LOCUS       sequence-23519-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Zhou BY, Ye Z, Chen G, Gao ZP, Zhang YA, Cheng L
  TITLE     Inducible and reversible transgene expression in human stem cells 
            after efficient and stable gene transfer.
  JOURNAL   Stem Cells. 2007 Mar . 25(3):779-89.
  PUBMED    17158240
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cells.
            2007 Mar . 25(3):779-89."
COMMENT     Sequence Label: Duet011
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(221..240)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
ORIGIN
        1 tacgctctgc ccttgctcac catggtggcg accggtaccc ggggatcctc tagagtcgac
       61 ctgcagccca agcttcgtct aacaaaaaag ccaaaaacgg ccagaattta gcggacaatt
      121 tactagtcta acactgaaaa ttacatattg acccaaatga ttacatttca aaaggtgcct
      181 aaaaaacttc acaaaacaca ctcgccaacc ccgagcgcat agttcaaaac cggagcttca
      241 gctacttaag aagataggta cataaaaccg accaaagaaa ctgacgcctc acttatccct
      301 cccctcacca gaggtccggc gcctgtcgat tcaggagagc ctaccctagg cccgaaccct
      361 gcgtcctgcg acggagaaaa gcctaccgca cacctaccgg caggtggccc caccctgcat
      421 tataagccaa cagaacgggt gacgtcacga cacgacgagg gcgcgcgctc ccaaaggtac
      481 gggtgcactg cccaacggca ccgccataac tgccgccccc gcaacagacg acaaaccgag
      541 ttctccagtc agtgacaaac ttcacgtcag ggtccccaga tggtgcccca gcccatctca
      601 cccgaataag agctttcccg cattagcgaa ggcctcaaga ccttgggttc ttgccgccca
      661 ccatgccccc caccttgttt caacgacctc acagcccgcc tcacaagcgt cttccattca
      721 agactcggga acagccgcca ttttgctgcg ctccccccaa cccccagttn agggcaacct
      781 ngctngcgga cccagactac agcccntggg ggtctctcca
//
