LOCUS       sequence-235211-       12437 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the 001428 HIV-1 isolate; 
            C-terminal truncation; QES mutant with enhanced binding to PG16.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12437)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 12437)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-001428-QES.c10.L544Y-754*
FEATURES             Location/Qualifiers
     source          1..12437
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    2282..2416
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2306..2325)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2360..2379
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2559..2581)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      3141..4930
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(5236..7161)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(7596..7614)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7682..7786
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7787..7855,7856..8647)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8005..8024)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8818..9406
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9307..9326
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        9837..9982
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             10025..11062
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(11060..11079)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11104..11152
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        11806..12185
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12186..12389
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     12340..12360
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     12386..12410
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gccaccatgc ctatggggag cctgcaaccg ttggccacgt tgtacctcct gggcatgctc
       61 gtagcaagtg tgctggcagt cgagaatctc tgggtaaccg tttactatgg ggttcccgtg
      121 tggaaggagg cgcgcacaac tttgttttgc gcgtctgatg cgaaggccta cgagaccgaa
      181 gtccataacg tgtgggcaac ccacgcttgc gtacctacgg accctaaccc ccaagagatg
      241 gtgttgggaa acgtcacgga aaacttcaac atgtggaaga atgatatggt agaccagatg
      301 cacgaggacg taatttcttt gtgggcacaa agtctcaagc cttgtgttaa actgacgcca
      361 ctttgcgtaa ccttggaatg cacacaggtc aacgcgactc aggggaatac aactcaggta
      421 aacgtcactc aggtcaacgg cgatgaaatg aagaattgca gtttcaacac aacgaccgaa
      481 atcagagaca agaaacaaaa agcgtatgca ctgttctatc gactggacct cgttccgttg
      541 gagagagaga acagaggaga ttcaaattcc gcatctaagt acattctcat caattgtaac
      601 acctctgcca tcacgcaggc gtgcccaaaa atgaatttcg accctatccc aatacattac
      661 tgcaccccgg caggctatgc aatactcaaa tgcaataata agacatttaa cggcacaggt
      721 tcttgcaaca acgttagtac agtgcaatgc actcacggga ttaagcccac tatgagcaca
      781 caactgctgc ttaatggttc attggccgaa gaggagatta tcattagaag cgaaaacctc
      841 accgataatg taaagaccat cattgtccac ctggatcaga gcgtggagat cgtgtgtact
      901 agaccgaata acaacacccg gaagagtatc agaatcgggc caggccagac attctatgct
      961 actggagaca taatcggaaa cattcgagag gcccactgca acatctccga aaaaaagtgg
     1021 catgaaatgc tgaggagagt gtctgaaaag cttgcggagc actttccaaa caagaccatc
     1081 aaatttactt ctagcagtgg tggcgacttg gagataacga ctcatagttt taactgcaga
     1141 ggagaatttt tttactgcaa tacgtctggg cttttcaact ccacatatat gccgaacgga
     1201 acatatatgc ccaatggcac gaataacagc aattccacga taattctgcc ctgtagaata
     1261 aagcaaatta taaatatgtg gcaagaggta gggagagcga tgtatgctcc accaatagct
     1321 ggaaacatta cttgtaattc taatataacc gggctcttgc tcgtgaggga cggaggaaaa
     1381 aataacaaca ctgagatatt ccgccccggc gggggggata tgcgggacaa ctggagaagc
     1441 gaactttata agtacaaggt cgttgagatt aaacccctgg gcgtcgcccc gacgagggcg
     1501 aagcgacggg ttgttgagag ggaaaaacga gcggtcggcc tcggcgccgt tttcctcggt
     1561 ttcttggggg cagctggatc tactatggga gccgcatcaa tcactttgac agtgcaggcc
     1621 agacaatacc tgtcaggaat cgttcagcag caatcaaatc tcttgcaagc tatcgaggct
     1681 caacaacact tgttgcagct tacggtttgg gggatcaaac agcttcaaac ccgggtactt
     1741 gctatcgaaa gatatttgaa ggaccaacaa ctcctcggta tctgggggtg cagtggaaaa
     1801 ttgatctgta ctactgccgt gccttggaat tctagttggt ctaacaaaag tctcacggac
     1861 atctgggaca atatgacatg gatgcagtgg gatagagagg tgagcaacta taccggaatt
     1921 atttatcgcc ttctggagga ctctcagaat caacaagaga ggaacgagca agacctcctc
     1981 gcgctcgatt cctggaagaa tctttggtca tggtttgata tctcaaattg gctctggtat
     2041 ataaaaattt tcataatgat agtaggtggg ttgattggtc ttcgcatcat ttttgcggta
     2101 ttgtctatcg tcaaccgagt aagacagggg tattccccat tgtctttcca aacattgacc
     2161 ccaaacccga ctggccccga cagactcggg agaatcgaag aagagggagg tgagcaggat
     2221 agagatagga gcgtgaggct tgtgaacggt ttcctgtgat aactcgaggc cggcaaggcc
     2281 ggatccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     2341 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     2401 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     2461 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga
     2521 tccggctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     2581 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     2641 tcagcgggtg ttggcgggtg tcggggcgca gccatgaggt cgactctaga ggatcgatcc
     2701 ccgccccgga cgaactaaac ctgactacga catctctgcc ccttcttcgc ggggcagtgc
     2761 atgtaatccc ttcagttggt tggtacaact tgccaactgg gccctgttcc acatgtgaca
     2821 cgggggggga ccaaacacaa aggggttctc tgactgtagt tgacatcctt ataaatggat
     2881 gtgcacattt gccaacactg agtggctttc atcctggagc agactttgca gtctgtggac
     2941 tgcaacacaa cattgccttt atgtgtaact cttggctgaa gctcttacac caatgctggg
     3001 ggacatgtac ctcccagggg cccaggaaga ctacgggagg ctacaccaac gtcaatcaga
     3061 ggggcctgtg tagctaccga taagcggacc ctcaagaggg cattagcaat agtgtttata
     3121 aggccccctt gttaacccta aacgggtagc atatgcttcc cgggtagtag tatatactat
     3181 ccagactaac cctaattcaa tagcatatgt tacccaacgg gaagcatatg ctatcgaatt
     3241 agggttagta aaagggtcct aaggaacagc gatatctccc accccatgag ctgtcacggt
     3301 tttatttaca tggggtcagg attccacgag ggtagtgaac cattttagtc acaagggcag
     3361 tggctgaaga tcaaggagcg ggcagtgaac tctcctgaat cttcgcctgc ttcttcattc
     3421 tccttcgttt agctaataga ataactgctg agttgtgaac agtaaggtgt atgtgaggtg
     3481 ctcgaaaaca aggtttcagg tgacgccccc agaataaaat ttggacgggg ggttcagtgg
     3541 tggcattgtg ctatgacacc aatataaccc tcacaaaccc cttgggcaat aaatactagt
     3601 gtaggaatga aacattctga atatctttaa caatagaaat ccatggggtg gggacaagcc
     3661 gtaaagactg gatgtccatc tcacacgaat ttatggctat gggcaacaca taatcctagt
     3721 gcaatatgat actggggtta ttaagatgtg tcccaggcag ggaccaagac aggtgaacca
     3781 tgttgttaca ctctatttgt aacaagggga aagagagtgg acgccgacag cagcggactc
     3841 cactggttgt ctctaacacc cccgaaaatt aaacggggct ccacgccaat ggggcccata
     3901 aacaaagaca agtggccact cttttttttg aaattgtgga gtgggggcac gcgtcagccc
     3961 ccacacgccg ccctgcggtt ttggactgta aaataagggt gtaataactt ggctgattgt
     4021 aaccccgcta accactgcgg tcaaaccact tgcccacaaa accactaatg gcaccccggg
     4081 gaatacctgc ataagtaggt gggcgggcca agataggggc gcgattgctg cgatctggag
     4141 gacaaattac acacacttgc gcctgagcgc caagcacagg gttgttggtc ctcatattca
     4201 cgaggtcgct gagagcacgg tgggctaatg ttgccatggg tagcatatac tacccaaata
     4261 tctggatagc atatgctatc ctaatctata tctgggtagc ataggctatc ctaatctata
     4321 tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc ctaatttata
     4381 tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata
     4441 tctgggtagt atatgctatc ctaatctgta tccgggtagc atatgctatc ctaatagaga
     4501 ttagggtagt atatgctatc ctaatttata tctgggtagc atatactacc caaatatctg
     4561 gatagcatat gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     4621 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     4681 ggtagtatat gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg
     4741 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg
     4801 ggtagcatat gctatcctca tgcatataca gtcagcatat gatacccagt agtagagtgg
     4861 gagtgctatc ctttgcatat gccgccacct cccaaggggg cgtgaatttt cgctgcttgt
     4921 ccttttcctg catgctggtt gctcccattc ttaggtgaat ttaaggaggc caggctaaag
     4981 ccgtcgcatg tctgattgct caccaggtaa atgtcgctaa tgttttccaa cgcgagaagg
     5041 tgttgagcgc ggagctgagt gacgtgacaa catgggtatg cccaattgcc ccatgttggg
     5101 aggacgaaaa tggtgacaag acagatggcc agaaatacac caacagcacg catgatgtct
     5161 actggggatt tattctttag tgcgggggaa tacacggctt ttaatacgat tgagggcgtc
     5221 tcctaacaag ttacatcact cctgcccttc ctcaccctca tctccatcac ctccttcatc
     5281 tccgtcatct ccgtcatcac cctccgcggc agccccttcc accataggtg gaaaccaggg
     5341 aggcaaatct actccatcgt caaagctgca cacagtcacc ctgatattgc aggtaggagc
     5401 gggctttgtc ataacaaggt ccttaatcgc atccttcaaa acctcagcaa atatatgagt
     5461 ttgtaaaaag accatgaaat aacagacaat ggactccctt agcgggccag gttgtgggcc
     5521 gggtccaggg gccattccaa aggggagacg actcaatggt gtaagacgac attgtggaat
     5581 agcaagggca gttcctcgcc ttaggttgta aagggaggtc ttactacctc catatacgaa
     5641 cacaccggcg acccaagttc cttcgtcggt agtcctttct acgtgactcc tagccaggag
     5701 agctcttaaa ccttctgcaa tgttctcaaa tttcgggttg gaacctcctt gaccacgatg
     5761 ctttccaaac caccctcctt ttttgcgcct gcctccatca ccctgacccc ggggtccagt
     5821 gcttgggcct tctcctgggt catctgcggg gccctgctct atcgctcccg ggggcacgtc
     5881 aggctcacca tctgggccac cttcttggtg gtattcaaaa taatcggctt cccctacagg
     5941 gtggaaaaat ggccttctac ctggaggggg cctgcgcggt ggagacccgg atgatgatga
     6001 ctgactactg ggactcctgg gcctcttttc tccacgtcca cgacctctcc ccctggctct
     6061 ttcacgactt ccccccctgg ctctttcacg tcctctaccc cggcggcctc cactacctcc
     6121 tcgaccccgg cctccactac ctcctcgacc ccggcctcca ctgcctcctc gaccccggcc
     6181 tccacctcct gctcctgccc ctcctgctcc tgcccctcct cctgctcctg cccctcctgc
     6241 ccctcctgct cctgcccctc ctgcccctcc tgctcctgcc cctcctgccc ctcctgctcc
     6301 tgcccctcct gcccctcctc ctgctcctgc ccctcctgcc cctcctcctg ctcctgcccc
     6361 tcctgcccct cctgctcctg cccctcctgc ccctcctgct cctgcccctc ctgcccctcc
     6421 tgctcctgcc cctcctgctc ctgcccctcc tgctcctgcc cctcctgctc ctgcccctcc
     6481 tgcccctcct gcccctcctc ctgctcctgc ccctcctgct cctgcccctc ctgcccctcc
     6541 tgcccctcct gctcctgccc ctcctcctgc tcctgcccct cctgcccctc ctgcccctcc
     6601 tcctgctcct gcccctcctg cccctcctcc tgctcctgcc cctcctcctg ctcctgcccc
     6661 tcctgcccct cctgcccctc ctcctgctcc tgcccctcct gcccctcctc ctgctcctgc
     6721 ccctcctcct gctcctgccc ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc
     6781 tcctgctcct gcccctcctg cccctcctgc ccctcctgcc cctcctcctg ctcctgcccc
     6841 tcctcctgct cctgcccctc ctgctcctgc ccctcccgct cctgctcctg ctcctgttcc
     6901 accgtgggtc cctttgcagc caatgcaact tggacgtttt tggggtctcc ggacaccatc
     6961 tctatgtctt ggccctgatc ctgagccgcc cggggctcct ggtcttccgc ctcctcgtcc
     7021 tcgtcctctt ccccgtcctc gtccatggtt atcaccccct cttctttgag gtccactgcc
     7081 gccggagcct tctggtccag atgtgtctcc cttctctcct aggccatttc caggtcctgt
     7141 acctggcccc tcgtcagaca tgattcacac taaaagagat caatagacat ctttattaga
     7201 cgacgctcag tgaatacagg gagtgcagac tcctgccccc tccaacagcc cccccaccct
     7261 catccccttc atggtcgctg tcagacagat ccaggtctga aaattcccca tcctccgaac
     7321 catcctcgtc ctcatcacca attactcgca gcccggaaaa ctcccgctga acatcctcaa
     7381 gatttgcgtc ctgagcctca agccaggcct caaattcctc gtcccccttt ttgctggacg
     7441 gtagggatgg ggattctcgg gacccctcct cttcctcttc aaggtcacca gacagagatg
     7501 ctactggggc aacggaagaa aagctgggtg cggcctgtga ggatcagctt atcgatgata
     7561 agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata
     7621 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     7681 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     7741 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     7801 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     7861 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     7921 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     7981 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg
     8041 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     8101 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     8161 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     8221 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     8281 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc
     8341 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     8401 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     8461 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     8521 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     8581 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     8641 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     8701 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     8761 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     8821 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     8881 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     8941 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     9001 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     9061 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     9121 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     9181 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     9241 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     9301 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     9361 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     9421 ggccttttta cggttcctgg ccttttgctg gccttgaagc tgtccctgat ggtcgtcatc
     9481 tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag
     9541 aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag
     9601 cgcgtcggcc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg
     9661 ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt
     9721 cttggagtgg tgaatccgtt agcgaggtgc cgccctgctt catccccgtg gcccgttgct
     9781 cgcgtttgct ggcggtgtcc ccggaagaaa tatatttgca tgtctttagt tctatgatga
     9841 cacaaacccc gcccagcgtc ttgtcattgg cgaattcgaa cacgcagatg cagtcggggc
     9901 ggcgcggtcc gaggtccact tcgcatatta aggtgacgcg tgtggcctcg aacaccgagc
     9961 gaccctgcag cgacccgctt aacagcgtca acagcgtgcc gcagatcccg gggggcaatg
    10021 agatatgaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt
    10081 cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt
    10141 cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa
    10201 agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga
    10261 cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac
    10321 gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat
    10381 ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca
    10441 aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt
    10501 gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga
    10561 tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt
    10621 cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga
    10681 ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt
    10741 ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc
    10801 gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt
    10861 tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc
    10921 cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga
    10981 tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccggatcg
    11041 ggagatgggg gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta
    11101 tgacggcaat aaaaagacag aataaaacgc acgggtgttg ggtcgtttgt tcataaacgc
    11161 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa
    11221 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg
    11281 ctcgcagcca acgtcggggc ggcaggccct gccatagcca ctggccccgt gggttaggga
    11341 cggggtcccc catggggaat ggtttatggt tcgtgggggt tattattttg ggcgttgcgt
    11401 ggggtcaggt ccacgactgg actgagcaga cagacccatg gtttttggat ggcctgggca
    11461 tggaccgcat gtactggcgc gacacgaaca ccgggcgtct gtggctgcca aacacccccg
    11521 acccccaaaa accaccgcgc ggatttctgg cgtgccaagc tagtcgacca attctcatgt
    11581 ttgacagctt atcatcgcag atccgggcaa cgttgttgcc attgctgcag gcgcagaact
    11641 ggtaggtatg gaagatctat acattgaatc aatattggca attagccata ttagtcattg
    11701 gttatatagc ataaatcaat attggctatt ggccattgca tacgttgtat ctatatcata
    11761 atatgtacat ttatattggc tcatgtccaa tatgaccgcc atgttgacat tgattattga
    11821 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
    11881 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
    11941 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
    12001 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
    12061 caagtccgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
    12121 acatgacctt acgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
    12181 ccatggtgat gcggttttgg cagtacacca atgggcgtgg atagcggttt gactcacggg
    12241 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
    12301 gggactttcc aaaatgtcgt aataaccccg ccccgttgac gcaaatgggc ggtaggcgtg
    12361 tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc tctagaagct
    12421 gggtaccagc tgctagc
//
