LOCUS       sequence-235167-       12799 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the ZM197M HIV-1 isolate; 
            QES mutant with enhanced binding to PG16.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12799)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 12799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-ZM197M-QES.c10
FEATURES             Location/Qualifiers
     source          1..12799
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    2648..2782
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2672..2691)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2726..2745
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2925..2947)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      3507..5296
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(5598..7523)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(7958..7976)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8044..8148
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8149..8217,8218..9009)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8367..8386)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9180..9768
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9669..9688
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        10199..10344
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             10387..11424
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(11422..11441)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11466..11514
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        12168..12547
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12548..12751
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     12702..12722
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     12748..12772
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gccaccatgc ccatggggag tctccaaccg ctggcaacgc tgtatcttct cggtatgttg
       61 gtggcgtcag tcttggcgga acaactgtgg gttacggtgt attacggtgt tccggtgtgg
      121 aaagaggcta aagcaactct cttctgcgcc agcgatgcga aagcctacga aaaagaagta
      181 cataacgtgt gggccaccca tgcttgcgta cccacagatc cgaatcccca agagataccc
      241 ttgggtaacg tgacggaaaa tttcaatatg tggaagaacg acatggcaga ccaaatgcac
      301 gaagacataa ttagtttgtg ggaccaaagc ttgaaaccat gcgtcaaact gaccccattg
      361 tgcgtgactc tgaattgcag cgacgctaca agtaatacga caaagaatgc gactaacaca
      421 aacacgactt caacagacaa ccgaaatgcg acaagtaacg ataccgaaat gaaaggggaa
      481 attaaagatt gtacctttaa tatcacaact gaagtccgag acagaaagac taagcaacgc
      541 gcactgttct ataaacttga tgtggtccct ctggaggagg agaaaaactc cagctctaaa
      601 aacagctctt acaaagagta ccgattgatt agctgcaaca cgagcacaat tacccaagcc
      661 tgtccaaaaa tgtcttttga cccaattccg atccactact gcgctccagc aggctatgcg
      721 atacttaaat gtaacaataa aacattcaac ggtaccggac catgtcataa cgtcagtacg
      781 gttcagtgca cgcacgggat aaaaccaact atgtctacac aactgcttct taacggttct
      841 cttgccgagg aggaaatcat catacggtct gagaacctga ctgacaacac taaaacaatt
      901 atagtgcatt tgaacgagtc agtcgagatt gaatgcgtta ggcccaataa taacacgagg
      961 aaaagcgtca gaatcggtcc cgggcaaact ttcttcgcta caggggaaat cataggtgac
     1021 attcgccagg cgcattgcga tctttcaaag tctaattgga ccacgacgct taaaaggatt
     1081 gagaagaaat tgaaggaaca cttcaataac gctaccatca agtttgaatc ttccgctggc
     1141 ggcgaccttg agatcactac ccattctttt aattgtcgcg gcgagttctt ctattgtaat
     1201 acgtctggcc tcttcaactc ttctctgctg aacgatacag acggaacgag caactctact
     1261 tctaatgcaa ccatcacgct cccgtgccgc ataaaacaaa tcatcaatat gtggcaggaa
     1321 gtggggcggg caatgtacgc ctcaccgatt gccgggatca taacatgcaa aagtaacatc
     1381 accgggctct tgctcacacg agacggtggg aataaaagcg cagggataga gacctttcgc
     1441 ccagggggcg ggaacatgaa agataattgg cgatccgagc tgtataaata caaggtggtt
     1501 gaaatcaaac cccttgggat tgcacctact agcgcgaaac ggcgggtcgt cgaaagagag
     1561 aaaagagccg cggggatcgg cgcggtaata ttgggctttc tcggcgcagc gggttcaact
     1621 atgggcgctg ctagtgtgat gcttactgtc caggcacgac agttgttgag tggtattgtt
     1681 cagcaacaat ctaacctgct tcgcgctatc gaggcacaac agcatatgtt gcaacttaca
     1741 gtatggggaa ttaaacaact gcaaactcgg gtactggcta tcgagcgcta ccttaaggat
     1801 cagcaattgc ttggtttgtg ggggtgcagt ggtaaactga tctgtaccac agcggtaccc
     1861 tggaacacca gttggtctaa caaaagcaaa gacgaaatat gggacaacat gacatggatg
     1921 cagtgggatc gggaaataga taactacacc caggtcatct accaacttct cgaagtatct
     1981 cagaatcagc aggagaagaa cgagaatgat ctccttgctt tggataagtg gaacagcctg
     2041 tggagttggt ttgatataac gaagtggctc tggtacatta agatatttat catgatcgtt
     2101 gggggtttga ttggtttgag gattattttt gctgtattgt cagtggtcaa tcgggtacgg
     2161 cagggttatt ccccgctctc cttccaaacg ctgattccca atccacgggg acccgacaga
     2221 ccaggaggta ttgaggaaga gggtggcgag caagataaga ataggtcaac gagattggtt
     2281 agcggctttt tggctcttgt atgggatgac ctgcgctcct tgtgtctttt ttcttaccat
     2341 cgactccgag acttcattct tatcgctgcg cgcgctgtcg aacttttggg gaggagcagc
     2401 ctggaaggcc ttcagtgggg ctgggagaca cttaaatatc ttcgcaatct cgtccagtat
     2461 tgggggttgg aacttaaaca gtcagcgatc aacttgctcg acacgatcgc tatccaagtg
     2521 gctgagggca cggacagaat tatagaactg atccagagaa ttttccgggc gatctgtaat
     2581 atacctacgc gaataaggca ggggtttgag gcagccctcc agtagtaact cgaggccggc
     2641 aaggccggat ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     2701 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     2761 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
     2821 gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga
     2881 ttatgatccg gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     2941 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     3001 ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgaggtcgac tctagaggat
     3061 cgatccccgc cccggacgaa ctaaacctga ctacgacatc tctgcccctt cttcgcgggg
     3121 cagtgcatgt aatcccttca gttggttggt acaacttgcc aactgggccc tgttccacat
     3181 gtgacacggg gggggaccaa acacaaaggg gttctctgac tgtagttgac atccttataa
     3241 atggatgtgc acatttgcca acactgagtg gctttcatcc tggagcagac tttgcagtct
     3301 gtggactgca acacaacatt gcctttatgt gtaactcttg gctgaagctc ttacaccaat
     3361 gctgggggac atgtacctcc caggggccca ggaagactac gggaggctac accaacgtca
     3421 atcagagggg cctgtgtagc taccgataag cggaccctca agagggcatt agcaatagtg
     3481 tttataaggc ccccttgtta accctaaacg ggtagcatat gcttcccggg tagtagtata
     3541 tactatccag actaacccta attcaatagc atatgttacc caacgggaag catatgctat
     3601 cgaattaggg ttagtaaaag ggtcctaagg aacagcgata tctcccaccc catgagctgt
     3661 cacggtttta tttacatggg gtcaggattc cacgagggta gtgaaccatt ttagtcacaa
     3721 gggcagtggc tgaagatcaa ggagcgggca gtgaactctc ctgaatcttc gcctgcttct
     3781 tcattctcct tcgtttagct aatagaataa ctgctgagtt gtgaacagta aggtgtatgt
     3841 gaggtgctcg aaaacaaggt ttcaggtgac gcccccagaa taaaatttgg acggggggtt
     3901 cagtggtggc attgtgctat gacaccaata taaccctcac aaaccccttg ggcaataaat
     3961 actagtgtag gaatgaaaca ttctgaatat ctttaacaat agaaatccat ggggtgggga
     4021 caagccgtaa agactggatg tccatctcac acgaatttat ggctatgggc aacacataat
     4081 cctagtgcaa tatgatactg gggttattaa gatgtgtccc aggcagggac caagacaggt
     4141 gaaccatgtt gttacactct atttgtaaca aggggaaaga gagtggacgc cgacagcagc
     4201 ggactccact ggttgtctct aacacccccg aaaattaaac ggggctccac gccaatgggg
     4261 cccataaaca aagacaagtg gccactcttt tttttgaaat tgtggagtgg gggcacgcgt
     4321 cagcccccac acgccgccct gcggttttgg actgtaaaat aagggtgtaa taacttggct
     4381 gattgtaacc ccgctaacca ctgcggtcaa accacttgcc cacaaaacca ctaatggcac
     4441 cccggggaat acctgcataa gtaggtgggc gggccaagat aggggcgcga ttgctgcgat
     4501 ctggaggaca aattacacac acttgcgcct gagcgccaag cacagggttg ttggtcctca
     4561 tattcacgag gtcgctgaga gcacggtggg ctaatgttgc catgggtagc atatactacc
     4621 caaatatctg gatagcatat gctatcctaa tctatatctg ggtagcatag gctatcctaa
     4681 tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa
     4741 tttatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa
     4801 tctatatctg ggtagtatat gctatcctaa tctgtatccg ggtagcatat gctatcctaa
     4861 tagagattag ggtagtatat gctatcctaa tttatatctg ggtagcatat actacccaaa
     4921 tatctggata gcatatgcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
     4981 tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
     5041 tatctgggta gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta
     5101 tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg
     5161 tatccgggta gcatatgcta tcctcatgca tatacagtca gcatatgata cccagtagta
     5221 gagtgggagt gctatccttt gcatatgccg ccacctccca agggggcgtg aattttcgct
     5281 gcttgtcctt ttcctgctgg ttgctcccat tcttaggtga atttaaggag gccaggctaa
     5341 agccgtcgca tgtctgattg ctcaccaggt aaatgtcgct aatgttttcc aacgcgagaa
     5401 ggtgttgagc gcggagctga gtgacgtgac aacatgggta tgcccaattg ccccatgttg
     5461 ggaggacgaa aatggtgaca agacagatgg ccagaaatac accaacagca cgcatgatgt
     5521 ctactgggga tttattcttt agtgcggggg aatacacggc ttttaatacg attgagggcg
     5581 tctcctaaca agttacatca ctcctgccct tcctcaccct catctccatc acctccttca
     5641 tctccgtcat ctccgtcatc accctccgcg gcagcccctt ccaccatagg tggaaaccag
     5701 ggaggcaaat ctactccatc gtcaaagctg cacacagtca ccctgatatt gcaggtagga
     5761 gcgggctttg tcataacaag gtccttaatc gcatccttca aaacctcagc aaatatatga
     5821 gtttgtaaaa agaccatgaa ataacagaca atggactccc ttagcgggcc aggttgtggg
     5881 ccgggtccag gggccattcc aaaggggaga cgactcaatg gtgtaagacg acattgtgga
     5941 atagcaaggg cagttcctcg ccttaggttg taaagggagg tcttactacc tccatatacg
     6001 aacacaccgg cgacccaagt tccttcgtcg gtagtccttt ctacgtgact cctagccagg
     6061 agagctctta aaccttctgc aatgttctca aatttcgggt tggaacctcc ttgaccacga
     6121 tgctttccaa accaccctcc ttttttgcgc ctgcctccat caccctgacc ccggggtcca
     6181 gtgcttgggc cttctcctgg gtcatctgcg gggccctgct ctatcgctcc cgggggcacg
     6241 tcaggctcac catctgggcc accttcttgg tggtattcaa aataatcggc ttcccctaca
     6301 gggtggaaaa atggccttct acctggaggg ggcctgcgcg gtggagaccc ggatgatgat
     6361 gactgactac tgggactcct gggcctcttt tctccacgtc cacgacctct ccccctggct
     6421 ctttcacgac ttccccccct ggctctttca cgtcctctac cccggcggcc tccactacct
     6481 cctcgacccc ggcctccact acctcctcga ccccggcctc cactgcctcc tcgaccccgg
     6541 cctccacctc ctgctcctgc ccctcctgct cctgcccctc ctcctgctcc tgcccctcct
     6601 gcccctcctg ctcctgcccc tcctgcccct cctgctcctg cccctcctgc ccctcctgct
     6661 cctgcccctc ctgcccctcc tcctgctcct gcccctcctg cccctcctcc tgctcctgcc
     6721 cctcctgccc ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc tcctgcccct
     6781 cctgctcctg cccctcctgc tcctgcccct cctgctcctg cccctcctgc tcctgcccct
     6841 cctgcccctc ctgcccctcc tcctgctcct gcccctcctg ctcctgcccc tcctgcccct
     6901 cctgcccctc ctgctcctgc ccctcctcct gctcctgccc ctcctgcccc tcctgcccct
     6961 cctcctgctc ctgcccctcc tgcccctcct cctgctcctg cccctcctcc tgctcctgcc
     7021 cctcctgccc ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc tcctgctcct
     7081 gcccctcctc ctgctcctgc ccctcctgcc cctcctgccc ctcctcctgc tcctgcccct
     7141 cctcctgctc ctgcccctcc tgcccctcct gcccctcctg cccctcctcc tgctcctgcc
     7201 cctcctcctg ctcctgcccc tcctgctcct gcccctcccg ctcctgctcc tgctcctgtt
     7261 ccaccgtggg tccctttgca gccaatgcaa cttggacgtt tttggggtct ccggacacca
     7321 tctctatgtc ttggccctga tcctgagccg cccggggctc ctggtcttcc gcctcctcgt
     7381 cctcgtcctc ttccccgtcc tcgtccatgg ttatcacccc ctcttctttg aggtccactg
     7441 ccgccggagc cttctggtcc agatgtgtct cccttctctc ctaggccatt tccaggtcct
     7501 gtacctggcc cctcgtcaga catgattcac actaaaagag atcaatagac atctttatta
     7561 gacgacgctc agtgaataca gggagtgcag actcctgccc cctccaacag cccccccacc
     7621 ctcatcccct tcatggtcgc tgtcagacag atccaggtct gaaaattccc catcctccga
     7681 accatcctcg tcctcatcac caattactcg cagcccggaa aactcccgct gaacatcctc
     7741 aagatttgcg tcctgagcct caagccaggc ctcaaattcc tcgtccccct ttttgctgga
     7801 cggtagggat ggggattctc gggacccctc ctcttcctct tcaaggtcac cagacagaga
     7861 tgctactggg gcaacggaag aaaagctggg tgcggcctgt gaggatcagc ttatcgatga
     7921 taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta
     7981 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     8041 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     8101 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     8161 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     8221 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     8281 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     8341 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
     8401 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     8461 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     8521 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     8581 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     8641 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg
     8701 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     8761 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     8821 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     8881 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     8941 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     9001 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     9061 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     9121 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     9181 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     9241 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     9301 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     9361 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     9421 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     9481 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     9541 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     9601 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     9661 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     9721 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     9781 gcggcctttt tacggttcct ggccttttgc tggccttgaa gctgtccctg atggtcgtca
     9841 tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga
     9901 agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc
     9961 agcgcgtcgg ccccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata
    10021 tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa
    10081 ttcttggagt ggtgaatccg ttagcgaggt gccgccctgc ttcatccccg tggcccgttg
    10141 ctcgcgtttg ctggcggtgt ccccggaaga aatatatttg catgtcttta gttctatgat
    10201 gacacaaacc ccgcccagcg tcttgtcatt ggcgaattcg aacacgcaga tgcagtcggg
    10261 gcggcgcggt ccgaggtcca cttcgcatat taaggtgacg cgtgtggcct cgaacaccga
    10321 gcgaccctgc agcgacccgc ttaacagcgt caacagcgtg ccgcagatcc cggggggcaa
    10381 tgagatatga aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag
    10441 ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc
    10501 ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac
    10561 aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt
    10621 gacattgggg aattcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc
    10681 acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc
    10741 atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg
    10801 caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat
    10861 gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc
    10921 gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat
    10981 ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc
    11041 gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg
    11101 ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga
    11161 tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg
    11221 gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga
    11281 tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc
    11341 gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccggat
    11401 cgggagatgg gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc
    11461 tatgacggca ataaaaagac agaataaaac gcacgggtgt tgggtcgttt gttcataaac
    11521 gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc
    11581 aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag
    11641 ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc cactggcccc gtgggttagg
    11701 gacggggtcc cccatgggga atggtttatg gttcgtgggg gttattattt tgggcgttgc
    11761 gtggggtcag gtccacgact ggactgagca gacagaccca tggtttttgg atggcctggg
    11821 catggaccgc atgtactggc gcgacacgaa caccgggcgt ctgtggctgc caaacacccc
    11881 cgacccccaa aaaccaccgc gcggatttct ggcgtgccaa gctagtcgac caattctcat
    11941 gtttgacagc ttatcatcgc agatccgggc aacgttgttg ccattgctgc aggcgcagaa
    12001 ctggtaggta tggaagatct atacattgaa tcaatattgg caattagcca tattagtcat
    12061 tggttatata gcataaatca atattggcta ttggccattg catacgttgt atctatatca
    12121 taatatgtac atttatattg gctcatgtcc aatatgaccg ccatgttgac attgattatt
    12181 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
    12241 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
    12301 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
    12361 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
    12421 gccaagtccg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
    12481 gtacatgacc ttacgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
    12541 taccatggtg atgcggtttt ggcagtacac caatgggcgt ggatagcggt ttgactcacg
    12601 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
    12661 acgggacttt ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg gcggtaggcg
    12721 tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga tctctagaag
    12781 ctgggtacca gctgctagc
//
