LOCUS       sequence-23520-e         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Zhou BY, Ye Z, Chen G, Gao ZP, Zhang YA, Cheng L
  TITLE     Inducible and reversible transgene expression in human stem cells 
            after efficient and stable gene transfer.
  JOURNAL   Stem Cells. 2007 Mar . 25(3):779-89.
  PUBMED    17158240
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cells.
            2007 Mar . 25(3):779-89."
COMMENT     Sequence Label: iDuet011a
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      16..25
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(217..236)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
ORIGIN
        1 gctctgccct ngctcccatg gtggcgaccg gtacccgggg atcctctaga gtcgacctgc
       61 agcccaagct tcgtctaaca aaaaagccaa aaacggccag aatttagcgg acaatttact
      121 agtctaacac tgaaaattac atattgaccc aaatgattac atttcaaaag gtgcctaaaa
      181 aacttcacaa aacacactcg ccaaccccga gcgcatagtt caaaaccgga gcttcagcta
      241 cttaagaaga taggtacata aaaccgacca aagaaactga cgcctcactt atccctcccc
      301 tcaccagagg tccggcgcct gtcgattcag gagagcctac cctaggcccg aaccctgcgt
      361 cctgcgacgg agaaaagcct accgcacacc taccggcagg tggccccacc ctgcattata
      421 agccaacaga acgggtgacg tcacgacacg acgagggcgc gcgctcccaa aggtacgggt
      481 gcactgccca acggcaccgc cataactgcc gcccccgcaa cagacgacaa accgagttct
      541 ccagtcagtg acaaacttca cgtcagggtc cccagatggt gccccagccc atctcacccg
      601 aataagagct ttcccgcatt agcgaaggcc tcaagacctt gggttcttgc cgcccaccat
      661 gccccccacc ttgtttcaac gacctcacag cccgcctcac aagcgtcttc cattcaagac
      721 tcgggaacag ccgccatttt gctgcgctcc ccccaacccc cagttcaggg caaccttgct
      781 cgcggaccag actacagcct tgggggtttc tccacacgct ncgtccaccg aggggccggg
      841 gccacnaagc ccggcag
//
