LOCUS       sequence-235234-       12424 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the H031 HIV-1 isolate; 
            QES mutant for enhanced presentation of quaternary epitopes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12424)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 12424)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-H031-QES.i07.c09
FEATURES             Location/Qualifiers
     source          1..12424
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1942..1986
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     polyA_signal    2273..2407
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2297..2316)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2351..2370
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2550..2572)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      3132..4921
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(5223..7148)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(7583..7601)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7669..7773
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7774..7842,7843..8634)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7992..8011)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8805..9393
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9294..9313
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        9824..9969
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             10012..11049
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(11047..11066)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11091..11139
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        11793..12172
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12173..12376
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     12327..12347
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     12373..12397
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gccaccatgc cgatgggatc acttcaacct ttggcaaccc tgtatctcct ggggatgttg
       61 gttgccagcg tcctcgcagc agaggacctc tgggttacag tttattatgg agtgccagtc
      121 tggaaggacg cagacactac cctcttctgc gcttcagagg ctaaagcgta tgatactgag
      181 gcacacaacg tctgggccac ccacgcttgc gtaccgacgg acccaaaccc gcaggagata
      241 gtcctgcaaa acgtgaccga gggctttaat atgtggaaaa acaatatggt agaacagatg
      301 cacgaagaca tcatctcttt gtgggatcaa agcctcaagc cgtgtgttaa gcttacccca
      361 ctttgcgtta cactgaattg cactactgcc aatgcgaaca gtacagctaa cgcaacctca
      421 acaacgtcta ccacgtctac agatatgaag aactgttcat ttaacattac aactgacatg
      481 agggacaaag ttcagaagca acacgcattg ttttattcac tcgacctggt ccctatagat
      541 aattccaata atagttatag acttataagt tgtaacacta gtgtcatcac ccaagcctgt
      601 ccaaaaatca gctttgagcc catccctatc cattactgcg ctccggcggg atacgcaatt
      661 atcaagtgta atgataagaa ctttgccggc ataggaacct gcaagaatgt gagtacggtc
      721 cagtgtactc acggcgtaag gcccaccatg agtacccagc ttctcttgaa cggtggactc
      781 accgagggcg aggtcgtcat caaatctgcc aatttcagcg acaacacaaa gacaattata
      841 gtacagttgt ctgagtcaat cgagataaac tgcacacggc caagcaacaa tacacgaaaa
      901 tcaattacta tagggccagg tcgagcattc tacacgaccg gggacatcat aggggacata
      961 aggcgcgccc attgtaatat atctggcgcg aaatggaaca acaccctccg gcgcatcgca
     1021 gaaaagttga aagtccaata cgaaaacaga acaatcgtgt tcaacgcctc ttcaggcggt
     1081 gatcctgaag ttgtcatgca cacctttaat tgtggtgggg aatggttcta ctgcaatacc
     1141 actcaactgt tcaattcaac cttcaatagt acctgggaaa gagaaacaaa tacgacagaa
     1201 ctcaatacaa acatcacaat tacactcccc tgcagaatca aacagataat caatatgtgg
     1261 caagaggtcg ggaaagccat gtacgcgcct cctatccgag gccaaataag gtgtagtagc
     1321 aacataacgg ggcttcttct cacccgagat ggcagccata ataataacac aacggagaca
     1381 ttcagaccag gtggtggtga catgagagat aattggcgca gtgagctgta caaatataag
     1441 gttgttaaaa tagagcctct cggtatcgca cctactaagg caaagaggcg agtagtacaa
     1501 cgggagaaaa gggctgtagg acttggggcg gtgtttctgg gatttctcgg ggcggcagga
     1561 tctacaatgg gggctgccag catcacgctg accgtgcagg cgcggcagct gctgtcaggg
     1621 atcgttcagc agcagcgcaa ccttcttaat gcaatcgagg ctcagcagca ccttctgcag
     1681 ttgacagtat gggggataaa acagcttcag gctcgggtgc tggctataga gcggtacttg
     1741 aaagaccagc agctccttgg tatatgggga tgcagcggca agcttatatg tacgacggct
     1801 gtgccctgga acgcctcctg gtccaacaaa aaccttagtc agatttggga caatatgacg
     1861 tggatggagt gggagaagga gatagacaat tacaccgggt tgatttacaa ccttctggag
     1921 atctcacaaa atcagcagga gaaaaatgag caagagcttc ttgagctcga caagtgggcg
     1981 agtctctgga attggtttga catcacgagc tggctctggt atatcaagat cttcatcatc
     2041 ataataggtg gccttatcag cttgagaatc atctgcgcag tcatcaacat agtgaataag
     2101 gtgagacaac gctactctcc actgagtttc cagacgctgc ttccgacccc acgagggccg
     2161 gataggccgg aacgaatcga agaggaggat cgggaccggg atagagatac cagcgtaagg
     2221 ttggtaacgg gcttcctcgc gcttatttag taactcgagg ccggcaaggc cggatccaga
     2281 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     2341 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     2401 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
     2461 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atccggctgc
     2521 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     2581 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     2641 gttggcgggt gtcggggcgc agccatgagg tcgactctag aggatcgatc cccgccccgg
     2701 acgaactaaa cctgactacg acatctctgc cccttcttcg cggggcagtg catgtaatcc
     2761 cttcagttgg ttggtacaac ttgccaactg ggccctgttc cacatgtgac acgggggggg
     2821 accaaacaca aaggggttct ctgactgtag ttgacatcct tataaatgga tgtgcacatt
     2881 tgccaacact gagtggcttt catcctggag cagactttgc agtctgtgga ctgcaacaca
     2941 acattgcctt tatgtgtaac tcttggctga agctcttaca ccaatgctgg gggacatgta
     3001 cctcccaggg gcccaggaag actacgggag gctacaccaa cgtcaatcag aggggcctgt
     3061 gtagctaccg ataagcggac cctcaagagg gcattagcaa tagtgtttat aaggccccct
     3121 tgttaaccct aaacgggtag catatgcttc ccgggtagta gtatatacta tccagactaa
     3181 ccctaattca atagcatatg ttacccaacg ggaagcatat gctatcgaat tagggttagt
     3241 aaaagggtcc taaggaacag cgatatctcc caccccatga gctgtcacgg ttttatttac
     3301 atggggtcag gattccacga gggtagtgaa ccattttagt cacaagggca gtggctgaag
     3361 atcaaggagc gggcagtgaa ctctcctgaa tcttcgcctg cttcttcatt ctccttcgtt
     3421 tagctaatag aataactgct gagttgtgaa cagtaaggtg tatgtgaggt gctcgaaaac
     3481 aaggtttcag gtgacgcccc cagaataaaa tttggacggg gggttcagtg gtggcattgt
     3541 gctatgacac caatataacc ctcacaaacc ccttgggcaa taaatactag tgtaggaatg
     3601 aaacattctg aatatcttta acaatagaaa tccatggggt ggggacaagc cgtaaagact
     3661 ggatgtccat ctcacacgaa tttatggcta tgggcaacac ataatcctag tgcaatatga
     3721 tactggggtt attaagatgt gtcccaggca gggaccaaga caggtgaacc atgttgttac
     3781 actctatttg taacaagggg aaagagagtg gacgccgaca gcagcggact ccactggttg
     3841 tctctaacac ccccgaaaat taaacggggc tccacgccaa tggggcccat aaacaaagac
     3901 aagtggccac tctttttttt gaaattgtgg agtgggggca cgcgtcagcc cccacacgcc
     3961 gccctgcggt tttggactgt aaaataaggg tgtaataact tggctgattg taaccccgct
     4021 aaccactgcg gtcaaaccac ttgcccacaa aaccactaat ggcaccccgg ggaatacctg
     4081 cataagtagg tgggcgggcc aagatagggg cgcgattgct gcgatctgga ggacaaatta
     4141 cacacacttg cgcctgagcg ccaagcacag ggttgttggt cctcatattc acgaggtcgc
     4201 tgagagcacg gtgggctaat gttgccatgg gtagcatata ctacccaaat atctggatag
     4261 catatgctat cctaatctat atctgggtag cataggctat cctaatctat atctgggtag
     4321 catatgctat cctaatctat atctgggtag tatatgctat cctaatttat atctgggtag
     4381 cataggctat cctaatctat atctgggtag catatgctat cctaatctat atctgggtag
     4441 tatatgctat cctaatctgt atccgggtag catatgctat cctaatagag attagggtag
     4501 tatatgctat cctaatttat atctgggtag catatactac ccaaatatct ggatagcata
     4561 tgctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagcata
     4621 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     4681 tgctatccta atttatatct gggtagcata ggctatccta atctatatct gggtagcata
     4741 tgctatccta atctatatct gggtagtata tgctatccta atctgtatcc gggtagcata
     4801 tgctatcctc atgcatatac agtcagcata tgatacccag tagtagagtg ggagtgctat
     4861 cctttgcata tgccgccacc tcccaagggg gcgtgaattt tcgctgcttg tccttttcct
     4921 gctggttgct cccattctta ggtgaattta aggaggccag gctaaagccg tcgcatgtct
     4981 gattgctcac caggtaaatg tcgctaatgt tttccaacgc gagaaggtgt tgagcgcgga
     5041 gctgagtgac gtgacaacat gggtatgccc aattgcccca tgttgggagg acgaaaatgg
     5101 tgacaagaca gatggccaga aatacaccaa cagcacgcat gatgtctact ggggatttat
     5161 tctttagtgc gggggaatac acggctttta atacgattga gggcgtctcc taacaagtta
     5221 catcactcct gcccttcctc accctcatct ccatcacctc cttcatctcc gtcatctccg
     5281 tcatcaccct ccgcggcagc cccttccacc ataggtggaa accagggagg caaatctact
     5341 ccatcgtcaa agctgcacac agtcaccctg atattgcagg taggagcggg ctttgtcata
     5401 acaaggtcct taatcgcatc cttcaaaacc tcagcaaata tatgagtttg taaaaagacc
     5461 atgaaataac agacaatgga ctcccttagc gggccaggtt gtgggccggg tccaggggcc
     5521 attccaaagg ggagacgact caatggtgta agacgacatt gtggaatagc aagggcagtt
     5581 cctcgcctta ggttgtaaag ggaggtctta ctacctccat atacgaacac accggcgacc
     5641 caagttcctt cgtcggtagt cctttctacg tgactcctag ccaggagagc tcttaaacct
     5701 tctgcaatgt tctcaaattt cgggttggaa cctccttgac cacgatgctt tccaaaccac
     5761 cctccttttt tgcgcctgcc tccatcaccc tgaccccggg gtccagtgct tgggccttct
     5821 cctgggtcat ctgcggggcc ctgctctatc gctcccgggg gcacgtcagg ctcaccatct
     5881 gggccacctt cttggtggta ttcaaaataa tcggcttccc ctacagggtg gaaaaatggc
     5941 cttctacctg gagggggcct gcgcggtgga gacccggatg atgatgactg actactggga
     6001 ctcctgggcc tcttttctcc acgtccacga cctctccccc tggctctttc acgacttccc
     6061 cccctggctc tttcacgtcc tctaccccgg cggcctccac tacctcctcg accccggcct
     6121 ccactacctc ctcgaccccg gcctccactg cctcctcgac cccggcctcc acctcctgct
     6181 cctgcccctc ctgctcctgc ccctcctcct gctcctgccc ctcctgcccc tcctgctcct
     6241 gcccctcctg cccctcctgc tcctgcccct cctgcccctc ctgctcctgc ccctcctgcc
     6301 cctcctcctg ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tgcccctcct
     6361 gctcctgccc ctcctgcccc tcctgctcct gcccctcctg cccctcctgc tcctgcccct
     6421 cctgctcctg cccctcctgc tcctgcccct cctgctcctg cccctcctgc ccctcctgcc
     6481 cctcctcctg ctcctgcccc tcctgctcct gcccctcctg cccctcctgc ccctcctgct
     6541 cctgcccctc ctcctgctcc tgcccctcct gcccctcctg cccctcctcc tgctcctgcc
     6601 cctcctgccc ctcctcctgc tcctgcccct cctcctgctc ctgcccctcc tgcccctcct
     6661 gcccctcctc ctgctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctcctgct
     6721 cctgcccctc ctgcccctcc tgcccctcct cctgctcctg cccctcctcc tgctcctgcc
     6781 cctcctgccc ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tcctgctcct
     6841 gcccctcctg ctcctgcccc tcccgctcct gctcctgctc ctgttccacc gtgggtccct
     6901 ttgcagccaa tgcaacttgg acgtttttgg ggtctccgga caccatctct atgtcttggc
     6961 cctgatcctg agccgcccgg ggctcctggt cttccgcctc ctcgtcctcg tcctcttccc
     7021 cgtcctcgtc catggttatc accccctctt ctttgaggtc cactgccgcc ggagccttct
     7081 ggtccagatg tgtctccctt ctctcctagg ccatttccag gtcctgtacc tggcccctcg
     7141 tcagacatga ttcacactaa aagagatcaa tagacatctt tattagacga cgctcagtga
     7201 atacagggag tgcagactcc tgccccctcc aacagccccc ccaccctcat ccccttcatg
     7261 gtcgctgtca gacagatcca ggtctgaaaa ttccccatcc tccgaaccat cctcgtcctc
     7321 atcaccaatt actcgcagcc cggaaaactc ccgctgaaca tcctcaagat ttgcgtcctg
     7381 agcctcaagc caggcctcaa attcctcgtc cccctttttg ctggacggta gggatgggga
     7441 ttctcgggac ccctcctctt cctcttcaag gtcaccagac agagatgcta ctggggcaac
     7501 ggaagaaaag ctgggtgcgg cctgtgagga tcagcttatc gatgataagc tgtcaaacat
     7561 gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     7621 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     7681 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     7741 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     7801 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     7861 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     7921 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     7981 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc
     8041 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     8101 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     8161 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     8221 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     8281 gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc
     8341 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     8401 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     8461 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     8521 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     8581 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     8641 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     8701 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     8761 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     8821 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     8881 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     8941 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     9001 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     9061 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     9121 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     9181 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     9241 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     9301 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     9361 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     9421 ttcctggcct tttgctggcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac
     9481 agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg
     9541 aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccccg
     9601 agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt
     9661 tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga
     9721 atccgttagc gaggtgccgc cctgcttcat ccccgtggcc cgttgctcgc gtttgctggc
     9781 ggtgtccccg gaagaaatat atttgcatgt ctttagttct atgatgacac aaaccccgcc
     9841 cagcgtcttg tcattggcga attcgaacac gcagatgcag tcggggcggc gcggtccgag
     9901 gtccacttcg catattaagg tgacgcgtgt ggcctcgaac accgagcgac cctgcagcga
     9961 cccgcttaac agcgtcaaca gcgtgccgca gatcccgggg ggcaatgaga tatgaaaaag
    10021 cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc
    10081 gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg
    10141 cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt
    10201 tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc
    10261 agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg
    10321 cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct
    10381 gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa
    10441 tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa
    10501 actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt
    10561 tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat
    10621 gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg
    10681 gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag
    10741 cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg
    10801 gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc
    10861 gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact
    10921 gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa
    10981 gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cggatcggga gatgggggag
    11041 gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa
    11101 aagacagaat aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg gttcggtccc
    11161 agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt
    11221 cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg
    11281 tcggggcggc aggccctgcc atagccactg gccccgtggg ttagggacgg ggtcccccat
    11341 ggggaatggt ttatggttcg tgggggttat tattttgggc gttgcgtggg gtcaggtcca
    11401 cgactggact gagcagacag acccatggtt tttggatggc ctgggcatgg accgcatgta
    11461 ctggcgcgac acgaacaccg ggcgtctgtg gctgccaaac acccccgacc cccaaaaacc
    11521 accgcgcgga tttctggcgt gccaagctag tcgaccaatt ctcatgtttg acagcttatc
    11581 atcgcagatc cgggcaacgt tgttgccatt gctgcaggcg cagaactggt aggtatggaa
    11641 gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt atatagcata
    11701 aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata tgtacattta
    11761 tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta gttattaata
    11821 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
    11881 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
    11941 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
    12001 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtccgccccc
    12061 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttacg
    12121 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
    12181 gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
    12241 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
    12301 atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt
    12361 ctatataagc agagctcgtt tagtgaaccg tcagatctct agaagctggg taccagctgc
    12421 tagc
//
