LOCUS       sequence-235225-       12730 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the AD8 HIV-1 isolate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12730)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 12730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-AD8gp160
FEATURES             Location/Qualifiers
     source          1..12730
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1948..1992
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     polyA_signal    2579..2713
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2603..2622)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2657..2676
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2856..2878)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      3438..5227
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(5529..7454)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(7889..7907)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7975..8079
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8080..8148,8149..8940)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8298..8317)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9111..9699
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9600..9619
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        10130..10275
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             10318..11355
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(11353..11372)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11397..11445
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        12099..12478
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12479..12682
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     12633..12653
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     12679..12703
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gccaccatgc caatgggttc actgcaacca cttgcgacgc tgtacctctt gggcatgctg
       61 gtggcatccg ttctggcagt tgagaatctc tgggtcactg tttactacgg cgtgcccgta
      121 tggaaggaag ccacgacgac attgttctgt gcgagcgatg cgaaggcata cgacactgag
      181 gtccacaatg tctgggcaac acacgcgtgc gtccctaccg accccaatcc ccaggaagtg
      241 gtccttgaaa atgtcactga gaattttaat atgtggaaga ataatatggt tgagcagatg
      301 catgaggaca tcatttctct gtgggatcag tccctcaaac cttgtgtcaa gttgacgcct
      361 ctgtgtgtaa ccctcaactg caccgatttg aggaatgtaa ccaatatcaa caattctagt
      421 gaaggtatga ggggagagat caaaaactgc tccttcaaca taaccacctc cattcgagac
      481 aaggtaaaaa aggattacgc tctcttttat agattggacg tggtacccat tgacaatgat
      541 aacaccagtt ataggctgat aaactgtaat acctcaacga ttacacaagc ttgtcctaaa
      601 gtatcattcg aacccattcc gatccattac tgtacgccgg ccggtttcgc catccttaaa
      661 tgtaaggata agaaattcaa tggtactggc ccgtgcaaaa atgtatctac ggtccagtgt
      721 acacacggca taagacccgt agtatctacg caactcctgc ttaacgggag cctggccgaa
      781 gaggaagttg taatcagaag tagtaacttc actgataatg ccaagaacat cattgtgcag
      841 ttgaaagagt cagtcgagat caactgtacc aggccgaaca ataatacacg aaagtcaata
      901 cacatagggc ccggaagggc attctacacc actggggata taatcggaga tatacgccaa
      961 gcgcattgca atatcagtag gaccaagtgg aacaatacgc tgaaccaaat cgctacaaag
     1021 cttaaggaac aatttgggaa taacaagaca atagtattta atcaatcatc tgggggcgac
     1081 ccggagatag tcatgcattc atttaattgc ggcggagaat ttttttattg taattccaca
     1141 cagcttttta atagcacatg gaatttcaac ggaacatgga atctgacaca gtccaatggt
     1201 acggagggga atgacacaat tacccttccc tgtcgcatta aacagataat taatatgtgg
     1261 caagaagtcg gaaaggcgat gtatgctcct cctattcgag ggcagataag gtgctcatca
     1321 aatatcaccg gcctcatact gactcgggac ggcggaaata atcacaacaa tgacactgag
     1381 acatttcgcc caggtggagg tgatatgcgg gataactggc gctccgaatt gtacaagtac
     1441 aaagttgtca agattgagcc actcggagtg gccccgacaa aggctaagag gcgggtcgta
     1501 caaagggaga aacgcgcggt aggaaccatt ggagccatgt tcttgggttt cttgggcgca
     1561 gcgggatcca ccatgggggc ggcctctatc acactgaccg tgcaggcaag actcctcttg
     1621 agcggcatcg tgcaacagca aaacaatctc ttgcgcgcta ttgaggctca acagcacctg
     1681 cttcaactta ccgtatgggg catcaaacaa ttgcaggctc gggtcttggc tgtcgagagg
     1741 tatttgcggg atcagcaact tttgggtatc tggggttgtt ccgggaagct catctgcaca
     1801 acggctgtcc catggaatgc cagttggtcc aacaagaccc tggacatgat ctggaataac
     1861 atgacttgga tggagtggga gagagaaatc gacaactaca cgggactcat ctataccttg
     1921 attgaggagt ctcagaacca acaagagaag aacgagcagg aactgctgga gttggacaag
     1981 tgggccagtc tgtggaattg gttcgatata accaactggc tttggtacat caaaatattt
     2041 ataatgatcg ttggtggtct gattggtctt aggattgttt tcaccgtcct gagcattgtc
     2101 aaccgcgtga ggcagggtta ttctcccctt tcctttcaaa ctcacttgcc cgcccctagg
     2161 gggccagacc gccctgaggg gattgaagag gagggcggcg accgcgaccg agataggagt
     2221 gtgcggctcg tggatgggtt tctggccctc ttctgggatg accttcggtc cctctgtctt
     2281 ttctcctatc atcgcctgag ggatcttctt ttgattgtag ctaggatcgt cgagctgttg
     2341 gggcgccggg gatgggaggc ccttaaatac tggtggaatt tgttgcaata ttggtcccaa
     2401 gagctcagga acagtgcggt gtctcttttg aacgcgacag ccatcgcagt agccgaggga
     2461 accgaccgag ttatagagat agtccaacgg atctaccgag ctatcctcca catcccaacg
     2521 agaattcgac aaggccttga gcggctcctt ctttagtaac tcgaggccgg caaggccgga
     2581 tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     2641 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     2701 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
     2761 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatcc
     2821 ggctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     2881 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     2941 gcgggtgttg gcgggtgtcg gggcgcagcc atgaggtcga ctctagagga tcgatccccg
     3001 ccccggacga actaaacctg actacgacat ctctgcccct tcttcgcggg gcagtgcatg
     3061 taatcccttc agttggttgg tacaacttgc caactgggcc ctgttccaca tgtgacacgg
     3121 ggggggacca aacacaaagg ggttctctga ctgtagttga catccttata aatggatgtg
     3181 cacatttgcc aacactgagt ggctttcatc ctggagcaga ctttgcagtc tgtggactgc
     3241 aacacaacat tgcctttatg tgtaactctt ggctgaagct cttacaccaa tgctggggga
     3301 catgtacctc ccaggggccc aggaagacta cgggaggcta caccaacgtc aatcagaggg
     3361 gcctgtgtag ctaccgataa gcggaccctc aagagggcat tagcaatagt gtttataagg
     3421 cccccttgtt aaccctaaac gggtagcata tgcttcccgg gtagtagtat atactatcca
     3481 gactaaccct aattcaatag catatgttac ccaacgggaa gcatatgcta tcgaattagg
     3541 gttagtaaaa gggtcctaag gaacagcgat atctcccacc ccatgagctg tcacggtttt
     3601 atttacatgg ggtcaggatt ccacgagggt agtgaaccat tttagtcaca agggcagtgg
     3661 ctgaagatca aggagcgggc agtgaactct cctgaatctt cgcctgcttc ttcattctcc
     3721 ttcgtttagc taatagaata actgctgagt tgtgaacagt aaggtgtatg tgaggtgctc
     3781 gaaaacaagg tttcaggtga cgcccccaga ataaaatttg gacggggggt tcagtggtgg
     3841 cattgtgcta tgacaccaat ataaccctca caaacccctt gggcaataaa tactagtgta
     3901 ggaatgaaac attctgaata tctttaacaa tagaaatcca tggggtgggg acaagccgta
     3961 aagactggat gtccatctca cacgaattta tggctatggg caacacataa tcctagtgca
     4021 atatgatact ggggttatta agatgtgtcc caggcaggga ccaagacagg tgaaccatgt
     4081 tgttacactc tatttgtaac aaggggaaag agagtggacg ccgacagcag cggactccac
     4141 tggttgtctc taacaccccc gaaaattaaa cggggctcca cgccaatggg gcccataaac
     4201 aaagacaagt ggccactctt ttttttgaaa ttgtggagtg ggggcacgcg tcagccccca
     4261 cacgccgccc tgcggttttg gactgtaaaa taagggtgta ataacttggc tgattgtaac
     4321 cccgctaacc actgcggtca aaccacttgc ccacaaaacc actaatggca ccccggggaa
     4381 tacctgcata agtaggtggg cgggccaaga taggggcgcg attgctgcga tctggaggac
     4441 aaattacaca cacttgcgcc tgagcgccaa gcacagggtt gttggtcctc atattcacga
     4501 ggtcgctgag agcacggtgg gctaatgttg ccatgggtag catatactac ccaaatatct
     4561 ggatagcata tgctatccta atctatatct gggtagcata ggctatccta atctatatct
     4621 gggtagcata tgctatccta atctatatct gggtagtata tgctatccta atttatatct
     4681 gggtagcata ggctatccta atctatatct gggtagcata tgctatccta atctatatct
     4741 gggtagtata tgctatccta atctgtatcc gggtagcata tgctatccta atagagatta
     4801 gggtagtata tgctatccta atttatatct gggtagcata tactacccaa atatctggat
     4861 agcatatgct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     4921 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     4981 agtatatgct atcctaattt atatctgggt agcataggct atcctaatct atatctgggt
     5041 agcatatgct atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt
     5101 agcatatgct atcctcatgc atatacagtc agcatatgat acccagtagt agagtgggag
     5161 tgctatcctt tgcatatgcc gccacctccc aagggggcgt gaattttcgc tgcttgtcct
     5221 tttcctgctg gttgctccca ttcttaggtg aatttaagga ggccaggcta aagccgtcgc
     5281 atgtctgatt gctcaccagg taaatgtcgc taatgttttc caacgcgaga aggtgttgag
     5341 cgcggagctg agtgacgtga caacatgggt atgcccaatt gccccatgtt gggaggacga
     5401 aaatggtgac aagacagatg gccagaaata caccaacagc acgcatgatg tctactgggg
     5461 atttattctt tagtgcgggg gaatacacgg cttttaatac gattgagggc gtctcctaac
     5521 aagttacatc actcctgccc ttcctcaccc tcatctccat cacctccttc atctccgtca
     5581 tctccgtcat caccctccgc ggcagcccct tccaccatag gtggaaacca gggaggcaaa
     5641 tctactccat cgtcaaagct gcacacagtc accctgatat tgcaggtagg agcgggcttt
     5701 gtcataacaa ggtccttaat cgcatccttc aaaacctcag caaatatatg agtttgtaaa
     5761 aagaccatga aataacagac aatggactcc cttagcgggc caggttgtgg gccgggtcca
     5821 ggggccattc caaaggggag acgactcaat ggtgtaagac gacattgtgg aatagcaagg
     5881 gcagttcctc gccttaggtt gtaaagggag gtcttactac ctccatatac gaacacaccg
     5941 gcgacccaag ttccttcgtc ggtagtcctt tctacgtgac tcctagccag gagagctctt
     6001 aaaccttctg caatgttctc aaatttcggg ttggaacctc cttgaccacg atgctttcca
     6061 aaccaccctc cttttttgcg cctgcctcca tcaccctgac cccggggtcc agtgcttggg
     6121 ccttctcctg ggtcatctgc ggggccctgc tctatcgctc ccgggggcac gtcaggctca
     6181 ccatctgggc caccttcttg gtggtattca aaataatcgg cttcccctac agggtggaaa
     6241 aatggccttc tacctggagg gggcctgcgc ggtggagacc cggatgatga tgactgacta
     6301 ctgggactcc tgggcctctt ttctccacgt ccacgacctc tccccctggc tctttcacga
     6361 cttccccccc tggctctttc acgtcctcta ccccggcggc ctccactacc tcctcgaccc
     6421 cggcctccac tacctcctcg accccggcct ccactgcctc ctcgaccccg gcctccacct
     6481 cctgctcctg cccctcctgc tcctgcccct cctcctgctc ctgcccctcc tgcccctcct
     6541 gctcctgccc ctcctgcccc tcctgctcct gcccctcctg cccctcctgc tcctgcccct
     6601 cctgcccctc ctcctgctcc tgcccctcct gcccctcctc ctgctcctgc ccctcctgcc
     6661 cctcctgctc ctgcccctcc tgcccctcct gctcctgccc ctcctgcccc tcctgctcct
     6721 gcccctcctg ctcctgcccc tcctgctcct gcccctcctg ctcctgcccc tcctgcccct
     6781 cctgcccctc ctcctgctcc tgcccctcct gctcctgccc ctcctgcccc tcctgcccct
     6841 cctgctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctgcccc tcctcctgct
     6901 cctgcccctc ctgcccctcc tcctgctcct gcccctcctc ctgctcctgc ccctcctgcc
     6961 cctcctgccc ctcctcctgc tcctgcccct cctgcccctc ctcctgctcc tgcccctcct
     7021 cctgctcctg cccctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctcctgct
     7081 cctgcccctc ctgcccctcc tgcccctcct gcccctcctc ctgctcctgc ccctcctcct
     7141 gctcctgccc ctcctgctcc tgcccctccc gctcctgctc ctgctcctgt tccaccgtgg
     7201 gtccctttgc agccaatgca acttggacgt ttttggggtc tccggacacc atctctatgt
     7261 cttggccctg atcctgagcc gcccggggct cctggtcttc cgcctcctcg tcctcgtcct
     7321 cttccccgtc ctcgtccatg gttatcaccc cctcttcttt gaggtccact gccgccggag
     7381 ccttctggtc cagatgtgtc tcccttctct cctaggccat ttccaggtcc tgtacctggc
     7441 ccctcgtcag acatgattca cactaaaaga gatcaataga catctttatt agacgacgct
     7501 cagtgaatac agggagtgca gactcctgcc ccctccaaca gcccccccac cctcatcccc
     7561 ttcatggtcg ctgtcagaca gatccaggtc tgaaaattcc ccatcctccg aaccatcctc
     7621 gtcctcatca ccaattactc gcagcccgga aaactcccgc tgaacatcct caagatttgc
     7681 gtcctgagcc tcaagccagg cctcaaattc ctcgtccccc tttttgctgg acggtaggga
     7741 tggggattct cgggacccct cctcttcctc ttcaaggtca ccagacagag atgctactgg
     7801 ggcaacggaa gaaaagctgg gtgcggcctg tgaggatcag cttatcgatg ataagctgtc
     7861 aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     7921 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     7981 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     8041 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     8101 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     8161 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     8221 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     8281 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
     8341 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     8401 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     8461 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     8521 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     8581 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg
     8641 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     8701 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     8761 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     8821 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     8881 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     8941 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     9001 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     9061 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     9121 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     9181 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     9241 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     9301 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     9361 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     9421 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     9481 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     9541 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     9601 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     9661 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     9721 ttacggttcc tggccttttg ctggccttga agctgtccct gatggtcgtc atctacctgc
     9781 ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata
     9841 atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg
     9901 gccccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc
     9961 aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag
    10021 tggtgaatcc gttagcgagg tgccgccctg cttcatcccc gtggcccgtt gctcgcgttt
    10081 gctggcggtg tccccggaag aaatatattt gcatgtcttt agttctatga tgacacaaac
    10141 cccgcccagc gtcttgtcat tggcgaattc gaacacgcag atgcagtcgg ggcggcgcgg
    10201 tccgaggtcc acttcgcata ttaaggtgac gcgtgtggcc tcgaacaccg agcgaccctg
    10261 cagcgacccg cttaacagcg tcaacagcgt gccgcagatc ccggggggca atgagatatg
    10321 aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc
    10381 gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta
    10441 ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt
    10501 tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg
    10561 gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa
    10621 gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg
    10681 atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc
    10741 ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac
    10801 tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg
    10861 atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc
    10921 aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg
    10981 ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt
    11041 atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg
    11101 ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc
    11161 aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc
    11221 gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt
    11281 gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgga tcgggagatg
    11341 ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc
    11401 aataaaaaga cagaataaaa cgcacgggtg ttgggtcgtt tgttcataaa cgcggggttc
    11461 ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc
    11521 gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag
    11581 ccaacgtcgg ggcggcaggc cctgccatag ccactggccc cgtgggttag ggacggggtc
    11641 ccccatgggg aatggtttat ggttcgtggg ggttattatt ttgggcgttg cgtggggtca
    11701 ggtccacgac tggactgagc agacagaccc atggtttttg gatggcctgg gcatggaccg
    11761 catgtactgg cgcgacacga acaccgggcg tctgtggctg ccaaacaccc ccgaccccca
    11821 aaaaccaccg cgcggatttc tggcgtgcca agctagtcga ccaattctca tgtttgacag
    11881 cttatcatcg cagatccggg caacgttgtt gccattgctg caggcgcaga actggtaggt
    11941 atggaagatc tatacattga atcaatattg gcaattagcc atattagtca ttggttatat
    12001 agcataaatc aatattggct attggccatt gcatacgttg tatctatatc ataatatgta
    12061 catttatatt ggctcatgtc caatatgacc gccatgttga cattgattat tgactagtta
    12121 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
    12181 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
    12241 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
    12301 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc
    12361 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
    12421 cttacgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
    12481 gatgcggttt tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc
    12541 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
    12601 tccaaaatgt cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg
    12661 ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atctctagaa gctgggtacc
    12721 agctgctagc
//
