LOCUS       sequence-235171-       12412 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the Q769.d22 HIV-1 
            isolate; C-terminal truncation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12412)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 12412)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-Q769gp160-754*
FEATURES             Location/Qualifiers
     source          1..12412
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             1637..1645
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    2261..2395
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2285..2304)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2339..2358
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2538..2560)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      3120..4909
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(5211..7136)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(7571..7589)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7657..7761
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7762..7830,7831..8622)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7980..7999)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8793..9381
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9282..9301
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        9812..9957
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             10000..11037
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(11035..11054)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11079..11127
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        11781..12160
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12161..12364
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     12315..12335
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     12361..12385
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gccaccatgc ccatggggtc tttgcaaccc ctcgctactc tttatttgct gggtatgttg
       61 gtagcgagcg ttctggcggc gggcaatctc tgggtaaccg tgtactacgg cgtgccagtg
      121 tggcgggatg ctgaaacaac cctcttttgt gcaagtgatg caaaagcgta tgaccgagaa
      181 gcacacaatg tttgggctac acatgcgtgc gttccgacag atccatcccc ccaagaagta
      241 ccattgggga atgtaaccga ggaattcaac atgtggaaga ataacatggt tgaacagatg
      301 catacagaca tcatctcttt gtgggaccag tcactgcagc cgtgtgtgaa acttacgcct
      361 ctttgtgtca cgctgaattg ttccaatagt aataacattc caagtgtcag caacattacc
      421 gatgatatga aagaagagat taagaactgc agctttaaca tgacaaccga attgaaggat
      481 aaaaagcaga acgtttactc tttgttctat cgactggacg ttgtacccct cgaaacaaaa
      541 accaaccaga acagtagtca ctcaaggtat cggctcatta actgcaacac gtctgccatc
      601 acgcaggctt gcccaaaagt ttctttcgag ccgatcccta ttcactattg tgcgcctgca
      661 ggctttgcta tcctgaaatg taacgacaag ggctttaacg gcactggact ttgtaaaaac
      721 gtctcaacag tacaatgcac gcatgggatt aaaccggtcg tcagtaccca gctgctcctt
      781 aatggaagcc ttgccgaggg taaggtaatg gtacgatccg aaaatattac aaataatgcg
      841 aaaaacatta taatacagtt taacaactct gtgcaaatta attgcacgcg cccaggtaat
      901 aacacgcgaa aaagtataca tctcgggcct gggaaagttt tttacgcgac ggacataata
      961 ggggatataa ggaaagcgca ctgtaatgtt aatcgccaac aatggaataa gacccttcag
     1021 gatgtggcaa cgcaactcag gacccacttc agaaaccgta cgatcatatt taataatagt
     1081 ctcggtgggg atttggaaat aactacgcat agttttaatt gtaggggaga gtttttctat
     1141 tgtaacactt ccgggctctt taatgggata tggaacggaa cgcaagaacc taatcggacc
     1201 gagtctaacg atacaattac gcttcaatgt cggataaagc agatcatcaa catgtggcag
     1261 agagtaggcc aggcaattta cgcaccccct atccaggggg agatcagatg cgaaagtaac
     1321 ataaccggac ttatcctgac gagggatggg gggatcatca acagtacaga ggaaacgttt
     1381 agacctgggg gcggggacat gagagataat tggaggtccg agctctataa atacaaagtt
     1441 gtaaagatag aaccgcttgg agtggctcca actaaggcca agagacgggt cgtggagaga
     1501 gaaaaacggg cggtaggatt tggcgcgttc tttctcggtt tccttggcgc agccggatca
     1561 acgatggggg ccgcctccat caccctgacg gttcaggcgc gccagctttt gagtgggatt
     1621 gtgcagcagc agaataacct ccttagggcc atagaggctc agcagcacct gctgaagctt
     1681 accgtttggg gcattaagca actgcaggcg cgggtactcg cggttgaaag ataccttaag
     1741 gatcagcaac tgctgggaat ttggggatgt agtggaaagt tcatctgcac aaccacggtc
     1801 ccatggaatt cctcatggtc aaataaatca cagtcagaaa tatgggataa tatgacgtgg
     1861 atgcagtggg acaaggagat taacaattac acccagatta tctacgatct tattgaggag
     1921 tcacaaaggc aacaagagaa aaacgaacag gacttgctgg cgctcgacaa atgggctaat
     1981 ttgtggaact ggttcgacat cagtaactgg ctgtggtaca taaagatttt cataatgata
     2041 gttggcgggc tcatagggct tcggattgcg ttcgcagtgc tttctgtcat caatagggtc
     2101 aggcaaggtt attccccgct ctcctttcaa acccataccc caaaccctcg ggatttggat
     2161 aggccaggcc ggatagagga ggaaggaggt gagcaggatc gggaccgaag cataagactg
     2221 gtctctgggt ttctttgata actcgaggcc ggcaaggccg gatccagaca tgataagata
     2281 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     2341 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     2401 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
     2461 caagtaaaac ctctacaaat gtggtatggc tgattatgat ccggctgcct cgcgcgtttc
     2521 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     2581 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     2641 cggggcgcag ccatgaggtc gactctagag gatcgatccc cgccccggac gaactaaacc
     2701 tgactacgac atctctgccc cttcttcgcg gggcagtgca tgtaatccct tcagttggtt
     2761 ggtacaactt gccaactggg ccctgttcca catgtgacac ggggggggac caaacacaaa
     2821 ggggttctct gactgtagtt gacatcctta taaatggatg tgcacatttg ccaacactga
     2881 gtggctttca tcctggagca gactttgcag tctgtggact gcaacacaac attgccttta
     2941 tgtgtaactc ttggctgaag ctcttacacc aatgctgggg gacatgtacc tcccaggggc
     3001 ccaggaagac tacgggaggc tacaccaacg tcaatcagag gggcctgtgt agctaccgat
     3061 aagcggaccc tcaagagggc attagcaata gtgtttataa ggcccccttg ttaaccctaa
     3121 acgggtagca tatgcttccc gggtagtagt atatactatc cagactaacc ctaattcaat
     3181 agcatatgtt acccaacggg aagcatatgc tatcgaatta gggttagtaa aagggtccta
     3241 aggaacagcg atatctccca ccccatgagc tgtcacggtt ttatttacat ggggtcagga
     3301 ttccacgagg gtagtgaacc attttagtca caagggcagt ggctgaagat caaggagcgg
     3361 gcagtgaact ctcctgaatc ttcgcctgct tcttcattct ccttcgttta gctaatagaa
     3421 taactgctga gttgtgaaca gtaaggtgta tgtgaggtgc tcgaaaacaa ggtttcaggt
     3481 gacgccccca gaataaaatt tggacggggg gttcagtggt ggcattgtgc tatgacacca
     3541 atataaccct cacaaacccc ttgggcaata aatactagtg taggaatgaa acattctgaa
     3601 tatctttaac aatagaaatc catggggtgg ggacaagccg taaagactgg atgtccatct
     3661 cacacgaatt tatggctatg ggcaacacat aatcctagtg caatatgata ctggggttat
     3721 taagatgtgt cccaggcagg gaccaagaca ggtgaaccat gttgttacac tctatttgta
     3781 acaaggggaa agagagtgga cgccgacagc agcggactcc actggttgtc tctaacaccc
     3841 ccgaaaatta aacggggctc cacgccaatg gggcccataa acaaagacaa gtggccactc
     3901 ttttttttga aattgtggag tgggggcacg cgtcagcccc cacacgccgc cctgcggttt
     3961 tggactgtaa aataagggtg taataacttg gctgattgta accccgctaa ccactgcggt
     4021 caaaccactt gcccacaaaa ccactaatgg caccccgggg aatacctgca taagtaggtg
     4081 ggcgggccaa gataggggcg cgattgctgc gatctggagg acaaattaca cacacttgcg
     4141 cctgagcgcc aagcacaggg ttgttggtcc tcatattcac gaggtcgctg agagcacggt
     4201 gggctaatgt tgccatgggt agcatatact acccaaatat ctggatagca tatgctatcc
     4261 taatctatat ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc
     4321 taatctatat ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc
     4381 taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc
     4441 taatctgtat ccgggtagca tatgctatcc taatagagat tagggtagta tatgctatcc
     4501 taatttatat ctgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat
     4561 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagcatagg ctatcctaat
     4621 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     4681 ttatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     4741 ctatatctgg gtagtatatg ctatcctaat ctgtatccgg gtagcatatg ctatcctcat
     4801 gcatatacag tcagcatatg atacccagta gtagagtggg agtgctatcc tttgcatatg
     4861 ccgccacctc ccaagggggc gtgaattttc gctgcttgtc cttttcctgc tggttgctcc
     4921 cattcttagg tgaatttaag gaggccaggc taaagccgtc gcatgtctga ttgctcacca
     4981 ggtaaatgtc gctaatgttt tccaacgcga gaaggtgttg agcgcggagc tgagtgacgt
     5041 gacaacatgg gtatgcccaa ttgccccatg ttgggaggac gaaaatggtg acaagacaga
     5101 tggccagaaa tacaccaaca gcacgcatga tgtctactgg ggatttattc tttagtgcgg
     5161 gggaatacac ggcttttaat acgattgagg gcgtctccta acaagttaca tcactcctgc
     5221 ccttcctcac cctcatctcc atcacctcct tcatctccgt catctccgtc atcaccctcc
     5281 gcggcagccc cttccaccat aggtggaaac cagggaggca aatctactcc atcgtcaaag
     5341 ctgcacacag tcaccctgat attgcaggta ggagcgggct ttgtcataac aaggtcctta
     5401 atcgcatcct tcaaaacctc agcaaatata tgagtttgta aaaagaccat gaaataacag
     5461 acaatggact cccttagcgg gccaggttgt gggccgggtc caggggccat tccaaagggg
     5521 agacgactca atggtgtaag acgacattgt ggaatagcaa gggcagttcc tcgccttagg
     5581 ttgtaaaggg aggtcttact acctccatat acgaacacac cggcgaccca agttccttcg
     5641 tcggtagtcc tttctacgtg actcctagcc aggagagctc ttaaaccttc tgcaatgttc
     5701 tcaaatttcg ggttggaacc tccttgacca cgatgctttc caaaccaccc tccttttttg
     5761 cgcctgcctc catcaccctg accccggggt ccagtgcttg ggccttctcc tgggtcatct
     5821 gcggggccct gctctatcgc tcccgggggc acgtcaggct caccatctgg gccaccttct
     5881 tggtggtatt caaaataatc ggcttcccct acagggtgga aaaatggcct tctacctgga
     5941 gggggcctgc gcggtggaga cccggatgat gatgactgac tactgggact cctgggcctc
     6001 ttttctccac gtccacgacc tctccccctg gctctttcac gacttccccc cctggctctt
     6061 tcacgtcctc taccccggcg gcctccacta cctcctcgac cccggcctcc actacctcct
     6121 cgaccccggc ctccactgcc tcctcgaccc cggcctccac ctcctgctcc tgcccctcct
     6181 gctcctgccc ctcctcctgc tcctgcccct cctgcccctc ctgctcctgc ccctcctgcc
     6241 cctcctgctc ctgcccctcc tgcccctcct gctcctgccc ctcctgcccc tcctcctgct
     6301 cctgcccctc ctgcccctcc tcctgctcct gcccctcctg cccctcctgc tcctgcccct
     6361 cctgcccctc ctgctcctgc ccctcctgcc cctcctgctc ctgcccctcc tgctcctgcc
     6421 cctcctgctc ctgcccctcc tgctcctgcc cctcctgccc ctcctgcccc tcctcctgct
     6481 cctgcccctc ctgctcctgc ccctcctgcc cctcctgccc ctcctgctcc tgcccctcct
     6541 cctgctcctg cccctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctgcccct
     6601 cctcctgctc ctgcccctcc tcctgctcct gcccctcctg cccctcctgc ccctcctcct
     6661 gctcctgccc ctcctgcccc tcctcctgct cctgcccctc ctcctgctcc tgcccctcct
     6721 gcccctcctg cccctcctcc tgctcctgcc cctcctcctg ctcctgcccc tcctgcccct
     6781 cctgcccctc ctgcccctcc tcctgctcct gcccctcctc ctgctcctgc ccctcctgct
     6841 cctgcccctc ccgctcctgc tcctgctcct gttccaccgt gggtcccttt gcagccaatg
     6901 caacttggac gtttttgggg tctccggaca ccatctctat gtcttggccc tgatcctgag
     6961 ccgcccgggg ctcctggtct tccgcctcct cgtcctcgtc ctcttccccg tcctcgtcca
     7021 tggttatcac cccctcttct ttgaggtcca ctgccgccgg agccttctgg tccagatgtg
     7081 tctcccttct ctcctaggcc atttccaggt cctgtacctg gcccctcgtc agacatgatt
     7141 cacactaaaa gagatcaata gacatcttta ttagacgacg ctcagtgaat acagggagtg
     7201 cagactcctg ccccctccaa cagccccccc accctcatcc ccttcatggt cgctgtcaga
     7261 cagatccagg tctgaaaatt ccccatcctc cgaaccatcc tcgtcctcat caccaattac
     7321 tcgcagcccg gaaaactccc gctgaacatc ctcaagattt gcgtcctgag cctcaagcca
     7381 ggcctcaaat tcctcgtccc cctttttgct ggacggtagg gatggggatt ctcgggaccc
     7441 ctcctcttcc tcttcaaggt caccagacag agatgctact ggggcaacgg aagaaaagct
     7501 gggtgcggcc tgtgaggatc agcttatcga tgataagctg tcaaacatga gaattcttga
     7561 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     7621 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     7681 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     7741 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     7801 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     7861 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     7921 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     7981 ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata
     8041 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     8101 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     8161 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     8221 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     8281 gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact
     8341 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     8401 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     8461 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     8521 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     8581 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     8641 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     8701 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     8761 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     8821 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     8881 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     8941 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     9001 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     9061 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     9121 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     9181 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     9241 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     9301 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     9361 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     9421 tgctggcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc
     9481 aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag
     9541 cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccccgag atgcgccgcg
     9601 tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat
     9661 tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga
     9721 ggtgccgccc tgcttcatcc ccgtggcccg ttgctcgcgt ttgctggcgg tgtccccgga
     9781 agaaatatat ttgcatgtct ttagttctat gatgacacaa accccgccca gcgtcttgtc
     9841 attggcgaat tcgaacacgc agatgcagtc ggggcggcgc ggtccgaggt ccacttcgca
     9901 tattaaggtg acgcgtgtgg cctcgaacac cgagcgaccc tgcagcgacc cgcttaacag
     9961 cgtcaacagc gtgccgcaga tcccgggggg caatgagata tgaaaaagcc tgaactcacc
    10021 gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag
    10081 ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc
    10141 ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt
    10201 gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg
    10261 acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa
    10321 ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt
    10381 agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg
    10441 cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac
    10501 gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac
    10561 tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac
    10621 aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac
    10681 gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc
    10741 tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc
    10801 cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct
    10861 tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca
    10921 caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat
    10981 agtggaaacc gacgccccag cactcgtccg gatcgggaga tgggggaggc taactgaaac
    11041 acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa
    11101 aacgcacggg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact
    11161 ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc
    11221 caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag
    11281 gccctgccat agccactggc cccgtgggtt agggacgggg tcccccatgg ggaatggttt
    11341 atggttcgtg ggggttatta ttttgggcgt tgcgtggggt caggtccacg actggactga
    11401 gcagacagac ccatggtttt tggatggcct gggcatggac cgcatgtact ggcgcgacac
    11461 gaacaccggg cgtctgtggc tgccaaacac ccccgacccc caaaaaccac cgcgcggatt
    11521 tctggcgtgc caagctagtc gaccaattct catgtttgac agcttatcat cgcagatccg
    11581 ggcaacgttg ttgccattgc tgcaggcgca gaactggtag gtatggaaga tctatacatt
    11641 gaatcaatat tggcaattag ccatattagt cattggttat atagcataaa tcaatattgg
    11701 ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata ttggctcatg
    11761 tccaatatga ccgccatgtt gacattgatt attgactagt tattaatagt aatcaattac
    11821 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
    11881 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
    11941 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
    12001 tgcccacttg gcagtacatc aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa
    12061 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttacggg actttcctac
    12121 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
    12181 caccaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
    12241 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa
    12301 ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
    12361 agctcgttta gtgaaccgtc agatctctag aagctgggta ccagctgcta gc
//
