LOCUS       sequence-235162-       12667 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the 25711 HIV-1 isolate; 
            QES mutant with enhanced binding to PG16.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12667)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 12667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-25711-QES.c11
FEATURES             Location/Qualifiers
     source          1..12667
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    2576..2710
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2600..2619)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2654..2673
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2853..2875)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(5466..7391)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(7826..7844)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7912..8016
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8017..8085,8086..8877)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8235..8254)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9048..9636
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9537..9556
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        10067..10212
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             10255..11292
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(11290..11309)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11334..11382
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        12036..12415
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12416..12619
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     12570..12590
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     12616..12640
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gccaccatgc ctatggggag cctccaaccc ctggctacac tctatttgct tggtatgctc
       61 gtagccagtg tcttggctaa tctctgggtc acagtatatt atggtgtacc cgtatggaag
      121 gaagctaaga ccaccctctt ctgtgcgtct gacgcgaagg gctacgacaa ggaagttcac
      181 aatgtttggg ctactcacgc atgtgttccg actgatccca acccgcaaga gatgcctctg
      241 gagaatgtta ccgagaattt caatatgtgg gaaaatgaca tggtgaatca aatgcacgaa
      301 gatgtgatca gtctgtggga tgagtccttg aagccttgcg tcaaactgac ccctctgtgc
      361 gtaactttga actgcacgga tgttaataaa aatgtttcct cttccgacac ggataactac
      421 aaagagacta tgaaggagcg gaaaaattgc acgttcaata tgacgacaga gcttcgcgac
      481 aagaaccaga agaaatatgc ccttttctat aagctcgacc tggtcccact ggatgataat
      541 gacaatgcgt cataccggct tataaattgc aatacgagca cgctgactca ggcctgccca
      601 aagatgagtt ttgaccctat accgatccac tactgtgccc ctgcagggta cgcaattctc
      661 aaatgcaaga ataaaacctt caatgggata gggccgtgta acaaggtttc aaccgttcaa
      721 tgcacgcatg gaataaagcc gaccgtatct acacaacttt tgcttaacgg gagtcttgcg
      781 gaagaggaca ttgttattcg ctcagagaac ataaccgata atgcgaaaac aataatcgta
      841 cacctcaatg agtccgtgga gatcgtttgc atccgcccta ataataacac tcggaaatct
      901 attaggatag gtccaggcca aaccttttac gcgacgggtg atatagttgg ggacatccgc
      961 caggcatact gcaatatctc agaggggaaa tggaataaga ctctccagcg ggtatctgag
     1021 aagctggctg agcacttccc gaacagtaca attaacttca atagctcaag cgggggtgac
     1081 ctggagatta cgacgcattc tttcaattgt ggcggggaat ggttctattg caatacgtct
     1141 ggcctcttca acggaaccta tatgaacaat gatacgaaga gcaatgacac aaagtctaac
     1201 agttcatcaa tcatcacgat cccatgtcgc attaagcaaa ttatcaacat gtggcaagaa
     1261 gtcggacgcg cggtatacgc accacccatc gccggtaaca ttacttgtaa atccaacatt
     1321 acgggcattc ttctgactag ggatggtggg agaggcgaag aagtgaaaaa tgataccgag
     1381 acatttaggc caggaggggg caacatgaaa gacaattgga ggagtgagct ttataaatac
     1441 aaagtagttg aaatcaagcc cctcggagtg gcaccaaccg ccgccaagcg cagagtcgtc
     1501 gagagagaaa agagggcagt aggacttggg gcggtgctcc ttggatttct tggggcagcc
     1561 gggagtacga tgggcgcagc ttccatcact ttgacggttc aggcgcgaca actcctctct
     1621 ggaattgttc aacagcagtc taacttgctc cgggccattg aggcgcagca gcacatgttg
     1681 cagctcaccg tgtggggtat taagcagctc caagctaggg tacttgccat cgagcggtat
     1741 cttaaggatc aacagctcct cggaatttgg gggtgttccg gtaagcttat ttgcacaaca
     1801 gcggtcccct ggaactccag ttggtctaat aagaatcaaa ctgagatatg ggacaagatg
     1861 acttggatgc aatgggatag agagatttca aattatacgg acactatata ccgccttctt
     1921 gaagatagcc aaaaccaaca ggaaaagaat gagaaggacc ttttggaact tgacaagtgg
     1981 cagaaccttt ggagttggtt caacataact aactggctct ggtacataag aatattcata
     2041 atgattgtag ggggcttgat aggcttgcga ataatattcg cggttctgtc aatcgtaaac
     2101 cgcgtacgac aaggatattc accgttgtct tttcagaccc tcgcgccgaa tcctcgggga
     2161 ttggaccgcc tggggcggat tgaagaagag ggagggaaag aggacaggaa cagatccatt
     2221 cgacttgtcc acggttttct tgcgcttgcg tgggacgatt tgcggtcact ctgtctgttt
     2281 agctaccacc ggctcagaga ccttatcttg ctcattgcgc gcgctgttga attgctgggt
     2341 caacgcgggt gggaagcgct taaatatctc gcggggatag ttcaatattg gggtcttgaa
     2401 ttgaaaaaat ccgcagtatc actgttcgat accatagcga ttgccgtcgc agagagaact
     2461 gaccgaataa tagggttgat acagggcatc tgccgcgcaa tctgcaatat acccaggcgg
     2521 attcgacagg gcttcgaggc agctctccag tagtaactcg aggccggcaa ggccggatcc
     2581 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     2641 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     2701 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
     2761 ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatccggc
     2821 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     2881 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     2941 ggtgttggcg ggtgtcgggg cgcagccatg aggtcgactc tagaggatcg atccccgccc
     3001 cggacgaact aaacctgact acgacatctc tgccccttct tcgcggggca gtgcatgtaa
     3061 tcccttcagt tggttggtac aacttgccaa ctgggccctg ttccacatgt gacacggggg
     3121 gggaccaaac acaaaggggt tctctgactg tagttgacat ccttataaat ggatgtgcac
     3181 atttgccaac actgagtggc tttcatcctg gagcagactt tgcagtctgt ggactgcaac
     3241 acaacattgc ctttatgtgt aactcttggc tgaagctctt acaccaatgc tgggggacat
     3301 gtacctccca ggggcccagg aagactacgg gaggctacac caacgtcaat cagaggggcc
     3361 tgtgtagcta ccgataagcg gaccctcaag agggcattag caatagtgtt tataaggccc
     3421 ccttgttaac cctaaacggg tagcatatgc ttcccgggta gtagtatata ctatccagac
     3481 taaccctaat tcaatagcat atgttaccca acgggaagca tatgctatcg aattagggtt
     3541 agtaaaaggg tcctaaggaa cagcgatatc tcccacccca tgagctgtca cggttttatt
     3601 tacatggggt caggattcca cgagggtagt gaaccatttt agtcacaagg gcagtggctg
     3661 aagatcaagg agcgggcagt gaactctcct gaatcttcgc ctgcttcttc attctccttc
     3721 gtttagctaa tagaataact gctgagttgt gaacagtaag gtgtatgtga ggtgctcgaa
     3781 aacaaggttt caggtgacgc ccccagaata aaatttggac ggggggttca gtggtggcat
     3841 tgtgctatga caccaatata accctcacaa accccttggg caataaatac tagtgtagga
     3901 atgaaacatt ctgaatatct ttaacaatag aaatccatgg ggtggggaca agccgtaaag
     3961 actggatgtc catctcacac gaatttatgg ctatgggcaa cacataatcc tagtgcaata
     4021 tgatactggg gttattaaga tgtgtcccag gcagggacca agacaggtga accatgttgt
     4081 tacactctat ttgtaacaag gggaaagaga gtggacgccg acagcagcgg actccactgg
     4141 ttgtctctaa cacccccgaa aattaaacgg ggctccacgc caatggggcc cataaacaaa
     4201 gacaagtggc cactcttttt tttgaaattg tggagtgggg gcacgcgtca gcccccacac
     4261 gccgccctgc ggttttggac tgtaaaataa gggtgtaata acttggctga ttgtaacccc
     4321 gctaaccact gcggtcaaac cacttgccca caaaaccact aatggcaccc cggggaatac
     4381 ctgcataagt aggtgggcgg gccaagatag gggcgcgatt gctgcgatct ggaggacaaa
     4441 ttacacacac ttgcgcctga gcgccaagca cagggttgtt ggtcctcata ttcacgaggt
     4501 cgctgagagc acggtgggct aatgttgcca tgggtagcat atactaccca aatatctgga
     4561 tagcatatgc tatcctaatc tatatctggg tagcataggc tatcctaatc tatatctggg
     4621 tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatt tatatctggg
     4681 tagcataggc tatcctaatc tatatccggg tagcatatgc tatcctaata gagattaggg
     4741 tagtatatgc tatcctaatt tatatctggg tagcatatac tacccaaata tctggatagc
     4801 atatgctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagc
     4861 ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt
     4921 atatgctatc ctaatttata tctgggtagc ataggctatc ctaatctata tctgggtagc
     4981 atatgctatc ctaatctata tctgggtagt atatgctatc ctaatctgta tccgggtagc
     5041 atatgctatc ctcatgcata tacagtcagc atatgatacc cagtagtaga gtgggagtgc
     5101 tatcctttgc atatgccgcc acctcccaag ggggcgtgaa ttttcgctgc ttgtcctttt
     5161 cctgctggtt gctcccattc ttaggtgaat ttaaggaggc caggctaaag ccgtcgcatg
     5221 tctgattgct caccaggtaa atgtcgctaa tgttttccaa cgcgagaagg tgttgagcgc
     5281 ggagctgagt gacgtgacaa catgggtatg cccaattgcc ccatgttggg aggacgaaaa
     5341 tggtgacaag acagatggcc agaaatacac caacagcacg catgatgtct actggggatt
     5401 tattctttag tgcgggggaa tacacggctt ttaatacgat tgagggcgtc tcctaacaag
     5461 ttacatcact cctgcccttc ctcaccctca tctccatcac ctccttcatc tccgtcatct
     5521 ccgtcatcac cctccgcggc agccccttcc accataggtg gaaaccaggg aggcaaatct
     5581 actccatcgt caaagctgca cacagtcacc ctgatattgc aggtaggagc gggctttgtc
     5641 ataacaaggt ccttaatcgc atccttcaaa acctcagcaa atatatgagt ttgtaaaaag
     5701 accatgaaat aacagacaat ggactccctt agcgggccag gttgtgggcc gggtccaggg
     5761 gccattccaa aggggagacg actcaatggt gtaagacgac attgtggaat agcaagggca
     5821 gttcctcgcc ttaggttgta aagggaggtc ttactacctc catatacgaa cacaccggcg
     5881 acccaagttc cttcgtcggt agtcctttct acgtgactcc tagccaggag agctcttaaa
     5941 ccttctgcaa tgttctcaaa tttcgggttg gaacctcctt gaccacgatg ctttccaaac
     6001 caccctcctt ttttgcgcct gcctccatca ccctgacccc ggggtccagt gcttgggcct
     6061 tctcctgggt catctgcggg gccctgctct atcgctcccg ggggcacgtc aggctcacca
     6121 tctgggccac cttcttggtg gtattcaaaa taatcggctt cccctacagg gtggaaaaat
     6181 ggccttctac ctggaggggg cctgcgcggt ggagacccgg atgatgatga ctgactactg
     6241 ggactcctgg gcctcttttc tccacgtcca cgacctctcc ccctggctct ttcacgactt
     6301 ccccccctgg ctctttcacg tcctctaccc cggcggcctc cactacctcc tcgaccccgg
     6361 cctccactac ctcctcgacc ccggcctcca ctgcctcctc gaccccggcc tccacctcct
     6421 gctcctgccc ctcctgctcc tgcccctcct cctgctcctg cccctcctgc ccctcctgct
     6481 cctgcccctc ctgcccctcc tgctcctgcc cctcctgccc ctcctgctcc tgcccctcct
     6541 gcccctcctc ctgctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctgcccct
     6601 cctgctcctg cccctcctgc ccctcctgct cctgcccctc ctgcccctcc tgctcctgcc
     6661 cctcctgctc ctgcccctcc tgctcctgcc cctcctgctc ctgcccctcc tgcccctcct
     6721 gcccctcctc ctgctcctgc ccctcctgct cctgcccctc ctgcccctcc tgcccctcct
     6781 gctcctgccc ctcctcctgc tcctgcccct cctgcccctc ctgcccctcc tcctgctcct
     6841 gcccctcctg cccctcctcc tgctcctgcc cctcctcctg ctcctgcccc tcctgcccct
     6901 cctgcccctc ctcctgctcc tgcccctcct gcccctcctc ctgctcctgc ccctcctcct
     6961 gctcctgccc ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tcctgctcct
     7021 gcccctcctg cccctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctcctgct
     7081 cctgcccctc ctgctcctgc ccctcccgct cctgctcctg ctcctgttcc accgtgggtc
     7141 cctttgcagc caatgcaact tggacgtttt tggggtctcc ggacaccatc tctatgtctt
     7201 ggccctgatc ctgagccgcc cggggctcct ggtcttccgc ctcctcgtcc tcgtcctctt
     7261 ccccgtcctc gtccatggtt atcaccccct cttctttgag gtccactgcc gccggagcct
     7321 tctggtccag atgtgtctcc cttctctcct aggccatttc caggtcctgt acctggcccc
     7381 tcgtcagaca tgattcacac taaaagagat caatagacat ctttattaga cgacgctcag
     7441 tgaatacagg gagtgcagac tcctgccccc tccaacagcc cccccaccct catccccttc
     7501 atggtcgctg tcagacagat ccaggtctga aaattcccca tcctccgaac catcctcgtc
     7561 ctcatcacca attactcgca gcccggaaaa ctcccgctga acatcctcaa gatttgcgtc
     7621 ctgagcctca agccaggcct caaattcctc gtcccccttt ttgctggacg gtagggatgg
     7681 ggattctcgg gacccctcct cttcctcttc aaggtcacca gacagagatg ctactggggc
     7741 aacggaagaa aagctgggtg cggcctgtga ggatcagctt atcgatgata agctgtcaaa
     7801 catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     7861 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     7921 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     7981 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     8041 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     8101 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     8161 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     8221 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa
     8281 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     8341 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     8401 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     8461 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     8521 gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg
     8581 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     8641 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     8701 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     8761 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     8821 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     8881 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     8941 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     9001 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     9061 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     9121 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     9181 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     9241 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     9301 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     9361 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     9421 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     9481 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     9541 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     9601 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     9661 cggttcctgg ccttttgctg gccttgaagc tgtccctgat ggtcgtcatc tacctgcctg
     9721 gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg
     9781 gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc
     9841 ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag
     9901 ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg
     9961 tgaatccgtt agcgaggtgc cgccctgctt catccccgtg gcccgttgct cgcgtttgct
    10021 ggcggtgtcc ccggaagaaa tatatttgca tgtctttagt tctatgatga cacaaacccc
    10081 gcccagcgtc ttgtcattgg cgaattcgaa cacgcagatg cagtcggggc ggcgcggtcc
    10141 gaggtccact tcgcatatta aggtgacgcg tgtggcctcg aacaccgagc gaccctgcag
    10201 cgacccgctt aacagcgtca acagcgtgcc gcagatcccg gggggcaatg agatatgaaa
    10261 aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc
    10321 tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga
    10381 gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat
    10441 gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggaa
    10501 ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac
    10561 ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat ggatgcgatc
    10621 gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt
    10681 caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg
    10741 caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg
    10801 ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt cggctccaac
    10861 aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga ggcgatgttc
    10921 ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg
    10981 gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc gccgcggctc
    11041 cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt tgacggcaat
    11101 ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg
    11161 actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga tggctgtgta
    11221 gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccggatcg ggagatgggg
    11281 gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat
    11341 aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt tcataaacgc ggggttcggt
    11401 cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg
    11461 tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca
    11521 acgtcggggc ggcaggccct gccatagcca ctggccccgt gggttaggga cggggtcccc
    11581 catggggaat ggtttatggt tcgtgggggt tattattttg ggcgttgcgt ggggtcaggt
    11641 ccacgactgg actgagcaga cagacccatg gtttttggat ggcctgggca tggaccgcat
    11701 gtactggcgc gacacgaaca ccgggcgtct gtggctgcca aacacccccg acccccaaaa
    11761 accaccgcgc ggatttctgg cgtgccaagc tagtcgacca attctcatgt ttgacagctt
    11821 atcatcgcag atccgggcaa cgttgttgcc attgctgcag gcgcagaact ggtaggtatg
    11881 gaagatctat acattgaatc aatattggca attagccata ttagtcattg gttatatagc
    11941 ataaatcaat attggctatt ggccattgca tacgttgtat ctatatcata atatgtacat
    12001 ttatattggc tcatgtccaa tatgaccgcc atgttgacat tgattattga ctagttatta
    12061 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
    12121 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
    12181 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
    12241 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtccgcc
    12301 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
    12361 acgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
    12421 gcggttttgg cagtacacca atgggcgtgg atagcggttt gactcacggg gatttccaag
    12481 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
    12541 aaaatgtcgt aataaccccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga
    12601 ggtctatata agcagagctc gtttagtgaa ccgtcagatc tctagaagct gggtaccagc
    12661 tgctagc
//
