LOCUS       sequence-235192-       12458 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the B41 HIV-1 isolate; 
            C-terminal truncation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12458)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 12458)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-B41gp160-754*
FEATURES             Location/Qualifiers
     source          1..12458
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1586..1597
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     polyA_signal    2303..2437
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2327..2346)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2381..2400
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2580..2602)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      3162..4951
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(5257..7182)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(7617..7635)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7703..7807
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7808..7876,7877..8668)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8026..8045)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8839..9427
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9328..9347
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        9858..10003
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             10046..11083
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(11081..11100)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11125..11173
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        11827..12206
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12207..12410
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     12361..12381
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     12407..12431
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gccaccatgc caatgggtag tcttcagcct ctcgccacct tgtatctgct cggtatgctg
       61 gttgccagcg ttctggctaa cttgtgggtg accgtatact acggggtccc ggtatggaag
      121 gaggcaaaca ctacactctt ctgtgcttcc gacgcaaagg ggtatgatac ggagaagcat
      181 aatgtctggg cgacacatgc atgtgtgcct acagacccaa acccgcagga agtcctcctt
      241 ggtaataacg tgaccgagaa ctttaatatg tggaaaaata acatggtaga gcagatgcat
      301 gaagacatta taagcttgtg ggatcaatct cttaagccgt gtgtgaagct gacaccgctt
      361 tgcgtaacac tcaattgtac ggatttgacg aaaaatagca ctaccaataa ctccaccgct
      421 actaacaata ccacgaacaa tagtaccgaa aaaatggaga tggacaaagg cgagatgaag
      481 aattgctcct ttaatatcac aacaaatatt cgagataaga tgcagaaaga atacgctttg
      541 ctgtacaagc ttgacatcgt gccgatcgac aatggcaaga ataacagcaa caccaattat
      601 cgcctgattt catgtaatac ctcagtaatt acgcaatcat gtcccaaagt gtcatttcag
      661 cccattccta ttcattattg tgcacccgcc ggttttgcaa tacttaagtg taataataag
      721 acctttaatg gaagcgggcc ttgtactaat gtgtcttccg tgcaatgcac tcacggtatc
      781 cgacctgttg tttcaaccca gcttttgctg aacggaagtc tcgctgaaga agaaatcgtg
      841 attaggtccg ctaacttctc tgacaacacg aagactataa ttgtccaact taaggaagcg
      901 gtcgaaataa actgtacgcg cccgaataat aacacgcgaa aatcaattaa catcgggcct
      961 gggcgagctt tttatgctac tggcaacata ataggggaca tccgccaagc tcattgtaac
     1021 atatcacgga caaagtggaa taatacgttg acagaaattg ctaagaagct gaacaagcag
     1081 tacgagaata gaacaatagc atttaatcaa tcctccggag gcgatcttga gatcgtcatg
     1141 cattcattca actgcggtgg agaatttttc tattgcaaca cttcccagct gttcagagat
     1201 acgtggaaca acacttggaa cggcatatgg aacaacgata cggaggtcaa cgagactatc
     1261 acacttccgt gtagaatcaa acaaataatc aatatgtggc aagaagtcgg gaaggcgatg
     1321 tatgccccgc caatccgcgg ccaaatcaat tgctcatcaa atattacggg actcctgctc
     1381 acccgcgacg ggggagaaag caacaagacc atagaggtct tccggcccgg tggcggagac
     1441 atgagggaca actggaggtc cgagctgtat aagtacaaag ttgttaagat agagcccctc
     1501 ggggttgctc ccaccaaagc taaaagaaga gtggtgcaac gagagaaaag agccgtcggc
     1561 ttgggtgccc tcttcgtcgg atttttggga gctgcaggta gcacaatggg tgctgcctct
     1621 ataacgctga ccgtacaagc tcgacagctt cttagtggta tcgttcaaca acaaaacaat
     1681 ctcttgcgag caatagaagc ccaacagcat ttgcttcaac tgacagtatg gggcgttaag
     1741 cagttgcaag cgcgggtctt ggcggtcgaa cgatacctga aagaccagca actcctgggc
     1801 ttgtggggat gcagcggcaa acttatatgt accacagctg taccttggaa caccagctgg
     1861 agtaacaaga gttacgatta tatctgggac aacatgacgt ggatgcaatg ggagcgcgag
     1921 attgataact atacaggagt tatctacaag ctgattgagg aatcccagaa ccaacaggag
     1981 aaaaatgaac aagaactttt ggccctggat aaatgggcaa atctgtggaa ctggtttgac
     2041 atcacaagat ggctttggta cattaagatt tttattatga tcgtaggggg tttggtaggg
     2101 cttcggatcg tttttgctgt gctctccatc gtgaaccgag tcaggcaggg gtactcacct
     2161 ttgagcctgc aaacgcactt tcctgcgcca agaggaccgg atcgaccaga gggaacggag
     2221 gaggaaggtg gtgaccggga tcgggatcgc agcactcccc tcgtggatgg gttcctttga
     2281 taactcgagg ccggcaaggc cggatccaga catgataaga tacattgatg agtttggaca
     2341 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     2401 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     2461 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa
     2521 atgtggtatg gctgattatg atccggctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     2581 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     2641 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgagg
     2701 tcgactctag aggatcgatc cccgccccgg acgaactaaa cctgactacg acatctctgc
     2761 cccttcttcg cggggcagtg catgtaatcc cttcagttgg ttggtacaac ttgccaactg
     2821 ggccctgttc cacatgtgac acgggggggg accaaacaca aaggggttct ctgactgtag
     2881 ttgacatcct tataaatgga tgtgcacatt tgccaacact gagtggcttt catcctggag
     2941 cagactttgc agtctgtgga ctgcaacaca acattgcctt tatgtgtaac tcttggctga
     3001 agctcttaca ccaatgctgg gggacatgta cctcccaggg gcccaggaag actacgggag
     3061 gctacaccaa cgtcaatcag aggggcctgt gtagctaccg ataagcggac cctcaagagg
     3121 gcattagcaa tagtgtttat aaggccccct tgttaaccct aaacgggtag catatgcttc
     3181 ccgggtagta gtatatacta tccagactaa ccctaattca atagcatatg ttacccaacg
     3241 ggaagcatat gctatcgaat tagggttagt aaaagggtcc taaggaacag cgatatctcc
     3301 caccccatga gctgtcacgg ttttatttac atggggtcag gattccacga gggtagtgaa
     3361 ccattttagt cacaagggca gtggctgaag atcaaggagc gggcagtgaa ctctcctgaa
     3421 tcttcgcctg cttcttcatt ctccttcgtt tagctaatag aataactgct gagttgtgaa
     3481 cagtaaggtg tatgtgaggt gctcgaaaac aaggtttcag gtgacgcccc cagaataaaa
     3541 tttggacggg gggttcagtg gtggcattgt gctatgacac caatataacc ctcacaaacc
     3601 ccttgggcaa taaatactag tgtaggaatg aaacattctg aatatcttta acaatagaaa
     3661 tccatggggt ggggacaagc cgtaaagact ggatgtccat ctcacacgaa tttatggcta
     3721 tgggcaacac ataatcctag tgcaatatga tactggggtt attaagatgt gtcccaggca
     3781 gggaccaaga caggtgaacc atgttgttac actctatttg taacaagggg aaagagagtg
     3841 gacgccgaca gcagcggact ccactggttg tctctaacac ccccgaaaat taaacggggc
     3901 tccacgccaa tggggcccat aaacaaagac aagtggccac tctttttttt gaaattgtgg
     3961 agtgggggca cgcgtcagcc cccacacgcc gccctgcggt tttggactgt aaaataaggg
     4021 tgtaataact tggctgattg taaccccgct aaccactgcg gtcaaaccac ttgcccacaa
     4081 aaccactaat ggcaccccgg ggaatacctg cataagtagg tgggcgggcc aagatagggg
     4141 cgcgattgct gcgatctgga ggacaaatta cacacacttg cgcctgagcg ccaagcacag
     4201 ggttgttggt cctcatattc acgaggtcgc tgagagcacg gtgggctaat gttgccatgg
     4261 gtagcatata ctacccaaat atctggatag catatgctat cctaatctat atctgggtag
     4321 cataggctat cctaatctat atctgggtag catatgctat cctaatctat atctgggtag
     4381 tatatgctat cctaatttat atctgggtag cataggctat cctaatctat atctgggtag
     4441 catatgctat cctaatctat atctgggtag tatatgctat cctaatctgt atccgggtag
     4501 catatgctat cctaatagag attagggtag tatatgctat cctaatttat atctgggtag
     4561 catatactac ccaaatatct ggatagcata tgctatccta atctatatct gggtagcata
     4621 tgctatccta atctatatct gggtagcata ggctatccta atctatatct gggtagcata
     4681 tgctatccta atctatatct gggtagtata tgctatccta atttatatct gggtagcata
     4741 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     4801 tgctatccta atctgtatcc gggtagcata tgctatcctc atgcatatac agtcagcata
     4861 tgatacccag tagtagagtg ggagtgctat cctttgcata tgccgccacc tcccaagggg
     4921 gcgtgaattt tcgctgcttg tccttttcct gcatgctggt tgctcccatt cttaggtgaa
     4981 tttaaggagg ccaggctaaa gccgtcgcat gtctgattgc tcaccaggta aatgtcgcta
     5041 atgttttcca acgcgagaag gtgttgagcg cggagctgag tgacgtgaca acatgggtat
     5101 gcccaattgc cccatgttgg gaggacgaaa atggtgacaa gacagatggc cagaaataca
     5161 ccaacagcac gcatgatgtc tactggggat ttattcttta gtgcggggga atacacggct
     5221 tttaatacga ttgagggcgt ctcctaacaa gttacatcac tcctgccctt cctcaccctc
     5281 atctccatca cctccttcat ctccgtcatc tccgtcatca ccctccgcgg cagccccttc
     5341 caccataggt ggaaaccagg gaggcaaatc tactccatcg tcaaagctgc acacagtcac
     5401 cctgatattg caggtaggag cgggctttgt cataacaagg tccttaatcg catccttcaa
     5461 aacctcagca aatatatgag tttgtaaaaa gaccatgaaa taacagacaa tggactccct
     5521 tagcgggcca ggttgtgggc cgggtccagg ggccattcca aaggggagac gactcaatgg
     5581 tgtaagacga cattgtggaa tagcaagggc agttcctcgc cttaggttgt aaagggaggt
     5641 cttactacct ccatatacga acacaccggc gacccaagtt ccttcgtcgg tagtcctttc
     5701 tacgtgactc ctagccagga gagctcttaa accttctgca atgttctcaa atttcgggtt
     5761 ggaacctcct tgaccacgat gctttccaaa ccaccctcct tttttgcgcc tgcctccatc
     5821 accctgaccc cggggtccag tgcttgggcc ttctcctggg tcatctgcgg ggccctgctc
     5881 tatcgctccc gggggcacgt caggctcacc atctgggcca ccttcttggt ggtattcaaa
     5941 ataatcggct tcccctacag ggtggaaaaa tggccttcta cctggagggg gcctgcgcgg
     6001 tggagacccg gatgatgatg actgactact gggactcctg ggcctctttt ctccacgtcc
     6061 acgacctctc cccctggctc tttcacgact tccccccctg gctctttcac gtcctctacc
     6121 ccggcggcct ccactacctc ctcgaccccg gcctccacta cctcctcgac cccggcctcc
     6181 actgcctcct cgaccccggc ctccacctcc tgctcctgcc cctcctgctc ctgcccctcc
     6241 tcctgctcct gcccctcctg cccctcctgc tcctgcccct cctgcccctc ctgctcctgc
     6301 ccctcctgcc cctcctgctc ctgcccctcc tgcccctcct cctgctcctg cccctcctgc
     6361 ccctcctcct gctcctgccc ctcctgcccc tcctgctcct gcccctcctg cccctcctgc
     6421 tcctgcccct cctgcccctc ctgctcctgc ccctcctgct cctgcccctc ctgctcctgc
     6481 ccctcctgct cctgcccctc ctgcccctcc tgcccctcct cctgctcctg cccctcctgc
     6541 tcctgcccct cctgcccctc ctgcccctcc tgctcctgcc cctcctcctg ctcctgcccc
     6601 tcctgcccct cctgcccctc ctcctgctcc tgcccctcct gcccctcctc ctgctcctgc
     6661 ccctcctcct gctcctgccc ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc
     6721 tgcccctcct cctgctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctgcccc
     6781 tcctcctgct cctgcccctc ctcctgctcc tgcccctcct gcccctcctg cccctcctgc
     6841 ccctcctcct gctcctgccc ctcctcctgc tcctgcccct cctgctcctg cccctcccgc
     6901 tcctgctcct gctcctgttc caccgtgggt ccctttgcag ccaatgcaac ttggacgttt
     6961 ttggggtctc cggacaccat ctctatgtct tggccctgat cctgagccgc ccggggctcc
     7021 tggtcttccg cctcctcgtc ctcgtcctct tccccgtcct cgtccatggt tatcaccccc
     7081 tcttctttga ggtccactgc cgccggagcc ttctggtcca gatgtgtctc ccttctctcc
     7141 taggccattt ccaggtcctg tacctggccc ctcgtcagac atgattcaca ctaaaagaga
     7201 tcaatagaca tctttattag acgacgctca gtgaatacag ggagtgcaga ctcctgcccc
     7261 ctccaacagc ccccccaccc tcatcccctt catggtcgct gtcagacaga tccaggtctg
     7321 aaaattcccc atcctccgaa ccatcctcgt cctcatcacc aattactcgc agcccggaaa
     7381 actcccgctg aacatcctca agatttgcgt cctgagcctc aagccaggcc tcaaattcct
     7441 cgtccccctt tttgctggac ggtagggatg gggattctcg ggacccctcc tcttcctctt
     7501 caaggtcacc agacagagat gctactgggg caacggaaga aaagctgggt gcggcctgtg
     7561 aggatcagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct
     7621 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     7681 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     7741 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     7801 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     7861 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     7921 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     7981 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     8041 attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     8101 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     8161 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     8221 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     8281 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     8341 cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     8401 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     8461 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     8521 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     8581 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     8641 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     8701 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     8761 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     8821 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     8881 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     8941 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     9001 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     9061 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     9121 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     9181 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     9241 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     9301 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     9361 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     9421 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttgaag
     9481 ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc
     9541 gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa
     9601 cgccagcaag acgtagccca gcgcgtcggc cccgagatgc gccgcgtgcg gctgctggag
     9661 atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc
     9721 aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccctgct
     9781 tcatccccgt ggcccgttgc tcgcgtttgc tggcggtgtc cccggaagaa atatatttgc
     9841 atgtctttag ttctatgatg acacaaaccc cgcccagcgt cttgtcattg gcgaattcga
     9901 acacgcagat gcagtcgggg cggcgcggtc cgaggtccac ttcgcatatt aaggtgacgc
     9961 gtgtggcctc gaacaccgag cgaccctgca gcgacccgct taacagcgtc aacagcgtgc
    10021 cgcagatccc ggggggcaat gagatatgaa aaagcctgaa ctcaccgcga cgtctgtcga
    10081 gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga
    10141 agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag
    10201 ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct
    10261 cccgattccg gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc
    10321 ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct
    10381 gcagccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg
    10441 gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg
    10501 cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc
    10561 gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg
    10621 gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac
    10681 agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat
    10741 cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag
    10801 gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga
    10861 ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg
    10921 atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag
    10981 aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg
    11041 ccccagcact cgtccggatc gggagatggg ggaggctaac tgaaacacgg aaggagacaa
    11101 taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacgggtgtt
    11161 gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca
    11221 ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca
    11281 agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc
    11341 actggccccg tgggttaggg acggggtccc ccatggggaa tggtttatgg ttcgtggggg
    11401 ttattatttt gggcgttgcg tggggtcagg tccacgactg gactgagcag acagacccat
    11461 ggtttttgga tggcctgggc atggaccgca tgtactggcg cgacacgaac accgggcgtc
    11521 tgtggctgcc aaacaccccc gacccccaaa aaccaccgcg cggatttctg gcgtgccaag
    11581 ctagtcgacc aattctcatg tttgacagct tatcatcgca gatccgggca acgttgttgc
    11641 cattgctgca ggcgcagaac tggtaggtat ggaagatcta tacattgaat caatattggc
    11701 aattagccat attagtcatt ggttatatag cataaatcaa tattggctat tggccattgc
    11761 atacgttgta tctatatcat aatatgtaca tttatattgg ctcatgtcca atatgaccgc
    11821 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
    11881 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
    11941 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
    12001 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
    12061 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
    12121 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
    12181 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
    12241 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
    12301 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
    12361 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
    12421 accgtcagat ctctagaagc tgggtaccag ctgctagc
//
