LOCUS       sequence-235184-        8880 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of the tAKAR-zeta sensor for detecting PKA 
            activity using two-photon fluorescence lifetime imaging microscopy 
            (2pFLIM).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8880)
  AUTHORS   Ma L, Jongbloets BC, Xiong WH, Melander JB, Qin M, Lameyer TJ, 
            Harrison MF, Zemelman BV, Mao T, Zhong H
  TITLE     A Highly Sensitive A-Kinase Activity Reporter for Imaging 
            Neuromodulatory Events in Awake Mice.
  JOURNAL   Neuron. 2018 Aug 22;99(4):665-679.e5. doi: 
            10.1016/j.neuron.2018.07.020. Epub 2018 Aug 9.
  PUBMED    30100256
REFERENCE   2  (bases 1 to 8880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2018.07.020"; journalName: "Neuron"; date: 
            "2018-08-22- 22"; volume: "99"; issue: "4"; pages: "665-679.e5"
COMMENT     Sequence Label: pRK5_tAKAR-zeta
FEATURES             Location/Qualifiers
     source          1..8880
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        14..393
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        394..597
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     548..568
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     594..618
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        828..846
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     828..845
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     859..878
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      3181..3190
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3187..3189,3190..3192,3193..3705)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     CDS             join(4609..4611,4612..4614,4615..5127)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNWNSVNVYIMADKQKNGIK
                     VNFKIRHNIE"
     regulatory      5180..5189
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    5193..5327
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5230..5249)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5284..5303
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        5396..5753
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5396..5587
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5419..5439)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5604..5739
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5773..5790)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5773..5789)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5782..5804)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6002..6457
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6089..6108)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6299..6320
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6474..6493)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6593..6615
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6653..6671)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6739..6843
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6844..6912,6913..7704)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7062..7081)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7875..8463
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8364..8383
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8617..8634
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8751..8772
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8787..8792,8793..8810,8811..8817)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8825..8841
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8830..8852
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8849..8865
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8849..8865
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tacggggtca
       61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
      601 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
      661 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt
      721 gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta
      781 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
      841 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc
      901 acctcggttc tatcgattga attcgattaa ctccaatgaa gtcagagccc cctactcgcc
      961 gccgcggcca ggccccccaa catggactgt ctctgtatag tgacaaccaa gaaataccgc
     1021 taccaagatg aagacacgcc ccctctggaa cacagcccgg cccacctccc caaccaggcc
     1081 aattctcccc ctgtgattgt caacacggac accctagaag ccccaggata tgagttgcag
     1141 gtgaatggaa cagaggggga gatggagtat gaggagatca cattggaaag gggtaactca
     1201 ggtctgggct tcagcatcgc aggtggcact gacaacccgc acatcggtga cgacccgtcc
     1261 atttttatca ccaagatcat tcctggtggg gctgcagccc aggatggccg cctcagggtc
     1321 aatgacagca tcctgtttgt aaatgaagtg gatgttcggg aggtgaccca ttcagctgcg
     1381 gtggaggccc tcaaagaggc aggttccatc gttcgcctct atgtcatgcg ccggaaaccc
     1441 ccagccgaaa aggtcatgga gatcaaactc atcaaagggc ctaaaggact tggcttcagc
     1501 attgcggggg gcgttgggaa ccagcacatc cctggagata acagcatcta tgtaacaaag
     1561 atcatcgaag gaggtgctgc ccacaaggat ggcaggttgc agattggaga caagatcctg
     1621 gcggtcaaca gtgtggggct ggaggacgtc atgcacgagg atgccgtggc agccctgaag
     1681 aacacatatg acgttgtgta cctaaaggtg gccaagccca gcaatgccta cctgagtgac
     1741 agctatgctc ccccagacat cacaacctcg tattctcagc acctggacaa tgagatcagt
     1801 catagcagct acttgggcac tgactacccc acagccatga cccccacttc ccctcggcgc
     1861 tactcccctg tggccaagga cctgctgggg gaggaagaca ttccccggga accaaggcgg
     1921 atcgtgatcc atcggggctc caccggcctg ggcttcaaca ttgtgggcgg cgaggatggt
     1981 gaaggcatct tcatctcctt catccttgct gggggtccag ccgacctcag tggggagcta
     2041 cggaaggggg accagatcct gtcggtcaat ggtgttgacc tccgcaatgc cagtcacgaa
     2101 caggctgcca ttgccctgaa gaatgcgggt cagacggtca cgatcatcgc tcagtataaa
     2161 ccagaagagt atagtcgatt cgaggccaag atccatgatc ttcgggaaca gctcatgaat
     2221 agtagcctag gctcagggac tgcatccttg cgaagcaacc ccaagagggg cttctacatt
     2281 agggccctgt ttgattacga caagaccaag gactgcggtt tcttgagcca ggccctgagc
     2341 ttccgcttcg gggatgtgct tcatgtcatt gacgctggtg acgaagagtg gtggcaagca
     2401 cggcgggtcc actccgacag tgagaccgac gacattggct tcattcccag caaacggcgg
     2461 gtcgagcgac gagagtggtc aaggttaaag gccaaggact ggggctccag ctctggatca
     2521 cagggtcgag aagactcggt tctgagctat gagacggtga cccagatgga agtgcactat
     2581 gctcgtccca tcatcatcct tggacccacc aaagaccgtg ccaacgatga tcttctctcc
     2641 gagttccccg acaagtttgg atcctgtgtc cctcatacga cacgtcctaa gcgggaatat
     2701 gagatagacg gccgggatta ccactttgtc tcctcccggg agaaaatgga gaaggacatc
     2761 caggcacaca agttcattga ggctggccag tacaacagcc acctctatgg gaccagcgtc
     2821 cagtctgtgc gagaggtagc agagcagggg aagcactgca tcctcgatgt ctcggccaat
     2881 gccgtgcggc ggctgcaggc ggcccacctg caccccatcg ccatcttcat ccgtccccgc
     2941 tccctggaga atgtgctaga gatcaataag cggatcacag aggagcaagc ccggaaagcc
     3001 ttcgacagag ccacgaagct ggagcaggag ttcacagagt gcttctcagc catcgtagag
     3061 ggcgacagct ttgaagagat ctatcacaaa gtgaaacgtg tcattgaaga cctctcaggc
     3121 ccctacatct gggtcccagc ccgagagaga ctggtaccgc gggcccggga tccaccggtc
     3181 gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     3241 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     3301 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     3361 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     3421 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     3481 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     3541 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     3601 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     3661 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     3721 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     3781 aaccactacc tgagcaccca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac
     3841 atggtcctgc tggagttcgt gaccgccgcc cgcatgcata agttttctca agaacagatc
     3901 ggcgaaaaca ttgtgtgcag ggtcatttgt accacgggtc aaattcccat ccgagatttg
     3961 tcagctgata tttcacaagt gcttaaggaa aaacgatcca taaagaaagt ttggacattt
     4021 ggtagaaacc cagcctgtga ctatcattta ggaaacattt caagactgtc aaataagcat
     4081 ttccaaatac tactaggaga agacggtaac cttttattga atgacatttc cactaatggg
     4141 acctggttaa atgggcaaaa agtcgagaag aacagcaatc agttactgtc tcaaggtgat
     4201 gaaataaccg ttggtgtagg cgtggaatca gatattttat ctctggtcat tttcataaac
     4261 gacaaattta agcagtgcct cgagcagaac aaagttgatc gctctgcagg taagccaggc
     4321 agcggcgagg gcagcaccaa gggcctgcgt cgcgccaccc tggtggacgg cggcaccggc
     4381 ggcagcgagc tcatgggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     4441 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgagc
     4501 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agagagtgac cgccgccggg
     4561 atcactctcg gcatggacga gctatacaag ggtggaactg gaggttcaat ggtgagcaag
     4621 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     4681 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     4741 ctgaagctca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     4801 ttcggctacg gcctgatgtg cttcgcccgc taccccgacc acatgaagca gcacgacttc
     4861 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     4921 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     4981 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     5041 aactggaaca gcgtcaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     5101 aacttcaaga tccgccacaa catcgagtaa gcggccgcaa ttccccgggg atcctctaga
     5161 gtcgacctgc agaagcttgg ccgccatggc ccaacttgtt tattgcagct tataatggtt
     5221 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     5281 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcggg aattaattcg
     5341 gcgcagcacc atggcctgaa ataacctctg aaagaggaac ttggttaggt accttctgag
     5401 gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     5461 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     5521 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     5581 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     5641 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     5701 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctgt
     5761 taacagcttg gcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     5821 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     5881 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg
     5941 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag
     6001 tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     6061 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     6121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     6181 agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg
     6241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     6301 ggactcttgt tccaaactgg aacaacactc aaccctatct cgggctattc ttttgattta
     6361 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     6421 aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc
     6481 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
     6541 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     6601 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
     6661 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     6721 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     6781 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     6841 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     6901 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     6961 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     7021 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     7081 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     7141 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     7201 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     7261 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     7321 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     7381 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     7441 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     7501 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     7561 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     7621 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     7681 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     7741 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     7801 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     7861 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     7921 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     7981 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     8041 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     8101 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     8161 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     8221 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     8281 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     8341 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     8401 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     8461 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     8521 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     8581 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     8641 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     8701 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     8761 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     8821 ggaattgtga gcggataaca atttcacaca ggaaacagct atgacatgat tacgaattaa
//
