LOCUS       sequence-235205-       12736 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the DU422 HIV-1 isolate; 
            QES mutant with enhanced binding to PG16.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12736)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 12736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-DU422-QES.c12
FEATURES             Location/Qualifiers
     source          1..12736
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    2585..2719
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2609..2628)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2663..2682
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2862..2884)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      3444..5233
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(5535..7460)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(7895..7913)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7981..8085
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8086..8154,8155..8946)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8304..8323)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9117..9705
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9606..9625
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        10136..10281
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             10324..11361
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(11359..11378)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11403..11451
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        12105..12484
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12485..12688
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     12639..12659
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     12685..12709
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gccaccatgc ccatgggttc cctccaacca cttgcaacgc tttatctgct cggcatgctt
       61 gtagcgtcag tgcttgcaaa tctcgacctt tgggtcaccg tatactacgg agtccccgtg
      121 tggaaagagg ctaagacgac cctgttttgt gcgtcagacg cgaaagcgta tgacaaggaa
      181 gtgcataacg tgtgggcaac acacgcatgt gttccgacgg accctaaccc gcaggaaatc
      241 gtccttgaaa acgtgaccga aaacttcaac atgtggaaga acgacatggt tgaccaaatg
      301 cacgaggaca taatcagtct ttgggatcaa tccttgaagc cctgtgtgaa gctgacgcct
      361 ttgtgcgtaa ctttgaactg taaaaacgta aatatttcag cgaatgctaa cgcgaccgct
      421 acacttaatt caagtatgaa cggcgaaata aagaattgct ccttcaacac cacgacagaa
      481 cttagagata aaaagcaaaa agtctacgcc cttttctata aaccggatct cgtgccactt
      541 aacggtggtg agcacaatga gacaggtgag tacattctca tcaactgcaa ttcatctaca
      601 ataacgcaag catgtccgaa aatgtccttc gatccgatcc ctattcacta ctgcgctcct
      661 gcagggtacg ccatactgaa gtgtaacaat aaaacgttta acggaacagg cccttgcaat
      721 aatgtaagta ctgtccagtg cacgcatggc ataaaaccca ccatgagcac acaacttctt
      781 cttaacggat cactcgccga ggaagagatc atcgtgcgaa gtgagaactt gacgaataat
      841 ataaagccga tcatagtcca tctcaacaag tccgtcgaga ttaaatgcac taggcccaac
      901 aacaatacgc ggaaatccgt acggataggt cctggacaaa cattctatgc taccggtgag
      961 attataggtg atatacgcga agctcattgc aatatcagta gggagacctg gaattccact
     1021 ttgatccaag tgaaagaaaa actgcgggag cattacaaca aaacgatcaa atttgaacct
     1081 tcttctggag gtgacctgga ggttacgacg cacagtttta actgtagggg agaatggttc
     1141 tactgcgata cgaccaagct gttcaacgag actaagctgt ttaacgagtc agaatacgtt
     1201 gacaataaga ctattatctt gccctgccgg ataaagcaaa ttatcaatat gtggcaagaa
     1261 gtgggaaggg caatgtacgc gccgccgatt gaagggaata ttacttgcaa gagcaacata
     1321 actggtcttc ttctcacatg ggatggtgga gagaacagta ccgaaggcgt ttttaggcct
     1381 ggcggtggca atatgaagga taactggcgc tcagaactct ggaagtacaa agtcgtcgaa
     1441 ataaagccct tgggcgttgc gcctacgaaa agtaaacgaa aagtagtagg gagggaaaag
     1501 cgagcggttg gcttgggtgc agtgcttctc gggttcctgg gagctgcggg gtcaacaatg
     1561 ggagctgctt ccataacctt gactgtgcag gcaaggcagc tgctgagtgg gatagtgcag
     1621 caacaatcca atctgcttag ggcaattgaa gctcagcaac atcttttgca gctcacagtc
     1681 tggggcataa agcaactgca aaccagagtc ctcgcgattg agcggtatct taaggaccag
     1741 caactgctgg gactctgggg gtgttccggg aagcttattt gtgccactgc ggtaccatgg
     1801 aactcctctt ggtccaacaa gtctctgggc gatatttggg acaatatgac gtggatgcag
     1861 tgggatcgcg agatatccaa ctacaccaac acgatcttcc ggcttctcga agacagtcag
     1921 aatcaacaag agaagaatga gaaggatctg ttggcccttg acagctggaa aaatctttgg
     1981 aattggtttg acattactaa ctggctgtgg tacataaaaa tcttcataat gattgtcggt
     2041 ggccttatcg gtctccgcat catattcggg gtattggcta tagtcaaaag ggtacgccag
     2101 gggtatagcc cgttgagttt ccaaacgctc attccgaatc cgcgaggtcc tgatcgcttg
     2161 ggacggatcg aagaggaagg aggtgaacaa gacaaggatc gatccatacg actggtctct
     2221 gggtttcttg ccctcgcgtg ggacgacctc agatcactgt gcctgttctc ttatcatcaa
     2281 ttgagggact tcatactgac cgcagcaaga gccgcagagc tgctgggtcg cagttccctg
     2341 cgaggtcttc agcgaggttg ggaagtactc aagtacctcg gaaatctggt ccaatactgg
     2401 ggtctcgaac tgaagcgatc cgcgataaac ctctttgaca caatagcaat tgcggtggcc
     2461 gagggaacgg accgaataat agaagtcata cagcgcattt gcagggctat ccgatatatc
     2521 ccaacccgaa ttcgacaagg tttcgaagcc gcattgttgt agtaactcga ggccggcaag
     2581 gccggatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     2641 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     2701 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     2761 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta
     2821 tgatccggct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     2881 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     2941 gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga ggtcgactct agaggatcga
     3001 tccccgcccc ggacgaacta aacctgacta cgacatctct gccccttctt cgcggggcag
     3061 tgcatgtaat cccttcagtt ggttggtaca acttgccaac tgggccctgt tccacatgtg
     3121 acacgggggg ggaccaaaca caaaggggtt ctctgactgt agttgacatc cttataaatg
     3181 gatgtgcaca tttgccaaca ctgagtggct ttcatcctgg agcagacttt gcagtctgtg
     3241 gactgcaaca caacattgcc tttatgtgta actcttggct gaagctctta caccaatgct
     3301 gggggacatg tacctcccag gggcccagga agactacggg aggctacacc aacgtcaatc
     3361 agaggggcct gtgtagctac cgataagcgg accctcaaga gggcattagc aatagtgttt
     3421 ataaggcccc cttgttaacc ctaaacgggt agcatatgct tcccgggtag tagtatatac
     3481 tatccagact aaccctaatt caatagcata tgttacccaa cgggaagcat atgctatcga
     3541 attagggtta gtaaaagggt cctaaggaac agcgatatct cccaccccat gagctgtcac
     3601 ggttttattt acatggggtc aggattccac gagggtagtg aaccatttta gtcacaaggg
     3661 cagtggctga agatcaagga gcgggcagtg aactctcctg aatcttcgcc tgcttcttca
     3721 ttctccttcg tttagctaat agaataactg ctgagttgtg aacagtaagg tgtatgtgag
     3781 gtgctcgaaa acaaggtttc aggtgacgcc cccagaataa aatttggacg gggggttcag
     3841 tggtggcatt gtgctatgac accaatataa ccctcacaaa ccccttgggc aataaatact
     3901 agtgtaggaa tgaaacattc tgaatatctt taacaataga aatccatggg gtggggacaa
     3961 gccgtaaaga ctggatgtcc atctcacacg aatttatggc tatgggcaac acataatcct
     4021 agtgcaatat gatactgggg ttattaagat gtgtcccagg cagggaccaa gacaggtgaa
     4081 ccatgttgtt acactctatt tgtaacaagg ggaaagagag tggacgccga cagcagcgga
     4141 ctccactggt tgtctctaac acccccgaaa attaaacggg gctccacgcc aatggggccc
     4201 ataaacaaag acaagtggcc actctttttt ttgaaattgt ggagtggggg cacgcgtcag
     4261 cccccacacg ccgccctgcg gttttggact gtaaaataag ggtgtaataa cttggctgat
     4321 tgtaaccccg ctaaccactg cggtcaaacc acttgcccac aaaaccacta atggcacccc
     4381 ggggaatacc tgcataagta ggtgggcggg ccaagatagg ggcgcgattg ctgcgatctg
     4441 gaggacaaat tacacacact tgcgcctgag cgccaagcac agggttgttg gtcctcatat
     4501 tcacgaggtc gctgagagca cggtgggcta atgttgccat gggtagcata tactacccaa
     4561 atatctggat agcatatgct atcctaatct atatctgggt agcataggct atcctaatct
     4621 atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt
     4681 atatctgggt agcataggct atcctaatct atatctgggt agcatatgct atcctaatct
     4741 atatctgggt agtatatgct atcctaatct gtatccgggt agcatatgct atcctaatag
     4801 agattagggt agtatatgct atcctaattt atatctgggt agcatatact acccaaatat
     4861 ctggatagca tatgctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     4921 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     4981 ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat
     5041 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat
     5101 ccgggtagca tatgctatcc tcatgcatat acagtcagca tatgataccc agtagtagag
     5161 tgggagtgct atcctttgca tatgccgcca cctcccaagg gggcgtgaat tttcgctgct
     5221 tgtccttttc ctgctggttg ctcccattct taggtgaatt taaggaggcc aggctaaagc
     5281 cgtcgcatgt ctgattgctc accaggtaaa tgtcgctaat gttttccaac gcgagaaggt
     5341 gttgagcgcg gagctgagtg acgtgacaac atgggtatgc ccaattgccc catgttggga
     5401 ggacgaaaat ggtgacaaga cagatggcca gaaatacacc aacagcacgc atgatgtcta
     5461 ctggggattt attctttagt gcgggggaat acacggcttt taatacgatt gagggcgtct
     5521 cctaacaagt tacatcactc ctgcccttcc tcaccctcat ctccatcacc tccttcatct
     5581 ccgtcatctc cgtcatcacc ctccgcggca gccccttcca ccataggtgg aaaccaggga
     5641 ggcaaatcta ctccatcgtc aaagctgcac acagtcaccc tgatattgca ggtaggagcg
     5701 ggctttgtca taacaaggtc cttaatcgca tccttcaaaa cctcagcaaa tatatgagtt
     5761 tgtaaaaaga ccatgaaata acagacaatg gactccctta gcgggccagg ttgtgggccg
     5821 ggtccagggg ccattccaaa ggggagacga ctcaatggtg taagacgaca ttgtggaata
     5881 gcaagggcag ttcctcgcct taggttgtaa agggaggtct tactacctcc atatacgaac
     5941 acaccggcga cccaagttcc ttcgtcggta gtcctttcta cgtgactcct agccaggaga
     6001 gctcttaaac cttctgcaat gttctcaaat ttcgggttgg aacctccttg accacgatgc
     6061 tttccaaacc accctccttt tttgcgcctg cctccatcac cctgaccccg gggtccagtg
     6121 cttgggcctt ctcctgggtc atctgcgggg ccctgctcta tcgctcccgg gggcacgtca
     6181 ggctcaccat ctgggccacc ttcttggtgg tattcaaaat aatcggcttc ccctacaggg
     6241 tggaaaaatg gccttctacc tggagggggc ctgcgcggtg gagacccgga tgatgatgac
     6301 tgactactgg gactcctggg cctcttttct ccacgtccac gacctctccc cctggctctt
     6361 tcacgacttc cccccctggc tctttcacgt cctctacccc ggcggcctcc actacctcct
     6421 cgaccccggc ctccactacc tcctcgaccc cggcctccac tgcctcctcg accccggcct
     6481 ccacctcctg ctcctgcccc tcctgctcct gcccctcctc ctgctcctgc ccctcctgcc
     6541 cctcctgctc ctgcccctcc tgcccctcct gctcctgccc ctcctgcccc tcctgctcct
     6601 gcccctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctcctgc tcctgcccct
     6661 cctgcccctc ctgctcctgc ccctcctgcc cctcctgctc ctgcccctcc tgcccctcct
     6721 gctcctgccc ctcctgctcc tgcccctcct gctcctgccc ctcctgctcc tgcccctcct
     6781 gcccctcctg cccctcctcc tgctcctgcc cctcctgctc ctgcccctcc tgcccctcct
     6841 gcccctcctg ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc tgcccctcct
     6901 cctgctcctg cccctcctgc ccctcctcct gctcctgccc ctcctcctgc tcctgcccct
     6961 cctgcccctc ctgcccctcc tcctgctcct gcccctcctg cccctcctcc tgctcctgcc
     7021 cctcctcctg ctcctgcccc tcctgcccct cctgcccctc ctcctgctcc tgcccctcct
     7081 cctgctcctg cccctcctgc ccctcctgcc cctcctgccc ctcctcctgc tcctgcccct
     7141 cctcctgctc ctgcccctcc tgctcctgcc cctcccgctc ctgctcctgc tcctgttcca
     7201 ccgtgggtcc ctttgcagcc aatgcaactt ggacgttttt ggggtctccg gacaccatct
     7261 ctatgtcttg gccctgatcc tgagccgccc ggggctcctg gtcttccgcc tcctcgtcct
     7321 cgtcctcttc cccgtcctcg tccatggtta tcaccccctc ttctttgagg tccactgccg
     7381 ccggagcctt ctggtccaga tgtgtctccc ttctctccta ggccatttcc aggtcctgta
     7441 cctggcccct cgtcagacat gattcacact aaaagagatc aatagacatc tttattagac
     7501 gacgctcagt gaatacaggg agtgcagact cctgccccct ccaacagccc ccccaccctc
     7561 atccccttca tggtcgctgt cagacagatc caggtctgaa aattccccat cctccgaacc
     7621 atcctcgtcc tcatcaccaa ttactcgcag cccggaaaac tcccgctgaa catcctcaag
     7681 atttgcgtcc tgagcctcaa gccaggcctc aaattcctcg tccccctttt tgctggacgg
     7741 tagggatggg gattctcggg acccctcctc ttcctcttca aggtcaccag acagagatgc
     7801 tactggggca acggaagaaa agctgggtgc ggcctgtgag gatcagctta tcgatgataa
     7861 gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag
     7921 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     7981 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     8041 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     8101 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     8161 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     8221 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     8281 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg
     8341 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     8401 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     8461 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     8521 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     8581 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca
     8641 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     8701 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     8761 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     8821 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     8881 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     8941 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     9001 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     9061 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     9121 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     9181 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     9241 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     9301 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     9361 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     9421 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     9481 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     9541 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     9601 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     9661 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     9721 gcctttttac ggttcctggc cttttgctgg ccttgaagct gtccctgatg gtcgtcatct
     9781 acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga
     9841 atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc
     9901 gcgtcggccc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt
     9961 tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc
    10021 ttggagtggt gaatccgtta gcgaggtgcc gccctgcttc atccccgtgg cccgttgctc
    10081 gcgtttgctg gcggtgtccc cggaagaaat atatttgcat gtctttagtt ctatgatgac
    10141 acaaaccccg cccagcgtct tgtcattggc gaattcgaac acgcagatgc agtcggggcg
    10201 gcgcggtccg aggtccactt cgcatattaa ggtgacgcgt gtggcctcga acaccgagcg
    10261 accctgcagc gacccgctta acagcgtcaa cagcgtgccg cagatcccgg ggggcaatga
    10321 gatatgaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc
    10381 gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc
    10441 gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa
    10501 gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac
    10561 attggggaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg
    10621 ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg
    10681 gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa
    10741 ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg
    10801 tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat
    10861 gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc
    10921 ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag
    10981 gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg
    11041 gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg
    11101 ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt
    11161 gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc
    11221 ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat
    11281 ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccggatcgg
    11341 gagatggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat
    11401 gacggcaata aaaagacaga ataaaacgca cgggtgttgg gtcgtttgtt cataaacgcg
    11461 gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat
    11521 acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc
    11581 tcgcagccaa cgtcggggcg gcaggccctg ccatagccac tggccccgtg ggttagggac
    11641 ggggtccccc atggggaatg gtttatggtt cgtgggggtt attattttgg gcgttgcgtg
    11701 gggtcaggtc cacgactgga ctgagcagac agacccatgg tttttggatg gcctgggcat
    11761 ggaccgcatg tactggcgcg acacgaacac cgggcgtctg tggctgccaa acacccccga
    11821 cccccaaaaa ccaccgcgcg gatttctggc gtgccaagct agtcgaccaa ttctcatgtt
    11881 tgacagctta tcatcgcaga tccgggcaac gttgttgcca ttgctgcagg cgcagaactg
    11941 gtaggtatgg aagatctata cattgaatca atattggcaa ttagccatat tagtcattgg
    12001 ttatatagca taaatcaata ttggctattg gccattgcat acgttgtatc tatatcataa
    12061 tatgtacatt tatattggct catgtccaat atgaccgcca tgttgacatt gattattgac
    12121 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
    12181 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
    12241 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
    12301 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
    12361 aagtccgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
    12421 catgacctta cgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
    12481 catggtgatg cggttttggc agtacaccaa tgggcgtgga tagcggtttg actcacgggg
    12541 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
    12601 ggactttcca aaatgtcgta ataaccccgc cccgttgacg caaatgggcg gtaggcgtgt
    12661 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatct ctagaagctg
    12721 ggtaccagct gctagc
//
