LOCUS       sequence-235194-       12454 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the B41 HIV-1 isolate; 
            C-terminal truncation; QES mutant with enhanced binding to PG16.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12454)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 12454)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-B41gp160-V200E-754*
FEATURES             Location/Qualifiers
     source          1..12454
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1586..1597
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     polyA_signal    2303..2437
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2327..2346)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2381..2400
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2580..2602)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      3162..4951
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(5253..7178)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(7613..7631)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7699..7803
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7804..7872,7873..8664)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8022..8041)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8835..9423
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9324..9343
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        9854..9999
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             10042..11079
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(11077..11096)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11121..11169
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        11823..12202
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12203..12406
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     12357..12377
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     12403..12427
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gccaccatgc caatgggtag tcttcagcct ctcgccacct tgtatctgct cggtatgctg
       61 gttgccagcg ttctggctaa cttgtgggtg accgtatact acggggtccc ggtatggaag
      121 gaggcaaaca ctacactctt ctgtgcttcc gacgcaaagg ggtatgatac ggagaagcat
      181 aatgtctggg cgacacatgc atgtgtgcct acagacccaa acccgcagga agtcctcctt
      241 ggtaataacg tgaccgagaa ctttaatatg tggaaaaata acatggtaga gcagatgcat
      301 gaagacatta taagcttgtg ggatcaatct cttaagccgt gtgtgaagct gacaccgctt
      361 tgcgtaacac tcaattgtac ggatttgacg aaaaatagca ctaccaataa ctccaccgct
      421 actaacaata ccacgaacaa tagtaccgaa aaaatggaga tggacaaagg cgagatgaag
      481 aattgctcct ttaatatcac aacaaatatt cgagataaga tgcagaaaga atacgctttg
      541 ctgtacaagc ttgacatcgt gccgatcgac aatggcaaga ataacagcaa caccaattat
      601 cgcctgattt catgtaatac ctcagagatt acgcaatcat gtcccaaagt gtcatttcag
      661 cccattccta ttcattattg tgcacccgcc ggttttgcaa tacttaagtg taataataag
      721 acctttaatg gaagcgggcc ttgtactaat gtgtcttccg tgcaatgcac tcacggtatc
      781 cgacctgttg tttcaaccca gcttttgctg aacggaagtc tcgctgaaga agaaatcgtg
      841 attaggtccg ctaacttctc tgacaacacg aagactataa ttgtccaact taaggaagcg
      901 gtcgaaataa actgtacgcg cccgaataat aacacgcgaa aatcaattaa catcgggcct
      961 gggcgagctt tttatgctac tggcaacata ataggggaca tccgccaagc tcattgtaac
     1021 atatcacgga caaagtggaa taatacgttg acagaaattg ctaagaagct gaacaagcag
     1081 tacgagaata gaacaatagc atttaatcaa tcctccggag gcgatcttga gatcgtcatg
     1141 cattcattca actgcggtgg agaatttttc tattgcaaca cttcccagct gttcagagat
     1201 acgtggaaca acacttggaa cggcatatgg aacaacgata cggaggtcaa cgagactatc
     1261 acacttccgt gtagaatcaa acaaataatc aatatgtggc aagaagtcgg gaaggcgatg
     1321 tatgccccgc caatccgcgg ccaaatcaat tgctcatcaa atattacggg actcctgctc
     1381 acccgcgacg ggggagaaag caacaagacc atagaggtct tccggcccgg tggcggagac
     1441 atgagggaca actggaggtc cgagctgtat aagtacaaag ttgttaagat agagcccctc
     1501 ggggttgctc ccaccaaagc taaaagaaga gtggtgcaac gagagaaaag agccgtcggc
     1561 ttgggtgccc tcttcgtcgg atttttggga gctgcaggta gcacaatggg tgctgcctct
     1621 ataacgctga ccgtacaagc tcgacagctt cttagtggta tcgttcaaca acaaaacaat
     1681 ctcttgcgag caatagaagc ccaacagcat ttgcttcaac tgacagtatg gggcgttaag
     1741 cagttgcaag cgcgggtctt ggcggtcgaa cgatacctga aagaccagca actcctgggc
     1801 ttgtggggat gcagcggcaa acttatatgt accacagctg taccttggaa caccagctgg
     1861 agtaacaaga gttacgatta tatctgggac aacatgacgt ggatgcaatg ggagcgcgag
     1921 attgataact atacaggagt tatctacaag ctgattgagg aatcccagaa ccaacaggag
     1981 aaaaatgaac aagaactttt ggccctggat aaatgggcaa atctgtggaa ctggtttgac
     2041 atcacaagat ggctttggta cattaagatt tttattatga tcgtaggggg tttggtaggg
     2101 cttcggatcg tttttgctgt gctctccatc gtgaaccgag tcaggcaggg gtactcacct
     2161 ttgagcctgc aaacgcactt tcctgcgcca agaggaccgg atcgaccaga gggaacggag
     2221 gaggaaggtg gtgaccggga tcgggatcgc agcactcccc tcgtggatgg gttcctttga
     2281 taactcgagg ccggcaaggc cggatccaga catgataaga tacattgatg agtttggaca
     2341 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     2401 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     2461 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa
     2521 atgtggtatg gctgattatg atccggctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     2581 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     2641 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgagg
     2701 tcgactctag aggatcgatc cccgccccgg acgaactaaa cctgactacg acatctctgc
     2761 cccttcttcg cggggcagtg catgtaatcc cttcagttgg ttggtacaac ttgccaactg
     2821 ggccctgttc cacatgtgac acgggggggg accaaacaca aaggggttct ctgactgtag
     2881 ttgacatcct tataaatgga tgtgcacatt tgccaacact gagtggcttt catcctggag
     2941 cagactttgc agtctgtgga ctgcaacaca acattgcctt tatgtgtaac tcttggctga
     3001 agctcttaca ccaatgctgg gggacatgta cctcccaggg gcccaggaag actacgggag
     3061 gctacaccaa cgtcaatcag aggggcctgt gtagctaccg ataagcggac cctcaagagg
     3121 gcattagcaa tagtgtttat aaggccccct tgttaaccct aaacgggtag catatgcttc
     3181 ccgggtagta gtatatacta tccagactaa ccctaattca atagcatatg ttacccaacg
     3241 ggaagcatat gctatcgaat tagggttagt aaaagggtcc taaggaacag cgatatctcc
     3301 caccccatga gctgtcacgg ttttatttac atggggtcag gattccacga gggtagtgaa
     3361 ccattttagt cacaagggca gtggctgaag atcaaggagc gggcagtgaa ctctcctgaa
     3421 tcttcgcctg cttcttcatt ctccttcgtt tagctaatag aataactgct gagttgtgaa
     3481 cagtaaggtg tatgtgaggt gctcgaaaac aaggtttcag gtgacgcccc cagaataaaa
     3541 tttggacggg gggttcagtg gtggcattgt gctatgacac caatataacc ctcacaaacc
     3601 ccttgggcaa taaatactag tgtaggaatg aaacattctg aatatcttta acaatagaaa
     3661 tccatggggt ggggacaagc cgtaaagact ggatgtccat ctcacacgaa tttatggcta
     3721 tgggcaacac ataatcctag tgcaatatga tactggggtt attaagatgt gtcccaggca
     3781 gggaccaaga caggtgaacc atgttgttac actctatttg taacaagggg aaagagagtg
     3841 gacgccgaca gcagcggact ccactggttg tctctaacac ccccgaaaat taaacggggc
     3901 tccacgccaa tggggcccat aaacaaagac aagtggccac tctttttttt gaaattgtgg
     3961 agtgggggca cgcgtcagcc cccacacgcc gccctgcggt tttggactgt aaaataaggg
     4021 tgtaataact tggctgattg taaccccgct aaccactgcg gtcaaaccac ttgcccacaa
     4081 aaccactaat ggcaccccgg ggaatacctg cataagtagg tgggcgggcc aagatagggg
     4141 cgcgattgct gcgatctgga ggacaaatta cacacacttg cgcctgagcg ccaagcacag
     4201 ggttgttggt cctcatattc acgaggtcgc tgagagcacg gtgggctaat gttgccatgg
     4261 gtagcatata ctacccaaat atctggatag catatgctat cctaatctat atctgggtag
     4321 cataggctat cctaatctat atctgggtag catatgctat cctaatctat atctgggtag
     4381 tatatgctat cctaatttat atctgggtag cataggctat cctaatctat atctgggtag
     4441 catatgctat cctaatctat atctgggtag tatatgctat cctaatctgt atccgggtag
     4501 catatgctat cctaatagag attagggtag tatatgctat cctaatttat atctgggtag
     4561 catatactac ccaaatatct ggatagcata tgctatccta atctatatct gggtagcata
     4621 tgctatccta atctatatct gggtagcata ggctatccta atctatatct gggtagcata
     4681 tgctatccta atctatatct gggtagtata tgctatccta atttatatct gggtagcata
     4741 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     4801 tgctatccta atctgtatcc gggtagcata tgctatcctc atgcatatac agtcagcata
     4861 tgatacccag tagtagagtg ggagtgctat cctttgcata tgccgccacc tcccaagggg
     4921 gcgtgaattt tcgctgcttg tccttttcct gctggttgct cccattctta ggtgaattta
     4981 aggaggccag gctaaagccg tcgcatgtct gattgctcac caggtaaatg tcgctaatgt
     5041 tttccaacgc gagaaggtgt tgagcgcgga gctgagtgac gtgacaacat gggtatgccc
     5101 aattgcccca tgttgggagg acgaaaatgg tgacaagaca gatggccaga aatacaccaa
     5161 cagcacgcat gatgtctact ggggatttat tctttagtgc gggggaatac acggctttta
     5221 atacgattga gggcgtctcc taacaagtta catcactcct gcccttcctc accctcatct
     5281 ccatcacctc cttcatctcc gtcatctccg tcatcaccct ccgcggcagc cccttccacc
     5341 ataggtggaa accagggagg caaatctact ccatcgtcaa agctgcacac agtcaccctg
     5401 atattgcagg taggagcggg ctttgtcata acaaggtcct taatcgcatc cttcaaaacc
     5461 tcagcaaata tatgagtttg taaaaagacc atgaaataac agacaatgga ctcccttagc
     5521 gggccaggtt gtgggccggg tccaggggcc attccaaagg ggagacgact caatggtgta
     5581 agacgacatt gtggaatagc aagggcagtt cctcgcctta ggttgtaaag ggaggtctta
     5641 ctacctccat atacgaacac accggcgacc caagttcctt cgtcggtagt cctttctacg
     5701 tgactcctag ccaggagagc tcttaaacct tctgcaatgt tctcaaattt cgggttggaa
     5761 cctccttgac cacgatgctt tccaaaccac cctccttttt tgcgcctgcc tccatcaccc
     5821 tgaccccggg gtccagtgct tgggccttct cctgggtcat ctgcggggcc ctgctctatc
     5881 gctcccgggg gcacgtcagg ctcaccatct gggccacctt cttggtggta ttcaaaataa
     5941 tcggcttccc ctacagggtg gaaaaatggc cttctacctg gagggggcct gcgcggtgga
     6001 gacccggatg atgatgactg actactggga ctcctgggcc tcttttctcc acgtccacga
     6061 cctctccccc tggctctttc acgacttccc cccctggctc tttcacgtcc tctaccccgg
     6121 cggcctccac tacctcctcg accccggcct ccactacctc ctcgaccccg gcctccactg
     6181 cctcctcgac cccggcctcc acctcctgct cctgcccctc ctgctcctgc ccctcctcct
     6241 gctcctgccc ctcctgcccc tcctgctcct gcccctcctg cccctcctgc tcctgcccct
     6301 cctgcccctc ctgctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctgcccct
     6361 cctcctgctc ctgcccctcc tgcccctcct gctcctgccc ctcctgcccc tcctgctcct
     6421 gcccctcctg cccctcctgc tcctgcccct cctgctcctg cccctcctgc tcctgcccct
     6481 cctgctcctg cccctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctgctcct
     6541 gcccctcctg cccctcctgc ccctcctgct cctgcccctc ctcctgctcc tgcccctcct
     6601 gcccctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctcctgc tcctgcccct
     6661 cctcctgctc ctgcccctcc tgcccctcct gcccctcctc ctgctcctgc ccctcctgcc
     6721 cctcctcctg ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc tgcccctcct
     6781 cctgctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctgcccc tcctgcccct
     6841 cctcctgctc ctgcccctcc tcctgctcct gcccctcctg ctcctgcccc tcccgctcct
     6901 gctcctgctc ctgttccacc gtgggtccct ttgcagccaa tgcaacttgg acgtttttgg
     6961 ggtctccgga caccatctct atgtcttggc cctgatcctg agccgcccgg ggctcctggt
     7021 cttccgcctc ctcgtcctcg tcctcttccc cgtcctcgtc catggttatc accccctctt
     7081 ctttgaggtc cactgccgcc ggagccttct ggtccagatg tgtctccctt ctctcctagg
     7141 ccatttccag gtcctgtacc tggcccctcg tcagacatga ttcacactaa aagagatcaa
     7201 tagacatctt tattagacga cgctcagtga atacagggag tgcagactcc tgccccctcc
     7261 aacagccccc ccaccctcat ccccttcatg gtcgctgtca gacagatcca ggtctgaaaa
     7321 ttccccatcc tccgaaccat cctcgtcctc atcaccaatt actcgcagcc cggaaaactc
     7381 ccgctgaaca tcctcaagat ttgcgtcctg agcctcaagc caggcctcaa attcctcgtc
     7441 cccctttttg ctggacggta gggatgggga ttctcgggac ccctcctctt cctcttcaag
     7501 gtcaccagac agagatgcta ctggggcaac ggaagaaaag ctgggtgcgg cctgtgagga
     7561 tcagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg
     7621 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     7681 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     7741 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     7801 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     7861 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     7921 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     7981 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     8041 tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     8101 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     8161 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     8221 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     8281 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     8341 atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     8401 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     8461 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     8521 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     8581 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     8641 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     8701 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     8761 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     8821 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     8881 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     8941 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     9001 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     9061 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     9121 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     9181 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     9241 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     9301 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     9361 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     9421 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttgaagctgt
     9481 ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg
     9541 ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc
     9601 agcaagacgt agcccagcgc gtcggccccg agatgcgccg cgtgcggctg ctggagatgg
     9661 cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga
     9721 attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cctgcttcat
     9781 ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg gaagaaatat atttgcatgt
     9841 ctttagttct atgatgacac aaaccccgcc cagcgtcttg tcattggcga attcgaacac
     9901 gcagatgcag tcggggcggc gcggtccgag gtccacttcg catattaagg tgacgcgtgt
     9961 ggcctcgaac accgagcgac cctgcagcga cccgcttaac agcgtcaaca gcgtgccgca
    10021 gatcccgggg ggcaatgaga tatgaaaaag cctgaactca ccgcgacgtc tgtcgagaag
    10081 tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa
    10141 tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc
    10201 gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg
    10261 attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg catctcccgc
    10321 cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctgcag
    10381 ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc
    10441 ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg
    10501 attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc
    10561 gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac
    10621 ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg
    10681 gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc
    10741 ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat
    10801 ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa
    10861 ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc
    10921 gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc
    10981 gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc
    11041 agcactcgtc cggatcggga gatgggggag gctaactgaa acacggaagg agacaatacc
    11101 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg ggtgttgggt
    11161 cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga
    11221 gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt
    11281 cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc atagccactg
    11341 gccccgtggg ttagggacgg ggtcccccat ggggaatggt ttatggttcg tgggggttat
    11401 tattttgggc gttgcgtggg gtcaggtcca cgactggact gagcagacag acccatggtt
    11461 tttggatggc ctgggcatgg accgcatgta ctggcgcgac acgaacaccg ggcgtctgtg
    11521 gctgccaaac acccccgacc cccaaaaacc accgcgcgga tttctggcgt gccaagctag
    11581 tcgaccaatt ctcatgtttg acagcttatc atcgcagatc cgggcaacgt tgttgccatt
    11641 gctgcaggcg cagaactggt aggtatggaa gatctataca ttgaatcaat attggcaatt
    11701 agccatatta gtcattggtt atatagcata aatcaatatt ggctattggc cattgcatac
    11761 gttgtatcta tatcataata tgtacattta tattggctca tgtccaatat gaccgccatg
    11821 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
    11881 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
    11941 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
    12001 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
    12061 tcaagtgtat catatgccaa gtccgccccc tattgacgtc aatgacggta aatggcccgc
    12121 ctggcattat gcccagtaca tgaccttacg ggactttcct acttggcagt acatctacgt
    12181 attagtcatc gctattacca tggtgatgcg gttttggcag tacaccaatg ggcgtggata
    12241 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
    12301 ttggcaccaa aatcaacggg actttccaaa atgtcgtaat aaccccgccc cgttgacgca
    12361 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg
    12421 tcagatctct agaagctggg taccagctgc tagc
//
