LOCUS       sequence-235191-       12937 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the 191084 B7-19 HIV-1 
            isolate; QES mutant with enhanced binding to PG16.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12937)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 12937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-191084-QES.i06.c07
FEATURES             Location/Qualifiers
     source          1..12937
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    2564..2698
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2588..2607)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2642..2661
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2840..2862)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      3448..5237
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(5543..7468)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(7903..7921)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7989..8093
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8094..8162,8163..8954)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8312..8331)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9125..9713
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9614..9633
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        10330..10475
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             10515..11540
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             10515..11540
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(11561..11580)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11605..11653
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        12306..12685
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12686..12889
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     12840..12860
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     12886..12910
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gccaccatgc cgatggggtc tctccagccg ctggccaccc tctacctgtt gggtatgttg
       61 gtggcgtccg tgctggcgac agagaatctg tgggttacag tctactacgg agttccagtc
      121 tggagggacg cagagacaac acttttttgt gccagcgacg ctaaagcgta tgacactgaa
      181 atgcataatg tctgggcaac tcatgcctgt gttcccacag acccgaatcc acaagaaata
      241 gacttggaga atgtaacgga gaaatttaac atgtggaaaa ataacatggt tgaacaaatg
      301 catacggata taatcagcct ttgggaccaa tccctgaagc cgtgcgttaa gttgacagac
      361 ttgtgtgtta ctctgaactg cacagccatt accaatgata cgagggggaa tgagactggt
      421 atcaatcgga ccgtcgagac gaccgaaatg accaactgtt cattcaatat gaccactgaa
      481 cttagggacc gaaaaaagaa ggtcaatgcg cttttttata agcttgacat cgttcagata
      541 ggagaaaatt catctagcca gtaccgcctc attaattgta ataccagtgt tattacacaa
      601 gcctgcccaa aaatgacctt cgagccaatt ccaattcatt attgcgcgcc tgctggattt
      661 gccatcctta agtgcaagga caaagagttc aacgggacag gaacttgtag gaacgtgtcc
      721 agtgtgcaat gcactcacgg tataaaaccc gtcatgagca cgcaactttt gctcaacggg
      781 tccctggcgg aaggacaggt gaagatccgg tcagagaaca tctcagacaa tgcaaaaacc
      841 atcatcgtcc agttgaatga gtctgttcca atcaattgta ctagaccaaa caacaataca
      901 cgaaggggga tccacctggg tccgggccaa acattctttg ctaccgacat aattggtgac
      961 attcgccaag ctcactgtaa tgttagcgaa tcaaaatgga acaaggcgct ccaggaagtt
     1021 gtgaaacagc tgaggcagca ctggaacaaa acgataatct tcaaatcctc atccggtggg
     1081 gatctcgaaa tcacgactca ctccttcaac tgtggaggtg agtggttcta ctgtaacacc
     1141 tcaggcctgt tcaatagcac atggaacata gccggcaaca gaacgaatga tacgaagtca
     1201 aatgaaacaa tcaccctccc gtgtcgaata aaacagatcg taaatgtttg gcaaagggta
     1261 ggacaagcga tctacgcgcc cccaattgca ggagtcattc ggtgcaattc aaacatcaca
     1321 ggcctcctct tggtaagaga tgggggagct actaataata cagacgaaac gttccgccct
     1381 ggaggcggga acatgcgcga taattggaga agtgagttgt ataaatataa ggtggtcaag
     1441 atagagcctc tgggcgttgc accgaccagg gcgcgcaggc gcgtggtgga aagggagaag
     1501 agggctgtag gactcggagc cgtatttatt ggatttcttg gggcagcggg ttctaccatg
     1561 ggtgcagctt ccgtgacctt gaccgtacaa gccaggcaat atctttctgg aatcgttcaa
     1621 caacaaagca atcttctcca ggcaatcgag gcccagcagc atctcttgaa acttacagtc
     1681 tggggcataa agcagctcca ggcgcgcgtg ctcgcagttg agagatattt gaaagaccaa
     1741 cagctgcttg gtatttgggg ctgttctgga aaattgatat gcccgactac cgtaccatgg
     1801 aacagtagct ggtcaaacaa atcacaaaac gaaatctggg ataatatgac ctggcttcaa
     1861 tgggacaagg agatttcaaa ttacacacag cttatatatt cattgattga ggaaagccaa
     1921 aatcagcagg agaagaacga gcaggagctg ttggcgctgg acaagtgggc atctttgtgg
     1981 aattggttcg acatttccaa gtggctttgg tatatcagaa tatttataat gatcgtgggc
     2041 gggttgatag gacttagaat tgtattcaca gttctcagca tcattaatag ggttcgccag
     2101 ggttactccc cgctctcttt tcagacacat ccgccatctc caacaggtcc tgataggcct
     2161 gggcggattg aagaagaggg tggtgagcaa ggacgggata gaagcaccag gctggtatct
     2221 ggattcttgg cattggcctg ggatgatctc agatccctct tcctctttag ttatcatcac
     2281 ctccgagatc tcatcctcat cgtcgcaagg gcggtcgagc ttctgggcca taatagtctt
     2341 aagggactta aacttggctg gaaccttctc ctgtactggt ctagagagtt gaagatttcc
     2401 gcgttgaatt tgttggatac cattgccatc gcagtggcgg gttggaccga ccgagttatc
     2461 gagatagggc agagaatttg ccgggcaata cggaatattc caagacgaat tagacagggc
     2521 gcggaaagag catttctgta gtaactcgag gccggcaagg ccggatccag acatgataag
     2581 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     2641 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     2701 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgggga ggttttttaa
     2761 agcaagtaaa acctctacaa atgtggtatg gctgattatg atccggctgc ctcgcgcgtt
     2821 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     2881 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     2941 gtcggggcgc agccatgacc ggtcgactct agagccccgc cgccggacga actaaacctg
     3001 actacggcat ctctgcccct tcttcgctgg tacgaggagc gcttttgttt tgtattcggg
     3061 gcagtgcatg taatcccttc agttggttgg tacaacttgc caactgggcc ctgttccaca
     3121 tgtgacacgg ggggggacca aacacaaagg ggttctctga ctgtagttga catccttata
     3181 aatggatgtg cacatttgcc aacactgagt ggctttcatc ctggagcaga ctttgcatgc
     3241 tgtggactgc aacacaacat tgcctttatg tgtaactctt ggctgaagct cttacaccaa
     3301 tgctggggga catgtacctc ccaggggccc aggaagacta cgggaggcta caccaacgtc
     3361 aatcagaggg gcctgtgtag ctaccgataa gcggaccctc aagagggcat tagcaatagt
     3421 gtttataagg cccccttgtt aaccctaaac gggtagcata tgcttcccgg gtagtagtat
     3481 atactatcca gactaaccct aattcaatag catatgttac ccaacgggaa gcatatgcta
     3541 tcgaattagg gttagtaaaa gggtcctaag gaacagcgat atctcccacc ccatgagctg
     3601 tcacggtttt atttacatgg ggtcaggatt ccacgagggt agtgaaccat tttagtcaca
     3661 agggcagtgg ctgaagatca aggagcgggc agtgaactct cctgaatctt cgcctgcttc
     3721 ttcattctcc ttcgtttagc taatagaata actgctgagt tgtgaacagt aaggtgtatg
     3781 tgaggtgctc gaaaacaagg tttcaggtga cgcccccaga ataaaatttg gacggggggt
     3841 tcagtggtgg cattgtgcta tgacaccaat ataaccctca caaacccctt gggcaataaa
     3901 tactagtgta ggaatgaaac attctgaata tctttaacaa tagaaatcca tggggtgggg
     3961 acaagccgta aagactggat gtccatctca cacgaattta tggctatggg caacacataa
     4021 tcctagtgca atatgatact ggggttatta agatgtgtcc caggcaggga ccaagacagg
     4081 tgaaccatgt tgttacactc tatttgtaac aaggggaaag agagtggacg ccgacagcag
     4141 cggactccac tggttgtctc taacaccccc gaaaattaaa cggggctcca cgccaatggg
     4201 gcccataaac aaagacaagt ggccactctt ttttttgaaa ttgtggagtg ggggcacgcg
     4261 tcagccccca cacgccgccc tgcggttttg gactgtaaaa taagggtgta ataacttggc
     4321 tgattgtaac cccgctaacc actgcggtca aaccacttgc ccacaaaacc actaatggca
     4381 ccccggggaa tacctgcata agtaggtggg cgggccaaga taggggcgcg attgctgcga
     4441 tctggaggac aaattacaca cacttgcgcc tgagcgccaa gcacagggtt gttggtcctc
     4501 atattcacga ggtcgctgag agcacggtgg gctaatgttg ccatgggtag catatactac
     4561 ccaaatatct ggatagcata tgctatccta atctatatct gggtagcata ggctatccta
     4621 atctatatct gggtagcata tgctatccta atctatatct gggtagtata tgctatccta
     4681 atttatatct gggtagcata ggctatccta atctatatct gggtagcata tgctatccta
     4741 atctatatct gggtagtata tgctatccta atctgtatcc gggtagcata tgctatccta
     4801 atagagatta gggtagtata tgctatccta atttatatct gggtagcata tactacccaa
     4861 atatctggat agcatatgct atcctaatct atatctgggt agcatatgct atcctaatct
     4921 atatctgggt agcataggct atcctaatct atatctgggt agcatatgct atcctaatct
     4981 atatctgggt agtatatgct atcctaattt atatctgggt agcataggct atcctaatct
     5041 atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct atcctaatct
     5101 gtatccgggt agcatatgct atcctcatgc atatacagtc agcatatgat acccagtagt
     5161 agagtgggag tgctatcctt tgcatatgcc gccacctccc aagggggcgt gaattttcgc
     5221 tgcttgtcct tttcctgcat gctggttgct cccattctta ggtgaattta aggaggccag
     5281 gctaaagccg tcgcatgtct gattgctcac caggtaaatg tcgctaatgt tttccaacgc
     5341 gagaaggtgt tgagcgcgga gctgagtgac gtgacaacat gggtatgccc aattgcccca
     5401 tgttgggagg acgaaaatgg tgacaagaca gatggccaga aatacaccaa cagcacgcat
     5461 gatgtctact ggggatttat tctttagtgc gggggaatac acggctttta atacgattga
     5521 gggcgtctcc taacaagtta catcactcct gcccttcctc accctcatct ccatcacctc
     5581 cttcatctcc gtcatctccg tcatcaccct ccgcggcagc cccttccacc ataggtggaa
     5641 accagggagg caaatctact ccatcgtcaa agctgcacac agtcaccctg atattgcagg
     5701 taggagcggg ctttgtcata acaaggtcct taatcgcatc cttcaaaacc tcagcaaata
     5761 tatgagtttg taaaaagacc atgaaataac agacaatgga ctcccttagc gggccaggtt
     5821 gtgggccggg tccaggggcc attccaaagg ggagacgact caatggtgta agacgacatt
     5881 gtggaatagc aagggcagtt cctcgcctta ggttgtaaag ggaggtctta ctacctccat
     5941 atacgaacac accggcgacc caagttcctt cgtcggtagt cctttctacg tgactcctag
     6001 ccaggagagc tcttaaacct tctgcaatgt tctcaaattt cgggttggaa cctccttgac
     6061 cacgatgctt tccaaaccac cctccttttt tgcgcctgcc tccatcaccc tgaccccggg
     6121 gtccagtgct tgggccttct cctgggtcat ctgcggggcc ctgctctatc gctcccgggg
     6181 gcacgtcagg ctcaccatct gggccacctt cttggtggta ttcaaaataa tcggcttccc
     6241 ctacagggtg gaaaaatggc cttctacctg gagggggcct gcgcggtgga gacccggatg
     6301 atgatgactg actactggga ctcctgggcc tcttttctcc acgtccacga cctctccccc
     6361 tggctctttc acgacttccc cccctggctc tttcacgtcc tctaccccgg cggcctccac
     6421 tacctcctcg accccggcct ccactacctc ctcgaccccg gcctccactg cctcctcgac
     6481 cccggcctcc acctcctgct cctgcccctc ctgctcctgc ccctcctcct gctcctgccc
     6541 ctcctgcccc tcctgctcct gcccctcctg cccctcctgc tcctgcccct cctgcccctc
     6601 ctgctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctgcccct cctcctgctc
     6661 ctgcccctcc tgcccctcct gctcctgccc ctcctgcccc tcctgctcct gcccctcctg
     6721 cccctcctgc tcctgcccct cctgctcctg cccctcctgc tcctgcccct cctgctcctg
     6781 cccctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctgctcct gcccctcctg
     6841 cccctcctgc ccctcctgct cctgcccctc ctcctgctcc tgcccctcct gcccctcctg
     6901 cccctcctcc tgctcctgcc cctcctgccc ctcctcctgc tcctgcccct cctcctgctc
     6961 ctgcccctcc tgcccctcct gcccctcctc ctgctcctgc ccctcctgcc cctcctcctg
     7021 ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc tgcccctcct cctgctcctg
     7081 cccctcctcc tgctcctgcc cctcctgccc ctcctgcccc tcctgcccct cctcctgctc
     7141 ctgcccctcc tcctgctcct gcccctcctg ctcctgcccc tcccgctcct gctcctgctc
     7201 ctgttccacc gtgggtccct ttgcagccaa tgcaacttgg acgtttttgg ggtctccgga
     7261 caccatctct atgtcttggc cctgatcctg agccgcccgg ggctcctggt cttccgcctc
     7321 ctcgtcctcg tcctcttccc cgtcctcgtc catggttatc accccctctt ctttgaggtc
     7381 cactgccgcc ggagccttct ggtccagatg tgtctccctt ctctcctagg ccatttccag
     7441 gtcctgtacc tggcccctcg tcagacatga ttcacactaa aagagatcaa tagacatctt
     7501 tattagacga cgctcagtga atacagggag tgcagactcc tgccccctcc aacagccccc
     7561 ccaccctcat ccccttcatg gtcgctgtca gacagatcca ggtctgaaaa ttccccatcc
     7621 tccgaaccat cctcgtcctc atcaccaatt actcgcagcc cggaaaactc ccgctgaaca
     7681 tcctcaagat ttgcgtcctg agcctcaagc caggcctcaa attcctcgtc cccctttttg
     7741 ctggacggta gggatgggga ttctcgggac ccctcctctt cctcttcaag gtcaccagac
     7801 agagatgcta ctggggcaac ggaagaaaag ctgggtgcgg cctgtgagga tcagcttatc
     7861 gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat
     7921 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     7981 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     8041 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     8101 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     8161 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     8221 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     8281 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg
     8341 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     8401 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     8461 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     8521 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     8581 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag
     8641 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     8701 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     8761 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     8821 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     8881 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     8941 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     9001 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     9061 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     9121 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     9181 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     9241 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     9301 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     9361 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     9421 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     9481 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     9541 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     9601 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     9661 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     9721 gcaacgcggc ctttttacgg ttcctggcct tttgctgcgc cgcgtgcggc tgctggagat
     9781 ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa
     9841 gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc
     9901 attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg
     9961 tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa
    10021 atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat
    10081 ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg
    10141 ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc
    10201 gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccctg cttcatcccc
    10261 gtggcccgtt gctcgcgttt gctggcggtg tccccggaag aaatatattt gcatgtcttt
    10321 agttctatga tgacacaaac cccgcccagc gtcttgtcat tggcgaattc gaacacgcag
    10381 atgcagtcgg ggcggcgcgg tcccaggtcc acttcgcata ttaaggtgac gcgtgtggcc
    10441 tcgaacaccg agcgaccctg cagcgacccg cttaacagcg tcaacagcgt gccgcagatc
    10501 ccgggcaatg agatatgaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga
    10561 tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg
    10621 ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg
    10681 gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg
    10741 aagtgcttga cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac
    10801 agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg
    10861 cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat
    10921 tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg
    10981 atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc
    11041 aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc
    11101 acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg
    11161 actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga
    11221 ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc
    11281 ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc
    11341 agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa
    11401 tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg
    11461 tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc
    11521 gtccgagggc aaaggaatag gggagatggg ggaggctaac tgaaacacgg aaggagacaa
    11581 taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacgggtgtt
    11641 gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca
    11701 ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca
    11761 agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc
    11821 actggccccg tgggttaggg acggggtccc ccatggggaa tggtttatgg ttcgtggggg
    11881 ttattatttt gggcgttgcg tggggtctgg tccacgactg gactgagcag acagacccat
    11941 ggtttttgga tggcctgggc atggaccgca tgtactggcg cgacacgaac accgggcgtc
    12001 tgtggctgcc aaacaccccc gacccccaaa aaccaccgcg cggatttctg gcgtgccaag
    12061 ctagtcgacc aattctcatg tttgacagct tatcatcgca gatccgggca acgttgttgc
    12121 attgctgcag gcgcagaact ggtaggtatg gaagatctat acattgaatc aatattggca
    12181 attagccata ttagtcattg gttatatagc ataaatcaat attggctatt ggccattgca
    12241 tacgttgtat ctatatcata atatgtacat ttatattggc tcatgtccaa tatgaccgcc
    12301 atgttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
    12361 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
    12421 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
    12481 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
    12541 acatcaagtg tatcatatgc caagtccgcc ccctattgac gtcaatgacg gtaaatggcc
    12601 cgcctggcat tatgcccagt acatgacctt acgggacttt cctacttggc agtacatcta
    12661 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacacca atgggcgtgg
    12721 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
    12781 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aataaccccg ccccgttgac
    12841 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa
    12901 ccgtcagatc tctagaagct gggtaccagc tgctagc
//
