LOCUS       sequence-235182-       12715 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the 191084 B7-19 HIV-1 
            isolate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12715)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 12715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-191084-gp160
FEATURES             Location/Qualifiers
     source          1..12715
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    2564..2698
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2588..2607)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2642..2661
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2841..2863)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      3423..5212
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(5514..7439)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(7874..7892)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7960..8064
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8065..8133,8134..8925)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8283..8302)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9096..9684
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9585..9604
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        10115..10260
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             10303..11340
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(11338..11357)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11382..11430
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        12084..12463
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12464..12667
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     12618..12638
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     12664..12688
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gccaccatgc cgatggggtc tctccagccg ctggccaccc tctacctgtt gggtatgttg
       61 gtggcgtccg tgctggcgac agagaatctg tgggttacag tctactacgg agttccagtc
      121 tggagggacg cagagacaac acttttttgt gccagcgacg ctaaagcgta tgacactgaa
      181 atgcataatg tctgggcaac tcatgcctgt gttcccacag acccgaatcc acaagaaata
      241 gacttggaga atgtaacgga gaaatttaac atgtggaaaa ataacatggt tgaacaaatg
      301 catacggata taatcagcct ttgggaccaa tccctgaagc cgtgcgttaa gttgacaccc
      361 ttgtgtgtta ctctgaactg cacagccatt accaatgata cgagggggaa tgagactggt
      421 atcaatcgga ccgtcgagac gaccgaaatg accaactgtt cattcaatat gaccactgaa
      481 cttagggacc gaaaaaagaa ggtcaatgcg cttttttata agcttgacat cgttcagata
      541 ggagaaaatt catctagcca gtaccgcctc attaattgta ataccagtgt tattacacaa
      601 gcctgcccaa aagttacctt cgagccaatt ccaattcatt attgcgcgcc tgctggattt
      661 gccatcctta agtgcaagga caaagagttc aacgggacag gaacttgtag gaacgtgtcc
      721 agtgtgcaat gcactcacgg tataaaaccc gtcgtgagca cgcaactttt gctcaacggg
      781 tccctggcgg aaggacaggt gaagatccgg tcagagaaca tctcagacaa tgcaaaaacc
      841 atcatcgtcc agttgaatga gtctgttcca atcaattgta ctagaccaaa caacaataca
      901 cgaaggggga tccacctggg tccgggccaa acattctttg ctaccgacat aattggtgac
      961 attcgccaag ctcactgtaa tgttagcgaa tcaaaatgga acaaggcgct ccaggaagtt
     1021 gtgaaacagc tgaggcagca ctggaacaaa acgataatct tcaaatcctc atccggtggg
     1081 gatctcgaaa tcacgactca ctccttcaac tgtggaggtg agttcttcta ctgtaacacc
     1141 tcaggcctgt tcaatagcac atggaacata gccggcaaca gaacgaatga tacgaagtca
     1201 aatgaaacaa tcaccctccc gtgtcgaata aaacagatcg taaatgtttg gcaaagggta
     1261 ggacaagcga tctacgcgcc cccaattgca ggagtcattc ggtgcaattc aaacatcaca
     1321 ggcctcctct tggtaagaga tgggggagct actaataata cagacgaaac gttccgccct
     1381 ggaggcggga acatgcgcga taattggaga agtgagttgt ataaatataa ggtggtcaag
     1441 atagagcctc tgggcgttgc accgaccagg gcgcgcaggc gcgtggtgga aagggagaag
     1501 agggctgtag gactcggagc cgtatttatt ggatttcttg gggcagcggg ttctaccatg
     1561 ggtgcagctt ccgtgacctt gaccgtacaa gccaggcaac ttctttctgg aatcgttcaa
     1621 caacaaagca atcttctccg agcaatcgag gcccagcagc atctcttgaa acttacagtc
     1681 tggggcataa agcagctcca ggcgcgcgtg ctcgcagttg agagatattt gaaagaccaa
     1741 cagctgcttg gtatttgggg ctgttctgga aaattgatat gcccgactac cgtaccatgg
     1801 aacagtagct ggtcaaacaa atcacaaaac gaaatctggg ataatatgac ctggcttcaa
     1861 tgggacaagg agatttcaaa ttacacacag cttatatatt cattgattga ggaaagccaa
     1921 aatcagcagg agaagaacga gcaggagctg ttggcgctgg acaagtgggc atctttgtgg
     1981 aattggttcg acatttccaa gtggctttgg tatatcagaa tatttataat gatcgtgggc
     2041 gggttgatag gacttagaat tgtattcaca gttctcagca tcattaatag ggttcgccag
     2101 ggttactccc cgctctcttt tcagacacat ccgccatctc caacaggtcc tgataggcct
     2161 gggcggattg aagaagaggg tggtgagcaa ggacgggata gaagcaccag gctggtatct
     2221 ggattcttgg cattggcctg ggatgatctc agatccctct tcctctttag ttatcatcac
     2281 ctccgagatc tcatcctcat cgtcgcaagg gcggtcgagc ttctgggcca taatagtctt
     2341 aagggactta aacttggctg gaaccttctc ctgtactggt ctagagagtt gaagatttcc
     2401 gcgttgaatt tgttggatac cattgccatc gcagtggcgg gttggaccga ccgagttatc
     2461 gagatagggc agagaatttg ccgggcaata cggaatattc caagacgaat tagacagggc
     2521 gcggaaagag catttctgta gtaactcgag gccggcaagg ccggatccag acatgataag
     2581 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     2641 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     2701 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     2761 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatccggctg cctcgcgcgt
     2821 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     2881 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     2941 tgtcggggcg cagccatgag gtcgactcta gaggatcgat ccccgccccg gacgaactaa
     3001 acctgactac gacatctctg ccccttcttc gcggggcagt gcatgtaatc ccttcagttg
     3061 gttggtacaa cttgccaact gggccctgtt ccacatgtga cacggggggg gaccaaacac
     3121 aaaggggttc tctgactgta gttgacatcc ttataaatgg atgtgcacat ttgccaacac
     3181 tgagtggctt tcatcctgga gcagactttg cagtctgtgg actgcaacac aacattgcct
     3241 ttatgtgtaa ctcttggctg aagctcttac accaatgctg ggggacatgt acctcccagg
     3301 ggcccaggaa gactacggga ggctacacca acgtcaatca gaggggcctg tgtagctacc
     3361 gataagcgga ccctcaagag ggcattagca atagtgttta taaggccccc ttgttaaccc
     3421 taaacgggta gcatatgctt cccgggtagt agtatatact atccagacta accctaattc
     3481 aatagcatat gttacccaac gggaagcata tgctatcgaa ttagggttag taaaagggtc
     3541 ctaaggaaca gcgatatctc ccaccccatg agctgtcacg gttttattta catggggtca
     3601 ggattccacg agggtagtga accattttag tcacaagggc agtggctgaa gatcaaggag
     3661 cgggcagtga actctcctga atcttcgcct gcttcttcat tctccttcgt ttagctaata
     3721 gaataactgc tgagttgtga acagtaaggt gtatgtgagg tgctcgaaaa caaggtttca
     3781 ggtgacgccc ccagaataaa atttggacgg ggggttcagt ggtggcattg tgctatgaca
     3841 ccaatataac cctcacaaac cccttgggca ataaatacta gtgtaggaat gaaacattct
     3901 gaatatcttt aacaatagaa atccatgggg tggggacaag ccgtaaagac tggatgtcca
     3961 tctcacacga atttatggct atgggcaaca cataatccta gtgcaatatg atactggggt
     4021 tattaagatg tgtcccaggc agggaccaag acaggtgaac catgttgtta cactctattt
     4081 gtaacaaggg gaaagagagt ggacgccgac agcagcggac tccactggtt gtctctaaca
     4141 cccccgaaaa ttaaacgggg ctccacgcca atggggccca taaacaaaga caagtggcca
     4201 ctcttttttt tgaaattgtg gagtgggggc acgcgtcagc ccccacacgc cgccctgcgg
     4261 ttttggactg taaaataagg gtgtaataac ttggctgatt gtaaccccgc taaccactgc
     4321 ggtcaaacca cttgcccaca aaaccactaa tggcaccccg gggaatacct gcataagtag
     4381 gtgggcgggc caagataggg gcgcgattgc tgcgatctgg aggacaaatt acacacactt
     4441 gcgcctgagc gccaagcaca gggttgttgg tcctcatatt cacgaggtcg ctgagagcac
     4501 ggtgggctaa tgttgccatg ggtagcatat actacccaaa tatctggata gcatatgcta
     4561 tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta
     4621 tcctaatcta tatctgggta gtatatgcta tcctaattta tatctgggta gcataggcta
     4681 tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta
     4741 tcctaatctg tatccgggta gcatatgcta tcctaataga gattagggta gtatatgcta
     4801 tcctaattta tatctgggta gcatatacta cccaaatatc tggatagcat atgctatcct
     4861 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagcat aggctatcct
     4921 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct
     4981 aatttatatc tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct
     5041 aatctatatc tgggtagtat atgctatcct aatctgtatc cgggtagcat atgctatcct
     5101 catgcatata cagtcagcat atgataccca gtagtagagt gggagtgcta tcctttgcat
     5161 atgccgccac ctcccaaggg ggcgtgaatt ttcgctgctt gtccttttcc tgctggttgc
     5221 tcccattctt aggtgaattt aaggaggcca ggctaaagcc gtcgcatgtc tgattgctca
     5281 ccaggtaaat gtcgctaatg ttttccaacg cgagaaggtg ttgagcgcgg agctgagtga
     5341 cgtgacaaca tgggtatgcc caattgcccc atgttgggag gacgaaaatg gtgacaagac
     5401 agatggccag aaatacacca acagcacgca tgatgtctac tggggattta ttctttagtg
     5461 cgggggaata cacggctttt aatacgattg agggcgtctc ctaacaagtt acatcactcc
     5521 tgcccttcct caccctcatc tccatcacct ccttcatctc cgtcatctcc gtcatcaccc
     5581 tccgcggcag ccccttccac cataggtgga aaccagggag gcaaatctac tccatcgtca
     5641 aagctgcaca cagtcaccct gatattgcag gtaggagcgg gctttgtcat aacaaggtcc
     5701 ttaatcgcat ccttcaaaac ctcagcaaat atatgagttt gtaaaaagac catgaaataa
     5761 cagacaatgg actcccttag cgggccaggt tgtgggccgg gtccaggggc cattccaaag
     5821 gggagacgac tcaatggtgt aagacgacat tgtggaatag caagggcagt tcctcgcctt
     5881 aggttgtaaa gggaggtctt actacctcca tatacgaaca caccggcgac ccaagttcct
     5941 tcgtcggtag tcctttctac gtgactccta gccaggagag ctcttaaacc ttctgcaatg
     6001 ttctcaaatt tcgggttgga acctccttga ccacgatgct ttccaaacca ccctcctttt
     6061 ttgcgcctgc ctccatcacc ctgaccccgg ggtccagtgc ttgggccttc tcctgggtca
     6121 tctgcggggc cctgctctat cgctcccggg ggcacgtcag gctcaccatc tgggccacct
     6181 tcttggtggt attcaaaata atcggcttcc cctacagggt ggaaaaatgg ccttctacct
     6241 ggagggggcc tgcgcggtgg agacccggat gatgatgact gactactggg actcctgggc
     6301 ctcttttctc cacgtccacg acctctcccc ctggctcttt cacgacttcc ccccctggct
     6361 ctttcacgtc ctctaccccg gcggcctcca ctacctcctc gaccccggcc tccactacct
     6421 cctcgacccc ggcctccact gcctcctcga ccccggcctc cacctcctgc tcctgcccct
     6481 cctgctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctgctcc tgcccctcct
     6541 gcccctcctg ctcctgcccc tcctgcccct cctgctcctg cccctcctgc ccctcctcct
     6601 gctcctgccc ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc tgctcctgcc
     6661 cctcctgccc ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc tcctgctcct
     6721 gcccctcctg ctcctgcccc tcctgctcct gcccctcctg cccctcctgc ccctcctcct
     6781 gctcctgccc ctcctgctcc tgcccctcct gcccctcctg cccctcctgc tcctgcccct
     6841 cctcctgctc ctgcccctcc tgcccctcct gcccctcctc ctgctcctgc ccctcctgcc
     6901 cctcctcctg ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc tgcccctcct
     6961 cctgctcctg cccctcctgc ccctcctcct gctcctgccc ctcctcctgc tcctgcccct
     7021 cctgcccctc ctgcccctcc tcctgctcct gcccctcctc ctgctcctgc ccctcctgcc
     7081 cctcctgccc ctcctgcccc tcctcctgct cctgcccctc ctcctgctcc tgcccctcct
     7141 gctcctgccc ctcccgctcc tgctcctgct cctgttccac cgtgggtccc tttgcagcca
     7201 atgcaacttg gacgtttttg gggtctccgg acaccatctc tatgtcttgg ccctgatcct
     7261 gagccgcccg gggctcctgg tcttccgcct cctcgtcctc gtcctcttcc ccgtcctcgt
     7321 ccatggttat caccccctct tctttgaggt ccactgccgc cggagccttc tggtccagat
     7381 gtgtctccct tctctcctag gccatttcca ggtcctgtac ctggcccctc gtcagacatg
     7441 attcacacta aaagagatca atagacatct ttattagacg acgctcagtg aatacaggga
     7501 gtgcagactc ctgccccctc caacagcccc cccaccctca tccccttcat ggtcgctgtc
     7561 agacagatcc aggtctgaaa attccccatc ctccgaacca tcctcgtcct catcaccaat
     7621 tactcgcagc ccggaaaact cccgctgaac atcctcaaga tttgcgtcct gagcctcaag
     7681 ccaggcctca aattcctcgt cccccttttt gctggacggt agggatgggg attctcggga
     7741 cccctcctct tcctcttcaa ggtcaccaga cagagatgct actggggcaa cggaagaaaa
     7801 gctgggtgcg gcctgtgagg atcagcttat cgatgataag ctgtcaaaca tgagaattct
     7861 tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     7921 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     7981 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     8041 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     8101 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     8161 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     8221 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     8281 ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc
     8341 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     8401 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     8461 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     8521 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     8581 aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta
     8641 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     8701 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     8761 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     8821 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     8881 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     8941 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     9001 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     9061 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     9121 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     9181 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     9241 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     9301 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     9361 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     9421 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     9481 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     9541 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     9601 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     9661 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     9721 ttttgctggc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc
     9781 tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc
     9841 cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggcccc gagatgcgcc
     9901 gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg
     9961 cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag
    10021 cgaggtgccg ccctgcttca tccccgtggc ccgttgctcg cgtttgctgg cggtgtcccc
    10081 ggaagaaata tatttgcatg tctttagttc tatgatgaca caaaccccgc ccagcgtctt
    10141 gtcattggcg aattcgaaca cgcagatgca gtcggggcgg cgcggtccga ggtccacttc
    10201 gcatattaag gtgacgcgtg tggcctcgaa caccgagcga ccctgcagcg acccgcttaa
    10261 cagcgtcaac agcgtgccgc agatcccggg gggcaatgag atatgaaaaa gcctgaactc
    10321 accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg
    10381 cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat
    10441 gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac
    10501 tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggaatt cagcgagagc
    10561 ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc
    10621 gaactgcccg ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat
    10681 cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca
    10741 tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg
    10801 gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag
    10861 gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg
    10921 gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa
    10981 tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg
    11041 cgctacttcg agcggaggca tccggagctt gcaggatcgc cgcggctccg ggcgtatatg
    11101 ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca
    11161 gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt
    11221 acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc
    11281 gatagtggaa accgacgccc cagcactcgt ccggatcggg agatggggga ggctaactga
    11341 aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa
    11401 taaaacgcac gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc
    11461 actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt
    11521 ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg
    11581 caggccctgc catagccact ggccccgtgg gttagggacg gggtccccca tggggaatgg
    11641 tttatggttc gtgggggtta ttattttggg cgttgcgtgg ggtcaggtcc acgactggac
    11701 tgagcagaca gacccatggt ttttggatgg cctgggcatg gaccgcatgt actggcgcga
    11761 cacgaacacc gggcgtctgt ggctgccaaa cacccccgac ccccaaaaac caccgcgcgg
    11821 atttctggcg tgccaagcta gtcgaccaat tctcatgttt gacagcttat catcgcagat
    11881 ccgggcaacg ttgttgccat tgctgcaggc gcagaactgg taggtatgga agatctatac
    11941 attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat aaatcaatat
    12001 tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt atattggctc
    12061 atgtccaata tgaccgccat gttgacattg attattgact agttattaat agtaatcaat
    12121 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
    12181 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
    12241 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
    12301 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc ctattgacgt
    12361 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac gggactttcc
    12421 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
    12481 gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
    12541 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
    12601 taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
    12661 cagagctcgt ttagtgaacc gtcagatctc tagaagctgg gtaccagctg ctagc
//
