LOCUS       sequence-235199-       12763 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the B41 HIV-1 isolate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12763)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 12763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-B41gp160
FEATURES             Location/Qualifiers
     source          1..12763
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1586..1597
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     polyA_signal    2612..2746
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2636..2655)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2690..2709
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2889..2911)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      3471..5260
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(5562..7487)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(7922..7940)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8008..8112
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8113..8181,8182..8973)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8331..8350)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9144..9732
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9633..9652
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        10163..10308
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             10351..11388
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(11386..11405)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11430..11478
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        12132..12511
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12512..12715
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     12666..12686
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     12712..12736
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gccaccatgc caatgggtag tcttcagcct ctcgccacct tgtatctgct cggtatgctg
       61 gttgccagcg ttctggctaa cttgtgggtg accgtatact acggggtccc ggtatggaag
      121 gaggcaaaca ctacactctt ctgtgcttcc gacgcaaagg ggtatgatac ggagaagcat
      181 aatgtctggg cgacacatgc atgtgtgcct acagacccaa acccgcagga agtcctcctt
      241 ggtaataacg tgaccgagaa ctttaatatg tggaaaaata acatggtaga gcagatgcat
      301 gaagacatta taagcttgtg ggatcaatct cttaagccgt gtgtgaagct gacaccgctt
      361 tgcgtaacac tcaattgtac ggatttgacg aaaaatagca ctaccaataa ctccaccgct
      421 actaacaata ccacgaacaa tagtaccgaa aaaatggaga tggacaaagg cgagatgaag
      481 aattgctcct ttaatatcac aacaaatatt cgagataaga tgcagaaaga atacgctttg
      541 ctgtacaagc ttgacatcgt gccgatcgac aatggcaaga ataacagcaa caccaattat
      601 cgcctgattt catgtaatac ctcagtaatt acgcaatcat gtcccaaagt gtcatttcag
      661 cccattccta ttcattattg tgcacccgcc ggttttgcaa tacttaagtg taataataag
      721 acctttaatg gaagcgggcc ttgtactaat gtgtcttccg tgcaatgcac tcacggtatc
      781 cgacctgttg tttcaaccca gcttttgctg aacggaagtc tcgctgaaga agaaatcgtg
      841 attaggtccg ctaacttctc tgacaacacg aagactataa ttgtccaact taaggaagcg
      901 gtcgaaataa actgtacgcg cccgaataat aacacgcgaa aatcaattaa catcgggcct
      961 gggcgagctt tttatgctac tggcaacata ataggggaca tccgccaagc tcattgtaac
     1021 atatcacgga caaagtggaa taatacgttg acagaaattg ctaagaagct gaacaagcag
     1081 tacgagaata gaacaatagc atttaatcaa tcctccggag gcgatcttga gatcgtcatg
     1141 cattcattca actgcggtgg agaatttttc tattgcaaca cttcccagct gttcagagat
     1201 acgtggaaca acacttggaa cggcatatgg aacaacgata cggaggtcaa cgagactatc
     1261 acacttccgt gtagaatcaa acaaataatc aatatgtggc aagaagtcgg gaaggcgatg
     1321 tatgccccgc caatccgcgg ccaaatcaat tgctcatcaa atattacggg actcctgctc
     1381 acccgcgacg ggggagaaag caacaagacc atagaggtct tccggcccgg tggcggagac
     1441 atgagggaca actggaggtc cgagctgtat aagtacaaag ttgttaagat agagcccctc
     1501 ggggttgctc ccaccaaagc taaaagaaga gtggtgcaac gagagaaaag agccgtcggc
     1561 ttgggtgccc tcttcgtcgg atttttggga gctgcaggta gcacaatggg tgctgcctct
     1621 ataacgctga ccgtacaagc tcgacagctt cttagtggta tcgttcaaca acaaaacaat
     1681 ctcttgcgag caatagaagc ccaacagcat ttgcttcaac tgacagtatg gggcgttaag
     1741 cagttgcaag cgcgggtctt ggcggtcgaa cgatacctga aagaccagca actcctgggc
     1801 ttgtggggat gcagcggcaa acttatatgt accacagctg taccttggaa caccagctgg
     1861 agtaacaaga gttacgatta tatctgggac aacatgacgt ggatgcaatg ggagcgcgag
     1921 attgataact atacaggagt tatctacaag ctgattgagg aatcccagaa ccaacaggag
     1981 aaaaatgaac aagaactttt ggccctggat aaatgggcaa atctgtggaa ctggtttgac
     2041 atcacaagat ggctttggta cattaagatt tttattatga tcgtaggggg tttggtaggg
     2101 cttcggatcg tttttgctgt gctctccatc gtgaaccgag tcaggcaggg gtactcacct
     2161 ttgagcctgc aaacgcactt tcctgcgcca agaggaccgg atcgaccaga gggaacggag
     2221 gaggaaggtg gtgaccggga tcgggatcgc agcactcccc tcgtggatgg gttccttgca
     2281 ataatttggg tagaccttag gagtttgttt cttttctcct accaccgcct gagagacctc
     2341 ctcctgatag tgacacggat tgtcgaactg cttggtcgca gaggttggga ggcccttaag
     2401 tactggtgga atcttcttca gtattggtcc caagagctta ggaactctgc ggtctccttg
     2461 ttcaatgcca cagcgatcgc agtggctgag ggaaccgacc gagtcattga ggcggtccaa
     2521 agaattggcc gggcaattct tcacatacct cgaagaataa ggcagggctt tgagagagca
     2581 ctgcagtagt aactcgaggc cggcaaggcc ggatccagac atgataagat acattgatga
     2641 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     2701 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     2761 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
     2821 cctctacaaa tgtggtatgg ctgattatga tccggctgcc tcgcgcgttt cggtgatgac
     2881 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     2941 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca
     3001 gccatgaggt cgactctaga ggatcgatcc ccgccccgga cgaactaaac ctgactacga
     3061 catctctgcc ccttcttcgc ggggcagtgc atgtaatccc ttcagttggt tggtacaact
     3121 tgccaactgg gccctgttcc acatgtgaca cgggggggga ccaaacacaa aggggttctc
     3181 tgactgtagt tgacatcctt ataaatggat gtgcacattt gccaacactg agtggctttc
     3241 atcctggagc agactttgca gtctgtggac tgcaacacaa cattgccttt atgtgtaact
     3301 cttggctgaa gctcttacac caatgctggg ggacatgtac ctcccagggg cccaggaaga
     3361 ctacgggagg ctacaccaac gtcaatcaga ggggcctgtg tagctaccga taagcggacc
     3421 ctcaagaggg cattagcaat agtgtttata aggccccctt gttaacccta aacgggtagc
     3481 atatgcttcc cgggtagtag tatatactat ccagactaac cctaattcaa tagcatatgt
     3541 tacccaacgg gaagcatatg ctatcgaatt agggttagta aaagggtcct aaggaacagc
     3601 gatatctccc accccatgag ctgtcacggt tttatttaca tggggtcagg attccacgag
     3661 ggtagtgaac cattttagtc acaagggcag tggctgaaga tcaaggagcg ggcagtgaac
     3721 tctcctgaat cttcgcctgc ttcttcattc tccttcgttt agctaataga ataactgctg
     3781 agttgtgaac agtaaggtgt atgtgaggtg ctcgaaaaca aggtttcagg tgacgccccc
     3841 agaataaaat ttggacgggg ggttcagtgg tggcattgtg ctatgacacc aatataaccc
     3901 tcacaaaccc cttgggcaat aaatactagt gtaggaatga aacattctga atatctttaa
     3961 caatagaaat ccatggggtg gggacaagcc gtaaagactg gatgtccatc tcacacgaat
     4021 ttatggctat gggcaacaca taatcctagt gcaatatgat actggggtta ttaagatgtg
     4081 tcccaggcag ggaccaagac aggtgaacca tgttgttaca ctctatttgt aacaagggga
     4141 aagagagtgg acgccgacag cagcggactc cactggttgt ctctaacacc cccgaaaatt
     4201 aaacggggct ccacgccaat ggggcccata aacaaagaca agtggccact cttttttttg
     4261 aaattgtgga gtgggggcac gcgtcagccc ccacacgccg ccctgcggtt ttggactgta
     4321 aaataagggt gtaataactt ggctgattgt aaccccgcta accactgcgg tcaaaccact
     4381 tgcccacaaa accactaatg gcaccccggg gaatacctgc ataagtaggt gggcgggcca
     4441 agataggggc gcgattgctg cgatctggag gacaaattac acacacttgc gcctgagcgc
     4501 caagcacagg gttgttggtc ctcatattca cgaggtcgct gagagcacgg tgggctaatg
     4561 ttgccatggg tagcatatac tacccaaata tctggatagc atatgctatc ctaatctata
     4621 tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata
     4681 tctgggtagt atatgctatc ctaatttata tctgggtagc ataggctatc ctaatctata
     4741 tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc ctaatctgta
     4801 tccgggtagc atatgctatc ctaatagaga ttagggtagt atatgctatc ctaatttata
     4861 tctgggtagc atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg
     4921 ggtagcatat gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg
     4981 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tttatatctg
     5041 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     5101 ggtagtatat gctatcctaa tctgtatccg ggtagcatat gctatcctca tgcatataca
     5161 gtcagcatat gatacccagt agtagagtgg gagtgctatc ctttgcatat gccgccacct
     5221 cccaaggggg cgtgaatttt cgctgcttgt ccttttcctg ctggttgctc ccattcttag
     5281 gtgaatttaa ggaggccagg ctaaagccgt cgcatgtctg attgctcacc aggtaaatgt
     5341 cgctaatgtt ttccaacgcg agaaggtgtt gagcgcggag ctgagtgacg tgacaacatg
     5401 ggtatgccca attgccccat gttgggagga cgaaaatggt gacaagacag atggccagaa
     5461 atacaccaac agcacgcatg atgtctactg gggatttatt ctttagtgcg ggggaataca
     5521 cggcttttaa tacgattgag ggcgtctcct aacaagttac atcactcctg cccttcctca
     5581 ccctcatctc catcacctcc ttcatctccg tcatctccgt catcaccctc cgcggcagcc
     5641 ccttccacca taggtggaaa ccagggaggc aaatctactc catcgtcaaa gctgcacaca
     5701 gtcaccctga tattgcaggt aggagcgggc tttgtcataa caaggtcctt aatcgcatcc
     5761 ttcaaaacct cagcaaatat atgagtttgt aaaaagacca tgaaataaca gacaatggac
     5821 tcccttagcg ggccaggttg tgggccgggt ccaggggcca ttccaaaggg gagacgactc
     5881 aatggtgtaa gacgacattg tggaatagca agggcagttc ctcgccttag gttgtaaagg
     5941 gaggtcttac tacctccata tacgaacaca ccggcgaccc aagttccttc gtcggtagtc
     6001 ctttctacgt gactcctagc caggagagct cttaaacctt ctgcaatgtt ctcaaatttc
     6061 gggttggaac ctccttgacc acgatgcttt ccaaaccacc ctcctttttt gcgcctgcct
     6121 ccatcaccct gaccccgggg tccagtgctt gggccttctc ctgggtcatc tgcggggccc
     6181 tgctctatcg ctcccggggg cacgtcaggc tcaccatctg ggccaccttc ttggtggtat
     6241 tcaaaataat cggcttcccc tacagggtgg aaaaatggcc ttctacctgg agggggcctg
     6301 cgcggtggag acccggatga tgatgactga ctactgggac tcctgggcct cttttctcca
     6361 cgtccacgac ctctccccct ggctctttca cgacttcccc ccctggctct ttcacgtcct
     6421 ctaccccggc ggcctccact acctcctcga ccccggcctc cactacctcc tcgaccccgg
     6481 cctccactgc ctcctcgacc ccggcctcca cctcctgctc ctgcccctcc tgctcctgcc
     6541 cctcctcctg ctcctgcccc tcctgcccct cctgctcctg cccctcctgc ccctcctgct
     6601 cctgcccctc ctgcccctcc tgctcctgcc cctcctgccc ctcctcctgc tcctgcccct
     6661 cctgcccctc ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc tcctgcccct
     6721 cctgctcctg cccctcctgc ccctcctgct cctgcccctc ctgctcctgc ccctcctgct
     6781 cctgcccctc ctgctcctgc ccctcctgcc cctcctgccc ctcctcctgc tcctgcccct
     6841 cctgctcctg cccctcctgc ccctcctgcc cctcctgctc ctgcccctcc tcctgctcct
     6901 gcccctcctg cccctcctgc ccctcctcct gctcctgccc ctcctgcccc tcctcctgct
     6961 cctgcccctc ctcctgctcc tgcccctcct gcccctcctg cccctcctcc tgctcctgcc
     7021 cctcctgccc ctcctcctgc tcctgcccct cctcctgctc ctgcccctcc tgcccctcct
     7081 gcccctcctc ctgctcctgc ccctcctcct gctcctgccc ctcctgcccc tcctgcccct
     7141 cctgcccctc ctcctgctcc tgcccctcct cctgctcctg cccctcctgc tcctgcccct
     7201 cccgctcctg ctcctgctcc tgttccaccg tgggtccctt tgcagccaat gcaacttgga
     7261 cgtttttggg gtctccggac accatctcta tgtcttggcc ctgatcctga gccgcccggg
     7321 gctcctggtc ttccgcctcc tcgtcctcgt cctcttcccc gtcctcgtcc atggttatca
     7381 ccccctcttc tttgaggtcc actgccgccg gagccttctg gtccagatgt gtctcccttc
     7441 tctcctaggc catttccagg tcctgtacct ggcccctcgt cagacatgat tcacactaaa
     7501 agagatcaat agacatcttt attagacgac gctcagtgaa tacagggagt gcagactcct
     7561 gccccctcca acagcccccc caccctcatc cccttcatgg tcgctgtcag acagatccag
     7621 gtctgaaaat tccccatcct ccgaaccatc ctcgtcctca tcaccaatta ctcgcagccc
     7681 ggaaaactcc cgctgaacat cctcaagatt tgcgtcctga gcctcaagcc aggcctcaaa
     7741 ttcctcgtcc ccctttttgc tggacggtag ggatggggat tctcgggacc cctcctcttc
     7801 ctcttcaagg tcaccagaca gagatgctac tggggcaacg gaagaaaagc tgggtgcggc
     7861 ctgtgaggat cagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag
     7921 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     7981 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     8041 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     8101 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     8161 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     8221 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     8281 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     8341 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     8401 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     8461 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     8521 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     8581 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     8641 gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     8701 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     8761 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     8821 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     8881 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     8941 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     9001 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     9061 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     9121 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     9181 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     9241 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     9301 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     9361 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     9421 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     9481 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     9541 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     9601 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     9661 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     9721 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     9781 tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc
     9841 atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc
     9901 gcgaacgcca gcaagacgta gcccagcgcg tcggccccga gatgcgccgc gtgcggctgc
     9961 tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc
    10021 tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc
    10081 ctgcttcatc cccgtggccc gttgctcgcg tttgctggcg gtgtccccgg aagaaatata
    10141 tttgcatgtc tttagttcta tgatgacaca aaccccgccc agcgtcttgt cattggcgaa
    10201 ttcgaacacg cagatgcagt cggggcggcg cggtccgagg tccacttcgc atattaaggt
    10261 gacgcgtgtg gcctcgaaca ccgagcgacc ctgcagcgac ccgcttaaca gcgtcaacag
    10321 cgtgccgcag atcccggggg gcaatgagat atgaaaaagc ctgaactcac cgcgacgtct
    10381 gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag
    10441 ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta
    10501 aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc
    10561 gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc
    10621 atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct
    10681 gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg
    10741 agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc
    10801 atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc
    10861 agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa
    10921 gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc
    10981 ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc
    11041 aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag
    11101 cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt
    11161 cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag
    11221 ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc
    11281 cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac
    11341 cgacgcccca gcactcgtcc ggatcgggag atgggggagg ctaactgaaa cacggaagga
    11401 gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg
    11461 gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata
    11521 ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc
    11581 ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca
    11641 tagccactgg ccccgtgggt tagggacggg gtcccccatg gggaatggtt tatggttcgt
    11701 gggggttatt attttgggcg ttgcgtgggg tcaggtccac gactggactg agcagacaga
    11761 cccatggttt ttggatggcc tgggcatgga ccgcatgtac tggcgcgaca cgaacaccgg
    11821 gcgtctgtgg ctgccaaaca cccccgaccc ccaaaaacca ccgcgcggat ttctggcgtg
    11881 ccaagctagt cgaccaattc tcatgtttga cagcttatca tcgcagatcc gggcaacgtt
    11941 gttgccattg ctgcaggcgc agaactggta ggtatggaag atctatacat tgaatcaata
    12001 ttggcaatta gccatattag tcattggtta tatagcataa atcaatattg gctattggcc
    12061 attgcatacg ttgtatctat atcataatat gtacatttat attggctcat gtccaatatg
    12121 accgccatgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
    12181 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
    12241 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
    12301 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
    12361 ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca atgacggtaa
    12421 atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta cttggcagta
    12481 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acaccaatgg
    12541 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
    12601 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata accccgcccc
    12661 gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt
    12721 agtgaaccgt cagatctcta gaagctgggt accagctgct agc
//
