LOCUS       sequence-235181-       12724 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the Q842.d12 HIV-1 
            isolate; QES mutant with enhanced binding to PG16.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12724)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 12724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-Q842-QES.i04.c05
FEATURES             Location/Qualifiers
     source          1..12724
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    2573..2707
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2597..2616)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2651..2670
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2850..2872)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      3432..5221
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(5523..7448)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(7883..7901)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7969..8073
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8074..8142,8143..8934)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8292..8311)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9105..9693
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9594..9613
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        10124..10269
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             10312..11349
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(11347..11366)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11391..11439
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        12093..12472
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12473..12676
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     12627..12647
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     12673..12697
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gccaccatgc caatgggatc actgcagccc ttggcaacgc tgtacttgct tggtatgctt
       61 gtggcatctg ttcttgctgt cgataatttg tgggtcacag tgtactacgg agtacctgta
      121 tggaaagagg cggaaactac tcttttttgc gcgtcagacg caaaggcata tgagactgaa
      181 aagcataatg tatgggctac ccatgcgtgc gtgcccacag accccaaccc ccaagaaatc
      241 catctggaga acgttactga agaatttaat atgtggaaga ataatatggt ggaacagatg
      301 catacggaca ttatcagtct gtgggatcag tctctgaaac catgtgttaa actcaccgat
      361 ctctgcgtaa ccttggactg taataacgtg acgaataatg gaactagtga catgagggaa
      421 gagataaaga attgctcctt caatatgaca acagaattgc gagacaagcg acaaaaagta
      481 tattctctgt tctacaaatt ggacctggtg cagataaatg aggatcaagg taacagctcc
      541 aataacaagt atcgcctgat aacttgcaac acgtctgcga ttacccaagc atgtcctaag
      601 atgacatttg aacccattcc gattcattat tgcgcacctg caggctatgc tatcctgaag
      661 tgcaaagatg aagagtttaa cgggatcggc ccgtgcaaaa acgtatccac tgtccaatgc
      721 acccacggta ttaagcctgt tgtgtccaca cagctccttc tcaatgggtc tctggcagag
      781 aaggaggtca aaataagatg tgaaaatata acgaacaacg caaaacctat cattgtccaa
      841 ctggtgaacc cagttaaaat caattgcacc cgacctaata ataatacgag aaagagtatc
      901 cacatcggcc cagggcaggc cttctacgct acaggcgata taatcggaga cataagacag
      961 gcgcactgca atgtaaatcg tacggaatgg aataatactc tccaccaggt agttgagcag
     1021 ttgcgaaagc attttaataa aacgattaac tttgctaact caaccggagg ggacttggaa
     1081 ataacaacac actcattcaa ttgcgggggc gaatggtttt attgtaacac gactaacctg
     1141 tttaatagca cttggaacca tacggctagt atgaactcaa ccgaatccaa tgacacaatc
     1201 atcctgccct gtcgaataaa acaaatcatc aatatgtggc aaagagtggg ccaagcaatg
     1261 tatgcacccc caataagagg tgtcataagg tgtgaatcca atataactgg gttgattctt
     1321 actagggatg gaggaaacac gaacagcacc cgagaaactt tccgcccagg cgggggtgat
     1381 atgagggata attggcgatc agaactttac aagtataagg tagttaagat agaacccctc
     1441 ggcgttgctc ccacaaaagc caagagaagg gttgtagaac gcgaaaagcg ggccgtaggg
     1501 attggcgctg tattcatcgg atttctcgga gcagctggat ctactatggg tgcagcttca
     1561 ataacattga cagttcaagc ccggcaactc ctttcaggta ttgtacagca gcaaagtaac
     1621 ctcctgcaag cgattgaggc tcagcagcat ctcctgaagc ttacggtgtg gggtatcaaa
     1681 cagcttcaag cgcgggtact cgcggttgag cgatacctta aggaccaaca attgcttgga
     1741 atgtggggct gcagcggtaa acttatatgt acaacatctg tgccgtggaa cagttcctgg
     1801 agtaacaaat cacaaaatga gatttgggac aatatgacct ggttgcagtg ggataaagaa
     1861 atatcaaact atacccagat catttacgat ctcttggagg agtctcaaaa ccagcaggag
     1921 aaaaacgaac aggacctcct cgcattggat aaatgggcca atttgtggaa ctggtttgac
     1981 atatctaact ggctttggta tataaagata ttcatcatga ttgtaggggg tcttattggt
     2041 ctgaggatag ttttcgcagt cttgtcagtc attaaccgag tgcggcaggg ttattctccc
     2101 ttgtcatttc aaactcacac tcctaatccg aggggactgg ataggcctga gcgaatagag
     2161 gaagagggcg gtgagcaaga caagaaccgg tcaatcaggc tcgtgtctgg tttcctggca
     2221 ctggcgtggg atgatctgag gagtctttgt cttttctcat accatcggtt gcgcgacttc
     2281 atactcatcg tcgccaggac cgtagaactt ttgggacatt cctccttgaa gggacttcgg
     2341 ctcggatggg agggattgaa gtacttgggg aatctgctga gttattgggg gcgggaattg
     2401 cgaatctccg ccacaaatct cttggatacg attgctatag taatagctgg atggacggat
     2461 agagtaatcg aaatagggca gcgcctctgt cgagcatttc tcaatatccc cagaagaata
     2521 aggcaaggat ttgagcgcgc tctgctctag taactcgagg ccggcaaggc cggatccaga
     2581 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     2641 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     2701 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
     2761 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atccggctgc
     2821 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     2881 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     2941 gttggcgggt gtcggggcgc agccatgagg tcgactctag aggatcgatc cccgccccgg
     3001 acgaactaaa cctgactacg acatctctgc cccttcttcg cggggcagtg catgtaatcc
     3061 cttcagttgg ttggtacaac ttgccaactg ggccctgttc cacatgtgac acgggggggg
     3121 accaaacaca aaggggttct ctgactgtag ttgacatcct tataaatgga tgtgcacatt
     3181 tgccaacact gagtggcttt catcctggag cagactttgc agtctgtgga ctgcaacaca
     3241 acattgcctt tatgtgtaac tcttggctga agctcttaca ccaatgctgg gggacatgta
     3301 cctcccaggg gcccaggaag actacgggag gctacaccaa cgtcaatcag aggggcctgt
     3361 gtagctaccg ataagcggac cctcaagagg gcattagcaa tagtgtttat aaggccccct
     3421 tgttaaccct aaacgggtag catatgcttc ccgggtagta gtatatacta tccagactaa
     3481 ccctaattca atagcatatg ttacccaacg ggaagcatat gctatcgaat tagggttagt
     3541 aaaagggtcc taaggaacag cgatatctcc caccccatga gctgtcacgg ttttatttac
     3601 atggggtcag gattccacga gggtagtgaa ccattttagt cacaagggca gtggctgaag
     3661 atcaaggagc gggcagtgaa ctctcctgaa tcttcgcctg cttcttcatt ctccttcgtt
     3721 tagctaatag aataactgct gagttgtgaa cagtaaggtg tatgtgaggt gctcgaaaac
     3781 aaggtttcag gtgacgcccc cagaataaaa tttggacggg gggttcagtg gtggcattgt
     3841 gctatgacac caatataacc ctcacaaacc ccttgggcaa taaatactag tgtaggaatg
     3901 aaacattctg aatatcttta acaatagaaa tccatggggt ggggacaagc cgtaaagact
     3961 ggatgtccat ctcacacgaa tttatggcta tgggcaacac ataatcctag tgcaatatga
     4021 tactggggtt attaagatgt gtcccaggca gggaccaaga caggtgaacc atgttgttac
     4081 actctatttg taacaagggg aaagagagtg gacgccgaca gcagcggact ccactggttg
     4141 tctctaacac ccccgaaaat taaacggggc tccacgccaa tggggcccat aaacaaagac
     4201 aagtggccac tctttttttt gaaattgtgg agtgggggca cgcgtcagcc cccacacgcc
     4261 gccctgcggt tttggactgt aaaataaggg tgtaataact tggctgattg taaccccgct
     4321 aaccactgcg gtcaaaccac ttgcccacaa aaccactaat ggcaccccgg ggaatacctg
     4381 cataagtagg tgggcgggcc aagatagggg cgcgattgct gcgatctgga ggacaaatta
     4441 cacacacttg cgcctgagcg ccaagcacag ggttgttggt cctcatattc acgaggtcgc
     4501 tgagagcacg gtgggctaat gttgccatgg gtagcatata ctacccaaat atctggatag
     4561 catatgctat cctaatctat atctgggtag cataggctat cctaatctat atctgggtag
     4621 catatgctat cctaatctat atctgggtag tatatgctat cctaatttat atctgggtag
     4681 cataggctat cctaatctat atctgggtag catatgctat cctaatctat atctgggtag
     4741 tatatgctat cctaatctgt atccgggtag catatgctat cctaatagag attagggtag
     4801 tatatgctat cctaatttat atctgggtag catatactac ccaaatatct ggatagcata
     4861 tgctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagcata
     4921 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     4981 tgctatccta atttatatct gggtagcata ggctatccta atctatatct gggtagcata
     5041 tgctatccta atctatatct gggtagtata tgctatccta atctgtatcc gggtagcata
     5101 tgctatcctc atgcatatac agtcagcata tgatacccag tagtagagtg ggagtgctat
     5161 cctttgcata tgccgccacc tcccaagggg gcgtgaattt tcgctgcttg tccttttcct
     5221 gctggttgct cccattctta ggtgaattta aggaggccag gctaaagccg tcgcatgtct
     5281 gattgctcac caggtaaatg tcgctaatgt tttccaacgc gagaaggtgt tgagcgcgga
     5341 gctgagtgac gtgacaacat gggtatgccc aattgcccca tgttgggagg acgaaaatgg
     5401 tgacaagaca gatggccaga aatacaccaa cagcacgcat gatgtctact ggggatttat
     5461 tctttagtgc gggggaatac acggctttta atacgattga gggcgtctcc taacaagtta
     5521 catcactcct gcccttcctc accctcatct ccatcacctc cttcatctcc gtcatctccg
     5581 tcatcaccct ccgcggcagc cccttccacc ataggtggaa accagggagg caaatctact
     5641 ccatcgtcaa agctgcacac agtcaccctg atattgcagg taggagcggg ctttgtcata
     5701 acaaggtcct taatcgcatc cttcaaaacc tcagcaaata tatgagtttg taaaaagacc
     5761 atgaaataac agacaatgga ctcccttagc gggccaggtt gtgggccggg tccaggggcc
     5821 attccaaagg ggagacgact caatggtgta agacgacatt gtggaatagc aagggcagtt
     5881 cctcgcctta ggttgtaaag ggaggtctta ctacctccat atacgaacac accggcgacc
     5941 caagttcctt cgtcggtagt cctttctacg tgactcctag ccaggagagc tcttaaacct
     6001 tctgcaatgt tctcaaattt cgggttggaa cctccttgac cacgatgctt tccaaaccac
     6061 cctccttttt tgcgcctgcc tccatcaccc tgaccccggg gtccagtgct tgggccttct
     6121 cctgggtcat ctgcggggcc ctgctctatc gctcccgggg gcacgtcagg ctcaccatct
     6181 gggccacctt cttggtggta ttcaaaataa tcggcttccc ctacagggtg gaaaaatggc
     6241 cttctacctg gagggggcct gcgcggtgga gacccggatg atgatgactg actactggga
     6301 ctcctgggcc tcttttctcc acgtccacga cctctccccc tggctctttc acgacttccc
     6361 cccctggctc tttcacgtcc tctaccccgg cggcctccac tacctcctcg accccggcct
     6421 ccactacctc ctcgaccccg gcctccactg cctcctcgac cccggcctcc acctcctgct
     6481 cctgcccctc ctgctcctgc ccctcctcct gctcctgccc ctcctgcccc tcctgctcct
     6541 gcccctcctg cccctcctgc tcctgcccct cctgcccctc ctgctcctgc ccctcctgcc
     6601 cctcctcctg ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tgcccctcct
     6661 gctcctgccc ctcctgcccc tcctgctcct gcccctcctg cccctcctgc tcctgcccct
     6721 cctgctcctg cccctcctgc tcctgcccct cctgctcctg cccctcctgc ccctcctgcc
     6781 cctcctcctg ctcctgcccc tcctgctcct gcccctcctg cccctcctgc ccctcctgct
     6841 cctgcccctc ctcctgctcc tgcccctcct gcccctcctg cccctcctcc tgctcctgcc
     6901 cctcctgccc ctcctcctgc tcctgcccct cctcctgctc ctgcccctcc tgcccctcct
     6961 gcccctcctc ctgctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctcctgct
     7021 cctgcccctc ctgcccctcc tgcccctcct cctgctcctg cccctcctcc tgctcctgcc
     7081 cctcctgccc ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tcctgctcct
     7141 gcccctcctg ctcctgcccc tcccgctcct gctcctgctc ctgttccacc gtgggtccct
     7201 ttgcagccaa tgcaacttgg acgtttttgg ggtctccgga caccatctct atgtcttggc
     7261 cctgatcctg agccgcccgg ggctcctggt cttccgcctc ctcgtcctcg tcctcttccc
     7321 cgtcctcgtc catggttatc accccctctt ctttgaggtc cactgccgcc ggagccttct
     7381 ggtccagatg tgtctccctt ctctcctagg ccatttccag gtcctgtacc tggcccctcg
     7441 tcagacatga ttcacactaa aagagatcaa tagacatctt tattagacga cgctcagtga
     7501 atacagggag tgcagactcc tgccccctcc aacagccccc ccaccctcat ccccttcatg
     7561 gtcgctgtca gacagatcca ggtctgaaaa ttccccatcc tccgaaccat cctcgtcctc
     7621 atcaccaatt actcgcagcc cggaaaactc ccgctgaaca tcctcaagat ttgcgtcctg
     7681 agcctcaagc caggcctcaa attcctcgtc cccctttttg ctggacggta gggatgggga
     7741 ttctcgggac ccctcctctt cctcttcaag gtcaccagac agagatgcta ctggggcaac
     7801 ggaagaaaag ctgggtgcgg cctgtgagga tcagcttatc gatgataagc tgtcaaacat
     7861 gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     7921 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     7981 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     8041 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     8101 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     8161 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     8221 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     8281 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc
     8341 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     8401 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     8461 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     8521 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     8581 gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc
     8641 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     8701 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     8761 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     8821 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     8881 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     8941 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     9001 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     9061 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     9121 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     9181 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     9241 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     9301 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     9361 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     9421 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     9481 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     9541 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     9601 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     9661 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     9721 ttcctggcct tttgctggcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac
     9781 agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg
     9841 aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccccg
     9901 agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt
     9961 tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga
    10021 atccgttagc gaggtgccgc cctgcttcat ccccgtggcc cgttgctcgc gtttgctggc
    10081 ggtgtccccg gaagaaatat atttgcatgt ctttagttct atgatgacac aaaccccgcc
    10141 cagcgtcttg tcattggcga attcgaacac gcagatgcag tcggggcggc gcggtccgag
    10201 gtccacttcg catattaagg tgacgcgtgt ggcctcgaac accgagcgac cctgcagcga
    10261 cccgcttaac agcgtcaaca gcgtgccgca gatcccgggg ggcaatgaga tatgaaaaag
    10321 cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc
    10381 gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg
    10441 cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt
    10501 tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc
    10561 agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg
    10621 cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct
    10681 gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa
    10741 tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa
    10801 actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt
    10861 tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat
    10921 gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg
    10981 gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag
    11041 cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg
    11101 gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc
    11161 gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact
    11221 gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa
    11281 gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cggatcggga gatgggggag
    11341 gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa
    11401 aagacagaat aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg gttcggtccc
    11461 agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt
    11521 cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg
    11581 tcggggcggc aggccctgcc atagccactg gccccgtggg ttagggacgg ggtcccccat
    11641 ggggaatggt ttatggttcg tgggggttat tattttgggc gttgcgtggg gtcaggtcca
    11701 cgactggact gagcagacag acccatggtt tttggatggc ctgggcatgg accgcatgta
    11761 ctggcgcgac acgaacaccg ggcgtctgtg gctgccaaac acccccgacc cccaaaaacc
    11821 accgcgcgga tttctggcgt gccaagctag tcgaccaatt ctcatgtttg acagcttatc
    11881 atcgcagatc cgggcaacgt tgttgccatt gctgcaggcg cagaactggt aggtatggaa
    11941 gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt atatagcata
    12001 aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata tgtacattta
    12061 tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta gttattaata
    12121 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
    12181 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
    12241 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
    12301 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtccgccccc
    12361 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttacg
    12421 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
    12481 gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
    12541 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
    12601 atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt
    12661 ctatataagc agagctcgtt tagtgaaccg tcagatctct agaagctggg taccagctgc
    12721 tagc
//
