LOCUS       sequence-235228-       12733 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the CH111 HIV-1 isolate; 
            QES mutant for enhanced presentation of quaternary epitopes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12733)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 12733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-CH111-QES.c13.L544Y
FEATURES             Location/Qualifiers
     source          1..12733
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    2582..2716
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2606..2625)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2660..2679
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2859..2881)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      3441..5230
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(5532..7457)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(7892..7910)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7978..8082
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8083..8151,8152..8943)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8301..8320)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9114..9702
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9603..9622
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        10133..10278
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             10321..11358
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(11356..11375)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11400..11448
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        12102..12481
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12482..12685
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     12636..12656
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     12682..12706
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gccaccatgc ccatgggtag tctgcagcct ttggctactc tttacttgtt gggtatgttg
       61 gtagcgagcg tcctcgcagt cgggaatttg tgggttacag tttattacgg ggtcccggtg
      121 tggaaggagg cgacgaccac attgttttgc gcctccgatg caaaggctta cgacacggaa
      181 gttcacaacg tttgggccac ccatgcgtgc gtgcctacgg accctaatcc acaggagatg
      241 ttcatgccca atgtcaccga aaattttaat atgtggaaaa acgacatggt aaatcaaatg
      301 catgaggatg tcataagtct ctgggaccaa agtctgaagc catgcgtgaa attgaccccg
      361 ctctgcgtga cactggagtg taaaaacgtg tcacgaaata attccagttc aaatggtact
      421 ggtacaaaca atgaagcgta tcgagaggga atgaaggaaa taaagaactg ctcttttaat
      481 gccactgccg agcttagaga ccggaagcag aacgtgtatg cactgtttta caggcttgac
      541 atcgtacctc ttgcggagaa cagtagcgaa tataggctta tcaattgcaa caccagcgca
      601 attactcaag cctgccccaa aatgactttt gacccgattc ctatacacta ctgcacacca
      661 gcagggtacg caattcttaa gtgtagcgac aaaaccttca acggcactgg cccctgttca
      721 aatgtctcca cagttcaatg tactcacgga atacgaccgg tcatgagtac acagttgctg
      781 ctgaacggtt ctctggccga agaggagatc atcatccgct ctgagaacct cacgaacaat
      841 gccaagccaa taatcgtaca gcttaaccag agcgtggaaa tagtttgcac acgccctaac
      901 aataacacta gaaagtcaat caggatcgga ccagggcaga cgttttatgc gacaggagga
      961 ataataggcg acatccgaca agcgtactgt aacatttccc ggaataaatg gaacgagaca
     1021 ttgagaatgg taggtaaaaa attggcggaa cattttccta acaaaaccat caattttaca
     1081 tcctcctccg ggggagatct ggaaatcacg acgcacagtt ttacgtgtag gggagaattc
     1141 ttctattgca acacctccgg actgtttaat ggtacgtaca tgcccaacgg tacgtatatg
     1201 ttcaactcta cgaatgccac gatcacaatc ccgtgtagaa ttaagcagat catcaatatg
     1261 tggcaagggg tcggccgagc catatatgct ccgcctatag cgggcaatat cacctgcaaa
     1321 tccaacataa cgggtcttct tcttgtacgc gacggtggag aaacaaacga taccaatacc
     1381 accgagacat ttagaccagg cggaggagat atgcgagata attggcggag cgaattgtac
     1441 aagtacaagg tggtcgagat aaagcctttg ggtgtggcac ccacgggcgc aaagcgcaga
     1501 atagtcggaa gagaaaagag agcagttggg ctcggcgctg cttttcttgg tttcctcagc
     1561 gttgccggta gtacgatggg agcagcgtca atgaccctga ctgtccaagc gcgacagtac
     1621 cttagcggaa tagtgcaaca acagagcaac ctgctgcggg caattgaagc acagcagcat
     1681 ttgctccaac tgactgtctg gggtataaag caactgcagg ccagggtttt ggccatagag
     1741 agatacctca aagatcaaca gctcctgggt atctgggggt gttcaggaaa gctcatctgt
     1801 acgaccacag taccctggaa ctcttcctgg agcaataaaa accttactga gatctgggat
     1861 aatatgactt ggatgcagtg ggacaaggaa atctccaact acaccaatac gatttattgg
     1921 ttgcttgagg agtcacagaa tcagcaagag aagaacgaaa aggatctcct cgccctcgat
     1981 tcatggaaca atttgtggaa ctggttcgat atttctaaat ggctgtggta catcaagata
     2041 ttcataatga tcgtcggcgg acttatcggt ctccgaatca tatttgcggt actcagtatc
     2101 gtgaacaggg tacggcaagg gtacagtccc cttagctttc aaacacttac cccgaaccct
     2161 ggcggacctg acaggcttgg acgaatcgag gaggaaggag gagaacaaga tcgccgcagg
     2221 tctattagac tggtatccgg cttcctcgcg cttgcgtggg acgatttgcg aaacttgtgt
     2281 ttgtttagtt atcatcagtt gagggatttc atcctcgtcg cggcgcgcgt cgtcgagctt
     2341 ttgggtcaaa gaggatggga agcgctcaag tacctcggca gtttggtaca atattggggg
     2401 caagaattga aaaaatctgc tatctcactg attgatacta ttgccatagc cgttgctgaa
     2461 ggaactgatc ggattataga gttgatccag ggcctttgcc gcgctatata ccatattcca
     2521 cgaaggatcc ggcagggatt tgaggcagcg ttgcaatagt aactcgaggc cggcaaggcc
     2581 ggatccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     2641 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     2701 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     2761 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga
     2821 tccggctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     2881 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     2941 tcagcgggtg ttggcgggtg tcggggcgca gccatgaggt cgactctaga ggatcgatcc
     3001 ccgccccgga cgaactaaac ctgactacga catctctgcc ccttcttcgc ggggcagtgc
     3061 atgtaatccc ttcagttggt tggtacaact tgccaactgg gccctgttcc acatgtgaca
     3121 cgggggggga ccaaacacaa aggggttctc tgactgtagt tgacatcctt ataaatggat
     3181 gtgcacattt gccaacactg agtggctttc atcctggagc agactttgca gtctgtggac
     3241 tgcaacacaa cattgccttt atgtgtaact cttggctgaa gctcttacac caatgctggg
     3301 ggacatgtac ctcccagggg cccaggaaga ctacgggagg ctacaccaac gtcaatcaga
     3361 ggggcctgtg tagctaccga taagcggacc ctcaagaggg cattagcaat agtgtttata
     3421 aggccccctt gttaacccta aacgggtagc atatgcttcc cgggtagtag tatatactat
     3481 ccagactaac cctaattcaa tagcatatgt tacccaacgg gaagcatatg ctatcgaatt
     3541 agggttagta aaagggtcct aaggaacagc gatatctccc accccatgag ctgtcacggt
     3601 tttatttaca tggggtcagg attccacgag ggtagtgaac cattttagtc acaagggcag
     3661 tggctgaaga tcaaggagcg ggcagtgaac tctcctgaat cttcgcctgc ttcttcattc
     3721 tccttcgttt agctaataga ataactgctg agttgtgaac agtaaggtgt atgtgaggtg
     3781 ctcgaaaaca aggtttcagg tgacgccccc agaataaaat ttggacgggg ggttcagtgg
     3841 tggcattgtg ctatgacacc aatataaccc tcacaaaccc cttgggcaat aaatactagt
     3901 gtaggaatga aacattctga atatctttaa caatagaaat ccatggggtg gggacaagcc
     3961 gtaaagactg gatgtccatc tcacacgaat ttatggctat gggcaacaca taatcctagt
     4021 gcaatatgat actggggtta ttaagatgtg tcccaggcag ggaccaagac aggtgaacca
     4081 tgttgttaca ctctatttgt aacaagggga aagagagtgg acgccgacag cagcggactc
     4141 cactggttgt ctctaacacc cccgaaaatt aaacggggct ccacgccaat ggggcccata
     4201 aacaaagaca agtggccact cttttttttg aaattgtgga gtgggggcac gcgtcagccc
     4261 ccacacgccg ccctgcggtt ttggactgta aaataagggt gtaataactt ggctgattgt
     4321 aaccccgcta accactgcgg tcaaaccact tgcccacaaa accactaatg gcaccccggg
     4381 gaatacctgc ataagtaggt gggcgggcca agataggggc gcgattgctg cgatctggag
     4441 gacaaattac acacacttgc gcctgagcgc caagcacagg gttgttggtc ctcatattca
     4501 cgaggtcgct gagagcacgg tgggctaatg ttgccatggg tagcatatac tacccaaata
     4561 tctggatagc atatgctatc ctaatctata tctgggtagc ataggctatc ctaatctata
     4621 tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc ctaatttata
     4681 tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata
     4741 tctgggtagt atatgctatc ctaatctgta tccgggtagc atatgctatc ctaatagaga
     4801 ttagggtagt atatgctatc ctaatttata tctgggtagc atatactacc caaatatctg
     4861 gatagcatat gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     4921 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     4981 ggtagtatat gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg
     5041 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg
     5101 ggtagcatat gctatcctca tgcatataca gtcagcatat gatacccagt agtagagtgg
     5161 gagtgctatc ctttgcatat gccgccacct cccaaggggg cgtgaatttt cgctgcttgt
     5221 ccttttcctg ctggttgctc ccattcttag gtgaatttaa ggaggccagg ctaaagccgt
     5281 cgcatgtctg attgctcacc aggtaaatgt cgctaatgtt ttccaacgcg agaaggtgtt
     5341 gagcgcggag ctgagtgacg tgacaacatg ggtatgccca attgccccat gttgggagga
     5401 cgaaaatggt gacaagacag atggccagaa atacaccaac agcacgcatg atgtctactg
     5461 gggatttatt ctttagtgcg ggggaataca cggcttttaa tacgattgag ggcgtctcct
     5521 aacaagttac atcactcctg cccttcctca ccctcatctc catcacctcc ttcatctccg
     5581 tcatctccgt catcaccctc cgcggcagcc ccttccacca taggtggaaa ccagggaggc
     5641 aaatctactc catcgtcaaa gctgcacaca gtcaccctga tattgcaggt aggagcgggc
     5701 tttgtcataa caaggtcctt aatcgcatcc ttcaaaacct cagcaaatat atgagtttgt
     5761 aaaaagacca tgaaataaca gacaatggac tcccttagcg ggccaggttg tgggccgggt
     5821 ccaggggcca ttccaaaggg gagacgactc aatggtgtaa gacgacattg tggaatagca
     5881 agggcagttc ctcgccttag gttgtaaagg gaggtcttac tacctccata tacgaacaca
     5941 ccggcgaccc aagttccttc gtcggtagtc ctttctacgt gactcctagc caggagagct
     6001 cttaaacctt ctgcaatgtt ctcaaatttc gggttggaac ctccttgacc acgatgcttt
     6061 ccaaaccacc ctcctttttt gcgcctgcct ccatcaccct gaccccgggg tccagtgctt
     6121 gggccttctc ctgggtcatc tgcggggccc tgctctatcg ctcccggggg cacgtcaggc
     6181 tcaccatctg ggccaccttc ttggtggtat tcaaaataat cggcttcccc tacagggtgg
     6241 aaaaatggcc ttctacctgg agggggcctg cgcggtggag acccggatga tgatgactga
     6301 ctactgggac tcctgggcct cttttctcca cgtccacgac ctctccccct ggctctttca
     6361 cgacttcccc ccctggctct ttcacgtcct ctaccccggc ggcctccact acctcctcga
     6421 ccccggcctc cactacctcc tcgaccccgg cctccactgc ctcctcgacc ccggcctcca
     6481 cctcctgctc ctgcccctcc tgctcctgcc cctcctcctg ctcctgcccc tcctgcccct
     6541 cctgctcctg cccctcctgc ccctcctgct cctgcccctc ctgcccctcc tgctcctgcc
     6601 cctcctgccc ctcctcctgc tcctgcccct cctgcccctc ctcctgctcc tgcccctcct
     6661 gcccctcctg ctcctgcccc tcctgcccct cctgctcctg cccctcctgc ccctcctgct
     6721 cctgcccctc ctgctcctgc ccctcctgct cctgcccctc ctgctcctgc ccctcctgcc
     6781 cctcctgccc ctcctcctgc tcctgcccct cctgctcctg cccctcctgc ccctcctgcc
     6841 cctcctgctc ctgcccctcc tcctgctcct gcccctcctg cccctcctgc ccctcctcct
     6901 gctcctgccc ctcctgcccc tcctcctgct cctgcccctc ctcctgctcc tgcccctcct
     6961 gcccctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctcctgc tcctgcccct
     7021 cctcctgctc ctgcccctcc tgcccctcct gcccctcctc ctgctcctgc ccctcctcct
     7081 gctcctgccc ctcctgcccc tcctgcccct cctgcccctc ctcctgctcc tgcccctcct
     7141 cctgctcctg cccctcctgc tcctgcccct cccgctcctg ctcctgctcc tgttccaccg
     7201 tgggtccctt tgcagccaat gcaacttgga cgtttttggg gtctccggac accatctcta
     7261 tgtcttggcc ctgatcctga gccgcccggg gctcctggtc ttccgcctcc tcgtcctcgt
     7321 cctcttcccc gtcctcgtcc atggttatca ccccctcttc tttgaggtcc actgccgccg
     7381 gagccttctg gtccagatgt gtctcccttc tctcctaggc catttccagg tcctgtacct
     7441 ggcccctcgt cagacatgat tcacactaaa agagatcaat agacatcttt attagacgac
     7501 gctcagtgaa tacagggagt gcagactcct gccccctcca acagcccccc caccctcatc
     7561 cccttcatgg tcgctgtcag acagatccag gtctgaaaat tccccatcct ccgaaccatc
     7621 ctcgtcctca tcaccaatta ctcgcagccc ggaaaactcc cgctgaacat cctcaagatt
     7681 tgcgtcctga gcctcaagcc aggcctcaaa ttcctcgtcc ccctttttgc tggacggtag
     7741 ggatggggat tctcgggacc cctcctcttc ctcttcaagg tcaccagaca gagatgctac
     7801 tggggcaacg gaagaaaagc tgggtgcggc ctgtgaggat cagcttatcg atgataagct
     7861 gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt
     7921 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     7981 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     8041 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     8101 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     8161 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     8221 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     8281 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa
     8341 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     8401 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     8461 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     8521 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     8581 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca
     8641 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     8701 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     8761 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     8821 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     8881 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     8941 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     9001 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     9061 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     9121 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     9181 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     9241 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     9301 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     9361 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     9421 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     9481 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     9541 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     9601 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     9661 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     9721 tttttacggt tcctggcctt ttgctggcct tgaagctgtc cctgatggtc gtcatctacc
     9781 tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc
     9841 ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg
     9901 tcggccccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct
     9961 gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg
    10021 gagtggtgaa tccgttagcg aggtgccgcc ctgcttcatc cccgtggccc gttgctcgcg
    10081 tttgctggcg gtgtccccgg aagaaatata tttgcatgtc tttagttcta tgatgacaca
    10141 aaccccgccc agcgtcttgt cattggcgaa ttcgaacacg cagatgcagt cggggcggcg
    10201 cggtccgagg tccacttcgc atattaaggt gacgcgtgtg gcctcgaaca ccgagcgacc
    10261 ctgcagcgac ccgcttaaca gcgtcaacag cgtgccgcag atcccggggg gcaatgagat
    10321 atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac
    10381 agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat
    10441 gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat
    10501 cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt
    10561 ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg
    10621 caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat
    10681 gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga
    10741 atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat
    10801 cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag
    10861 ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc
    10921 tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg
    10981 atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct
    11041 tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg
    11101 cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac
    11161 ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga
    11221 gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc
    11281 tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc ggatcgggag
    11341 atgggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac
    11401 ggcaataaaa agacagaata aaacgcacgg gtgttgggtc gtttgttcat aaacgcgggg
    11461 ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg
    11521 cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg
    11581 cagccaacgt cggggcggca ggccctgcca tagccactgg ccccgtgggt tagggacggg
    11641 gtcccccatg gggaatggtt tatggttcgt gggggttatt attttgggcg ttgcgtgggg
    11701 tcaggtccac gactggactg agcagacaga cccatggttt ttggatggcc tgggcatgga
    11761 ccgcatgtac tggcgcgaca cgaacaccgg gcgtctgtgg ctgccaaaca cccccgaccc
    11821 ccaaaaacca ccgcgcggat ttctggcgtg ccaagctagt cgaccaattc tcatgtttga
    11881 cagcttatca tcgcagatcc gggcaacgtt gttgccattg ctgcaggcgc agaactggta
    11941 ggtatggaag atctatacat tgaatcaata ttggcaatta gccatattag tcattggtta
    12001 tatagcataa atcaatattg gctattggcc attgcatacg ttgtatctat atcataatat
    12061 gtacatttat attggctcat gtccaatatg accgccatgt tgacattgat tattgactag
    12121 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
    12181 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
    12241 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
    12301 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
    12361 tccgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
    12421 gaccttacgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
    12481 ggtgatgcgg ttttggcagt acaccaatgg gcgtggatag cggtttgact cacggggatt
    12541 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
    12601 ctttccaaaa tgtcgtaata accccgcccc gttgacgcaa atgggcggta ggcgtgtacg
    12661 gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatctcta gaagctgggt
    12721 accagctgct agc
//
