LOCUS       sequence-235207-       12328 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for soluble gp140 from the DU422 HIV-1 
            isolate with a transmembrane tether.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12328)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 12328)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-DU422gp140-TM
FEATURES             Location/Qualifiers
     source          1..12328
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2017..2034
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2177..2311
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2201..2220)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2255..2274
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2454..2476)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      3036..4825
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(5127..7052)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(7487..7505)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7573..7677
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7678..7746,7747..8538)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7896..7915)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8709..9297
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9198..9217
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        9728..9873
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             9916..10953
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(10951..10970)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10995..11043
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        11697..12076
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12077..12280
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     12231..12251
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     12277..12301
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gccaccatgc ccatgggttc cctccaacca cttgcaacgc tttatctgct cggcatgctt
       61 gtagcgtcag tgcttgcaaa tctcgacctt tgggtcaccg tatactacgg agtccccgtg
      121 tggaaagagg ctaagacgac cctgttttgt gcgtcagacg cgaaagcgta tgacaaggaa
      181 gtgcataacg tgtgggcaac acacgcatgt gttccgacgg accctaaccc gcaggaaatc
      241 gtccttgaaa acgtgaccga aaacttcaac atgtggaaga acgacatggt tgaccaaatg
      301 cacgaggaca taatcagtct ttgggatcaa tccttgaagc cctgtgtgaa gctgacgcct
      361 ttgtgcgtaa ctttgaactg taaaaacgta aatatttcag cgaatgctaa cgcgaccgct
      421 acacttaatt caagtatgaa cggcgaaata aagaattgct ccttcaacac cacgacagaa
      481 cttagagata aaaagcaaaa agtctacgcc cttttctata aaccggatgt cgtgccactt
      541 aacggtggtg agcacaatga gacaggtgag tacattctca tcaactgcaa ttcatctaca
      601 ataacgcaag catgtccgaa agtttccttc gatccgatcc ctattcacta ctgcgctcct
      661 gcagggtacg ccatactgaa gtgtaacaat aaaacgttta acggaacagg cccttgcaat
      721 aatgtaagta ctgtccagtg cacgcatggc ataaaacccg tcgtgagcac acaacttctt
      781 cttaacggat cactcgccga ggaagagatc atcgtgcgaa gtgagaactt gacgaataat
      841 ataaagacga tcatagtcca tctcaacaag tccgtcgaga ttaaatgcac taggcccaac
      901 aacaatacgc ggaaatccgt acggataggt cctggacaaa cattctatgc taccggtgag
      961 attataggtg atatacgcga agctcattgc aatatcagta gggagacctg gaattccact
     1021 ttgatccaag tgaaagaaaa actgcgggag cattacaaca aaacgatcaa atttgaacct
     1081 tcttctggag gtgacctgga ggttacgacg cacagtttta actgtagggg agaattcttc
     1141 tactgcgata cgaccaagct gttcaacgag actaagctgt ttaacgagtc agaatacgtt
     1201 gacaataaga ctattatctt gccctgccgg ataaagcaaa ttatcaatat gtggcaagaa
     1261 gtgggaaggg caatgtacgc gccgccgatt gaagggaata ttacttgcaa gagcaacata
     1321 actggtcttc ttctcacatg ggatggtgga gagaacagta ccgaaggcgt ttttaggcct
     1381 ggcggtggca atatgaagga taactggcgc tcagaactct ataagtacaa agtcgtcgaa
     1441 ataaagccct tgggcgttgc gcctacgaaa agtaaacgaa aagtagtagg gagggaaaag
     1501 cgagcggttg gcttgggtgc agtgcttctc gggttcctgg gagctgcggg gtcaacaatg
     1561 ggagctgctt ccataacctt gactgtgcag gcaaggcagc tgctgagtgg gatagtgcag
     1621 caacaatcca atctgcttag ggcaattgaa gctcagcaac atcttttgca gctcacagtc
     1681 tggggcataa agcaactgca aaccagagtc ctcgcgattg agcggtatct taaggaccag
     1741 caactgctgg gactctgggg gtgttccggg aagcttattt gtgccactgc ggtaccatgg
     1801 aactcctctt ggtccaacaa gtctctgggc gatatttggg acaatatgac gtggatgcag
     1861 tgggatcgcg agatatccaa ctacaccaac acgatcttcc ggcttctcga agacagtcag
     1921 aatcaacaag agaagaatga gaaggatctg ttggcccttg acagctggaa aaatctttgg
     1981 aattggtttg acattactaa ctccggaggc agcggacatc atcaccatca ccacggctct
     2041 ggagccggaa gtgctatccc catcatgggt atcgttgctg gcctggttgt ccttgcagct
     2101 gtagtcactg gagctgcggt cgctgctgtg ctgtggagaa agaagagctc agattgactc
     2161 gaggccggca aggccggatc cagacatgat aagatacatt gatgagtttg gacaaaccac
     2221 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     2281 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
     2341 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
     2401 tatggctgat tatgatccgg ctgcctcgcg cgtttcggtg atgacggtga aaacctctga
     2461 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     2521 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gaggtcgact
     2581 ctagaggatc gatccccgcc ccggacgaac taaacctgac tacgacatct ctgccccttc
     2641 ttcgcggggc agtgcatgta atcccttcag ttggttggta caacttgcca actgggccct
     2701 gttccacatg tgacacgggg ggggaccaaa cacaaagggg ttctctgact gtagttgaca
     2761 tccttataaa tggatgtgca catttgccaa cactgagtgg ctttcatcct ggagcagact
     2821 ttgcagtctg tggactgcaa cacaacattg cctttatgtg taactcttgg ctgaagctct
     2881 tacaccaatg ctgggggaca tgtacctccc aggggcccag gaagactacg ggaggctaca
     2941 ccaacgtcaa tcagaggggc ctgtgtagct accgataagc ggaccctcaa gagggcatta
     3001 gcaatagtgt ttataaggcc cccttgttaa ccctaaacgg gtagcatatg cttcccgggt
     3061 agtagtatat actatccaga ctaaccctaa ttcaatagca tatgttaccc aacgggaagc
     3121 atatgctatc gaattagggt tagtaaaagg gtcctaagga acagcgatat ctcccacccc
     3181 atgagctgtc acggttttat ttacatgggg tcaggattcc acgagggtag tgaaccattt
     3241 tagtcacaag ggcagtggct gaagatcaag gagcgggcag tgaactctcc tgaatcttcg
     3301 cctgcttctt cattctcctt cgtttagcta atagaataac tgctgagttg tgaacagtaa
     3361 ggtgtatgtg aggtgctcga aaacaaggtt tcaggtgacg cccccagaat aaaatttgga
     3421 cggggggttc agtggtggca ttgtgctatg acaccaatat aaccctcaca aaccccttgg
     3481 gcaataaata ctagtgtagg aatgaaacat tctgaatatc tttaacaata gaaatccatg
     3541 gggtggggac aagccgtaaa gactggatgt ccatctcaca cgaatttatg gctatgggca
     3601 acacataatc ctagtgcaat atgatactgg ggttattaag atgtgtccca ggcagggacc
     3661 aagacaggtg aaccatgttg ttacactcta tttgtaacaa ggggaaagag agtggacgcc
     3721 gacagcagcg gactccactg gttgtctcta acacccccga aaattaaacg gggctccacg
     3781 ccaatggggc ccataaacaa agacaagtgg ccactctttt ttttgaaatt gtggagtggg
     3841 ggcacgcgtc agcccccaca cgccgccctg cggttttgga ctgtaaaata agggtgtaat
     3901 aacttggctg attgtaaccc cgctaaccac tgcggtcaaa ccacttgccc acaaaaccac
     3961 taatggcacc ccggggaata cctgcataag taggtgggcg ggccaagata ggggcgcgat
     4021 tgctgcgatc tggaggacaa attacacaca cttgcgcctg agcgccaagc acagggttgt
     4081 tggtcctcat attcacgagg tcgctgagag cacggtgggc taatgttgcc atgggtagca
     4141 tatactaccc aaatatctgg atagcatatg ctatcctaat ctatatctgg gtagcatagg
     4201 ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg
     4261 ctatcctaat ttatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg
     4321 ctatcctaat ctatatctgg gtagtatatg ctatcctaat ctgtatccgg gtagcatatg
     4381 ctatcctaat agagattagg gtagtatatg ctatcctaat ttatatctgg gtagcatata
     4441 ctacccaaat atctggatag catatgctat cctaatctat atctgggtag catatgctat
     4501 cctaatctat atctgggtag cataggctat cctaatctat atctgggtag catatgctat
     4561 cctaatctat atctgggtag tatatgctat cctaatttat atctgggtag cataggctat
     4621 cctaatctat atctgggtag catatgctat cctaatctat atctgggtag tatatgctat
     4681 cctaatctgt atccgggtag catatgctat cctcatgcat atacagtcag catatgatac
     4741 ccagtagtag agtgggagtg ctatcctttg catatgccgc cacctcccaa gggggcgtga
     4801 attttcgctg cttgtccttt tcctgctggt tgctcccatt cttaggtgaa tttaaggagg
     4861 ccaggctaaa gccgtcgcat gtctgattgc tcaccaggta aatgtcgcta atgttttcca
     4921 acgcgagaag gtgttgagcg cggagctgag tgacgtgaca acatgggtat gcccaattgc
     4981 cccatgttgg gaggacgaaa atggtgacaa gacagatggc cagaaataca ccaacagcac
     5041 gcatgatgtc tactggggat ttattcttta gtgcggggga atacacggct tttaatacga
     5101 ttgagggcgt ctcctaacaa gttacatcac tcctgccctt cctcaccctc atctccatca
     5161 cctccttcat ctccgtcatc tccgtcatca ccctccgcgg cagccccttc caccataggt
     5221 ggaaaccagg gaggcaaatc tactccatcg tcaaagctgc acacagtcac cctgatattg
     5281 caggtaggag cgggctttgt cataacaagg tccttaatcg catccttcaa aacctcagca
     5341 aatatatgag tttgtaaaaa gaccatgaaa taacagacaa tggactccct tagcgggcca
     5401 ggttgtgggc cgggtccagg ggccattcca aaggggagac gactcaatgg tgtaagacga
     5461 cattgtggaa tagcaagggc agttcctcgc cttaggttgt aaagggaggt cttactacct
     5521 ccatatacga acacaccggc gacccaagtt ccttcgtcgg tagtcctttc tacgtgactc
     5581 ctagccagga gagctcttaa accttctgca atgttctcaa atttcgggtt ggaacctcct
     5641 tgaccacgat gctttccaaa ccaccctcct tttttgcgcc tgcctccatc accctgaccc
     5701 cggggtccag tgcttgggcc ttctcctggg tcatctgcgg ggccctgctc tatcgctccc
     5761 gggggcacgt caggctcacc atctgggcca ccttcttggt ggtattcaaa ataatcggct
     5821 tcccctacag ggtggaaaaa tggccttcta cctggagggg gcctgcgcgg tggagacccg
     5881 gatgatgatg actgactact gggactcctg ggcctctttt ctccacgtcc acgacctctc
     5941 cccctggctc tttcacgact tccccccctg gctctttcac gtcctctacc ccggcggcct
     6001 ccactacctc ctcgaccccg gcctccacta cctcctcgac cccggcctcc actgcctcct
     6061 cgaccccggc ctccacctcc tgctcctgcc cctcctgctc ctgcccctcc tcctgctcct
     6121 gcccctcctg cccctcctgc tcctgcccct cctgcccctc ctgctcctgc ccctcctgcc
     6181 cctcctgctc ctgcccctcc tgcccctcct cctgctcctg cccctcctgc ccctcctcct
     6241 gctcctgccc ctcctgcccc tcctgctcct gcccctcctg cccctcctgc tcctgcccct
     6301 cctgcccctc ctgctcctgc ccctcctgct cctgcccctc ctgctcctgc ccctcctgct
     6361 cctgcccctc ctgcccctcc tgcccctcct cctgctcctg cccctcctgc tcctgcccct
     6421 cctgcccctc ctgcccctcc tgctcctgcc cctcctcctg ctcctgcccc tcctgcccct
     6481 cctgcccctc ctcctgctcc tgcccctcct gcccctcctc ctgctcctgc ccctcctcct
     6541 gctcctgccc ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tgcccctcct
     6601 cctgctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctgcccc tcctcctgct
     6661 cctgcccctc ctcctgctcc tgcccctcct gcccctcctg cccctcctgc ccctcctcct
     6721 gctcctgccc ctcctcctgc tcctgcccct cctgctcctg cccctcccgc tcctgctcct
     6781 gctcctgttc caccgtgggt ccctttgcag ccaatgcaac ttggacgttt ttggggtctc
     6841 cggacaccat ctctatgtct tggccctgat cctgagccgc ccggggctcc tggtcttccg
     6901 cctcctcgtc ctcgtcctct tccccgtcct cgtccatggt tatcaccccc tcttctttga
     6961 ggtccactgc cgccggagcc ttctggtcca gatgtgtctc ccttctctcc taggccattt
     7021 ccaggtcctg tacctggccc ctcgtcagac atgattcaca ctaaaagaga tcaatagaca
     7081 tctttattag acgacgctca gtgaatacag ggagtgcaga ctcctgcccc ctccaacagc
     7141 ccccccaccc tcatcccctt catggtcgct gtcagacaga tccaggtctg aaaattcccc
     7201 atcctccgaa ccatcctcgt cctcatcacc aattactcgc agcccggaaa actcccgctg
     7261 aacatcctca agatttgcgt cctgagcctc aagccaggcc tcaaattcct cgtccccctt
     7321 tttgctggac ggtagggatg gggattctcg ggacccctcc tcttcctctt caaggtcacc
     7381 agacagagat gctactgggg caacggaaga aaagctgggt gcggcctgtg aggatcagct
     7441 tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc
     7501 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     7561 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     7621 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     7681 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     7741 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     7801 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     7861 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     7921 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     7981 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     8041 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     8101 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     8161 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     8221 gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     8281 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     8341 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     8401 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     8461 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     8521 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     8581 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     8641 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     8701 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     8761 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     8821 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     8881 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     8941 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     9001 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     9061 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     9121 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     9181 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     9241 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     9301 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttgaag ctgtccctga
     9361 tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg
     9421 gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag
     9481 acgtagccca gcgcgtcggc cccgagatgc gccgcgtgcg gctgctggag atggcggacg
     9541 cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat
     9601 tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccctgct tcatccccgt
     9661 ggcccgttgc tcgcgtttgc tggcggtgtc cccggaagaa atatatttgc atgtctttag
     9721 ttctatgatg acacaaaccc cgcccagcgt cttgtcattg gcgaattcga acacgcagat
     9781 gcagtcgggg cggcgcggtc cgaggtccac ttcgcatatt aaggtgacgc gtgtggcctc
     9841 gaacaccgag cgaccctgca gcgacccgct taacagcgtc aacagcgtgc cgcagatccc
     9901 ggggggcaat gagatatgaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg
     9961 atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt
    10021 gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat
    10081 ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg
    10141 gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca
    10201 cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc
    10261 gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca
    10321 ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct
    10381 gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg
    10441 caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg
    10501 cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt
    10561 gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg
    10621 aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag
    10681 cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat
    10741 cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca
    10801 atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc
    10861 gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact
    10921 cgtccggatc gggagatggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg
    10981 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacgggtgtt gggtcgtttg
    11041 ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc
    11101 attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg
    11161 aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc actggccccg
    11221 tgggttaggg acggggtccc ccatggggaa tggtttatgg ttcgtggggg ttattatttt
    11281 gggcgttgcg tggggtcagg tccacgactg gactgagcag acagacccat ggtttttgga
    11341 tggcctgggc atggaccgca tgtactggcg cgacacgaac accgggcgtc tgtggctgcc
    11401 aaacaccccc gacccccaaa aaccaccgcg cggatttctg gcgtgccaag ctagtcgacc
    11461 aattctcatg tttgacagct tatcatcgca gatccgggca acgttgttgc cattgctgca
    11521 ggcgcagaac tggtaggtat ggaagatcta tacattgaat caatattggc aattagccat
    11581 attagtcatt ggttatatag cataaatcaa tattggctat tggccattgc atacgttgta
    11641 tctatatcat aatatgtaca tttatattgg ctcatgtcca atatgaccgc catgttgaca
    11701 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
    11761 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
    11821 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
    11881 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
    11941 gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
    12001 ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct acgtattagt
    12061 catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg gatagcggtt
    12121 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
    12181 ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga cgcaaatggg
    12241 cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat
    12301 ctctagaagc tgggtaccag ctgctagc
//
