LOCUS       sequence-235217-       12694 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the 001428 HIV-1 isolate; 
            C-terminal truncation fused to the C-terminal half of split 
            fluorescent Venus (VC).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12694)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 12694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-001428(753)-VC
FEATURES             Location/Qualifiers
     source          1..12694
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2269..2520
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     2457..2478
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    2543..2677
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2567..2586)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2621..2640
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2820..2842)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      3402..5191
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(5493..7418)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(7853..7871)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7939..8043
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8044..8112,8113..8904)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8262..8281)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9075..9663
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9564..9583
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        10094..10239
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             10282..11319
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(11317..11336)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11361..11409
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        12063..12442
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12443..12646
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     12597..12617
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     12643..12667
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gccaccatgc ctatggggag cctgcaaccg ttggccacgt tgtacctcct gggcatgctc
       61 gtagcaagtg tgctggcagt cgagaatctc tgggtaaccg tttactatgg ggttcccgtg
      121 tggaaggagg cgcgcacaac tttgttttgc gcgtctgatg cgaaggccta cgagaccgaa
      181 gtccataacg tgtgggcaac ccacgcttgc gtacctacgg accctaaccc ccaagagatg
      241 gtgttgggaa acgtcacgga aaacttcaac atgtggaaga atgatatggt agaccagatg
      301 cacgaggacg taatttcttt gtgggcacaa agtctcaagc cttgtgttaa actgacgcca
      361 ctttgcgtaa ccttggaatg cacacaggtc aacgcgactc aggggaatac aactcaggta
      421 aacgtcactc aggtcaacgg cgatgaaatg aagaattgca gtttcaacac aacgaccgaa
      481 atcagagaca agaaacaaaa agcgtatgca ctgttctatc gactggacct cgttccgttg
      541 gagagagaga acagaggaga ttcaaattcc gcatctaagt acattctcat caattgtaac
      601 acctctgcca tcacgcaggc gtgcccaaaa gtaaatttcg accctatccc aatacattac
      661 tgcaccccgg caggctatgc aatactcaaa tgcaataata agacatttaa cggcacaggt
      721 tcttgcaaca acgttagtac agtgcaatgc actcacggga ttaagcccgt tgttagcaca
      781 caactgctgc ttaatggttc attggccgaa gaggagatta tcattagaag cgaaaacctc
      841 accgataatg taaagaccat cattgtccac ctggatcaga gcgtggagat cgtgtgtact
      901 agaccgaata acaacacccg gaagagtatc agaatcgggc caggccagac attctatgct
      961 actggagaca taatcggaaa cattcgagag gcccactgca acatctccga aaaaaagtgg
     1021 catgaaatgc tgaggagagt gtctgaaaag cttgcggagc actttccaaa caagaccatc
     1081 aaatttactt ctagcagtgg tggcgacttg gagataacga ctcatagttt taactgcaga
     1141 ggagaatttt tttactgcaa tacgtctggg cttttcaact ccacatatat gccgaacgga
     1201 acatatatgc ccaatggcac gaataacagc aattccacga taattctgcc ctgtagaata
     1261 aagcaaatta taaatatgtg gcaagaggta gggagagcga tgtatgctcc accaatagct
     1321 ggaaacatta cttgtaattc taatataacc gggctcttgc tcgtgaggga cggaggaaaa
     1381 aataacaaca ctgagatatt ccgccccggc gggggggata tgcgggacaa ctggagaagc
     1441 gaactttata agtacaaggt cgttgagatt aaacccctgg gcgtcgcccc gacgagggcg
     1501 aagcgacggg ttgttgagag ggaaaaacga gcggtcggcc tcggcgccgt tttcctcggt
     1561 ttcttggggg cagctggatc tactatggga gccgcatcaa tcactttgac agtgcaggcc
     1621 agacaattgc tgtcaggaat cgttcagcag caatcaaatc tcttgcaagc tatcgaggct
     1681 caacaacact tgttgcagct tacggtttgg gggatcaaac agcttcaaac ccgggtactt
     1741 gctatcgaaa gatatttgaa ggaccaacaa ctcctcggta tctgggggtg cagtggaaaa
     1801 ttgatctgta ctactgccgt gccttggaat tctagttggt ctaacaaaag tctcacggac
     1861 atctgggaca atatgacatg gatgcagtgg gatagagagg tgagcaacta taccggaatt
     1921 atttatcgcc ttctggagga ctctcagaat caacaagaga ggaacgagca agacctcctc
     1981 gcgctcgatt cctggaagaa tctttggtca tggtttgata tctcaaattg gctctggtat
     2041 ataaaaattt tcataatgat agtaggtggg ttgattggtc ttcgcatcat ttttgcggta
     2101 ttgtctatcg tcaaccgagt aagacagggg tattccccat tgtctttcca aacattgacc
     2161 ccaaacccga ctggccccga cagactcggg agaatcgaag aagagggagg tgagcaggat
     2221 agagatagga gcgtgaggct tgtgaacggt ttcctgggag gctctggaga caagcagaag
     2281 aacggcatca aggccaactt caagatccgc cacaacatcg aggacggcgg cgtgcagctc
     2341 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     2401 cactacctga gctaccagtc caagctgagc aaagacccca acgagaagcg cgatcacatg
     2461 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     2521 taactcgagg ccggcaaggc cggatccaga catgataaga tacattgatg agtttggaca
     2581 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     2641 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     2701 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa
     2761 atgtggtatg gctgattatg atccggctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     2821 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     2881 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgagg
     2941 tcgactctag aggatcgatc cccgccccgg acgaactaaa cctgactacg acatctctgc
     3001 cccttcttcg cggggcagtg catgtaatcc cttcagttgg ttggtacaac ttgccaactg
     3061 ggccctgttc cacatgtgac acgggggggg accaaacaca aaggggttct ctgactgtag
     3121 ttgacatcct tataaatgga tgtgcacatt tgccaacact gagtggcttt catcctggag
     3181 cagactttgc agtctgtgga ctgcaacaca acattgcctt tatgtgtaac tcttggctga
     3241 agctcttaca ccaatgctgg gggacatgta cctcccaggg gcccaggaag actacgggag
     3301 gctacaccaa cgtcaatcag aggggcctgt gtagctaccg ataagcggac cctcaagagg
     3361 gcattagcaa tagtgtttat aaggccccct tgttaaccct aaacgggtag catatgcttc
     3421 ccgggtagta gtatatacta tccagactaa ccctaattca atagcatatg ttacccaacg
     3481 ggaagcatat gctatcgaat tagggttagt aaaagggtcc taaggaacag cgatatctcc
     3541 caccccatga gctgtcacgg ttttatttac atggggtcag gattccacga gggtagtgaa
     3601 ccattttagt cacaagggca gtggctgaag atcaaggagc gggcagtgaa ctctcctgaa
     3661 tcttcgcctg cttcttcatt ctccttcgtt tagctaatag aataactgct gagttgtgaa
     3721 cagtaaggtg tatgtgaggt gctcgaaaac aaggtttcag gtgacgcccc cagaataaaa
     3781 tttggacggg gggttcagtg gtggcattgt gctatgacac caatataacc ctcacaaacc
     3841 ccttgggcaa taaatactag tgtaggaatg aaacattctg aatatcttta acaatagaaa
     3901 tccatggggt ggggacaagc cgtaaagact ggatgtccat ctcacacgaa tttatggcta
     3961 tgggcaacac ataatcctag tgcaatatga tactggggtt attaagatgt gtcccaggca
     4021 gggaccaaga caggtgaacc atgttgttac actctatttg taacaagggg aaagagagtg
     4081 gacgccgaca gcagcggact ccactggttg tctctaacac ccccgaaaat taaacggggc
     4141 tccacgccaa tggggcccat aaacaaagac aagtggccac tctttttttt gaaattgtgg
     4201 agtgggggca cgcgtcagcc cccacacgcc gccctgcggt tttggactgt aaaataaggg
     4261 tgtaataact tggctgattg taaccccgct aaccactgcg gtcaaaccac ttgcccacaa
     4321 aaccactaat ggcaccccgg ggaatacctg cataagtagg tgggcgggcc aagatagggg
     4381 cgcgattgct gcgatctgga ggacaaatta cacacacttg cgcctgagcg ccaagcacag
     4441 ggttgttggt cctcatattc acgaggtcgc tgagagcacg gtgggctaat gttgccatgg
     4501 gtagcatata ctacccaaat atctggatag catatgctat cctaatctat atctgggtag
     4561 cataggctat cctaatctat atctgggtag catatgctat cctaatctat atctgggtag
     4621 tatatgctat cctaatttat atctgggtag cataggctat cctaatctat atctgggtag
     4681 catatgctat cctaatctat atctgggtag tatatgctat cctaatctgt atccgggtag
     4741 catatgctat cctaatagag attagggtag tatatgctat cctaatttat atctgggtag
     4801 catatactac ccaaatatct ggatagcata tgctatccta atctatatct gggtagcata
     4861 tgctatccta atctatatct gggtagcata ggctatccta atctatatct gggtagcata
     4921 tgctatccta atctatatct gggtagtata tgctatccta atttatatct gggtagcata
     4981 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     5041 tgctatccta atctgtatcc gggtagcata tgctatcctc atgcatatac agtcagcata
     5101 tgatacccag tagtagagtg ggagtgctat cctttgcata tgccgccacc tcccaagggg
     5161 gcgtgaattt tcgctgcttg tccttttcct gctggttgct cccattctta ggtgaattta
     5221 aggaggccag gctaaagccg tcgcatgtct gattgctcac caggtaaatg tcgctaatgt
     5281 tttccaacgc gagaaggtgt tgagcgcgga gctgagtgac gtgacaacat gggtatgccc
     5341 aattgcccca tgttgggagg acgaaaatgg tgacaagaca gatggccaga aatacaccaa
     5401 cagcacgcat gatgtctact ggggatttat tctttagtgc gggggaatac acggctttta
     5461 atacgattga gggcgtctcc taacaagtta catcactcct gcccttcctc accctcatct
     5521 ccatcacctc cttcatctcc gtcatctccg tcatcaccct ccgcggcagc cccttccacc
     5581 ataggtggaa accagggagg caaatctact ccatcgtcaa agctgcacac agtcaccctg
     5641 atattgcagg taggagcggg ctttgtcata acaaggtcct taatcgcatc cttcaaaacc
     5701 tcagcaaata tatgagtttg taaaaagacc atgaaataac agacaatgga ctcccttagc
     5761 gggccaggtt gtgggccggg tccaggggcc attccaaagg ggagacgact caatggtgta
     5821 agacgacatt gtggaatagc aagggcagtt cctcgcctta ggttgtaaag ggaggtctta
     5881 ctacctccat atacgaacac accggcgacc caagttcctt cgtcggtagt cctttctacg
     5941 tgactcctag ccaggagagc tcttaaacct tctgcaatgt tctcaaattt cgggttggaa
     6001 cctccttgac cacgatgctt tccaaaccac cctccttttt tgcgcctgcc tccatcaccc
     6061 tgaccccggg gtccagtgct tgggccttct cctgggtcat ctgcggggcc ctgctctatc
     6121 gctcccgggg gcacgtcagg ctcaccatct gggccacctt cttggtggta ttcaaaataa
     6181 tcggcttccc ctacagggtg gaaaaatggc cttctacctg gagggggcct gcgcggtgga
     6241 gacccggatg atgatgactg actactggga ctcctgggcc tcttttctcc acgtccacga
     6301 cctctccccc tggctctttc acgacttccc cccctggctc tttcacgtcc tctaccccgg
     6361 cggcctccac tacctcctcg accccggcct ccactacctc ctcgaccccg gcctccactg
     6421 cctcctcgac cccggcctcc acctcctgct cctgcccctc ctgctcctgc ccctcctcct
     6481 gctcctgccc ctcctgcccc tcctgctcct gcccctcctg cccctcctgc tcctgcccct
     6541 cctgcccctc ctgctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctgcccct
     6601 cctcctgctc ctgcccctcc tgcccctcct gctcctgccc ctcctgcccc tcctgctcct
     6661 gcccctcctg cccctcctgc tcctgcccct cctgctcctg cccctcctgc tcctgcccct
     6721 cctgctcctg cccctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctgctcct
     6781 gcccctcctg cccctcctgc ccctcctgct cctgcccctc ctcctgctcc tgcccctcct
     6841 gcccctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctcctgc tcctgcccct
     6901 cctcctgctc ctgcccctcc tgcccctcct gcccctcctc ctgctcctgc ccctcctgcc
     6961 cctcctcctg ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc tgcccctcct
     7021 cctgctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctgcccc tcctgcccct
     7081 cctcctgctc ctgcccctcc tcctgctcct gcccctcctg ctcctgcccc tcccgctcct
     7141 gctcctgctc ctgttccacc gtgggtccct ttgcagccaa tgcaacttgg acgtttttgg
     7201 ggtctccgga caccatctct atgtcttggc cctgatcctg agccgcccgg ggctcctggt
     7261 cttccgcctc ctcgtcctcg tcctcttccc cgtcctcgtc catggttatc accccctctt
     7321 ctttgaggtc cactgccgcc ggagccttct ggtccagatg tgtctccctt ctctcctagg
     7381 ccatttccag gtcctgtacc tggcccctcg tcagacatga ttcacactaa aagagatcaa
     7441 tagacatctt tattagacga cgctcagtga atacagggag tgcagactcc tgccccctcc
     7501 aacagccccc ccaccctcat ccccttcatg gtcgctgtca gacagatcca ggtctgaaaa
     7561 ttccccatcc tccgaaccat cctcgtcctc atcaccaatt actcgcagcc cggaaaactc
     7621 ccgctgaaca tcctcaagat ttgcgtcctg agcctcaagc caggcctcaa attcctcgtc
     7681 cccctttttg ctggacggta gggatgggga ttctcgggac ccctcctctt cctcttcaag
     7741 gtcaccagac agagatgcta ctggggcaac ggaagaaaag ctgggtgcgg cctgtgagga
     7801 tcagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg
     7861 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     7921 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     7981 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     8041 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     8101 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     8161 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     8221 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     8281 tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     8341 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     8401 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     8461 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     8521 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     8581 atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     8641 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     8701 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     8761 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     8821 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     8881 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     8941 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     9001 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     9061 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     9121 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     9181 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     9241 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     9301 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     9361 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     9421 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     9481 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     9541 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     9601 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     9661 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttgaagctgt
     9721 ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg
     9781 ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc
     9841 agcaagacgt agcccagcgc gtcggccccg agatgcgccg cgtgcggctg ctggagatgg
     9901 cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga
     9961 attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cctgcttcat
    10021 ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg gaagaaatat atttgcatgt
    10081 ctttagttct atgatgacac aaaccccgcc cagcgtcttg tcattggcga attcgaacac
    10141 gcagatgcag tcggggcggc gcggtccgag gtccacttcg catattaagg tgacgcgtgt
    10201 ggcctcgaac accgagcgac cctgcagcga cccgcttaac agcgtcaaca gcgtgccgca
    10261 gatcccgggg ggcaatgaga tatgaaaaag cctgaactca ccgcgacgtc tgtcgagaag
    10321 tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa
    10381 tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc
    10441 gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg
    10501 attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg catctcccgc
    10561 cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctgcag
    10621 ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc
    10681 ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg
    10741 attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc
    10801 gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac
    10861 ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg
    10921 gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc
    10981 ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat
    11041 ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa
    11101 ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc
    11161 gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc
    11221 gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc
    11281 agcactcgtc cggatcggga gatgggggag gctaactgaa acacggaagg agacaatacc
    11341 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg ggtgttgggt
    11401 cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga
    11461 gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt
    11521 cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc atagccactg
    11581 gccccgtggg ttagggacgg ggtcccccat ggggaatggt ttatggttcg tgggggttat
    11641 tattttgggc gttgcgtggg gtcaggtcca cgactggact gagcagacag acccatggtt
    11701 tttggatggc ctgggcatgg accgcatgta ctggcgcgac acgaacaccg ggcgtctgtg
    11761 gctgccaaac acccccgacc cccaaaaacc accgcgcgga tttctggcgt gccaagctag
    11821 tcgaccaatt ctcatgtttg acagcttatc atcgcagatc cgggcaacgt tgttgccatt
    11881 gctgcaggcg cagaactggt aggtatggaa gatctataca ttgaatcaat attggcaatt
    11941 agccatatta gtcattggtt atatagcata aatcaatatt ggctattggc cattgcatac
    12001 gttgtatcta tatcataata tgtacattta tattggctca tgtccaatat gaccgccatg
    12061 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
    12121 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
    12181 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
    12241 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
    12301 tcaagtgtat catatgccaa gtccgccccc tattgacgtc aatgacggta aatggcccgc
    12361 ctggcattat gcccagtaca tgaccttacg ggactttcct acttggcagt acatctacgt
    12421 attagtcatc gctattacca tggtgatgcg gttttggcag tacaccaatg ggcgtggata
    12481 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
    12541 ttggcaccaa aatcaacggg actttccaaa atgtcgtaat aaccccgccc cgttgacgca
    12601 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg
    12661 tcagatctct agaagctggg taccagctgc tagc
//
