa agacaagtgg
     3901 ccactctttt ttttgaaatt gtggagtggg ggcacgcgtc agcccccaca cgccgccctg
     3961 cggttttgga ctgtaaaata agggtgtaat aacttggctg attgtaaccc cgctaaccac
     4021 tgcggtcaaa ccacttgccc acaaaaccac taatggcacc ccggggaata cctgcataag
     4081 taggtgggcg ggccaagata ggggcgcgat tgctgcgatc tggaggacaa attacacaca
     4141 cttgcgcctg agcgccaagc acagggttgt tggtcctcat attcacgagg tcgctgagag
     4201 cacggtgggc taatgttgcc atgggtagca tatactaccc aaatatctgg atagcatatg
     4261 ctatcctaat ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg
     4321 ctatcctaat ctatatctgg gtagtatatg ctatcctaat ttatatctgg gtagcatagg
     4381 ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg
     4441 ctatcctaat ctgtatccgg gtagcatatg ctatcctaat agagattagg gtagtatatg
     4501 ctatcctaat ttatatctgg gtagcatata ctacccaaat atctggatag catatgctat
     4561 cctaatctat atctgggtag catatgctat cctaatctat atctgggtag cataggctat
     4621 cctaatctat atctgggtag catatgctat cctaatctat atctgggtag tatatgctat
     4681 cctaatttat atctgggtag cataggctat cctaatctat atctgggtag catatgctat
     4741 cctaatctat atctgggtag tatatgctat cctaatctgt atccgggtag catatgctat
     4801 cctcatgcat atacagtcag catatgatac ccagtagtag agtgggagtg ctatcctttg
     4861 catatgccgc cacctcccaa gggggcgtga attttcgctg cttgtccttt tcctgctggt
     4921 tgctcccatt cttaggtgaa tttaaggagg ccaggctaaa gccgtcgcat gtctgattgc
     4981 tcaccaggta aatgtcgcta atgttttcca acgcgagaag gtgttgagcg cggagctgag
     5041 tgacgtgaca acatgggtat gcccaattgc cccatgttgg gaggacgaaa atggtgacaa
     5101 gacagatggc cagaaataca ccaacagcac gcatgatgtc tactggggat ttattcttta
     5161 gtgcggggga atacacggct tttaatacga ttgagggcgt ctcctaacaa gttacatcac
     5221 tcctgccctt cctcaccctc atctccatca cctccttcat ctccgtcatc tccgtcatca
     5281 ccctccgcgg cagccccttc caccataggt ggaaaccagg gaggcaaatc tactccatcg
     5341 tcaaagctgc acacagtcac cctgatattg caggtaggag cgggctttgt cataacaagg
     5401 tccttaatcg catccttcaa aacctcagca aatatatgag tttgtaaaaa gaccatgaaa
     5461 taacagacaa tggactccct tagcgggcca ggttgtgggc cgggtccagg ggccattcca
     5521 aaggggagac gactcaatgg tgtaagacga cattgtggaa tagcaagggc agttcctcgc
     5581 cttaggttgt aaagggaggt cttactacct ccatatacga acacaccggc gacccaagtt
     5641 ccttcgtcgg tagtcctttc tacgtgactc ctagccagga gagctcttaa accttctgca
     5701 atgttctcaa atttcgggtt ggaacctcct tgaccacgat gctttccaaa ccaccctcct
     5761 tttttgcgcc tgcctccatc accctgaccc cggggtccag tgcttgggcc ttctcctggg
     5821 tcatctgcgg ggccctgctc tatcgctccc gggggcacgt caggctcacc atctgggcca
     5881 ccttcttggt ggtattcaaa ataatcggct tcccctacag ggtggaaaaa tggccttcta
     5941 cctggagggg gcctgcgcgg tggagacccg gatgatgatg actgactact gggactcctg
     6001 ggcctctttt ctccacgtcc acgacctctc cccctggctc tttcacgact tccccccctg
     6061 gctctttcac gtcctctacc ccggcggcct ccactacctc ctcgaccccg gcctccacta
     6121 cctcctcgac cccggcctcc actgcctcct cgaccccggc ctccacctcc tgctcctgcc
     6181 cctcctgctc ctgcccctcc tcctgctcct gcccctcctg cccctcctgc tcctgcccct
     6241 cctgcccctc ctgctcctgc ccctcctgcc cctcctgctc ctgcccctcc tgcccctcct
     6301 cctgctcctg cccctcctgc ccctcctcct gctcctgccc ctcctgcccc tcctgctcct
     6361 gcccctcctg cccctcctgc tcctgcccct cctgcccctc ctgctcctgc ccctcctgct
     6421 cctgcccctc ctgctcctgc ccctcctgct cctgcccctc ctgcccctcc tgcccctcct
     6481 cctgctcctg cccctcctgc tcctgcccct cctgcccctc ctgcccctcc tgctcctgcc
     6541 cctcctcctg ctcctgcccc tcctgcccct cctgcccctc ctcctgctcc tgcccctcct
     6601 gcccctcctc ctgctcctgc ccctcctcct gctcctgccc ctcctgcccc tcctgcccct
     6661 cctcctgctc ctgcccctcc tgcccctcct cctgctcctg cccctcctcc tgctcctgcc
     6721 cctcctgccc ctcctgcccc tcctcctgct cctgcccctc ctcctgctcc tgcccctcct
     6781 gcccctcctg cccctcctgc ccctcctcct gctcctgccc ctcctcctgc tcctgcccct
     6841 cctgctcctg cccctcccgc tcctgctcct gctcctgttc caccgtgggt ccctttgcag
     6901 ccaatgcaac ttggacgttt ttggggtctc cggacaccat ctctatgtct tggccctgat
     6961 cctgagccgc ccggggctcc tggtcttccg cctcctcgtc ctcgtcctct tccccgtcct
     7021 cgtccatggt tatcaccccc tcttctttga ggtccactgc cgccggagcc ttctggtcca
     7081 gatgtgtctc ccttctctcc taggccattt ccaggtcctg tacctggccc ctcgtcagac
     7141 atgattcaca ctaaaagaga tcaatagaca tctttattag acgacgctca gtgaatacag
     7201 ggagtgcaga ctcctgcccc ctccaacagc ccccccaccc tcatcccctt catggtcgct
     7261 gtcagacaga tccaggtctg aaaattcccc atcctccgaa ccatcctcgt cctcatcacc
     7321 aattactcgc agcccggaaa actcccgctg aacatcctca agatttgcgt cctgagcctc
     7381 aagccaggcc tcaaattcct cgtccccctt tttgctggac ggtagggatg gggattctcg
     7441 ggacccctcc tcttcctctt caaggtcacc agacagagat gctactgggg caacggaaga
     7501 aaagctgggt gcggcctgtg aggatcagct tatcgatgat aagctgtcaa acatgagaat
     7561 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     7621 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     7681 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     7741 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     7801 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     7861 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     7921 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     7981 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
     8041 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     8101 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     8161 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     8221 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     8281 ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta
     8341 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     8401 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     8461 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     8521 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     8581 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     8641 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     8701 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     8761 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     8821 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     8881 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     8941 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     9001 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     9061 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     9121 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     9181 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     9241 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     9301 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     9361 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     9421 gccttttgct ggccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg
     9481 gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc
     9541 atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cccgagatgc
     9601 gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt
     9661 gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt
     9721 tagcgaggtg ccgccctgct tcatccccgt ggcccgttgc tcgcgtttgc tggcggtgtc
     9781 cccggaagaa atatatttgc atgtctttag ttctatgatg acacaaaccc cgcccagcgt
     9841 cttgtcattg gcgaattcga acacgcagat gcagtcgggg cggcgcggtc cgaggtccac
     9901 ttcgcatatt aaggtgacgc gtgtggcctc gaacaccgag cgaccctgca gcgacccgct
     9961 taacagcgtc aacagcgtgc cgcagatccc ggggggcaat gagatatgaa aaagcctgaa
    10021 ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg
    10081 atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga
    10141 tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg
    10201 cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga attcagcgag
    10261 agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa
    10321 accgaactgc ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc
    10381 gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact
    10441 acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg
    10501 atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc
    10561 gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg
    10621 acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc
    10681 caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag
    10741 acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat
    10801 atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat
    10861 gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg
    10921 cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc
    10981 gccgatagtg gaaaccgacg ccccagcact cgtccggatc gggagatggg ggaggctaac
    11041 tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca
    11101 gaataaaacg cacgggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct
    11161 ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct
    11221 tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg
    11281 cggcaggccc tgccatagcc actggccccg tgggttaggg acggggtccc ccatggggaa
    11341 tggtttatgg ttcgtggggg ttattatttt gggcgttgcg tggggtcagg tccacgactg
    11401 gactgagcag acagacccat ggtttttgga tggcctgggc atggaccgca tgtactggcg
    11461 cgacacgaac accgggcgtc tgtggctgcc aaacaccccc gacccccaaa aaccaccgcg
    11521 cggatttctg gcgtgccaag ctagtcgacc aattctcatg tttgacagct tatcatcgca
    11581 gatccgggca acgttgttgc cattgctgca ggcgcagaac tggtaggtat ggaagatcta
    11641 tacattgaat caatattggc aattagccat attagtcatt ggttatatag cataaatcaa
    11701 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
    11761 ctcatgtcca atatgaccgc catgttgaca ttgattattg actagttatt aatagtaatc
    11821 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
    11881 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
    11941 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
    12001 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtccgc cccctattga
    12061 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tacgggactt
    12121 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
    12181 gcagtacacc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
    12241 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
    12301 taataacccc gccccgttga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
    12361 aagcagagct cgtttagtga accgtcagat ctctagaagc tgggtaccag ctgctagc
//
