LOCUS       sequence-235240-       12754 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the BG505 HIV-1 isolate 
            (containing SOSIP mutations); QES mutant for enhanced presentation 
            of quaternary epitopes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12754)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 12754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-BG505.SOSIP-gp160-QES.i05.c06
FEATURES             Location/Qualifiers
     source          1..12754
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    2603..2737
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2627..2646)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2681..2700
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2880..2902)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      3462..5251
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(5553..7478)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(7913..7931)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7999..8103
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8104..8172,8173..8964)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8322..8341)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9135..9723
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9624..9643
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        10154..10299
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             10342..11379
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(11377..11396)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11421..11469
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        12123..12502
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12503..12706
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     12657..12677
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     12703..12727
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gccaccatgc caatgggctc ccttcagccc ttggctaccc tgtaccttct tgggatgttg
       61 gtcgcatctg tgcttgccgc agaaaacttg tgggtaactg tctactatgg agtacccgtt
      121 tggaaagacg cagaaacaac gcttttttgc gcatccgacg cgaaggcata cgaaacggaa
      181 aagcacaatg tttgggcaac gcatgcctgc gtcccgacag acccaaatcc tcaggagatt
      241 caccttgaga atgtaacaga ggaattcaac atgtggaaga ataacatggt agaacaaatg
      301 catacggata ttatcagtct ttgggaccag agccttaaac cttgcgttaa acttacgccc
      361 ctctgcgtaa ctctgcaatg tactaacgta acgaataaca ttacggacga catgcgcggc
      421 gaactgaaga attgcagctt taatatgaca acggagttgc gagataaaaa gcagaaggtg
      481 tacagcctct tctacaggct tgacctcgtg caaataaacg aaaaccaggg gaacaggagc
      541 aataactcca ataaagaata cagattgatc aactgtaaca ctagcgagat aacccaggcc
      601 tgcccgaagg tgtcatttga gccgattcct atacactatt gcgccccggc agggtatgcg
      661 atcttgaaat gcaaggataa gaagtttaat ggaactggtc cctgtcccag tgtgagtacg
      721 gttcagtgta cccacggcat aaagccagtg atgtccactc agcttctcct caatggcagc
      781 ctcgcagagg aagaggtcat gatacggagt gagaatatta ctaacaatgc caagaatatc
      841 ttggtccaat ttaatacccc cgtccagatt aattgtaccc gacctaataa taataccaga
      901 aaatccattc gcatcggacc aggtcaggcg ttctatgcca cgggagatat aataggtgac
      961 atccgacagg cgcattgcaa tgtaagcaag gccacatgga atgagactct gggtaaagtt
     1021 gtcaagcagc tcaggaagca tttcgggaac aataccatca tccgatttgc gaacagtagt
     1081 gggggtgatc ttgaagtaac tacacattca tttaactgcg gcggggagtt tttttattgt
     1141 aacacatccg ggttgttcaa ctccacttgg atatctaaca ctagtgttca gggatcaaac
     1201 tccacaggat caaacgatag tataacactt ccttgtcgca tcaaacagat tatcaacatg
     1261 tggcagcgaa tcggccaggc gatgtacgcc ccgcctatcc agggagtaat acggtgcgta
     1321 agcaacataa cgggccttat acttacgcgc gatggaggca gcactaattc taccactgag
     1381 acgttccggc ctggcggtgg ggacatgcga gacaactggc ggtctgagtt gtggaagtac
     1441 aaagtggtta aaatcgaacc cctgggtgtc gccccaacga gatgtaaacg acgggtagtg
     1501 ggaaggagga gaaggcgacg ggcggtcggc attggggccg ttttcttggg ttttctgggg
     1561 gcggctggga gcactatggg agcagctagt atgaccctga ccgtacaggc gaggaactac
     1621 ctgtccggta tagtgcaaca acagtcaaac cttcttaggg ctcctgaggc gcagcaacat
     1681 ctccttaagt tgactgtctg gggcataaag caactccaag cgcgggttct cgcggtggaa
     1741 agatacctta gggaccaaca acttttgggg atgtggggtt gttcaggcaa actcatctgt
     1801 tgcactaacg ttccttggaa ctcctcatgg tctaaccgaa atctctccga gatctgggat
     1861 aatatgacat ggttgcagtg ggataaggag attagcaatt acactcaaat aatctatggg
     1921 ctcctggagg aaagccagaa tcagcaggag aagaatgaac aggatcttct ggcccttgat
     1981 aagtgggcaa gtctttggaa ttggttcgac atttcaaatt ggctttggta tataaagata
     2041 tttataatga ttgtcggggg tttgataggt ttgaggattg tgtttgctgt gctgtctgtt
     2101 atccacaggg ttaggcaggg gtatagtccg ctctcttttc aaacccatac ccccaacccc
     2161 aggggattgg accgacccga gcggattgag gaggaagacg gtgaacagga ccggggcaga
     2221 agtacccgcc ttgttagcgg gtttctggct ctggcgtggg acgacttgcg gagcctttgc
     2281 ctcttctgct accatcgcct cagagatttt atcttgatcg ctgccagaat agtagaattg
     2341 ttgggccaca gctccctcaa aggtttgaga cttggatggg aaggtcttaa atacctgtgg
     2401 aatttgctgg cttactgggg tagggagctg aagatttctg ccattaactt gttcgacaca
     2461 atagctattg cagttgcgga gtggactgac agagtcatag aaataggcca aagattgtgt
     2521 cgggcgtttc tccacattcc acggcggatt aggcaaggac ttgaacgggc cctcctttag
     2581 taactcgagg ccggcaaggc cggatccaga catgataaga tacattgatg agtttggaca
     2641 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     2701 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     2761 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa
     2821 atgtggtatg gctgattatg atccggctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     2881 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     2941 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgagg
     3001 tcgactctag aggatcgatc cccgccccgg acgaactaaa cctgactacg acatctctgc
     3061 cccttcttcg cggggcagtg catgtaatcc cttcagttgg ttggtacaac ttgccaactg
     3121 ggccctgttc cacatgtgac acgggggggg accaaacaca aaggggttct ctgactgtag
     3181 ttgacatcct tataaatgga tgtgcacatt tgccaacact gagtggcttt catcctggag
     3241 cagactttgc agtctgtgga ctgcaacaca acattgcctt tatgtgtaac tcttggctga
     3301 agctcttaca ccaatgctgg gggacatgta cctcccaggg gcccaggaag actacgggag
     3361 gctacaccaa cgtcaatcag aggggcctgt gtagctaccg ataagcggac cctcaagagg
     3421 gcattagcaa tagtgtttat aaggccccct tgttaaccct aaacgggtag catatgcttc
     3481 ccgggtagta gtatatacta tccagactaa ccctaattca atagcatatg ttacccaacg
     3541 ggaagcatat gctatcgaat tagggttagt aaaagggtcc taaggaacag cgatatctcc
     3601 caccccatga gctgtcacgg ttttatttac atggggtcag gattccacga gggtagtgaa
     3661 ccattttagt cacaagggca gtggctgaag atcaaggagc gggcagtgaa ctctcctgaa
     3721 tcttcgcctg cttcttcatt ctccttcgtt tagctaatag aataactgct gagttgtgaa
     3781 cagtaaggtg tatgtgaggt gctcgaaaac aaggtttcag gtgacgcccc cagaataaaa
     3841 tttggacggg gggttcagtg gtggcattgt gctatgacac caatataacc ctcacaaacc
     3901 ccttgggcaa taaatactag tgtaggaatg aaacattctg aatatcttta acaatagaaa
     3961 tccatggggt ggggacaagc cgtaaagact ggatgtccat ctcacacgaa tttatggcta
     4021 tgggcaacac ataatcctag tgcaatatga tactggggtt attaagatgt gtcccaggca
     4081 gggaccaaga caggtgaacc atgttgttac actctatttg taacaagggg aaagagagtg
     4141 gacgccgaca gcagcggact ccactggttg tctctaacac ccccgaaaat taaacggggc
     4201 tccacgccaa tggggcccat aaacaaagac aagtggccac tctttttttt gaaattgtgg
     4261 agtgggggca cgcgtcagcc cccacacgcc gccctgcggt tttggactgt aaaataaggg
     4321 tgtaataact tggctgattg taaccccgct aaccactgcg gtcaaaccac ttgcccacaa
     4381 aaccactaat ggcaccccgg ggaatacctg cataagtagg tgggcgggcc aagatagggg
     4441 cgcgattgct gcgatctgga ggacaaatta cacacacttg cgcctgagcg ccaagcacag
     4501 ggttgttggt cctcatattc acgaggtcgc tgagagcacg gtgggctaat gttgccatgg
     4561 gtagcatata ctacccaaat atctggatag catatgctat cctaatctat atctgggtag
     4621 cataggctat cctaatctat atctgggtag catatgctat cctaatctat atctgggtag
     4681 tatatgctat cctaatttat atctgggtag cataggctat cctaatctat atctgggtag
     4741 catatgctat cctaatctat atctgggtag tatatgctat cctaatctgt atccgggtag
     4801 catatgctat cctaatagag attagggtag tatatgctat cctaatttat atctgggtag
     4861 catatactac ccaaatatct ggatagcata tgctatccta atctatatct gggtagcata
     4921 tgctatccta atctatatct gggtagcata ggctatccta atctatatct gggtagcata
     4981 tgctatccta atctatatct gggtagtata tgctatccta atttatatct gggtagcata
     5041 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     5101 tgctatccta atctgtatcc gggtagcata tgctatcctc atgcatatac agtcagcata
     5161 tgatacccag tagtagagtg ggagtgctat cctttgcata tgccgccacc tcccaagggg
     5221 gcgtgaattt tcgctgcttg tccttttcct gctggttgct cccattctta ggtgaattta
     5281 aggaggccag gctaaagccg tcgcatgtct gattgctcac caggtaaatg tcgctaatgt
     5341 tttccaacgc gagaaggtgt tgagcgcgga gctgagtgac gtgacaacat gggtatgccc
     5401 aattgcccca tgttgggagg acgaaaatgg tgacaagaca gatggccaga aatacaccaa
     5461 cagcacgcat gatgtctact ggggatttat tctttagtgc gggggaatac acggctttta
     5521 atacgattga gggcgtctcc taacaagtta catcactcct gcccttcctc accctcatct
     5581 ccatcacctc cttcatctcc gtcatctccg tcatcaccct ccgcggcagc cccttccacc
     5641 ataggtggaa accagggagg caaatctact ccatcgtcaa agctgcacac agtcaccctg
     5701 atattgcagg taggagcggg ctttgtcata acaaggtcct taatcgcatc cttcaaaacc
     5761 tcagcaaata tatgagtttg taaaaagacc atgaaataac agacaatgga ctcccttagc
     5821 gggccaggtt gtgggccggg tccaggggcc attccaaagg ggagacgact caatggtgta
     5881 agacgacatt gtggaatagc aagggcagtt cctcgcctta ggttgtaaag ggaggtctta
     5941 ctacctccat atacgaacac accggcgacc caagttcctt cgtcggtagt cctttctacg
     6001 tgactcctag ccaggagagc tcttaaacct tctgcaatgt tctcaaattt cgggttggaa
     6061 cctccttgac cacgatgctt tccaaaccac cctccttttt tgcgcctgcc tccatcaccc
     6121 tgaccccggg gtccagtgct tgggccttct cctgggtcat ctgcggggcc ctgctctatc
     6181 gctcccgggg gcacgtcagg ctcaccatct gggccacctt cttggtggta ttcaaaataa
     6241 tcggcttccc ctacagggtg gaaaaatggc cttctacctg gagggggcct gcgcggtgga
     6301 gacccggatg atgatgactg actactggga ctcctgggcc tcttttctcc acgtccacga
     6361 cctctccccc tggctctttc acgacttccc cccctggctc tttcacgtcc tctaccccgg
     6421 cggcctccac tacctcctcg accccggcct ccactacctc ctcgaccccg gcctccactg
     6481 cctcctcgac cccggcctcc acctcctgct cctgcccctc ctgctcctgc ccctcctcct
     6541 gctcctgccc ctcctgcccc tcctgctcct gcccctcctg cccctcctgc tcctgcccct
     6601 cctgcccctc ctgctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctgcccct
     6661 cctcctgctc ctgcccctcc tgcccctcct gctcctgccc ctcctgcccc tcctgctcct
     6721 gcccctcctg cccctcctgc tcctgcccct cctgctcctg cccctcctgc tcctgcccct
     6781 cctgctcctg cccctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctgctcct
     6841 gcccctcctg cccctcctgc ccctcctgct cctgcccctc ctcctgctcc tgcccctcct
     6901 gcccctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctcctgc tcctgcccct
     6961 cctcctgctc ctgcccctcc tgcccctcct gcccctcctc ctgctcctgc ccctcctgcc
     7021 cctcctcctg ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc tgcccctcct
     7081 cctgctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctgcccc tcctgcccct
     7141 cctcctgctc ctgcccctcc tcctgctcct gcccctcctg ctcctgcccc tcccgctcct
     7201 gctcctgctc ctgttccacc gtgggtccct ttgcagccaa tgcaacttgg acgtttttgg
     7261 ggtctccgga caccatctct atgtcttggc cctgatcctg agccgcccgg ggctcctggt
     7321 cttccgcctc ctcgtcctcg tcctcttccc cgtcctcgtc catggttatc accccctctt
     7381 ctttgaggtc cactgccgcc ggagccttct ggtccagatg tgtctccctt ctctcctagg
     7441 ccatttccag gtcctgtacc tggcccctcg tcagacatga ttcacactaa aagagatcaa
     7501 tagacatctt tattagacga cgctcagtga atacagggag tgcagactcc tgccccctcc
     7561 aacagccccc ccaccctcat ccccttcatg gtcgctgtca gacagatcca ggtctgaaaa
     7621 ttccccatcc tccgaaccat cctcgtcctc atcaccaatt actcgcagcc cggaaaactc
     7681 ccgctgaaca tcctcaagat ttgcgtcctg agcctcaagc caggcctcaa attcctcgtc
     7741 cccctttttg ctggacggta gggatgggga ttctcgggac ccctcctctt cctcttcaag
     7801 gtcaccagac agagatgcta ctggggcaac ggaagaaaag ctgggtgcgg cctgtgagga
     7861 tcagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg
     7921 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     7981 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     8041 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     8101 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     8161 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     8221 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     8281 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     8341 tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     8401 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     8461 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     8521 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     8581 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     8641 atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     8701 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     8761 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     8821 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     8881 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     8941 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     9001 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     9061 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     9121 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     9181 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     9241 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     9301 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     9361 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     9421 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     9481 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     9541 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     9601 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     9661 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     9721 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttgaagctgt
     9781 ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg
     9841 ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc
     9901 agcaagacgt agcccagcgc gtcggccccg agatgcgccg cgtgcggctg ctggagatgg
     9961 cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga
    10021 attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cctgcttcat
    10081 ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg gaagaaatat atttgcatgt
    10141 ctttagttct atgatgacac aaaccccgcc cagcgtcttg tcattggcga attcgaacac
    10201 gcagatgcag tcggggcggc gcggtccgag gtccacttcg catattaagg tgacgcgtgt
    10261 ggcctcgaac accgagcgac cctgcagcga cccgcttaac agcgtcaaca gcgtgccgca
    10321 gatcccgggg ggcaatgaga tatgaaaaag cctgaactca ccgcgacgtc tgtcgagaag
    10381 tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa
    10441 tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc
    10501 gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg
    10561 attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg catctcccgc
    10621 cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctgcag
    10681 ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc
    10741 ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg
    10801 attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc
    10861 gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac
    10921 ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg
    10981 gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc
    11041 ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat
    11101 ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa
    11161 ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc
    11221 gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc
    11281 gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc
    11341 agcactcgtc cggatcggga gatgggggag gctaactgaa acacggaagg agacaatacc
    11401 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg ggtgttgggt
    11461 cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga
    11521 gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt
    11581 cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc atagccactg
    11641 gccccgtggg ttagggacgg ggtcccccat ggggaatggt ttatggttcg tgggggttat
    11701 tattttgggc gttgcgtggg gtcaggtcca cgactggact gagcagacag acccatggtt
    11761 tttggatggc ctgggcatgg accgcatgta ctggcgcgac acgaacaccg ggcgtctgtg
    11821 gctgccaaac acccccgacc cccaaaaacc accgcgcgga tttctggcgt gccaagctag
    11881 tcgaccaatt ctcatgtttg acagcttatc atcgcagatc cgggcaacgt tgttgccatt
    11941 gctgcaggcg cagaactggt aggtatggaa gatctataca ttgaatcaat attggcaatt
    12001 agccatatta gtcattggtt atatagcata aatcaatatt ggctattggc cattgcatac
    12061 gttgtatcta tatcataata tgtacattta tattggctca tgtccaatat gaccgccatg
    12121 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
    12181 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
    12241 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
    12301 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
    12361 tcaagtgtat catatgccaa gtccgccccc tattgacgtc aatgacggta aatggcccgc
    12421 ctggcattat gcccagtaca tgaccttacg ggactttcct acttggcagt acatctacgt
    12481 attagtcatc gctattacca tggtgatgcg gttttggcag tacaccaatg ggcgtggata
    12541 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
    12601 ttggcaccaa aatcaacggg actttccaaa atgtcgtaat aaccccgccc cgttgacgca
    12661 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg
    12721 tcagatctct agaagctggg taccagctgc tagc
//
