LOCUS       sequence-235208-       12436 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the 001428 HIV-1 isolate; 
            C-terminal truncation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12436)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 12436)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-001428gp160-754*
FEATURES             Location/Qualifiers
     source          1..12436
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    2914..3048
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2938..2957)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2992..3011
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3190..3212)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      3772..5561
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(5867..7792)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(8227..8245)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8313..8417
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8418..8486,8487..9278)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8636..8655)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9449..10037
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9938..9957
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        10468..10613
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             10656..11693
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(11691..11710)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11735..11783
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatctctag aagctgggta ccagctgcta gcgccaccat gcctatgggg agcctgcaac
      661 cgttggccac gttgtacctc ctgggcatgc tcgtagcaag tgtgctggca gtcgagaatc
      721 tctgggtaac cgtttactat ggggttcccg tgtggaagga ggcgcgcaca actttgtttt
      781 gcgcgtctga tgcgaaggcc tacgagaccg aagtccataa cgtgtgggca acccacgctt
      841 gcgtacctac ggaccctaac ccccaagaga tggtgttggg aaacgtcacg gaaaacttca
      901 acatgtggaa gaatgatatg gtagaccaga tgcacgagga cgtaatttct ttgtgggcac
      961 aaagtctcaa gccttgtgtt aaactgacgc cactttgcgt aaccttggaa tgcacacagg
     1021 tcaacgcgac tcaggggaat acaactcagg taaacgtcac tcaggtcaac ggcgatgaaa
     1081 tgaagaattg cagtttcaac acaacgaccg aaatcagaga caagaaacaa aaagcgtatg
     1141 cactgttcta tcgactggac ctcgttccgt tggagagaga gaacagagga gattcaaatt
     1201 ccgcatctaa gtacattctc atcaattgta acacctctgc catcacgcag gcgtgcccaa
     1261 aagtaaattt cgaccctatc ccaatacatt actgcacccc ggcaggctat gcaatactca
     1321 aatgcaataa taagacattt aacggcacag gttcttgcaa caacgttagt acagtgcaat
     1381 gcactcacgg gattaagccc gttgttagca cacaactgct gcttaatggt tcattggccg
     1441 aagaggagat tatcattaga agcgaaaacc tcaccgataa tgtaaagacc atcattgtcc
     1501 acctggatca gagcgtggag atcgtgtgta ctagaccgaa taacaacacc cggaagagta
     1561 tcagaatcgg gccaggccag acattctatg ctactggaga cataatcgga aacattcgag
     1621 aggcccactg caacatctcc gaaaaaaagt ggcatgaaat gctgaggaga gtgtctgaaa
     1681 agcttgcgga gcactttcca aacaagacca tcaaatttac ttctagcagt ggtggcgact
     1741 tggagataac gactcatagt tttaactgca gaggagaatt tttttactgc aatacgtctg
     1801 ggcttttcaa ctccacatat atgccgaacg gaacatatat gcccaatggc acgaataaca
     1861 gcaattccac gataattctg ccctgtagaa taaagcaaat tataaatatg tggcaagagg
     1921 tagggagagc gatgtatgct ccaccaatag ctggaaacat tacttgtaat tctaatataa
     1981 ccgggctctt gctcgtgagg gacggaggaa aaaataacaa cactgagata ttccgccccg
     2041 gcggggggga tatgcgggac aactggagaa gcgaacttta taagtacaag gtcgttgaga
     2101 ttaaacccct gggcgtcgcc ccgacgaggg cgaagcgacg ggttgttgag agggaaaaac
     2161 gagcggtcgg cctcggcgcc gttttcctcg gtttcttggg ggcagctgga tctactatgg
     2221 gagccgcatc aatcactttg acagtgcagg ccagacaatt gctgtcagga atcgttcagc
     2281 agcaatcaaa tctcttgcaa gctatcgagg ctcaacaaca cttgttgcag cttacggttt
     2341 gggggatcaa acagcttcaa acccgggtac ttgctatcga aagatatttg aaggaccaac
     2401 aactcctcgg tatctggggg tgcagtggaa aattgatctg tactactgcc gtgccttgga
     2461 attctagttg gtctaacaaa agtctcacgg acatctggga caatatgaca tggatgcagt
     2521 gggatagaga ggtgagcaac tataccggaa ttatttatcg ccttctggag gactctcaga
     2581 atcaacaaga gaggaacgag caagacctcc tcgcgctcga ttcctggaag aatctttggt
     2641 catggtttga tatctcaaat tggctctggt atataaaaat tttcataatg atagtaggtg
     2701 ggttgattgg tcttcgcatc atttttgcgg tattgtctat cgtcaaccga gtaagacagg
     2761 ggtattcccc attgtctttc caaacattga ccccaaaccc gactggcccc gacagactcg
     2821 ggagaatcga agaagaggga ggtgagcagg atagagatag gagcgtgagg cttgtgaacg
     2881 gtttcctgtg ataactcgag gccggcaagg ccggatccag acatgataag atacattgat
     2941 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     3001 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     3061 tgcattcatt ttatgtttca ggttcagggg gaggtgggga ggttttttaa agcaagtaaa
     3121 acctctacaa atgtggtatg gctgattatg atccggctgc ctcgcgcgtt tcggtgatga
     3181 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     3241 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc
     3301 agccatgagg tcgactctag aggatcgatc cccgccccgg acgaactaaa cctgactacg
     3361 acatctctgc cccttcttcg cggggcagtg catgtaatcc cttcagttgg ttggtacaac
     3421 ttgccaactg ggccctgttc cacatgtgac acgggggggg accaaacaca aaggggttct
     3481 ctgactgtag ttgacatcct tataaatgga tgtgcacatt tgccaacact gagtggcttt
     3541 catcctggag cagactttgc agtctgtgga ctgcaacaca acattgcctt tatgtgtaac
     3601 tcttggctga agctcttaca ccaatgctgg gggacatgta cctcccaggg gcccaggaag
     3661 actacgggag gctacaccaa cgtcaatcag aggggcctgt gtagctaccg ataagcggac
     3721 cctcaagagg gcattagcaa tagtgtttat aaggccccct tgttaaccct aaacgggtag
     3781 catatgcttc ccgggtagta gtatatacta tccagactaa ccctaattca atagcatatg
     3841 ttacccaacg ggaagcatat gctatcgaat tagggttagt aaaagggtcc taaggaacag
     3901 cgatatctcc caccccatga gctgtcacgg ttttatttac atggggtcag gattccacga
     3961 gggtagtgaa ccattttagt cacaagggca gtggctgaag atcaaggagc gggcagtgaa
     4021 ctctcctgaa tcttcgcctg cttcttcatt ctccttcgtt tagctaatag aataactgct
     4081 gagttgtgaa cagtaaggtg tatgtgaggt gctcgaaaac aaggtttcag gtgacgcccc
     4141 cagaataaaa tttggacggg gggttcagtg gtggcattgt gctatgacac caatataacc
     4201 ctcacaaacc ccttgggcaa taaatactag tgtaggaatg aaacattctg aatatcttta
     4261 acaatagaaa tccatggggt ggggacaagc cgtaaagact ggatgtccat ctcacacgaa
     4321 tttatggcta tgggcaacac ataatcctag tgcaatatga tactggggtt attaagatgt
     4381 gtcccaggca gggaccaaga caggtgaacc atgttgttac actctatttg taacaagggg
     4441 aaagagagtg gacgccgaca gcagcggact ccactggttg tctctaacac ccccgaaaat
     4501 taaacggggc tccacgccaa tggggcccat aaacaaagac aagtggccac tctttttttt
     4561 gaaattgtgg agtgggggca cgcgtcagcc cccacacgcc gccctgcggt tttggactgt
     4621 aaaataaggg tgtaataact tggctgattg taaccccgct aaccactgcg gtcaaaccac
     4681 ttgcccacaa aaccactaat ggcaccccgg ggaatacctg cataagtagg tgggcgggcc
     4741 aagatagggg cgcgattgct gcgatctgga ggacaaatta cacacacttg cgcctgagcg
     4801 ccaagcacag ggttgttggt cctcatattc acgaggtcgc tgagagcacg gtgggctaat
     4861 gttgccatgg gtagcatata ctacccaaat atctggatag catatgctat cctaatctat
     4921 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
     4981 atctgggtag tatatgctat cctaatttat atctgggtag cataggctat cctaatctat
     5041 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatctgt
     5101 atccgggtag catatgctat cctaatagag attagggtag tatatgctat cctaatttat
     5161 atctgggtag catatactac ccaaatatct ggatagcata tgctatccta atctatatct
     5221 gggtagcata tgctatccta atctatatct gggtagcata ggctatccta atctatatct
     5281 gggtagcata tgctatccta atctatatct gggtagtata tgctatccta atttatatct
     5341 gggtagcata ggctatccta atctatatct gggtagcata tgctatccta atctatatct
     5401 gggtagtata tgctatccta atctgtatcc gggtagcata tgctatcctc atgcatatac
     5461 agtcagcata tgatacccag tagtagagtg ggagtgctat cctttgcata tgccgccacc
     5521 tcccaagggg gcgtgaattt tcgctgcttg tccttttcct gcatgctggt tgctcccatt
     5581 cttaggtgaa tttaaggagg ccaggctaaa gccgtcgcat gtctgattgc tcaccaggta
     5641 aatgtcgcta atgttttcca acgcgagaag gtgttgagcg cggagctgag tgacgtgaca
     5701 acatgggtat gcccaattgc cccatgttgg gaggacgaaa atggtgacaa gacagatggc
     5761 cagaaataca ccaacagcac gcatgatgtc tactggggat ttattcttta gtgcggggga
     5821 atacacggct tttaatacga ttgagggcgt ctcctaacaa gttacatcac tcctgccctt
     5881 cctcaccctc atctccatca cctccttcat ctccgtcatc tccgtcatca ccctccgcgg
     5941 cagccccttc caccataggt ggaaaccagg gaggcaaatc tactccatcg tcaaagctgc
     6001 acacagtcac cctgatattg caggtaggag cgggctttgt cataacaagg tccttaatcg
     6061 catccttcaa aacctcagca aatatatgag tttgtaaaaa gaccatgaaa taacagacaa
     6121 tggactccct tagcgggcca ggttgtgggc cgggtccagg ggccattcca aaggggagac
     6181 gactcaatgg tgtaagacga cattgtggaa tagcaagggc agttcctcgc cttaggttgt
     6241 aaagggaggt cttactacct ccatatacga acacaccggc gacccaagtt ccttcgtcgg
     6301 tagtcctttc tacgtgactc ctagccagga gagctcttaa accttctgca atgttctcaa
     6361 atttcgggtt ggaacctcct tgaccacgat gctttccaaa ccaccctcct tttttgcgcc
     6421 tgcctccatc accctgaccc cggggtccag tgcttgggcc ttctcctggg tcatctgcgg
     6481 ggccctgctc tatcgctccc gggggcacgt caggctcacc atctgggcca ccttcttggt
     6541 ggtattcaaa ataatcggct tcccctacag ggtggaaaaa tggccttcta cctggagggg
     6601 gcctgcgcgg tggagacccg gatgatgatg actgactact gggactcctg ggcctctttt
     6661 ctccacgtcc acgacctctc cccctggctc tttcacgact tccccccctg gctctttcac
     6721 gtcctctacc ccggcggcct ccactacctc ctcgaccccg gcctccacta cctcctcgac
     6781 cccggcctcc actgcctcct cgaccccggc ctccacctcc tgctcctgcc cctcctgctc
     6841 ctgcccctcc tcctgctcct gcccctcctg cccctcctgc tcctgcccct cctgcccctc
     6901 ctgctcctgc ccctcctgcc cctcctgctc ctgcccctcc tgcccctcct cctgctcctg
     6961 cccctcctgc ccctcctcct gctcctgccc ctcctgcccc tcctgctcct gcccctcctg
     7021 cccctcctgc tcctgcccct cctgcccctc ctgctcctgc ccctcctgct cctgcccctc
     7081 ctgctcctgc ccctcctgct cctgcccctc ctgcccctcc tgcccctcct cctgctcctg
     7141 cccctcctgc tcctgcccct cctgcccctc ctgcccctcc tgctcctgcc cctcctcctg
     7201 ctcctgcccc tcctgcccct cctgcccctc ctcctgctcc tgcccctcct gcccctcctc
     7261 ctgctcctgc ccctcctcct gctcctgccc ctcctgcccc tcctgcccct cctcctgctc
     7321 ctgcccctcc tgcccctcct cctgctcctg cccctcctcc tgctcctgcc cctcctgccc
     7381 ctcctgcccc tcctcctgct cctgcccctc ctcctgctcc tgcccctcct gcccctcctg
     7441 cccctcctgc ccctcctcct gctcctgccc ctcctcctgc tcctgcccct cctgctcctg
     7501 cccctcccgc tcctgctcct gctcctgttc caccgtgggt ccctttgcag ccaatgcaac
     7561 ttggacgttt ttggggtctc cggacaccat ctctatgtct tggccctgat cctgagccgc
     7621 ccggggctcc tggtcttccg cctcctcgtc ctcgtcctct tccccgtcct cgtccatggt
     7681 tatcaccccc tcttctttga ggtccactgc cgccggagcc ttctggtcca gatgtgtctc
     7741 ccttctctcc taggccattt ccaggtcctg tacctggccc ctcgtcagac atgattcaca
     7801 ctaaaagaga tcaatagaca tctttattag acgacgctca gtgaatacag ggagtgcaga
     7861 ctcctgcccc ctccaacagc ccccccaccc tcatcccctt catggtcgct gtcagacaga
     7921 tccaggtctg aaaattcccc atcctccgaa ccatcctcgt cctcatcacc aattactcgc
     7981 agcccggaaa actcccgctg aacatcctca agatttgcgt cctgagcctc aagccaggcc
     8041 tcaaattcct cgtccccctt tttgctggac ggtagggatg gggattctcg ggacccctcc
     8101 tcttcctctt caaggtcacc agacagagat gctactgggg caacggaaga aaagctgggt
     8161 gcggcctgtg aggatcagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac
     8221 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     8281 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     8341 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     8401 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     8461 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     8521 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     8581 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     8641 gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact
     8701 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     8761 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     8821 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     8881 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     8941 agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     9001 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     9061 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     9121 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     9181 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     9241 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     9301 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     9361 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     9421 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     9481 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     9541 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     9601 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     9661 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     9721 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     9781 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     9841 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     9901 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     9961 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
    10021 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
    10081 ggccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg
    10141 cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc
    10201 gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cccgagatgc gccgcgtgcg
    10261 gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac
    10321 agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg
    10381 ccgccctgct tcatccccgt ggcccgttgc tcgcgtttgc tggcggtgtc cccggaagaa
    10441 atatatttgc atgtctttag ttctatgatg acacaaaccc cgcccagcgt cttgtcattg
    10501 gcgaattcga acacgcagat gcagtcgggg cggcgcggtc cgaggtccac ttcgcatatt
    10561 aaggtgacgc gtgtggcctc gaacaccgag cgaccctgca gcgacccgct taacagcgtc
    10621 aacagcgtgc cgcagatccc ggggggcaat gagatatgaa aaagcctgaa ctcaccgcga
    10681 cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct
    10741 cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc
    10801 gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat
    10861 cggccgcgct cccgattccg gaagtgcttg acattgggga attcagcgag agcctgacct
    10921 attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc
    10981 ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc
    11041 agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg
    11101 atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca
    11161 ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc
    11221 ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg
    11281 gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg
    11341 tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact
    11401 tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca
    11461 ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg
    11521 cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa
    11581 tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg
    11641 gaaaccgacg ccccagcact cgtccggatc gggagatggg ggaggctaac tgaaacacgg
    11701 aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg
    11761 cacgggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt
    11821 cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc
    11881 ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc
    11941 tgccatagcc actggccccg tgggttaggg acggggtccc ccatggggaa tggtttatgg
    12001 ttcgtggggg ttattatttt gggcgttgcg tggggtcagg tccacgactg gactgagcag
    12061 acagacccat ggtttttgga tggcctgggc atggaccgca tgtactggcg cgacacgaac
    12121 accgggcgtc tgtggctgcc aaacaccccc gacccccaaa aaccaccgcg cggatttctg
    12181 gcgtgccaag ctagtcgacc aattctcatg tttgacagct tatcatcgca gatccgggca
    12241 acgttgttgc cattgctgca ggcgcagaac tggtaggtat ggaagatcta tacattgaat
    12301 caatattggc aattagccat attagtcatt ggttatatag cataaatcaa tattggctat
    12361 tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg ctcatgtcca
    12421 atatgaccgc catgtt
//
