LOCUS       sequence-235165-       12469 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the ZM197M HIV-1 isolate; 
            C-terminal truncation; QES mutant with enhanced binding to PG16.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12469)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 12469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-ZM197M-QES.c10-754*
FEATURES             Location/Qualifiers
     source          1..12469
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    2318..2452
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2342..2361)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2396..2415
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2595..2617)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      3177..4966
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(5268..7193)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(7628..7646)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7714..7818
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7819..7887,7888..8679)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8037..8056)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8850..9438
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9339..9358
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        9869..10014
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             10057..11094
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(11092..11111)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11136..11184
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        11838..12217
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12218..12421
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     12372..12392
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     12418..12442
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gccaccatgc ccatggggag tctccaaccg ctggcaacgc tgtatcttct cggtatgttg
       61 gtggcgtcag tcttggcgga acaactgtgg gttacggtgt attacggtgt tccggtgtgg
      121 aaagaggcta aagcaactct cttctgcgcc agcgatgcga aagcctacga aaaagaagta
      181 cataacgtgt gggccaccca tgcttgcgta cccacagatc cgaatcccca agagataccc
      241 ttgggtaacg tgacggaaaa tttcaatatg tggaagaacg acatggcaga ccaaatgcac
      301 gaagacataa ttagtttgtg ggaccaaagc ttgaaaccat gcgtcaaact gaccccattg
      361 tgcgtgactc tgaattgcag cgacgctaca agtaatacga caaagaatgc gactaacaca
      421 aacacgactt caacagacaa ccgaaatgcg acaagtaacg ataccgaaat gaaaggggaa
      481 attaaagatt gtacctttaa tatcacaact gaagtccgag acagaaagac taagcaacgc
      541 gcactgttct ataaacttga tgtggtccct ctggaggagg agaaaaactc cagctctaaa
      601 aacagctctt acaaagagta ccgattgatt agctgcaaca cgagcacaat tacccaagcc
      661 tgtccaaaaa tgtcttttga cccaattccg atccactact gcgctccagc aggctatgcg
      721 atacttaaat gtaacaataa aacattcaac ggtaccggac catgtcataa cgtcagtacg
      781 gttcagtgca cgcacgggat aaaaccaact atgtctacac aactgcttct taacggttct
      841 cttgccgagg aggaaatcat catacggtct gagaacctga ctgacaacac taaaacaatt
      901 atagtgcatt tgaacgagtc agtcgagatt gaatgcgtta ggcccaataa taacacgagg
      961 aaaagcgtca gaatcggtcc cgggcaaact ttcttcgcta caggggaaat cataggtgac
     1021 attcgccagg cgcattgcga tctttcaaag tctaattgga ccacgacgct taaaaggatt
     1081 gagaagaaat tgaaggaaca cttcaataac gctaccatca agtttgaatc ttccgctggc
     1141 ggcgaccttg agatcactac ccattctttt aattgtcgcg gcgagttctt ctattgtaat
     1201 acgtctggcc tcttcaactc ttctctgctg aacgatacag acggaacgag caactctact
     1261 tctaatgcaa ccatcacgct cccgtgccgc ataaaacaaa tcatcaatat gtggcaggaa
     1321 gtggggcggg caatgtacgc ctcaccgatt gccgggatca taacatgcaa aagtaacatc
     1381 accgggctct tgctcacacg agacggtggg aataaaagcg cagggataga gacctttcgc
     1441 ccagggggcg ggaacatgaa agataattgg cgatccgagc tgtataaata caaggtggtt
     1501 gaaatcaaac cccttgggat tgcacctact agcgcgaaac ggcgggtcgt cgaaagagag
     1561 aaaagagccg cggggatcgg cgcggtaata ttgggctttc tcggcgcagc gggttcaact
     1621 atgggcgctg ctagtgtgat gcttactgtc caggcacgac agttgttgag tggtattgtt
     1681 cagcaacaat ctaacctgct tcgcgctatc gaggcacaac agcatatgtt gcaacttaca
     1741 gtatggggaa ttaaacaact gcaaactcgg gtactggcta tcgagcgcta ccttaaggat
     1801 cagcaattgc ttggtttgtg ggggtgcagt ggtaaactga tctgtaccac agcggtaccc
     1861 tggaacacca gttggtctaa caaaagcaaa gacgaaatat gggacaacat gacatggatg
     1921 cagtgggatc gggaaataga taactacacc caggtcatct accaacttct cgaagtatct
     1981 cagaatcagc aggagaagaa cgagaatgat ctccttgctt tggataagtg gaacagcctg
     2041 tggagttggt ttgatataac gaagtggctc tggtacatta agatatttat catgatcgtt
     2101 gggggtttga ttggtttgag gattattttt gctgtattgt cagtggtcaa tcgggtacgg
     2161 cagggttatt ccccgctctc cttccaaacg ctgattccca atccacgggg acccgacaga
     2221 ccaggaggta ttgaggaaga gggtggcgag caagataaga ataggtcaac gagattggtt
     2281 agcggctttt tgtgataact cgaggccggc aaggccggat ccagacatga taagatacat
     2341 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     2401 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     2461 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa
     2521 gtaaaacctc tacaaatgtg gtatggctga ttatgatccg gctgcctcgc gcgtttcggt
     2581 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     2641 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     2701 ggcgcagcca tgaggtcgac tctagaggat cgatccccgc cccggacgaa ctaaacctga
     2761 ctacgacatc tctgcccctt cttcgcgggg cagtgcatgt aatcccttca gttggttggt
     2821 acaacttgcc aactgggccc tgttccacat gtgacacggg gggggaccaa acacaaaggg
     2881 gttctctgac tgtagttgac atccttataa atggatgtgc acatttgcca acactgagtg
     2941 gctttcatcc tggagcagac tttgcagtct gtggactgca acacaacatt gcctttatgt
     3001 gtaactcttg gctgaagctc ttacaccaat gctgggggac atgtacctcc caggggccca
     3061 ggaagactac gggaggctac accaacgtca atcagagggg cctgtgtagc taccgataag
     3121 cggaccctca agagggcatt agcaatagtg tttataaggc ccccttgtta accctaaacg
     3181 ggtagcatat gcttcccggg tagtagtata tactatccag actaacccta attcaatagc
     3241 atatgttacc caacgggaag catatgctat cgaattaggg ttagtaaaag ggtcctaagg
     3301 aacagcgata tctcccaccc catgagctgt cacggtttta tttacatggg gtcaggattc
     3361 cacgagggta gtgaaccatt ttagtcacaa gggcagtggc tgaagatcaa ggagcgggca
     3421 gtgaactctc ctgaatcttc gcctgcttct tcattctcct tcgtttagct aatagaataa
     3481 ctgctgagtt gtgaacagta aggtgtatgt gaggtgctcg aaaacaaggt ttcaggtgac
     3541 gcccccagaa taaaatttgg acggggggtt cagtggtggc attgtgctat gacaccaata
     3601 taaccctcac aaaccccttg ggcaataaat actagtgtag gaatgaaaca ttctgaatat
     3661 ctttaacaat agaaatccat ggggtgggga caagccgtaa agactggatg tccatctcac
     3721 acgaatttat ggctatgggc aacacataat cctagtgcaa tatgatactg gggttattaa
     3781 gatgtgtccc aggcagggac caagacaggt gaaccatgtt gttacactct atttgtaaca
     3841 aggggaaaga gagtggacgc cgacagcagc ggactccact ggttgtctct aacacccccg
     3901 aaaattaaac ggggctccac gccaatgggg cccataaaca aagacaagtg gccactcttt
     3961 tttttgaaat tgtggagtgg gggcacgcgt cagcccccac acgccgccct gcggttttgg
     4021 actgtaaaat aagggtgtaa taacttggct gattgtaacc ccgctaacca ctgcggtcaa
     4081 accacttgcc cacaaaacca ctaatggcac cccggggaat acctgcataa gtaggtgggc
     4141 gggccaagat aggggcgcga ttgctgcgat ctggaggaca aattacacac acttgcgcct
     4201 gagcgccaag cacagggttg ttggtcctca tattcacgag gtcgctgaga gcacggtggg
     4261 ctaatgttgc catgggtagc atatactacc caaatatctg gatagcatat gctatcctaa
     4321 tctatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa
     4381 tctatatctg ggtagtatat gctatcctaa tttatatctg ggtagcatag gctatcctaa
     4441 tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa
     4501 tctgtatccg ggtagcatat gctatcctaa tagagattag ggtagtatat gctatcctaa
     4561 tttatatctg ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta
     4621 tatctgggta gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta
     4681 tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta
     4741 tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
     4801 tatctgggta gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctcatgca
     4861 tatacagtca gcatatgata cccagtagta gagtgggagt gctatccttt gcatatgccg
     4921 ccacctccca agggggcgtg aattttcgct gcttgtcctt ttcctgctgg ttgctcccat
     4981 tcttaggtga atttaaggag gccaggctaa agccgtcgca tgtctgattg ctcaccaggt
     5041 aaatgtcgct aatgttttcc aacgcgagaa ggtgttgagc gcggagctga gtgacgtgac
     5101 aacatgggta tgcccaattg ccccatgttg ggaggacgaa aatggtgaca agacagatgg
     5161 ccagaaatac accaacagca cgcatgatgt ctactgggga tttattcttt agtgcggggg
     5221 aatacacggc ttttaatacg attgagggcg tctcctaaca agttacatca ctcctgccct
     5281 tcctcaccct catctccatc acctccttca tctccgtcat ctccgtcatc accctccgcg
     5341 gcagcccctt ccaccatagg tggaaaccag ggaggcaaat ctactccatc gtcaaagctg
     5401 cacacagtca ccctgatatt gcaggtagga gcgggctttg tcataacaag gtccttaatc
     5461 gcatccttca aaacctcagc aaatatatga gtttgtaaaa agaccatgaa ataacagaca
     5521 atggactccc ttagcgggcc aggttgtggg ccgggtccag gggccattcc aaaggggaga
     5581 cgactcaatg gtgtaagacg acattgtgga atagcaaggg cagttcctcg ccttaggttg
     5641 taaagggagg tcttactacc tccatatacg aacacaccgg cgacccaagt tccttcgtcg
     5701 gtagtccttt ctacgtgact cctagccagg agagctctta aaccttctgc aatgttctca
     5761 aatttcgggt tggaacctcc ttgaccacga tgctttccaa accaccctcc ttttttgcgc
     5821 ctgcctccat caccctgacc ccggggtcca gtgcttgggc cttctcctgg gtcatctgcg
     5881 gggccctgct ctatcgctcc cgggggcacg tcaggctcac catctgggcc accttcttgg
     5941 tggtattcaa aataatcggc ttcccctaca gggtggaaaa atggccttct acctggaggg
     6001 ggcctgcgcg gtggagaccc ggatgatgat gactgactac tgggactcct gggcctcttt
     6061 tctccacgtc cacgacctct ccccctggct ctttcacgac ttccccccct ggctctttca
     6121 cgtcctctac cccggcggcc tccactacct cctcgacccc ggcctccact acctcctcga
     6181 ccccggcctc cactgcctcc tcgaccccgg cctccacctc ctgctcctgc ccctcctgct
     6241 cctgcccctc ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc tcctgcccct
     6301 cctgctcctg cccctcctgc ccctcctgct cctgcccctc ctgcccctcc tcctgctcct
     6361 gcccctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctgctcc tgcccctcct
     6421 gcccctcctg ctcctgcccc tcctgcccct cctgctcctg cccctcctgc tcctgcccct
     6481 cctgctcctg cccctcctgc tcctgcccct cctgcccctc ctgcccctcc tcctgctcct
     6541 gcccctcctg ctcctgcccc tcctgcccct cctgcccctc ctgctcctgc ccctcctcct
     6601 gctcctgccc ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tgcccctcct
     6661 cctgctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctgcccc tcctcctgct
     6721 cctgcccctc ctgcccctcc tcctgctcct gcccctcctc ctgctcctgc ccctcctgcc
     6781 cctcctgccc ctcctcctgc tcctgcccct cctcctgctc ctgcccctcc tgcccctcct
     6841 gcccctcctg cccctcctcc tgctcctgcc cctcctcctg ctcctgcccc tcctgctcct
     6901 gcccctcccg ctcctgctcc tgctcctgtt ccaccgtggg tccctttgca gccaatgcaa
     6961 cttggacgtt tttggggtct ccggacacca tctctatgtc ttggccctga tcctgagccg
     7021 cccggggctc ctggtcttcc gcctcctcgt cctcgtcctc ttccccgtcc tcgtccatgg
     7081 ttatcacccc ctcttctttg aggtccactg ccgccggagc cttctggtcc agatgtgtct
     7141 cccttctctc ctaggccatt tccaggtcct gtacctggcc cctcgtcaga catgattcac
     7201 actaaaagag atcaatagac atctttatta gacgacgctc agtgaataca gggagtgcag
     7261 actcctgccc cctccaacag cccccccacc ctcatcccct tcatggtcgc tgtcagacag
     7321 atccaggtct gaaaattccc catcctccga accatcctcg tcctcatcac caattactcg
     7381 cagcccggaa aactcccgct gaacatcctc aagatttgcg tcctgagcct caagccaggc
     7441 ctcaaattcc tcgtccccct ttttgctgga cggtagggat ggggattctc gggacccctc
     7501 ctcttcctct tcaaggtcac cagacagaga tgctactggg gcaacggaag aaaagctggg
     7561 tgcggcctgt gaggatcagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga
     7621 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     7681 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     7741 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     7801 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     7861 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     7921 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     7981 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     8041 tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac
     8101 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     8161 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     8221 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     8281 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     8341 gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc
     8401 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     8461 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     8521 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     8581 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     8641 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     8701 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     8761 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     8821 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     8881 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     8941 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     9001 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     9061 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     9121 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     9181 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     9241 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     9301 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     9361 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     9421 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     9481 tggccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac
     9541 gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct
     9601 cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccccgagatg cgccgcgtgc
     9661 ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca
     9721 cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt
     9781 gccgccctgc ttcatccccg tggcccgttg ctcgcgtttg ctggcggtgt ccccggaaga
     9841 aatatatttg catgtcttta gttctatgat gacacaaacc ccgcccagcg tcttgtcatt
     9901 ggcgaattcg aacacgcaga tgcagtcggg gcggcgcggt ccgaggtcca cttcgcatat
     9961 taaggtgacg cgtgtggcct cgaacaccga gcgaccctgc agcgacccgc ttaacagcgt
    10021 caacagcgtg ccgcagatcc cggggggcaa tgagatatga aaaagcctga actcaccgcg
    10081 acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc
    10141 tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg
    10201 cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca
    10261 tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga gagcctgacc
    10321 tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg
    10381 cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc cgatcttagc
    10441 cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt
    10501 gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac
    10561 accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc
    10621 cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat
    10681 ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag
    10741 gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac
    10801 ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta tatgctccgc
    10861 attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg
    10921 gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa
    10981 atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt
    11041 ggaaaccgac gccccagcac tcgtccggat cgggagatgg gggaggctaa ctgaaacacg
    11101 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac
    11161 gcacgggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg
    11221 tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac
    11281 cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc
    11341 ctgccatagc cactggcccc gtgggttagg gacggggtcc cccatgggga atggtttatg
    11401 gttcgtgggg gttattattt tgggcgttgc gtggggtcag gtccacgact ggactgagca
    11461 gacagaccca tggtttttgg atggcctggg catggaccgc atgtactggc gcgacacgaa
    11521 caccgggcgt ctgtggctgc caaacacccc cgacccccaa aaaccaccgc gcggatttct
    11581 ggcgtgccaa gctagtcgac caattctcat gtttgacagc ttatcatcgc agatccgggc
    11641 aacgttgttg ccattgctgc aggcgcagaa ctggtaggta tggaagatct atacattgaa
    11701 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
    11761 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
    11821 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
    11881 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
    11941 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
    12001 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
    12061 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
    12121 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
    12181 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
    12241 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
    12301 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
    12361 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
    12421 tcgtttagtg aaccgtcaga tctctagaag ctgggtacca gctgctagc
//
