LOCUS       sequence-235233-       12406 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the SHIV327C-Hu A10 
            strain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12406)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 12406)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-SHIV327C-HuA10-gp160
FEATURES             Location/Qualifiers
     source          1..12406
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    2255..2389
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2279..2298)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2333..2352
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2532..2554)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      3114..4903
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(5205..7130)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(7565..7583)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7651..7755
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7756..7824,7825..8616)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7974..7993)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8787..9375
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9276..9295
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        9806..9951
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             9994..11031
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(11029..11048)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11073..11121
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        11775..12154
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12155..12358
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     12309..12329
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     12355..12379
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gccaccatgc ctatgggttc tttgcagccc ctggccacgc tgtacctgct cggcatgctg
       61 gttgcctcag tattggcgaa cttgtgggtt acagtgtatt acggcgttcc agtgtggaaa
      121 gaggcaaaaa ctacactctt ctgtgcgagc gatgccaaag catatgaaca ggaagttcac
      181 aatgtatggg caactcatgc atgcgtgccg acagacccta acccccaaga gatgcggatg
      241 gaaaatgtta cggaaaactt caacatgtgg aagaatgata tggtcgacca gatgcacgag
      301 gatataatta gcttgtggga tcagtccctt aagccctgcg tgaaacttac cccactttgc
      361 gttacattgg attgccaaaa tgtcaatgcc actcaaaata ccaatgacac aatatccacc
      421 atgaaaaatt gcaccttcaa cacaacagca gatttgggag ataaaaagca gaaagggaga
      481 gcgctctttt acaacttgga catcgtccaa ctcaatccta attctaattc atctgagtac
      541 aggctgatct cctgcaatac atccacaatc acccaggcct gccccaaggt ctcttttgaa
      601 cccatcccca tacactactg cgcgcccgcc gggtatgcta ttcttaaatg caacaacaag
      661 acctttaatg gcctcggccc ttgcacaaac gtcagcacag tacagtgtac ccatggcata
      721 aaaccagtgg tctctacaca attgctcctc aacggttctc ttgctgaggg agaaattatc
      781 ataaggtccg agaatttgac cgataacggt aaaacaatta tcgttcatct taacgaaagc
      841 gtgaaaatag tgtgtattcg cccaaacaat aacacccgga agagcatcag gatagggccg
      901 ggacagacat tctttgcaac agacatcata ggagacatca ggcaagccta ttgcaacatc
      961 agcagggagg actggaataa aacactggac agagtacgaa aaaaactgga ggagcacttc
     1021 cccaacaaaa ctattgaatt taagaggcat tctggcggtg atcttgaagt gacgcagcac
     1081 agtttcaact gtcgcggaga gtttttttat tgtaacacta cgcacctttt tgaaaatacg
     1141 acttatacca acagtagtaa catcactttg ccgtgcagaa ttaagcaaat aatcaatatg
     1201 tggcagggag tcggcagagc tatgtatgcc cctcctatcg ctgggaacat aacctgtata
     1261 tctaatatca ccggtattct cctcacaaga gatgggggga ataacggaac aaatgagact
     1321 tttagaccgg gaggagggga catgcgagac aattggagga gcgaattgta caagtataaa
     1381 gtagtggaaa ttaagccact tggcatagct ccgaccaagg cgaagcggag agttgttgag
     1441 agagagaaaa gagccgtcgg aatgggagcg ctgtttctgg gatttctcgg agctgctggt
     1501 tcaaccatgg gggctgcgtc tatgaccctg acagttcaag ctcggcagtt gttgtccggt
     1561 atcgtccaac aacagagtaa cttgctgcgc gccatagagg cgcaacaaca catgttgcag
     1621 cttacggtct gggggattaa gcaactgcag gcgcgcgtac tcgcactgga gagatacttg
     1681 caagatcagc agctgttggg aatatgggga tgttcaggca aactgatctg tactacagca
     1741 gttccttgga atagttcctg gagcaacaag acatacaatg acatatggga taacatgacg
     1801 tggatgcagt gggaaagaga aatcagcaac tatacgaata cgatatacac cttgctcgaa
     1861 gtatcccaaa atcaacaaga gcaaaacgag aaagatttgc tcgctctgga caagtggcaa
     1921 aacctttgga gttggtttaa cataaccaac tggctgtggt acatcaaaat atttattatg
     1981 atagtaggtg gtttgatagg cctcaggatt atcttcgcag tcctcagcat tattaatagg
     2041 gtgaggcaag gttactctcc attgtctttt cagactctca ccccgaatcc cagaggaccg
     2101 gatcgactcg ggggaataga ggaagaaggt ggggagcaag accgcgatcg aagtattaga
     2161 ctggttagcg ggttcctctc tttggcttgg gacgacctca gaagtctctg tctcttctct
     2221 taccacagat agtaactcga ggccggcaag gccggatcca gacatgataa gatacattga
     2281 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     2341 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     2401 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta
     2461 aaacctctac aaatgtggta tggctgatta tgatccggct gcctcgcgcg tttcggtgat
     2521 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     2581 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     2641 gcagccatga ggtcgactct agaggatcga tccccgcccc ggacgaacta aacctgacta
     2701 cgacatctct gccccttctt cgcggggcag tgcatgtaat cccttcagtt ggttggtaca
     2761 acttgccaac tgggccctgt tccacatgtg acacgggggg ggaccaaaca caaaggggtt
     2821 ctctgactgt agttgacatc cttataaatg gatgtgcaca tttgccaaca ctgagtggct
     2881 ttcatcctgg agcagacttt gcagtctgtg gactgcaaca caacattgcc tttatgtgta
     2941 actcttggct gaagctctta caccaatgct gggggacatg tacctcccag gggcccagga
     3001 agactacggg aggctacacc aacgtcaatc agaggggcct gtgtagctac cgataagcgg
     3061 accctcaaga gggcattagc aatagtgttt ataaggcccc cttgttaacc ctaaacgggt
     3121 agcatatgct tcccgggtag tagtatatac tatccagact aaccctaatt caatagcata
     3181 tgttacccaa cgggaagcat atgctatcga attagggtta gtaaaagggt cctaaggaac
     3241 agcgatatct cccaccccat gagctgtcac ggttttattt acatggggtc aggattccac
     3301 gagggtagtg aaccatttta gtcacaaggg cagtggctga agatcaagga gcgggcagtg
     3361 aactctcctg aatcttcgcc tgcttcttca ttctccttcg tttagctaat agaataactg
     3421 ctgagttgtg aacagtaagg tgtatgtgag gtgctcgaaa acaaggtttc aggtgacgcc
     3481 cccagaataa aatttggacg gggggttcag tggtggcatt gtgctatgac accaatataa
     3541 ccctcacaaa ccccttgggc aataaatact agtgtaggaa tgaaacattc tgaatatctt
     3601 taacaataga aatccatggg gtggggacaa gccgtaaaga ctggatgtcc atctcacacg
     3661 aatttatggc tatgggcaac acataatcct agtgcaatat gatactgggg ttattaagat
     3721 gtgtcccagg cagggaccaa gacaggtgaa ccatgttgtt acactctatt tgtaacaagg
     3781 ggaaagagag tggacgccga cagcagcgga ctccactggt tgtctctaac acccccgaaa
     3841 attaaacggg gctccacgcc aatggggccc ataaacaaag acaagtggcc actctttttt
     3901 ttgaaattgt ggagtggggg cacgcgtcag cccccacacg ccgccctgcg gttttggact
     3961 gtaaaataag ggtgtaataa cttggctgat tgtaaccccg ctaaccactg cggtcaaacc
     4021 acttgcccac aaaaccacta atggcacccc ggggaatacc tgcataagta ggtgggcggg
     4081 ccaagatagg ggcgcgattg ctgcgatctg gaggacaaat tacacacact tgcgcctgag
     4141 cgccaagcac agggttgttg gtcctcatat tcacgaggtc gctgagagca cggtgggcta
     4201 atgttgccat gggtagcata tactacccaa atatctggat agcatatgct atcctaatct
     4261 atatctgggt agcataggct atcctaatct atatctgggt agcatatgct atcctaatct
     4321 atatctgggt agtatatgct atcctaattt atatctgggt agcataggct atcctaatct
     4381 atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct atcctaatct
     4441 gtatccgggt agcatatgct atcctaatag agattagggt agtatatgct atcctaattt
     4501 atatctgggt agcatatact acccaaatat ctggatagca tatgctatcc taatctatat
     4561 ctgggtagca tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat
     4621 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatttatat
     4681 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     4741 ctgggtagta tatgctatcc taatctgtat ccgggtagca tatgctatcc tcatgcatat
     4801 acagtcagca tatgataccc agtagtagag tgggagtgct atcctttgca tatgccgcca
     4861 cctcccaagg gggcgtgaat tttcgctgct tgtccttttc ctgctggttg ctcccattct
     4921 taggtgaatt taaggaggcc aggctaaagc cgtcgcatgt ctgattgctc accaggtaaa
     4981 tgtcgctaat gttttccaac gcgagaaggt gttgagcgcg gagctgagtg acgtgacaac
     5041 atgggtatgc ccaattgccc catgttggga ggacgaaaat ggtgacaaga cagatggcca
     5101 gaaatacacc aacagcacgc atgatgtcta ctggggattt attctttagt gcgggggaat
     5161 acacggcttt taatacgatt gagggcgtct cctaacaagt tacatcactc ctgcccttcc
     5221 tcaccctcat ctccatcacc tccttcatct ccgtcatctc cgtcatcacc ctccgcggca
     5281 gccccttcca ccataggtgg aaaccaggga ggcaaatcta ctccatcgtc aaagctgcac
     5341 acagtcaccc tgatattgca ggtaggagcg ggctttgtca taacaaggtc cttaatcgca
     5401 tccttcaaaa cctcagcaaa tatatgagtt tgtaaaaaga ccatgaaata acagacaatg
     5461 gactccctta gcgggccagg ttgtgggccg ggtccagggg ccattccaaa ggggagacga
     5521 ctcaatggtg taagacgaca ttgtggaata gcaagggcag ttcctcgcct taggttgtaa
     5581 agggaggtct tactacctcc atatacgaac acaccggcga cccaagttcc ttcgtcggta
     5641 gtcctttcta cgtgactcct agccaggaga gctcttaaac cttctgcaat gttctcaaat
     5701 ttcgggttgg aacctccttg accacgatgc tttccaaacc accctccttt tttgcgcctg
     5761 cctccatcac cctgaccccg gggtccagtg cttgggcctt ctcctgggtc atctgcgggg
     5821 ccctgctcta tcgctcccgg gggcacgtca ggctcaccat ctgggccacc ttcttggtgg
     5881 tattcaaaat aatcggcttc ccctacaggg tggaaaaatg gccttctacc tggagggggc
     5941 ctgcgcggtg gagacccgga tgatgatgac tgactactgg gactcctggg cctcttttct
     6001 ccacgtccac gacctctccc cctggctctt tcacgacttc cccccctggc tctttcacgt
     6061 cctctacccc ggcggcctcc actacctcct cgaccccggc ctccactacc tcctcgaccc
     6121 cggcctccac tgcctcctcg accccggcct ccacctcctg ctcctgcccc tcctgctcct
     6181 gcccctcctc ctgctcctgc ccctcctgcc cctcctgctc ctgcccctcc tgcccctcct
     6241 gctcctgccc ctcctgcccc tcctgctcct gcccctcctg cccctcctcc tgctcctgcc
     6301 cctcctgccc ctcctcctgc tcctgcccct cctgcccctc ctgctcctgc ccctcctgcc
     6361 cctcctgctc ctgcccctcc tgcccctcct gctcctgccc ctcctgctcc tgcccctcct
     6421 gctcctgccc ctcctgctcc tgcccctcct gcccctcctg cccctcctcc tgctcctgcc
     6481 cctcctgctc ctgcccctcc tgcccctcct gcccctcctg ctcctgcccc tcctcctgct
     6541 cctgcccctc ctgcccctcc tgcccctcct cctgctcctg cccctcctgc ccctcctcct
     6601 gctcctgccc ctcctcctgc tcctgcccct cctgcccctc ctgcccctcc tcctgctcct
     6661 gcccctcctg cccctcctcc tgctcctgcc cctcctcctg ctcctgcccc tcctgcccct
     6721 cctgcccctc ctcctgctcc tgcccctcct cctgctcctg cccctcctgc ccctcctgcc
     6781 cctcctgccc ctcctcctgc tcctgcccct cctcctgctc ctgcccctcc tgctcctgcc
     6841 cctcccgctc ctgctcctgc tcctgttcca ccgtgggtcc ctttgcagcc aatgcaactt
     6901 ggacgttttt ggggtctccg gacaccatct ctatgtcttg gccctgatcc tgagccgccc
     6961 ggggctcctg gtcttccgcc tcctcgtcct cgtcctcttc cccgtcctcg tccatggtta
     7021 tcaccccctc ttctttgagg tccactgccg ccggagcctt ctggtccaga tgtgtctccc
     7081 ttctctccta ggccatttcc aggtcctgta cctggcccct cgtcagacat gattcacact
     7141 aaaagagatc aatagacatc tttattagac gacgctcagt gaatacaggg agtgcagact
     7201 cctgccccct ccaacagccc ccccaccctc atccccttca tggtcgctgt cagacagatc
     7261 caggtctgaa aattccccat cctccgaacc atcctcgtcc tcatcaccaa ttactcgcag
     7321 cccggaaaac tcccgctgaa catcctcaag atttgcgtcc tgagcctcaa gccaggcctc
     7381 aaattcctcg tccccctttt tgctggacgg tagggatggg gattctcggg acccctcctc
     7441 ttcctcttca aggtcaccag acagagatgc tactggggca acggaagaaa agctgggtgc
     7501 ggcctgtgag gatcagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga
     7561 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     7621 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     7681 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     7741 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     7801 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     7861 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     7921 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     7981 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
     8041 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     8101 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     8161 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     8221 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     8281 cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa
     8341 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     8401 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     8461 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     8521 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     8581 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     8641 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     8701 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     8761 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     8821 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     8881 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     8941 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     9001 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     9061 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     9121 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     9181 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     9241 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     9301 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     9361 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     9421 ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg
     9481 ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc
     9541 gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccc cgagatgcgc cgcgtgcggc
     9601 tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag
     9661 ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc
     9721 gccctgcttc atccccgtgg cccgttgctc gcgtttgctg gcggtgtccc cggaagaaat
     9781 atatttgcat gtctttagtt ctatgatgac acaaaccccg cccagcgtct tgtcattggc
     9841 gaattcgaac acgcagatgc agtcggggcg gcgcggtccg aggtccactt cgcatattaa
     9901 ggtgacgcgt gtggcctcga acaccgagcg accctgcagc gacccgctta acagcgtcaa
     9961 cagcgtgccg cagatcccgg ggggcaatga gatatgaaaa agcctgaact caccgcgacg
    10021 tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg
    10081 gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg
    10141 gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg
    10201 gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat
    10261 tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc
    10321 gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag
    10381 acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat
    10441 ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc
    10501 gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc
    10561 gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc
    10621 cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc
    10681 gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc
    10741 gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt
    10801 ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg
    10861 cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc
    10921 gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga
    10981 aaccgacgcc ccagcactcg tccggatcgg gagatggggg aggctaactg aaacacggaa
    11041 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca
    11101 cgggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg
    11161 ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc
    11221 accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg
    11281 ccatagccac tggccccgtg ggttagggac ggggtccccc atggggaatg gtttatggtt
    11341 cgtgggggtt attattttgg gcgttgcgtg gggtcaggtc cacgactgga ctgagcagac
    11401 agacccatgg tttttggatg gcctgggcat ggaccgcatg tactggcgcg acacgaacac
    11461 cgggcgtctg tggctgccaa acacccccga cccccaaaaa ccaccgcgcg gatttctggc
    11521 gtgccaagct agtcgaccaa ttctcatgtt tgacagctta tcatcgcaga tccgggcaac
    11581 gttgttgcca ttgctgcagg cgcagaactg gtaggtatgg aagatctata cattgaatca
    11641 atattggcaa ttagccatat tagtcattgg ttatatagca taaatcaata ttggctattg
    11701 gccattgcat acgttgtatc tatatcataa tatgtacatt tatattggct catgtccaat
    11761 atgaccgcca tgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
    11821 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
    11881 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
    11941 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
    12001 cttggcagta catcaagtgt atcatatgcc aagtccgccc cctattgacg tcaatgacgg
    12061 taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc ctacttggca
    12121 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacaccaa
    12181 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
    12241 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta ataaccccgc
    12301 cccgttgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg
    12361 tttagtgaac cgtcagatct ctagaagctg ggtaccagct gctagc
//
