LOCUS       sequence-235175-       12742 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the Q769.d22 HIV-1 
            isolate; QES mutant with enhanced binding to PG16.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12742)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 12742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-Q769-QES.i03.c04
FEATURES             Location/Qualifiers
     source          1..12742
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             1637..1645
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    2591..2725
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2615..2634)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2669..2688
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2868..2890)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      3450..5239
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(5541..7466)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(7901..7919)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7987..8091
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8092..8160,8161..8952)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8310..8329)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9123..9711
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9612..9631
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        10142..10287
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             10330..11367
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(11365..11384)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11409..11457
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        12111..12490
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12491..12694
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     12645..12665
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     12691..12715
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gccaccatgc ccatggggtc tttgcaaccc ctcgctactc tttatttgct gggtatgttg
       61 gtagcgagcg ttctggcggc gggcaatctc tgggtaaccg tgtactacgg cgtgccagtg
      121 tggcgggatg ctgaaacaac cctcttttgt gcaagtgatg caaaagcgta tgaccgagaa
      181 gcacacaatg tttgggctac acatgcgtgc gttccgacag atccatcccc ccaagaagta
      241 ccattgggga atgtaaccga ggaattcaac atgtggaaga ataacatggt tgaacagatg
      301 catacagaca tcatctcttt gtgggaccag tcactgcagc cgtgtgtgaa acttacgcct
      361 ctttgtgtca cgctgaattg ttccaatagt aataacattc caagtgtcag caacattacc
      421 gatgatatga aagaagagat taagaactgc agctttaaca tgacaaccga attgaaggat
      481 aaaaagcaga acgtttactc tttgttctat cgactggacg ttgtacccct cgaaacaaaa
      541 accaaccaga acagtagtca ctcaaggtat cggctcatta actgcaacac gtctgagatc
      601 acgcaggctt gcccaaaagt ttctttcgag ccgatcccta ttcactattg tgcgcctgca
      661 ggctatgcta tcctgaaatg taacgacaag ggctttaacg gcactggact ttgtaaaaac
      721 gtctcaacag tacaatgcac gcatgggatt aaaccggtca tgagtaccca gctgctcctt
      781 aatggaagcc ttgccgaggg taaggtaatg gtacgatccg aaaatattac aaataatgcg
      841 aaaaacatta taatacagtt taacaactct gtgcaaatta attgcacgcg cccaggtaat
      901 aacacgcgaa aaagtataca tctcgggcct gggaaagttt tttacgcgac ggacataata
      961 ggggatataa ggaaagcgca ctgtaatgtt aatcgccaac aatggaataa gacccttcag
     1021 gatgtggcaa cgcaactcag gacccacttc agaaaccgta cgatcatatt taataatagt
     1081 ctcggtgggg atttggaaat aactacgcat agttttaatt gtaggggaga gtggttctat
     1141 tgtaacactt ccgggctctt taatgggata tggaacggaa cgcaagaacc taatcggacc
     1201 gagtctaacg atacaattac gcttcaatgt cggataaagc agatcatcaa catgtggcag
     1261 agagtaggcc aggcaattta cgcaccccct atccaggggg agatcagatg cgaaagtaac
     1321 ataaccggac ttatcctgac gagggatggg gggatcatca acagtacaga ggaaacgttt
     1381 agacctgggg gcggggacat gagagataat tggaggtccg agctctataa atacaaagtt
     1441 gtaaagatag aaccgcttgg agtggctcca actaaggcca agagacgggt cgtggagaga
     1501 gaaaaacggg cggtaggatt tggcgcgttc tttctcggtt tccttggcgc agccggatca
     1561 acgatggggg ccgcctccat caccctgacg gttcaggcgc gccagctttt gagtgggatt
     1621 gtgcagcagc agaataacct ccttagggcc atagaggctc agcagcacct gctgaagctt
     1681 accgtttggg gcattaagca actgcaggcg cgggtactcg cggttgaaag ataccttaag
     1741 gatcagcaac tgctgggaat gtggggatgt agtggaaagt tcatctgcac aaccacggtc
     1801 ccatggaatt cctcatggtc aaataaatca cagtcagaaa tatgggataa tatgacgtgg
     1861 atgcagtggg acaaggagat taacaattac acccagatta tctacgatct tattgaggag
     1921 tcacaaaggc aacaagagaa aaacgaacag gacttgctgg cgctcgacaa atgggctaat
     1981 ttgtggaact ggttcgacat cagtaactgg ctgtggtaca taaagatttt cataatgata
     2041 gttggcgggc tcatagggct tcggattgcg ttcgcagtgc tttctgtcat caatagggtc
     2101 aggcaaggtt attccccgct ctcctttcaa acccataccc caaaccctcg ggatttggat
     2161 aggccaggcc ggatagagga ggaaggaggt gagcaggatc gggaccgaag cataagactg
     2221 gtctctgggt ttcttgcttt ggcatgggac gacctgaggt ctctgtgttt gttcagttac
     2281 caccgcctgc gagatttcat cttggtggct gctagaacgg tcgaacttct tggacatatt
     2341 tcacttaaag gcttgagacg aggctgggaa ggattgaaat accttgggaa ccttctgtca
     2401 tactggggac gggaactgaa gataagtgcc ataaacttgt tggacacaat cgcgattgtt
     2461 gtggcggaat ggaccgatcg gataattgag attggccaga gactgtgtcg cgctatcatt
     2521 aatatacctc ggagaatccg acaggggttc gaacgggctt tgttgtagta actcgaggcc
     2581 ggcaaggccg gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag
     2641 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     2701 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     2761 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc
     2821 tgattatgat ccggctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     2881 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     2941 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaggtc gactctagag
     3001 gatcgatccc cgccccggac gaactaaacc tgactacgac atctctgccc cttcttcgcg
     3061 gggcagtgca tgtaatccct tcagttggtt ggtacaactt gccaactggg ccctgttcca
     3121 catgtgacac ggggggggac caaacacaaa ggggttctct gactgtagtt gacatcctta
     3181 taaatggatg tgcacatttg ccaacactga gtggctttca tcctggagca gactttgcag
     3241 tctgtggact gcaacacaac attgccttta tgtgtaactc ttggctgaag ctcttacacc
     3301 aatgctgggg gacatgtacc tcccaggggc ccaggaagac tacgggaggc tacaccaacg
     3361 tcaatcagag gggcctgtgt agctaccgat aagcggaccc tcaagagggc attagcaata
     3421 gtgtttataa ggcccccttg ttaaccctaa acgggtagca tatgcttccc gggtagtagt
     3481 atatactatc cagactaacc ctaattcaat agcatatgtt acccaacggg aagcatatgc
     3541 tatcgaatta gggttagtaa aagggtccta aggaacagcg atatctccca ccccatgagc
     3601 tgtcacggtt ttatttacat ggggtcagga ttccacgagg gtagtgaacc attttagtca
     3661 caagggcagt ggctgaagat caaggagcgg gcagtgaact ctcctgaatc ttcgcctgct
     3721 tcttcattct ccttcgttta gctaatagaa taactgctga gttgtgaaca gtaaggtgta
     3781 tgtgaggtgc tcgaaaacaa ggtttcaggt gacgccccca gaataaaatt tggacggggg
     3841 gttcagtggt ggcattgtgc tatgacacca atataaccct cacaaacccc ttgggcaata
     3901 aatactagtg taggaatgaa acattctgaa tatctttaac aatagaaatc catggggtgg
     3961 ggacaagccg taaagactgg atgtccatct cacacgaatt tatggctatg ggcaacacat
     4021 aatcctagtg caatatgata ctggggttat taagatgtgt cccaggcagg gaccaagaca
     4081 ggtgaaccat gttgttacac tctatttgta acaaggggaa agagagtgga cgccgacagc
     4141 agcggactcc actggttgtc tctaacaccc ccgaaaatta aacggggctc cacgccaatg
     4201 gggcccataa acaaagacaa gtggccactc ttttttttga aattgtggag tgggggcacg
     4261 cgtcagcccc cacacgccgc cctgcggttt tggactgtaa aataagggtg taataacttg
     4321 gctgattgta accccgctaa ccactgcggt caaaccactt gcccacaaaa ccactaatgg
     4381 caccccgggg aatacctgca taagtaggtg ggcgggccaa gataggggcg cgattgctgc
     4441 gatctggagg acaaattaca cacacttgcg cctgagcgcc aagcacaggg ttgttggtcc
     4501 tcatattcac gaggtcgctg agagcacggt gggctaatgt tgccatgggt agcatatact
     4561 acccaaatat ctggatagca tatgctatcc taatctatat ctgggtagca taggctatcc
     4621 taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc
     4681 taatttatat ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc
     4741 taatctatat ctgggtagta tatgctatcc taatctgtat ccgggtagca tatgctatcc
     4801 taatagagat tagggtagta tatgctatcc taatttatat ctgggtagca tatactaccc
     4861 aaatatctgg atagcatatg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     4921 ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     4981 ctatatctgg gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat
     5041 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     5101 ctgtatccgg gtagcatatg ctatcctcat gcatatacag tcagcatatg atacccagta
     5161 gtagagtggg agtgctatcc tttgcatatg ccgccacctc ccaagggggc gtgaattttc
     5221 gctgcttgtc cttttcctgc tggttgctcc cattcttagg tgaatttaag gaggccaggc
     5281 taaagccgtc gcatgtctga ttgctcacca ggtaaatgtc gctaatgttt tccaacgcga
     5341 gaaggtgttg agcgcggagc tgagtgacgt gacaacatgg gtatgcccaa ttgccccatg
     5401 ttgggaggac gaaaatggtg acaagacaga tggccagaaa tacaccaaca gcacgcatga
     5461 tgtctactgg ggatttattc tttagtgcgg gggaatacac ggcttttaat acgattgagg
     5521 gcgtctccta acaagttaca tcactcctgc ccttcctcac cctcatctcc atcacctcct
     5581 tcatctccgt catctccgtc atcaccctcc gcggcagccc cttccaccat aggtggaaac
     5641 cagggaggca aatctactcc atcgtcaaag ctgcacacag tcaccctgat attgcaggta
     5701 ggagcgggct ttgtcataac aaggtcctta atcgcatcct tcaaaacctc agcaaatata
     5761 tgagtttgta aaaagaccat gaaataacag acaatggact cccttagcgg gccaggttgt
     5821 gggccgggtc caggggccat tccaaagggg agacgactca atggtgtaag acgacattgt
     5881 ggaatagcaa gggcagttcc tcgccttagg ttgtaaaggg aggtcttact acctccatat
     5941 acgaacacac cggcgaccca agttccttcg tcggtagtcc tttctacgtg actcctagcc
     6001 aggagagctc ttaaaccttc tgcaatgttc tcaaatttcg ggttggaacc tccttgacca
     6061 cgatgctttc caaaccaccc tccttttttg cgcctgcctc catcaccctg accccggggt
     6121 ccagtgcttg ggccttctcc tgggtcatct gcggggccct gctctatcgc tcccgggggc
     6181 acgtcaggct caccatctgg gccaccttct tggtggtatt caaaataatc ggcttcccct
     6241 acagggtgga aaaatggcct tctacctgga gggggcctgc gcggtggaga cccggatgat
     6301 gatgactgac tactgggact cctgggcctc ttttctccac gtccacgacc tctccccctg
     6361 gctctttcac gacttccccc cctggctctt tcacgtcctc taccccggcg gcctccacta
     6421 cctcctcgac cccggcctcc actacctcct cgaccccggc ctccactgcc tcctcgaccc
     6481 cggcctccac ctcctgctcc tgcccctcct gctcctgccc ctcctcctgc tcctgcccct
     6541 cctgcccctc ctgctcctgc ccctcctgcc cctcctgctc ctgcccctcc tgcccctcct
     6601 gctcctgccc ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc tcctgctcct
     6661 gcccctcctg cccctcctgc tcctgcccct cctgcccctc ctgctcctgc ccctcctgcc
     6721 cctcctgctc ctgcccctcc tgctcctgcc cctcctgctc ctgcccctcc tgctcctgcc
     6781 cctcctgccc ctcctgcccc tcctcctgct cctgcccctc ctgctcctgc ccctcctgcc
     6841 cctcctgccc ctcctgctcc tgcccctcct cctgctcctg cccctcctgc ccctcctgcc
     6901 cctcctcctg ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tcctgctcct
     6961 gcccctcctg cccctcctgc ccctcctcct gctcctgccc ctcctgcccc tcctcctgct
     7021 cctgcccctc ctcctgctcc tgcccctcct gcccctcctg cccctcctcc tgctcctgcc
     7081 cctcctcctg ctcctgcccc tcctgcccct cctgcccctc ctgcccctcc tcctgctcct
     7141 gcccctcctc ctgctcctgc ccctcctgct cctgcccctc ccgctcctgc tcctgctcct
     7201 gttccaccgt gggtcccttt gcagccaatg caacttggac gtttttgggg tctccggaca
     7261 ccatctctat gtcttggccc tgatcctgag ccgcccgggg ctcctggtct tccgcctcct
     7321 cgtcctcgtc ctcttccccg tcctcgtcca tggttatcac cccctcttct ttgaggtcca
     7381 ctgccgccgg agccttctgg tccagatgtg tctcccttct ctcctaggcc atttccaggt
     7441 cctgtacctg gcccctcgtc agacatgatt cacactaaaa gagatcaata gacatcttta
     7501 ttagacgacg ctcagtgaat acagggagtg cagactcctg ccccctccaa cagccccccc
     7561 accctcatcc ccttcatggt cgctgtcaga cagatccagg tctgaaaatt ccccatcctc
     7621 cgaaccatcc tcgtcctcat caccaattac tcgcagcccg gaaaactccc gctgaacatc
     7681 ctcaagattt gcgtcctgag cctcaagcca ggcctcaaat tcctcgtccc cctttttgct
     7741 ggacggtagg gatggggatt ctcgggaccc ctcctcttcc tcttcaaggt caccagacag
     7801 agatgctact ggggcaacgg aagaaaagct gggtgcggcc tgtgaggatc agcttatcga
     7861 tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt
     7921 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     7981 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     8041 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     8101 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     8161 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     8221 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     8281 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
     8341 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     8401 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     8461 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     8521 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     8581 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca
     8641 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     8701 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     8761 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     8821 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     8881 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     8941 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     9001 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     9061 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     9121 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     9181 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     9241 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     9301 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     9361 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     9421 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     9481 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     9541 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     9601 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     9661 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     9721 aacgcggcct ttttacggtt cctggccttt tgctggcctt gaagctgtcc ctgatggtcg
     9781 tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg
     9841 agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag
     9901 cccagcgcgt cggccccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg
     9961 atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc
    10021 caattcttgg agtggtgaat ccgttagcga ggtgccgccc tgcttcatcc ccgtggcccg
    10081 ttgctcgcgt ttgctggcgg tgtccccgga agaaatatat ttgcatgtct ttagttctat
    10141 gatgacacaa accccgccca gcgtcttgtc attggcgaat tcgaacacgc agatgcagtc
    10201 ggggcggcgc ggtccgaggt ccacttcgca tattaaggtg acgcgtgtgg cctcgaacac
    10261 cgagcgaccc tgcagcgacc cgcttaacag cgtcaacagc gtgccgcaga tcccgggggg
    10321 caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa
    10381 aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc
    10441 agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc
    10501 tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg
    10561 cttgacattg gggaattcag cgagagcctg acctattgca tctcccgccg tgcacagggt
    10621 gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag
    10681 gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga
    10741 ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc
    10801 catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct
    10861 ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg
    10921 gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg
    10981 agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg
    11041 tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca
    11101 ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc
    11161 ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc
    11221 cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg
    11281 accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg
    11341 gatcgggaga tgggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg
    11401 cgctatgacg gcaataaaaa gacagaataa aacgcacggg tgttgggtcg tttgttcata
    11461 aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg
    11521 gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc
    11581 cagggctcgc agccaacgtc ggggcggcag gccctgccat agccactggc cccgtgggtt
    11641 agggacgggg tcccccatgg ggaatggttt atggttcgtg ggggttatta ttttgggcgt
    11701 tgcgtggggt caggtccacg actggactga gcagacagac ccatggtttt tggatggcct
    11761 gggcatggac cgcatgtact ggcgcgacac gaacaccggg cgtctgtggc tgccaaacac
    11821 ccccgacccc caaaaaccac cgcgcggatt tctggcgtgc caagctagtc gaccaattct
    11881 catgtttgac agcttatcat cgcagatccg ggcaacgttg ttgccattgc tgcaggcgca
    11941 gaactggtag gtatggaaga tctatacatt gaatcaatat tggcaattag ccatattagt
    12001 cattggttat atagcataaa tcaatattgg ctattggcca ttgcatacgt tgtatctata
    12061 tcataatatg tacatttata ttggctcatg tccaatatga ccgccatgtt gacattgatt
    12121 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
    12181 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
    12241 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
    12301 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
    12361 tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
    12421 ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat tagtcatcgc
    12481 tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc
    12541 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
    12601 tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag
    12661 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc agatctctag
    12721 aagctgggta ccagctgcta gc
//
