LOCUS       sequence-23522-e         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Zhou BY, Ye Z, Chen G, Gao ZP, Zhang YA, Cheng L
  TITLE     Inducible and reversible transgene expression in human stem cells 
            after efficient and stable gene transfer.
  JOURNAL   Stem Cells. 2007 Mar . 25(3):779-89.
  PUBMED    17158240
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cells.
            2007 Mar . 25(3):779-89."
COMMENT     Sequence Label: iDuet101a
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(158..177)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(159..177)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     intron          203..406
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     complement(226..246)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 aaagctcctc gcccttgctc accatggntg gcgaccggta cccggggatc ctctagactc
       61 gagcggccgc cactgntgct ggatatctgc agaattccac cacactggac tagtggatcc
      121 gagctcggta ccaagcttac ctagccagct tgggtctccc tatagtgagt cgtattagta
      181 ccaagctaat tcctcacgac acctgaaatg gaagaaaaaa actttgaacc actgtctgag
      241 gcttgagaat gaaccaagat ccaaactcaa aaagggcaaa ttccaaggag aattacatca
      301 agtgccaagc tggcctaact tcagtctcca cccactcagt gtggggaaac tccatcgcat
      361 aaaacccctc cccccaacct aaagacgacg tactccaaaa gctcgagaac taatcgaggt
      421 gcctggacgg cgcccggtac tccgtggagt cacatgaagc gacggctgag gacggaaagg
      481 cccttttcct ttgtgtgggt gactcacccg cccgctctcc cgagcgccgc gtcctccatt
      541 ttgagctccc tgcagcaggg ccgggaagcg gccatctttc cgctcacgca actggtgccg
      601 accgggccag ccttgccgcc cagggcgggg cgatacacgg cggcgcgagg ccaggcacca
      661 gagcaggccg gccagcttga gactaccccc gtccgattct cggtggccgc gctcgcaggc
      721 cccgcctcgc cgaacatgtg cgctgggacg cacgggcccc gtcgccgccc gcggcccaaa
      781 accgaantac ngtgtgcaat ctgggccgca ttacaaactt cttgccanaa aaagngtcnc
      841 agcggtctca aaatttaatg gntnaaattt cgaagcg
//
