LOCUS       sequence-235246-       12782 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the CH115 HIV-1 isolate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12782)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 12782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-CH115gp160
FEATURES             Location/Qualifiers
     source          1..12782
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    2627..2761
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2651..2670)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2705..2724
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2904..2926)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      3486..5275
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(5581..7506)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(7941..7959)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8027..8131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8132..8200,8201..8992)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8350..8369)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9163..9751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9652..9671
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        10182..10327
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             10370..11407
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(11405..11424)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11449..11497
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        12151..12530
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12531..12734
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     12685..12705
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     12731..12755
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gccaccatgc cgatgggctc ccttcaaccg ctcgctacgc tgtatcttct cggcatgttg
       61 gtcgctagtg tgctggctgt cggtaatctt tgggttacag tgtactatgg tgtaccagtg
      121 tggagagagg caaccactac tcttttctgt gcttccgacg ccaaagctta tgacgcggaa
      181 gttcacaatg tttgggcaac gcatgcctgc gtacctacag accccaaccc cctcgaaatt
      241 gatctcaaaa atgttactga gaactttaat atgtgggaaa ataagatggt ggatcaaatg
      301 catgaagatg taatcagtct ttgggaccag agcttgaagc catgcgtgaa actgacgccc
      361 ttgtgtgtga cattggaatg ctctaatgta accttggaac ggtcaaatgt ttcaagtagt
      421 aatgacaccc agaatagaac gcaccatgag tccatcaaag agatgaaaaa ttgcagcttc
      481 aacgctacca ccgagctccg agataaaaag caaaaggtgc acgcactctt ttatcgcttg
      541 gacatcgtac cgttggacga gaagaaaaat agcagcgaaa attcttccga aagttacagg
      601 ctcatcaatt gtaataccag tgcaataaca caggcgtgtc caaaggtcac tttcgatccg
      661 atcccaatcc actactgcac tccagcagga tatgcgatac tcaagtgtaa ggacaagatg
      721 ttcaatggaa ccggaccttg ccataatgtg tcaacagtcc agtgtacgca cggaattaag
      781 ccagtggtat ctacgcagct tctcttgaac ggtagcctcg ccgaggggga gataataatt
      841 cgatcagaga acttgacaga caatgcaaag ataatcatag tacatctgaa tcaatctgta
      901 gaaatcaact gcacacggcc ctccaataac actagacaga gtatcagaat cgggccgggg
      961 caaacctttt acgccactgg agagatcatc ggcgacatta gacaggcgca ctgtaacatc
     1021 agcagggcga ggtggaatga aacccttcaa aaggtgggta aaaaactcgc tgagcacttt
     1081 cagaataaga caatcaagtt taactcatct tcaggaggag atctcgaaat tacaactcac
     1141 agtttcaatt gcagaggcga gtttttctat tgcaacactt caggtctctt caacggcaca
     1201 tacatgccca acggcacttt tatcggggcc gaaggcaatt caactataac aattccttgc
     1261 cgaataaagc aaatcattaa tatgtggcaa gaagtaggca gagccatgta cgcccctcca
     1321 atcgctggca atataacctg taatagtaat atcactggcc tgctcctcgt cagggacggg
     1381 ggtaatatca ttgggcaaaa taatactgag acgttcaggc cgcttggggg taacatgaaa
     1441 gataattgga gaagtgaact ttataaatat aaggtagtgg aaataaagcc cttgggcata
     1501 gctcccaccg acgcgaagcg gagggtcgta gaacgagaga agcgcgcggt gggtttgggt
     1561 gccgtatttc tgggattcct tggagccgct ggatccacaa tgggggcggc gtctctcacg
     1621 ctcacagttc aagcgaggca acttctgagt ggaatcgtac agcaacagaa caacctgctt
     1681 agagcgatcg aagctcagca gcatttgctt caattgactg tatgggggat caagcagctg
     1741 caggccaggg tgcttgccat tgagcggtac ttgcaagacc agcaattgct ggggatctgg
     1801 ggttgtagcg gaaaacttat ttgcacgacc gccgtaccgt ggaacgattc atggagcaac
     1861 aagtccttga cagacatctg gaacggcacg acatggatgc aatgggataa ggagattagc
     1921 acatacaccg acatcatata ttccctgctt gaagactcac agattcaaca ggaaaagaat
     1981 gagaaggacc tgttggctct ggactcatgg aaaaatttgt ggaattggtt ctccatcaca
     2041 aactggctgt ggtatattaa gatattcata atgattgtag gggggcttat cggcttgcgc
     2101 attatttttg ctgtgctctc catagtgaat cgggtaaggc aaggatactc cccgctctcc
     2161 ttccagacac ttatacctaa tagcggcggt cctgaccggt tgggtcgcat tgaagaagaa
     2221 ggcggcgaac aggacaaaga cagatccatc cgcctcgtat ctggattcct cgcattggcg
     2281 tgggacgatc tccggtccct ctgtctgttt agttatcacc gcctgagaga tttcattctc
     2341 ctgactgcac gagtggtgga gctgctggga cggaatagcc ttcgagggct ccagcgaggt
     2401 tgggaggccc ttaagtatct gggctccctg gtacagtact gggggcaaga acttaagaag
     2461 agcgccattt cactgttcga taccatagct attgccgtgg cagaaggtac tgatcggatc
     2521 atagagcttg tacaaggctt ttgtagggcg atctataaca ttccgagacg cattcgccag
     2581 ggatttgagg cggcattgca gtagtaactc gaggccggca aggccggatc cagacatgat
     2641 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     2701 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     2761 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
     2821 ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatccgg ctgcctcgcg
     2881 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     2941 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     3001 gggtgtcggg gcgcagccat gaggtcgact ctagaggatc gatccccgcc ccggacgaac
     3061 taaacctgac tacgacatct ctgccccttc ttcgcggggc agtgcatgta atcccttcag
     3121 ttggttggta caacttgcca actgggccct gttccacatg tgacacgggg ggggaccaaa
     3181 cacaaagggg ttctctgact gtagttgaca tccttataaa tggatgtgca catttgccaa
     3241 cactgagtgg ctttcatcct ggagcagact ttgcagtctg tggactgcaa cacaacattg
     3301 cctttatgtg taactcttgg ctgaagctct tacaccaatg ctgggggaca tgtacctccc
     3361 aggggcccag gaagactacg ggaggctaca ccaacgtcaa tcagaggggc ctgtgtagct
     3421 accgataagc ggaccctcaa gagggcatta gcaatagtgt ttataaggcc cccttgttaa
     3481 ccctaaacgg gtagcatatg cttcccgggt agtagtatat actatccaga ctaaccctaa
     3541 ttcaatagca tatgttaccc aacgggaagc atatgctatc gaattagggt tagtaaaagg
     3601 gtcctaagga acagcgatat ctcccacccc atgagctgtc acggttttat ttacatgggg
     3661 tcaggattcc acgagggtag tgaaccattt tagtcacaag ggcagtggct gaagatcaag
     3721 gagcgggcag tgaactctcc tgaatcttcg cctgcttctt cattctcctt cgtttagcta
     3781 atagaataac tgctgagttg tgaacagtaa ggtgtatgtg aggtgctcga aaacaaggtt
     3841 tcaggtgacg cccccagaat aaaatttgga cggggggttc agtggtggca ttgtgctatg
     3901 acaccaatat aaccctcaca aaccccttgg gcaataaata ctagtgtagg aatgaaacat
     3961 tctgaatatc tttaacaata gaaatccatg gggtggggac aagccgtaaa gactggatgt
     4021 ccatctcaca cgaatttatg gctatgggca acacataatc ctagtgcaat atgatactgg
     4081 ggttattaag atgtgtccca ggcagggacc aagacaggtg aaccatgttg ttacactcta
     4141 tttgtaacaa ggggaaagag agtggacgcc gacagcagcg gactccactg gttgtctcta
     4201 acacccccga aaattaaacg gggctccacg ccaatggggc ccataaacaa agacaagtgg
     4261 ccactctttt ttttgaaatt gtggagtggg ggcacgcgtc agcccccaca cgccgccctg
     4321 cggttttgga ctgtaaaata agggtgtaat aacttggctg attgtaaccc cgctaaccac
     4381 tgcggtcaaa ccacttgccc acaaaaccac taatggcacc ccggggaata cctgcataag
     4441 taggtgggcg ggccaagata ggggcgcgat tgctgcgatc tggaggacaa attacacaca
     4501 cttgcgcctg agcgccaagc acagggttgt tggtcctcat attcacgagg tcgctgagag
     4561 cacggtgggc taatgttgcc atgggtagca tatactaccc aaatatctgg atagcatatg
     4621 ctatcctaat ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg
     4681 ctatcctaat ctatatctgg gtagtatatg ctatcctaat ttatatctgg gtagcatagg
     4741 ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg
     4801 ctatcctaat ctgtatccgg gtagcatatg ctatcctaat agagattagg gtagtatatg
     4861 ctatcctaat ttatatctgg gtagcatata ctacccaaat atctggatag catatgctat
     4921 cctaatctat atctgggtag catatgctat cctaatctat atctgggtag cataggctat
     4981 cctaatctat atctgggtag catatgctat cctaatctat atctgggtag tatatgctat
     5041 cctaatttat atctgggtag cataggctat cctaatctat atctgggtag catatgctat
     5101 cctaatctat atctgggtag tatatgctat cctaatctgt atccgggtag catatgctat
     5161 cctcatgcat atacagtcag catatgatac ccagtagtag agtgggagtg ctatcctttg
     5221 catatgccgc cacctcccaa gggggcgtga attttcgctg cttgtccttt tcctgcatgc
     5281 tggttgctcc cattcttagg tgaatttaag gaggccaggc taaagccgtc gcatgtctga
     5341 ttgctcacca ggtaaatgtc gctaatgttt tccaacgcga gaaggtgttg agcgcggagc
     5401 tgagtgacgt gacaacatgg gtatgcccaa ttgccccatg ttgggaggac gaaaatggtg
     5461 acaagacaga tggccagaaa tacaccaaca gcacgcatga tgtctactgg ggatttattc
     5521 tttagtgcgg gggaatacac ggcttttaat acgattgagg gcgtctccta acaagttaca
     5581 tcactcctgc ccttcctcac cctcatctcc atcacctcct tcatctccgt catctccgtc
     5641 atcaccctcc gcggcagccc cttccaccat aggtggaaac cagggaggca aatctactcc
     5701 atcgtcaaag ctgcacacag tcaccctgat attgcaggta ggagcgggct ttgtcataac
     5761 aaggtcctta atcgcatcct tcaaaacctc agcaaatata tgagtttgta aaaagaccat
     5821 gaaataacag acaatggact cccttagcgg gccaggttgt gggccgggtc caggggccat
     5881 tccaaagggg agacgactca atggtgtaag acgacattgt ggaatagcaa gggcagttcc
     5941 tcgccttagg ttgtaaaggg aggtcttact acctccatat acgaacacac cggcgaccca
     6001 agttccttcg tcggtagtcc tttctacgtg actcctagcc aggagagctc ttaaaccttc
     6061 tgcaatgttc tcaaatttcg ggttggaacc tccttgacca cgatgctttc caaaccaccc
     6121 tccttttttg cgcctgcctc catcaccctg accccggggt ccagtgcttg ggccttctcc
     6181 tgggtcatct gcggggccct gctctatcgc tcccgggggc acgtcaggct caccatctgg
     6241 gccaccttct tggtggtatt caaaataatc ggcttcccct acagggtgga aaaatggcct
     6301 tctacctgga gggggcctgc gcggtggaga cccggatgat gatgactgac tactgggact
     6361 cctgggcctc ttttctccac gtccacgacc tctccccctg gctctttcac gacttccccc
     6421 cctggctctt tcacgtcctc taccccggcg gcctccacta cctcctcgac cccggcctcc
     6481 actacctcct cgaccccggc ctccactgcc tcctcgaccc cggcctccac ctcctgctcc
     6541 tgcccctcct gctcctgccc ctcctcctgc tcctgcccct cctgcccctc ctgctcctgc
     6601 ccctcctgcc cctcctgctc ctgcccctcc tgcccctcct gctcctgccc ctcctgcccc
     6661 tcctcctgct cctgcccctc ctgcccctcc tcctgctcct gcccctcctg cccctcctgc
     6721 tcctgcccct cctgcccctc ctgctcctgc ccctcctgcc cctcctgctc ctgcccctcc
     6781 tgctcctgcc cctcctgctc ctgcccctcc tgctcctgcc cctcctgccc ctcctgcccc
     6841 tcctcctgct cctgcccctc ctgctcctgc ccctcctgcc cctcctgccc ctcctgctcc
     6901 tgcccctcct cctgctcctg cccctcctgc ccctcctgcc cctcctcctg ctcctgcccc
     6961 tcctgcccct cctcctgctc ctgcccctcc tcctgctcct gcccctcctg cccctcctgc
     7021 ccctcctcct gctcctgccc ctcctgcccc tcctcctgct cctgcccctc ctcctgctcc
     7081 tgcccctcct gcccctcctg cccctcctcc tgctcctgcc cctcctcctg ctcctgcccc
     7141 tcctgcccct cctgcccctc ctgcccctcc tcctgctcct gcccctcctc ctgctcctgc
     7201 ccctcctgct cctgcccctc ccgctcctgc tcctgctcct gttccaccgt gggtcccttt
     7261 gcagccaatg caacttggac gtttttgggg tctccggaca ccatctctat gtcttggccc
     7321 tgatcctgag ccgcccgggg ctcctggtct tccgcctcct cgtcctcgtc ctcttccccg
     7381 tcctcgtcca tggttatcac cccctcttct ttgaggtcca ctgccgccgg agccttctgg
     7441 tccagatgtg tctcccttct ctcctaggcc atttccaggt cctgtacctg gcccctcgtc
     7501 agacatgatt cacactaaaa gagatcaata gacatcttta ttagacgacg ctcagtgaat
     7561 acagggagtg cagactcctg ccccctccaa cagccccccc accctcatcc ccttcatggt
     7621 cgctgtcaga cagatccagg tctgaaaatt ccccatcctc cgaaccatcc tcgtcctcat
     7681 caccaattac tcgcagcccg gaaaactccc gctgaacatc ctcaagattt gcgtcctgag
     7741 cctcaagcca ggcctcaaat tcctcgtccc cctttttgct ggacggtagg gatggggatt
     7801 ctcgggaccc ctcctcttcc tcttcaaggt caccagacag agatgctact ggggcaacgg
     7861 aagaaaagct gggtgcggcc tgtgaggatc agcttatcga tgataagctg tcaaacatga
     7921 gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     7981 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     8041 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     8101 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     8161 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     8221 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     8281 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     8341 taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg
     8401 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     8461 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     8521 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     8581 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     8641 cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa
     8701 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     8761 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     8821 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     8881 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     8941 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     9001 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     9061 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     9121 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     9181 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     9241 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     9301 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     9361 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     9421 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     9481 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     9541 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     9601 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     9661 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     9721 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     9781 cctggccttt tgctggcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag
     9841 catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa
     9901 ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccccgag
     9961 atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg
    10021 gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat
    10081 ccgttagcga ggtgccgccc tgcttcatcc ccgtggcccg ttgctcgcgt ttgctggcgg
    10141 tgtccccgga agaaatatat ttgcatgtct ttagttctat gatgacacaa accccgccca
    10201 gcgtcttgtc attggcgaat tcgaacacgc agatgcagtc ggggcggcgc ggtccgaggt
    10261 ccacttcgca tattaaggtg acgcgtgtgg cctcgaacac cgagcgaccc tgcagcgacc
    10321 cgcttaacag cgtcaacagc gtgccgcaga tcccgggggg caatgagata tgaaaaagcc
    10381 tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga
    10441 cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg
    10501 tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta
    10561 tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaattcag
    10621 cgagagcctg acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc
    10681 tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc
    10741 ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata
    10801 cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac
    10861 tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg
    10921 ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt
    10981 cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga
    11041 ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca
    11101 gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc
    11161 gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga
    11221 tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt
    11281 cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt
    11341 actcgccgat agtggaaacc gacgccccag cactcgtccg gatcgggaga tgggggaggc
    11401 taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa
    11461 gacagaataa aacgcacggg tgttgggtcg tttgttcata aacgcggggt tcggtcccag
    11521 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct
    11581 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc
    11641 ggggcggcag gccctgccat agccactggc cccgtgggtt agggacgggg tcccccatgg
    11701 ggaatggttt atggttcgtg ggggttatta ttttgggcgt tgcgtggggt caggtccacg
    11761 actggactga gcagacagac ccatggtttt tggatggcct gggcatggac cgcatgtact
    11821 ggcgcgacac gaacaccggg cgtctgtggc tgccaaacac ccccgacccc caaaaaccac
    11881 cgcgcggatt tctggcgtgc caagctagtc gaccaattct catgtttgac agcttatcat
    11941 cgcagatccg ggcaacgttg ttgccattgc tgcaggcgca gaactggtag gtatggaaga
    12001 tctatacatt gaatcaatat tggcaattag ccatattagt cattggttat atagcataaa
    12061 tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata
    12121 ttggctcatg tccaatatga ccgccatgtt gacattgatt attgactagt tattaatagt
    12181 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
    12241 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
    12301 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
    12361 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt ccgcccccta
    12421 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttacggg
    12481 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
    12541 tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
    12601 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
    12661 gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
    12721 atataagcag agctcgttta gtgaaccgtc agatctctag aagctgggta ccagctgcta
    12781 gc
//
