LOCUS       sequence-235160-       11797 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the 25711 HIV-1 isolate; 
            C-terminal truncation; QES mutant with enhanced binding to PG16.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11797)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 11797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-25711-QES.c11-754*
FEATURES             Location/Qualifiers
     source          1..11797
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    2267..2401
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2291..2310)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2345..2364
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2544..2566)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      3126..4915
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(6956..6974)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7042..7146
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7147..7215,7216..8007)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7365..7384)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8178..8766
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8667..8686
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        9197..9342
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             9385..10422
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(10420..10439)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10464..10512
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        11166..11545
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11546..11749
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11700..11720
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     11746..11770
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gccaccatgc ctatggggag cctccaaccc ctggctacac tctatttgct tggtatgctc
       61 gtagccagtg tcttggctaa tctctgggtc acagtatatt atggtgtacc cgtatggaag
      121 gaagctaaga ccaccctctt ctgtgcgtct gacgcgaagg gctacgacaa ggaagttcac
      181 aatgtttggg ctactcacgc atgtgttccg actgatccca acccgcaaga gatgcctctg
      241 gagaatgtta ccgagaattt caatatgtgg gaaaatgaca tggtgaatca aatgcacgaa
      301 gatgtgatca gtctgtggga tgagtccttg aagccttgcg tcaaactgac ccctctgtgc
      361 gtaactttga actgcacgga tgttaataaa aatgtttcct cttccgacac ggataactac
      421 aaagagacta tgaaggagcg gaaaaattgc acgttcaata tgacgacaga gcttcgcgac
      481 aagaaccaga agaaatatgc ccttttctat aagctcgacc tggtcccact ggatgataat
      541 gacaatgcgt cataccggct tataaattgc aatacgagca cgctgactca ggcctgccca
      601 aagatgagtt ttgaccctat accgatccac tactgtgccc ctgcagggta cgcaattctc
      661 aaatgcaaga ataaaacctt caatgggata gggccgtgta acaaggtttc aaccgttcaa
      721 tgcacgcatg gaataaagcc gaccgtatct acacaacttt tgcttaacgg gagtcttgcg
      781 gaagaggaca ttgttattcg ctcagagaac ataaccgata atgcgaaaac aataatcgta
      841 cacctcaatg agtccgtgga gatcgtttgc atccgcccta ataataacac tcggaaatct
      901 attaggatag gtccaggcca aaccttttac gcgacgggtg atatagttgg ggacatccgc
      961 caggcatact gcaatatctc agaggggaaa tggaataaga ctctccagcg ggtatctgag
     1021 aagctggctg agcacttccc gaacagtaca attaacttca atagctcaag cgggggtgac
     1081 ctggagatta cgacgcattc tttcaattgt ggcggggaat ggttctattg caatacgtct
     1141 ggcctcttca acggaaccta tatgaacaat gatacgaaga gcaatgacac aaagtctaac
     1201 agttcatcaa tcatcacgat cccatgtcgc attaagcaaa ttatcaacat gtggcaagaa
     1261 gtcggacgcg cggtatacgc accacccatc gccggtaaca ttacttgtaa atccaacatt
     1321 acgggcattc ttctgactag ggatggtggg agaggcgaag aagtgaaaaa tgataccgag
     1381 acatttaggc caggaggggg caacatgaaa gacaattgga ggagtgagct ttataaatac
     1441 aaagtagttg aaatcaagcc cctcggagtg gcaccaaccg ccgccaagcg cagagtcgtc
     1501 gagagagaaa agagggcagt aggacttggg gcggtgctcc ttggatttct tggggcagcc
     1561 gggagtacga tgggcgcagc ttccatcact ttgacggttc aggcgcgaca actcctctct
     1621 ggaattgttc aacagcagtc taacttgctc cgggccattg aggcgcagca gcacatgttg
     1681 cagctcaccg tgtggggtat taagcagctc caagctaggg tacttgccat cgagcggtat
     1741 cttaaggatc aacagctcct cggaatttgg gggtgttccg gtaagcttat ttgcacaaca
     1801 gcggtcccct ggaactccag ttggtctaat aagaatcaaa ctgagatatg ggacaagatg
     1861 acttggatgc aatgggatag agagatttca aattatacgg acactatata ccgccttctt
     1921 gaagatagcc aaaaccaaca ggaaaagaat gagaaggacc ttttggaact tgacaagtgg
     1981 cagaaccttt ggagttggtt caacataact aactggctct ggtacataag aatattcata
     2041 atgattgtag ggggcttgat aggcttgcga ataatattcg cggttctgtc aatcgtaaac
     2101 cgcgtacgac aaggatattc accgttgtct tttcagaccc tcgcgccgaa tcctcgggga
     2161 ttggaccgcc tggggcggat tgaagaagag ggagggaaag aggacaggaa cagatccatt
     2221 cgacttgtcc acggttttct ttgataactc gaggccggca aggccggatc cagacatgat
     2281 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     2341 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     2401 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
     2461 ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatccgg ctgcctcgcg
     2521 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     2581 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     2641 gggtgtcggg gcgcagccat gaggtcgact ctagaggatc gatccccgcc ccggacgaac
     2701 taaacctgac tacgacatct ctgccccttc ttcgcggggc agtgcatgta atcccttcag
     2761 ttggttggta caacttgcca actgggccct gttccacatg tgacacgggg ggggaccaaa
     2821 cacaaagggg ttctctgact gtagttgaca tccttataaa tggatgtgca catttgccaa
     2881 cactgagtgg ctttcatcct ggagcagact ttgcagtctg tggactgcaa cacaacattg
     2941 cctttatgtg taactcttgg ctgaagctct tacaccaatg ctgggggaca tgtacctccc
     3001 aggggcccag gaagactacg ggaggctaca ccaacgtcaa tcagaggggc ctgtgtagct
     3061 accgataagc ggaccctcaa gagggcatta gcaatagtgt ttataaggcc cccttgttaa
     3121 ccctaaacgg gtagcatatg cttcccgggt agtagtatat actatccaga ctaaccctaa
     3181 ttcaatagca tatgttaccc aacgggaagc atatgctatc gaattagggt tagtaaaagg
     3241 gtcctaagga acagcgatat ctcccacccc atgagctgtc acggttttat ttacatgggg
     3301 tcaggattcc acgagggtag tgaaccattt tagtcacaag ggcagtggct gaagatcaag
     3361 gagcgggcag tgaactctcc tgaatcttcg cctgcttctt cattctcctt cgtttagcta
     3421 atagaataac tgctgagttg tgaacagtaa ggtgtatgtg aggtgctcga aaacaaggtt
     3481 tcaggtgacg cccccagaat aaaatttgga cggggggttc agtggtggca ttgtgctatg
     3541 acaccaatat aaccctcaca aaccccttgg gcaataaata ctagtgtagg aatgaaacat
     3601 tctgaatatc tttaacaata gaaatccatg gggtggggac aagccgtaaa gactggatgt
     3661 ccatctcaca cgaatttatg gctatgggca acacataatc ctagtgcaat atgatactgg
     3721 ggttattaag atgtgtccca ggcagggacc aagacaggtg aaccatgttg ttacactcta
     3781 tttgtaacaa ggggaaagag agtggacgcc gacagcagcg gactccactg gttgtctcta
     3841 acacccccga aaattaaacg gggctccacg ccaatggggc ccataaacaa agacaagtgg
     3901 ccactctttt ttttgaaatt gtggagtggg ggcacgcgtc agcccccaca cgccgccctg
     3961 cggttttgga ctgtaaaata agggtgtaat aacttggctg attgtaaccc cgctaaccac
     4021 tgcggtcaaa ccacttgccc acaaaaccac taatggcacc ccggggaata cctgcataag
     4081 taggtgggcg ggccaagata ggggcgcgat tgctgcgatc tggaggacaa attacacaca
     4141 cttgcgcctg agcgccaagc acagggttgt tggtcctcat attcacgagg tcgctgagag
     4201 cacggtgggc taatgttgcc atgggtagca tatactaccc aaatatctgg atagcatatg
     4261 ctatcctaat ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg
     4321 ctatcctaat ctatatctgg gtagtatatg ctatcctaat ttatatctgg gtagcatagg
     4381 ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg
     4441 ctatcctaat ctgtatccgg gtagcatatg ctatcctaat agagattagg gtagtatatg
     4501 ctatcctaat ttatatctgg gtagcatata ctacccaaat atctggatag catatgctat
     4561 cctaatctat atctgggtag catatgctat cctaatctat atctgggtag cataggctat
     4621 cctaatctat atctgggtag catatgctat cctaatctat atctgggtag tatatgctat
     4681 cctaatttat atctgggtag cataggctat cctaatctat atctgggtag catatgctat
     4741 cctaatctat atctgggtag tatatgctat cctaatctgt atccgggtag catatgctat
     4801 cctcatgcat atacagtcag catatgatac ccagtagtag agtgggagtg ctatcctttg
     4861 catatgccgc cacctcccaa gggggcgtga attttcgctg cttgtccttt tcctgctggt
     4921 tgctcccatt cttaggtgaa tttaaggagg ccaggctaaa gccgtcgcat gtctgattgc
     4981 tcaccaggta aatgtcgcta atgttttcca acgcgagaag gtgttgagcg cggagctgag
     5041 tgacgtgaca acatgggtat gcccaattgc cccatgttgg gaggacgaaa atggtgacaa
     5101 gacagatggc cagaaataca ccaacagcac gcatgatgtc tactggggat ttattcttta
     5161 gtgcggggga atacacggct tttaatacga ttgagggcgt ctcctaacaa gttacatcac
     5221 tcctgccctt cctcaccctc atctccatca cctccttcat ctccgtcatc tccgtcatca
     5281 ccctccgcgg cagccccttc caccataggt ggaaaccagg gaggcaaatc tactccatcg
     5341 tcaaagctgc acacagtcac cctgatattg caggtaggag cgggctttgt cataacaagg
     5401 tccttaatcg catccttcaa aacctcagca aatatatgag tttgtaaaaa gaccatgaaa
     5461 taacagacaa tggactccct tagcgggcca ggttgtgggc cgggtccagg ggccattcca
     5521 aaggggagac gactcaatgg tgtaagacga cattgtggaa tagcaagggc agttcctcgc
     5581 cttaggttgt aaagggaggt cttactacct ccatatacga acacaccggc gacccaagtt
     5641 ccttcgtcgg tagtcctttc tacgtgactc ctagccagga gagctcttaa accttctgca
     5701 atgttctcaa atttcgggtt ggaacctcct tgaccacgat gctttccaaa ccaccctcct
     5761 tttttgcgcc tgcctccatc accctgaccc cggggtccag tgcttgggcc ttctcctggg
     5821 tcatctgcgg ggccctgctc tatcgctccc gggggcacgt caggctcacc atctgggcca
     5881 ccttcttggt ggtattcaaa ataatcggct tcccctacag ggtggaaaaa tggccttcta
     5941 cctggagggg gcctgcgcgg tggagacccg gatgatgatg actgactact gggactcctg
     6001 ggcctctttt ctccacgtcc acgacctctc cccctggctc tttcacgact tccccccctg
     6061 gctctttcac gtcctctacc ccggcggcct ccactacctc ctcgaccccg gcctccacta
     6121 cctcctcgac cccggcctcc actgcctcct cgaccccggc ctccacctcc tgctcctgcc
     6181 cctcctcctg ctcctgcccc tcctcctgct cctgcccctc ctgctcctgc ccctcccgct
     6241 cctgctcctg ctcctgttcc accgtgggtc cctttgcagc caatgcaact tggacgtttt
     6301 tggggtctcc ggacaccatc tctatgtctt ggccctgatc ctgagccgcc cggggctcct
     6361 ggtcttccgc ctcctcgtcc tcgtcctctt ccccgtcctc gtccatggtt atcaccccct
     6421 cttctttgag gtccactgcc gccggagcct tctggtccag atgtgtctcc cttctctcct
     6481 aggccatttc caggtcctgt acctggcccc tcgtcagaca tgattcacac taaaagagat
     6541 caatagacat ctttattaga cgacgctcag tgaatacagg gagtgcagac tcctgccccc
     6601 tccaacagcc cccccaccct catccccttc atggtcgctg tcagacagat ccaggtctga
     6661 aaattcccca tcctccgaac catcctcgtc ctcatcacca attactcgca gcccggaaaa
     6721 ctcccgctga acatcctcaa gatttgcgtc ctgagcctca agccaggcct caaattcctc
     6781 gtcccccttt ttgctggacg gtagggatgg ggattctcgg gacccctcct cttcctcttc
     6841 aaggtcacca gacagagatg ctactggggc aacggaagaa aagctgggtg cggcctgtga
     6901 ggatcagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc
     6961 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     7021 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     7081 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     7141 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     7201 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     7261 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     7321 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     7381 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     7441 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     7501 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     7561 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     7621 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     7681 acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     7741 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     7801 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     7861 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     7921 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     7981 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     8041 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     8101 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     8161 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     8221 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     8281 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     8341 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     8401 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     8461 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     8521 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     8581 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     8641 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     8701 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     8761 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttgaagc
     8821 tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg
     8881 atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac
     8941 gccagcaaga cgtagcccag cgcgtcggcc ccgagatgcg ccgcgtgcgg ctgctggaga
     9001 tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca
     9061 agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccctgctt
     9121 catccccgtg gcccgttgct cgcgtttgct ggcggtgtcc ccggaagaaa tatatttgca
     9181 tgtctttagt tctatgatga cacaaacccc gcccagcgtc ttgtcattgg cgaattcgaa
     9241 cacgcagatg cagtcggggc ggcgcggtcc gaggtccact tcgcatatta aggtgacgcg
     9301 tgtggcctcg aacaccgagc gaccctgcag cgacccgctt aacagcgtca acagcgtgcc
     9361 gcagatcccg gggggcaatg agatatgaaa aagcctgaac tcaccgcgac gtctgtcgag
     9421 aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa
     9481 gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc
     9541 tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc
     9601 ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta ttgcatctcc
     9661 cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg
     9721 cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg
     9781 ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc
     9841 gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg
     9901 tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg
     9961 cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca
    10021 gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc
    10081 ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg
    10141 catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac
    10201 caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga
    10261 tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga
    10321 agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc
    10381 cccagcactc gtccggatcg ggagatgggg gaggctaact gaaacacgga aggagacaat
    10441 accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc acgggtgttg
    10501 ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac
    10561 cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa
    10621 gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcca
    10681 ctggccccgt gggttaggga cggggtcccc catggggaat ggtttatggt tcgtgggggt
    10741 tattattttg ggcgttgcgt ggggtcaggt ccacgactgg actgagcaga cagacccatg
    10801 gtttttggat ggcctgggca tggaccgcat gtactggcgc gacacgaaca ccgggcgtct
    10861 gtggctgcca aacacccccg acccccaaaa accaccgcgc ggatttctgg cgtgccaagc
    10921 tagtcgacca attctcatgt ttgacagctt atcatcgcag atccgggcaa cgttgttgcc
    10981 attgctgcag gcgcagaact ggtaggtatg gaagatctat acattgaatc aatattggca
    11041 attagccata ttagtcattg gttatatagc ataaatcaat attggctatt ggccattgca
    11101 tacgttgtat ctatatcata atatgtacat ttatattggc tcatgtccaa tatgaccgcc
    11161 atgttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
    11221 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
    11281 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
    11341 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
    11401 acatcaagtg tatcatatgc caagtccgcc ccctattgac gtcaatgacg gtaaatggcc
    11461 cgcctggcat tatgcccagt acatgacctt acgggacttt cctacttggc agtacatcta
    11521 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacacca atgggcgtgg
    11581 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
    11641 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aataaccccg ccccgttgac
    11701 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa
    11761 ccgtcagatc tctagaagct gggtaccagc tgctagc
//
