LOCUS       sequence-235179-       12394 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the Q842.d12 HIV-1 
            isolate; C-terminal truncation; QES mutant with enhanced binding to 
            PG16.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12394)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 12394)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-Q842-QES.i04.c05-754*
FEATURES             Location/Qualifiers
     source          1..12394
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    2243..2377
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2267..2286)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2321..2340
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2520..2542)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      3102..4891
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(5193..7118)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(7553..7571)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7639..7743
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7744..7812,7813..8604)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7962..7981)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8775..9363
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9264..9283
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        9794..9939
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             9982..11019
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(11017..11036)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11061..11109
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        11763..12142
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12143..12346
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     12297..12317
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     12343..12367
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gccaccatgc caatgggatc actgcagccc ttggcaacgc tgtacttgct tggtatgctt
       61 gtggcatctg ttcttgctgt cgataatttg tgggtcacag tgtactacgg agtacctgta
      121 tggaaagagg cggaaactac tcttttttgc gcgtcagacg caaaggcata tgagactgaa
      181 aagcataatg tatgggctac ccatgcgtgc gtgcccacag accccaaccc ccaagaaatc
      241 catctggaga acgttactga agaatttaat atgtggaaga ataatatggt ggaacagatg
      301 catacggaca ttatcagtct gtgggatcag tctctgaaac catgtgttaa actcaccgat
      361 ctctgcgtaa ccttggactg taataacgtg acgaataatg gaactagtga catgagggaa
      421 gagataaaga attgctcctt caatatgaca acagaattgc gagacaagcg acaaaaagta
      481 tattctctgt tctacaaatt ggacctggtg cagataaatg aggatcaagg taacagctcc
      541 aataacaagt atcgcctgat aacttgcaac acgtctgcga ttacccaagc atgtcctaag
      601 atgacatttg aacccattcc gattcattat tgcgcacctg caggctatgc tatcctgaag
      661 tgcaaagatg aagagtttaa cgggatcggc ccgtgcaaaa acgtatccac tgtccaatgc
      721 acccacggta ttaagcctgt tgtgtccaca cagctccttc tcaatgggtc tctggcagag
      781 aaggaggtca aaataagatg tgaaaatata acgaacaacg caaaacctat cattgtccaa
      841 ctggtgaacc cagttaaaat caattgcacc cgacctaata ataatacgag aaagagtatc
      901 cacatcggcc cagggcaggc cttctacgct acaggcgata taatcggaga cataagacag
      961 gcgcactgca atgtaaatcg tacggaatgg aataatactc tccaccaggt agttgagcag
     1021 ttgcgaaagc attttaataa aacgattaac tttgctaact caaccggagg ggacttggaa
     1081 ataacaacac actcattcaa ttgcgggggc gaatggtttt attgtaacac gactaacctg
     1141 tttaatagca cttggaacca tacggctagt atgaactcaa ccgaatccaa tgacacaatc
     1201 atcctgccct gtcgaataaa acaaatcatc aatatgtggc aaagagtggg ccaagcaatg
     1261 tatgcacccc caataagagg tgtcataagg tgtgaatcca atataactgg gttgattctt
     1321 actagggatg gaggaaacac gaacagcacc cgagaaactt tccgcccagg cgggggtgat
     1381 atgagggata attggcgatc agaactttac aagtataagg tagttaagat agaacccctc
     1441 ggcgttgctc ccacaaaagc caagagaagg gttgtagaac gcgaaaagcg ggccgtaggg
     1501 attggcgctg tattcatcgg atttctcgga gcagctggat ctactatggg tgcagcttca
     1561 ataacattga cagttcaagc ccggcaactc ctttcaggta ttgtacagca gcaaagtaac
     1621 ctcctgcaag cgattgaggc tcagcagcat ctcctgaagc ttacggtgtg gggtatcaaa
     1681 cagcttcaag cgcgggtact cgcggttgag cgatacctta aggaccaaca attgcttgga
     1741 atgtggggct gcagcggtaa acttatatgt acaacatctg tgccgtggaa cagttcctgg
     1801 agtaacaaat cacaaaatga gatttgggac aatatgacct ggttgcagtg ggataaagaa
     1861 atatcaaact atacccagat catttacgat ctcttggagg agtctcaaaa ccagcaggag
     1921 aaaaacgaac aggacctcct cgcattggat aaatgggcca atttgtggaa ctggtttgac
     1981 atatctaact ggctttggta tataaagata ttcatcatga ttgtaggggg tcttattggt
     2041 ctgaggatag ttttcgcagt cttgtcagtc attaaccgag tgcggcaggg ttattctccc
     2101 ttgtcatttc aaactcacac tcctaatccg aggggactgg ataggcctga gcgaatagag
     2161 gaagagggcg gtgagcaaga caagaaccgg tcaatcaggc tcgtgtctgg tttcctgtga
     2221 taactcgagg ccggcaaggc cggatccaga catgataaga tacattgatg agtttggaca
     2281 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     2341 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     2401 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa
     2461 atgtggtatg gctgattatg atccggctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     2521 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     2581 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgagg
     2641 tcgactctag aggatcgatc cccgccccgg acgaactaaa cctgactacg acatctctgc
     2701 cccttcttcg cggggcagtg catgtaatcc cttcagttgg ttggtacaac ttgccaactg
     2761 ggccctgttc cacatgtgac acgggggggg accaaacaca aaggggttct ctgactgtag
     2821 ttgacatcct tataaatgga tgtgcacatt tgccaacact gagtggcttt catcctggag
     2881 cagactttgc agtctgtgga ctgcaacaca acattgcctt tatgtgtaac tcttggctga
     2941 agctcttaca ccaatgctgg gggacatgta cctcccaggg gcccaggaag actacgggag
     3001 gctacaccaa cgtcaatcag aggggcctgt gtagctaccg ataagcggac cctcaagagg
     3061 gcattagcaa tagtgtttat aaggccccct tgttaaccct aaacgggtag catatgcttc
     3121 ccgggtagta gtatatacta tccagactaa ccctaattca atagcatatg ttacccaacg
     3181 ggaagcatat gctatcgaat tagggttagt aaaagggtcc taaggaacag cgatatctcc
     3241 caccccatga gctgtcacgg ttttatttac atggggtcag gattccacga gggtagtgaa
     3301 ccattttagt cacaagggca gtggctgaag atcaaggagc gggcagtgaa ctctcctgaa
     3361 tcttcgcctg cttcttcatt ctccttcgtt tagctaatag aataactgct gagttgtgaa
     3421 cagtaaggtg tatgtgaggt gctcgaaaac aaggtttcag gtgacgcccc cagaataaaa
     3481 tttggacggg gggttcagtg gtggcattgt gctatgacac caatataacc ctcacaaacc
     3541 ccttgggcaa taaatactag tgtaggaatg aaacattctg aatatcttta acaatagaaa
     3601 tccatggggt ggggacaagc cgtaaagact ggatgtccat ctcacacgaa tttatggcta
     3661 tgggcaacac ataatcctag tgcaatatga tactggggtt attaagatgt gtcccaggca
     3721 gggaccaaga caggtgaacc atgttgttac actctatttg taacaagggg aaagagagtg
     3781 gacgccgaca gcagcggact ccactggttg tctctaacac ccccgaaaat taaacggggc
     3841 tccacgccaa tggggcccat aaacaaagac aagtggccac tctttttttt gaaattgtgg
     3901 agtgggggca cgcgtcagcc cccacacgcc gccctgcggt tttggactgt aaaataaggg
     3961 tgtaataact tggctgattg taaccccgct aaccactgcg gtcaaaccac ttgcccacaa
     4021 aaccactaat ggcaccccgg ggaatacctg cataagtagg tgggcgggcc aagatagggg
     4081 cgcgattgct gcgatctgga ggacaaatta cacacacttg cgcctgagcg ccaagcacag
     4141 ggttgttggt cctcatattc acgaggtcgc tgagagcacg gtgggctaat gttgccatgg
     4201 gtagcatata ctacccaaat atctggatag catatgctat cctaatctat atctgggtag
     4261 cataggctat cctaatctat atctgggtag catatgctat cctaatctat atctgggtag
     4321 tatatgctat cctaatttat atctgggtag cataggctat cctaatctat atctgggtag
     4381 catatgctat cctaatctat atctgggtag tatatgctat cctaatctgt atccgggtag
     4441 catatgctat cctaatagag attagggtag tatatgctat cctaatttat atctgggtag
     4501 catatactac ccaaatatct ggatagcata tgctatccta atctatatct gggtagcata
     4561 tgctatccta atctatatct gggtagcata ggctatccta atctatatct gggtagcata
     4621 tgctatccta atctatatct gggtagtata tgctatccta atttatatct gggtagcata
     4681 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     4741 tgctatccta atctgtatcc gggtagcata tgctatcctc atgcatatac agtcagcata
     4801 tgatacccag tagtagagtg ggagtgctat cctttgcata tgccgccacc tcccaagggg
     4861 gcgtgaattt tcgctgcttg tccttttcct gctggttgct cccattctta ggtgaattta
     4921 aggaggccag gctaaagccg tcgcatgtct gattgctcac caggtaaatg tcgctaatgt
     4981 tttccaacgc gagaaggtgt tgagcgcgga gctgagtgac gtgacaacat gggtatgccc
     5041 aattgcccca tgttgggagg acgaaaatgg tgacaagaca gatggccaga aatacaccaa
     5101 cagcacgcat gatgtctact ggggatttat tctttagtgc gggggaatac acggctttta
     5161 atacgattga gggcgtctcc taacaagtta catcactcct gcccttcctc accctcatct
     5221 ccatcacctc cttcatctcc gtcatctccg tcatcaccct ccgcggcagc cccttccacc
     5281 ataggtggaa accagggagg caaatctact ccatcgtcaa agctgcacac agtcaccctg
     5341 atattgcagg taggagcggg ctttgtcata acaaggtcct taatcgcatc cttcaaaacc
     5401 tcagcaaata tatgagtttg taaaaagacc atgaaataac agacaatgga ctcccttagc
     5461 gggccaggtt gtgggccggg tccaggggcc attccaaagg ggagacgact caatggtgta
     5521 agacgacatt gtggaatagc aagggcagtt cctcgcctta ggttgtaaag ggaggtctta
     5581 ctacctccat atacgaacac accggcgacc caagttcctt cgtcggtagt cctttctacg
     5641 tgactcctag ccaggagagc tcttaaacct tctgcaatgt tctcaaattt cgggttggaa
     5701 cctccttgac cacgatgctt tccaaaccac cctccttttt tgcgcctgcc tccatcaccc
     5761 tgaccccggg gtccagtgct tgggccttct cctgggtcat ctgcggggcc ctgctctatc
     5821 gctcccgggg gcacgtcagg ctcaccatct gggccacctt cttggtggta ttcaaaataa
     5881 tcggcttccc ctacagggtg gaaaaatggc cttctacctg gagggggcct gcgcggtgga
     5941 gacccggatg atgatgactg actactggga ctcctgggcc tcttttctcc acgtccacga
     6001 cctctccccc tggctctttc acgacttccc cccctggctc tttcacgtcc tctaccccgg
     6061 cggcctccac tacctcctcg accccggcct ccactacctc ctcgaccccg gcctccactg
     6121 cctcctcgac cccggcctcc acctcctgct cctgcccctc ctgctcctgc ccctcctcct
     6181 gctcctgccc ctcctgcccc tcctgctcct gcccctcctg cccctcctgc tcctgcccct
     6241 cctgcccctc ctgctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctgcccct
     6301 cctcctgctc ctgcccctcc tgcccctcct gctcctgccc ctcctgcccc tcctgctcct
     6361 gcccctcctg cccctcctgc tcctgcccct cctgctcctg cccctcctgc tcctgcccct
     6421 cctgctcctg cccctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctgctcct
     6481 gcccctcctg cccctcctgc ccctcctgct cctgcccctc ctcctgctcc tgcccctcct
     6541 gcccctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctcctgc tcctgcccct
     6601 cctcctgctc ctgcccctcc tgcccctcct gcccctcctc ctgctcctgc ccctcctgcc
     6661 cctcctcctg ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc tgcccctcct
     6721 cctgctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctgcccc tcctgcccct
     6781 cctcctgctc ctgcccctcc tcctgctcct gcccctcctg ctcctgcccc tcccgctcct
     6841 gctcctgctc ctgttccacc gtgggtccct ttgcagccaa tgcaacttgg acgtttttgg
     6901 ggtctccgga caccatctct atgtcttggc cctgatcctg agccgcccgg ggctcctggt
     6961 cttccgcctc ctcgtcctcg tcctcttccc cgtcctcgtc catggttatc accccctctt
     7021 ctttgaggtc cactgccgcc ggagccttct ggtccagatg tgtctccctt ctctcctagg
     7081 ccatttccag gtcctgtacc tggcccctcg tcagacatga ttcacactaa aagagatcaa
     7141 tagacatctt tattagacga cgctcagtga atacagggag tgcagactcc tgccccctcc
     7201 aacagccccc ccaccctcat ccccttcatg gtcgctgtca gacagatcca ggtctgaaaa
     7261 ttccccatcc tccgaaccat cctcgtcctc atcaccaatt actcgcagcc cggaaaactc
     7321 ccgctgaaca tcctcaagat ttgcgtcctg agcctcaagc caggcctcaa attcctcgtc
     7381 cccctttttg ctggacggta gggatgggga ttctcgggac ccctcctctt cctcttcaag
     7441 gtcaccagac agagatgcta ctggggcaac ggaagaaaag ctgggtgcgg cctgtgagga
     7501 tcagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg
     7561 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     7621 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     7681 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     7741 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     7801 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     7861 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     7921 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     7981 tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     8041 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     8101 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     8161 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     8221 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     8281 atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     8341 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     8401 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     8461 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     8521 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     8581 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     8641 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     8701 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     8761 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     8821 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     8881 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     8941 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     9001 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     9061 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     9121 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     9181 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     9241 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     9301 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     9361 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttgaagctgt
     9421 ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg
     9481 ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc
     9541 agcaagacgt agcccagcgc gtcggccccg agatgcgccg cgtgcggctg ctggagatgg
     9601 cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga
     9661 attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cctgcttcat
     9721 ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg gaagaaatat atttgcatgt
     9781 ctttagttct atgatgacac aaaccccgcc cagcgtcttg tcattggcga attcgaacac
     9841 gcagatgcag tcggggcggc gcggtccgag gtccacttcg catattaagg tgacgcgtgt
     9901 ggcctcgaac accgagcgac cctgcagcga cccgcttaac agcgtcaaca gcgtgccgca
     9961 gatcccgggg ggcaatgaga tatgaaaaag cctgaactca ccgcgacgtc tgtcgagaag
    10021 tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa
    10081 tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc
    10141 gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg
    10201 attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg catctcccgc
    10261 cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctgcag
    10321 ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc
    10381 ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg
    10441 attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc
    10501 gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac
    10561 ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg
    10621 gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc
    10681 ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat
    10741 ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa
    10801 ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc
    10861 gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc
    10921 gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc
    10981 agcactcgtc cggatcggga gatgggggag gctaactgaa acacggaagg agacaatacc
    11041 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg ggtgttgggt
    11101 cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga
    11161 gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt
    11221 cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc atagccactg
    11281 gccccgtggg ttagggacgg ggtcccccat ggggaatggt ttatggttcg tgggggttat
    11341 tattttgggc gttgcgtggg gtcaggtcca cgactggact gagcagacag acccatggtt
    11401 tttggatggc ctgggcatgg accgcatgta ctggcgcgac acgaacaccg ggcgtctgtg
    11461 gctgccaaac acccccgacc cccaaaaacc accgcgcgga tttctggcgt gccaagctag
    11521 tcgaccaatt ctcatgtttg acagcttatc atcgcagatc cgggcaacgt tgttgccatt
    11581 gctgcaggcg cagaactggt aggtatggaa gatctataca ttgaatcaat attggcaatt
    11641 agccatatta gtcattggtt atatagcata aatcaatatt ggctattggc cattgcatac
    11701 gttgtatcta tatcataata tgtacattta tattggctca tgtccaatat gaccgccatg
    11761 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
    11821 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
    11881 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
    11941 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
    12001 tcaagtgtat catatgccaa gtccgccccc tattgacgtc aatgacggta aatggcccgc
    12061 ctggcattat gcccagtaca tgaccttacg ggactttcct acttggcagt acatctacgt
    12121 attagtcatc gctattacca tggtgatgcg gttttggcag tacaccaatg ggcgtggata
    12181 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
    12241 ttggcaccaa aatcaacggg actttccaaa atgtcgtaat aaccccgccc cgttgacgca
    12301 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg
    12361 tcagatctct agaagctggg taccagctgc tagc
//
