LOCUS       sequence-235248-       12448 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the CH115 HIV-1 isolate; 
            C-terminal truncation; QES mutant for enhanced presentation of 
            quaternary epitopes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12448)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 12448)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-CH115gp160-QES.c14-754*
FEATURES             Location/Qualifiers
     source          1..12448
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    2297..2431
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2321..2340)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2375..2394
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2574..2596)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      3156..4945
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(5247..7172)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(7607..7625)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7693..7797
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7798..7866,7867..8658)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8016..8035)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8829..9417
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9318..9337
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        9848..9993
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             10036..11073
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(11071..11090)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11115..11163
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        11817..12196
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12197..12400
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     12351..12371
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     12397..12421
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gccaccatgc cgatgggctc ccttcaaccg ctcgctacgc tgtatcttct cggcatgttg
       61 gtcgctagtg tgctggctgt cggtaatctt tgggttacag tgtactatgg tgtaccagtg
      121 tggagagagg caaccactac tcttttctgt gcttccgacg ccaaagctta tgacgcggaa
      181 gttcacaatg tttgggcaac gcatgcctgc gtacctacag accccaaccc cctcgaaatt
      241 gatctcaaaa atgttactga gaactttaat atgtgggaaa ataagatggt ggatcaaatg
      301 catgaagatg taatcagtct ttgggaccag agcttgaagc catgcgtgaa actgacgccc
      361 ttgtgtgtga cattggaatg ctctaatgta accttggaac ggtcaaatgt ttcaagtagt
      421 aatgacaccc agaatagaac gcaccatgag tccatcaaag agatgaaaaa ttgcagcttc
      481 aacgctacca ccgagctccg agataaaaag caaaaggtgc acgcactctt ttatcgcttg
      541 gacatcgtac cgttggacga gaagaaaaat agcagcgaaa attcttccga aagttacagg
      601 ctcatcaatt gtaataccag tgcaataaca caggcgtgtc caaagatgac tttcgatccg
      661 atcccaatcc actactgcac tccagcagga tatgcgatac tcaagtgtaa ggacaagatg
      721 ttcaatggaa ccggaccttg ccataatgtg tcaacagtcc agtgtacgca cggaattaag
      781 ccaaccatgt ctacgcagct tctcttgaac ggtagcctcg ccgaggggga gataataatt
      841 cgatcagaga acttgacaga caatgcaaag ccaatcatag tacatctgaa tcaatctgta
      901 gaaatcaact gcacacggcc ctccaataac actagacaga gtatcagaat cgggccgggg
      961 caaacctttt acgccactgg agagatcatc ggcgacatta gacaggcgca ctgtaacatc
     1021 agcagggcga ggtggaatga aacccttcaa aaggtgggta aaaaactcgc tgagcacttt
     1081 cagaataaga caatcaagtt taactcatct tcaggaggag atctcgaaat tacaactcac
     1141 agtttcaatt gcagaggcga gtttttctat tgcaacactt caggtctctt caacggcaca
     1201 tacatgccca acggcacttt tatcggggcc gaaggcaatt caactataac aattccttgc
     1261 cgaataaagc aaatcattaa tatgtggcaa gaagtaggca gagccatgta cgcccctcca
     1321 atcgctggca atataacctg taatagtaat atcactggcc tgctcctcgt cagggacggg
     1381 ggtaatatca ttgggcaaaa taatactgag acgttcaggc cgcttggggg taacatgaaa
     1441 gataattgga gaagtgaact ttataaatat aaggtagtgg aaataaagcc cttgggcata
     1501 gctcccaccg acgcgaagcg gagggtcgta gaacgagaga agcgcgcggt gggtttgggt
     1561 gccgtatttc tgggattcct tggagccgct ggatccacaa tgggggcggc gtctctcacg
     1621 ctcacagttc aagcgaggca acttctgagt ggaatcgtac agcaacagaa caacctgctt
     1681 agagcgatcg aagctcagca gcatttgctt caattgactg tatgggggat caagcagctg
     1741 caggccaggg tgcttgccat tgagcggtac ttgcaagacc agcaattgct ggggatctgg
     1801 ggttgtagcg gaaaacttat ttgcacgacc gccgtaccgt ggaacgattc atggagcaac
     1861 aagtccttga cagacatctg gaacggcacg acatggatgc aatgggataa ggagattagc
     1921 acatacaccg acatcatata ttccctgctt gaagactcac agattcaaca ggaaaagaat
     1981 gagaaggacc tgttggctct ggactcatgg aaaaatttgt ggaattggtt ctccatcaca
     2041 aactggctgt ggtatattaa gatattcata atgattgtag gggggcttat cggcttgcgc
     2101 attatttttg ctgtgctctc catagtgaat cgggtaaggc aaggatactc cccgctctcc
     2161 ttccagacac ttatacctaa tagcggcggt cctgaccggt tgggtcgcat tgaagaagaa
     2221 ggcggcgaac aggacaaaga cagatccatc cgcctcgtat ctggattcct ctaatgactc
     2281 gaggccggca aggccggatc cagacatgat aagatacatt gatgagtttg gacaaaccac
     2341 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     2401 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
     2461 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
     2521 tatggctgat tatgatccgg ctgcctcgcg cgtttcggtg atgacggtga aaacctctga
     2581 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     2641 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gaggtcgact
     2701 ctagaggatc gatccccgcc ccggacgaac taaacctgac tacgacatct ctgccccttc
     2761 ttcgcggggc agtgcatgta atcccttcag ttggttggta caacttgcca actgggccct
     2821 gttccacatg tgacacgggg ggggaccaaa cacaaagggg ttctctgact gtagttgaca
     2881 tccttataaa tggatgtgca catttgccaa cactgagtgg ctttcatcct ggagcagact
     2941 ttgcagtctg tggactgcaa cacaacattg cctttatgtg taactcttgg ctgaagctct
     3001 tacaccaatg ctgggggaca tgtacctccc aggggcccag gaagactacg ggaggctaca
     3061 ccaacgtcaa tcagaggggc ctgtgtagct accgataagc ggaccctcaa gagggcatta
     3121 gcaatagtgt ttataaggcc cccttgttaa ccctaaacgg gtagcatatg cttcccgggt
     3181 agtagtatat actatccaga ctaaccctaa ttcaatagca tatgttaccc aacgggaagc
     3241 atatgctatc gaattagggt tagtaaaagg gtcctaagga acagcgatat ctcccacccc
     3301 atgagctgtc acggttttat ttacatgggg tcaggattcc acgagggtag tgaaccattt
     3361 tagtcacaag ggcagtggct gaagatcaag gagcgggcag tgaactctcc tgaatcttcg
     3421 cctgcttctt cattctcctt cgtttagcta atagaataac tgctgagttg tgaacagtaa
     3481 ggtgtatgtg aggtgctcga aaacaaggtt tcaggtgacg cccccagaat aaaatttgga
     3541 cggggggttc agtggtggca ttgtgctatg acaccaatat aaccctcaca aaccccttgg
     3601 gcaataaata ctagtgtagg aatgaaacat tctgaatatc tttaacaata gaaatccatg
     3661 gggtggggac aagccgtaaa gactggatgt ccatctcaca cgaatttatg gctatgggca
     3721 acacataatc ctagtgcaat atgatactgg ggttattaag atgtgtccca ggcagggacc
     3781 aagacaggtg aaccatgttg ttacactcta tttgtaacaa ggggaaagag agtggacgcc
     3841 gacagcagcg gactccactg gttgtctcta acacccccga aaattaaacg gggctccacg
     3901 ccaatggggc ccataaacaa agacaagtgg ccactctttt ttttgaaatt gtggagtggg
     3961 ggcacgcgtc agcccccaca cgccgccctg cggttttgga ctgtaaaata agggtgtaat
     4021 aacttggctg attgtaaccc cgctaaccac tgcggtcaaa ccacttgccc acaaaaccac
     4081 taatggcacc ccggggaata cctgcataag taggtgggcg ggccaagata ggggcgcgat
     4141 tgctgcgatc tggaggacaa attacacaca cttgcgcctg agcgccaagc acagggttgt
     4201 tggtcctcat attcacgagg tcgctgagag cacggtgggc taatgttgcc atgggtagca
     4261 tatactaccc aaatatctgg atagcatatg ctatcctaat ctatatctgg gtagcatagg
     4321 ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg
     4381 ctatcctaat ttatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg
     4441 ctatcctaat ctatatctgg gtagtatatg ctatcctaat ctgtatccgg gtagcatatg
     4501 ctatcctaat agagattagg gtagtatatg ctatcctaat ttatatctgg gtagcatata
     4561 ctacccaaat atctggatag catatgctat cctaatctat atctgggtag catatgctat
     4621 cctaatctat atctgggtag cataggctat cctaatctat atctgggtag catatgctat
     4681 cctaatctat atctgggtag tatatgctat cctaatttat atctgggtag cataggctat
     4741 cctaatctat atctgggtag catatgctat cctaatctat atctgggtag tatatgctat
     4801 cctaatctgt atccgggtag catatgctat cctcatgcat atacagtcag catatgatac
     4861 ccagtagtag agtgggagtg ctatcctttg catatgccgc cacctcccaa gggggcgtga
     4921 attttcgctg cttgtccttt tcctgctggt tgctcccatt cttaggtgaa tttaaggagg
     4981 ccaggctaaa gccgtcgcat gtctgattgc tcaccaggta aatgtcgcta atgttttcca
     5041 acgcgagaag gtgttgagcg cggagctgag tgacgtgaca acatgggtat gcccaattgc
     5101 cccatgttgg gaggacgaaa atggtgacaa gacagatggc cagaaataca ccaacagcac
     5161 gcatgatgtc tactggggat ttattcttta gtgcggggga atacacggct tttaatacga
     5221 ttgagggcgt ctcctaacaa gttacatcac tcctgccctt cctcaccctc atctccatca
     5281 cctccttcat ctccgtcatc tccgtcatca ccctccgcgg cagccccttc caccataggt
     5341 ggaaaccagg gaggcaaatc tactccatcg tcaaagctgc acacagtcac cctgatattg
     5401 caggtaggag cgggctttgt cataacaagg tccttaatcg catccttcaa aacctcagca
     5461 aatatatgag tttgtaaaaa gaccatgaaa taacagacaa tggactccct tagcgggcca
     5521 ggttgtgggc cgggtccagg ggccattcca aaggggagac gactcaatgg tgtaagacga
     5581 cattgtggaa tagcaagggc agttcctcgc cttaggttgt aaagggaggt cttactacct
     5641 ccatatacga acacaccggc gacccaagtt ccttcgtcgg tagtcctttc tacgtgactc
     5701 ctagccagga gagctcttaa accttctgca atgttctcaa atttcgggtt ggaacctcct
     5761 tgaccacgat gctttccaaa ccaccctcct tttttgcgcc tgcctccatc accctgaccc
     5821 cggggtccag tgcttgggcc ttctcctggg tcatctgcgg ggccctgctc tatcgctccc
     5881 gggggcacgt caggctcacc atctgggcca ccttcttggt ggtattcaaa ataatcggct
     5941 tcccctacag ggtggaaaaa tggccttcta cctggagggg gcctgcgcgg tggagacccg
     6001 gatgatgatg actgactact gggactcctg ggcctctttt ctccacgtcc acgacctctc
     6061 cccctggctc tttcacgact tccccccctg gctctttcac gtcctctacc ccggcggcct
     6121 ccactacctc ctcgaccccg gcctccacta cctcctcgac cccggcctcc actgcctcct
     6181 cgaccccggc ctccacctcc tgctcctgcc cctcctgctc ctgcccctcc tcctgctcct
     6241 gcccctcctg cccctcctgc tcctgcccct cctgcccctc ctgctcctgc ccctcctgcc
     6301 cctcctgctc ctgcccctcc tgcccctcct cctgctcctg cccctcctgc ccctcctcct
     6361 gctcctgccc ctcctgcccc tcctgctcct gcccctcctg cccctcctgc tcctgcccct
     6421 cctgcccctc ctgctcctgc ccctcctgct cctgcccctc ctgctcctgc ccctcctgct
     6481 cctgcccctc ctgcccctcc tgcccctcct cctgctcctg cccctcctgc tcctgcccct
     6541 cctgcccctc ctgcccctcc tgctcctgcc cctcctcctg ctcctgcccc tcctgcccct
     6601 cctgcccctc ctcctgctcc tgcccctcct gcccctcctc ctgctcctgc ccctcctcct
     6661 gctcctgccc ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tgcccctcct
     6721 cctgctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctgcccc tcctcctgct
     6781 cctgcccctc ctcctgctcc tgcccctcct gcccctcctg cccctcctgc ccctcctcct
     6841 gctcctgccc ctcctcctgc tcctgcccct cctgctcctg cccctcccgc tcctgctcct
     6901 gctcctgttc caccgtgggt ccctttgcag ccaatgcaac ttggacgttt ttggggtctc
     6961 cggacaccat ctctatgtct tggccctgat cctgagccgc ccggggctcc tggtcttccg
     7021 cctcctcgtc ctcgtcctct tccccgtcct cgtccatggt tatcaccccc tcttctttga
     7081 ggtccactgc cgccggagcc ttctggtcca gatgtgtctc ccttctctcc taggccattt
     7141 ccaggtcctg tacctggccc ctcgtcagac atgattcaca ctaaaagaga tcaatagaca
     7201 tctttattag acgacgctca gtgaatacag ggagtgcaga ctcctgcccc ctccaacagc
     7261 ccccccaccc tcatcccctt catggtcgct gtcagacaga tccaggtctg aaaattcccc
     7321 atcctccgaa ccatcctcgt cctcatcacc aattactcgc agcccggaaa actcccgctg
     7381 aacatcctca agatttgcgt cctgagcctc aagccaggcc tcaaattcct cgtccccctt
     7441 tttgctggac ggtagggatg gggattctcg ggacccctcc tcttcctctt caaggtcacc
     7501 agacagagat gctactgggg caacggaaga aaagctgggt gcggcctgtg aggatcagct
     7561 tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc
     7621 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     7681 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     7741 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     7801 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     7861 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     7921 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     7981 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     8041 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     8101 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     8161 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     8221 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     8281 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     8341 gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     8401 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     8461 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     8521 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     8581 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     8641 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     8701 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     8761 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     8821 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     8881 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     8941 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     9001 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     9061 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     9121 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     9181 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     9241 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     9301 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     9361 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     9421 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttgaag ctgtccctga
     9481 tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg
     9541 gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag
     9601 acgtagccca gcgcgtcggc cccgagatgc gccgcgtgcg gctgctggag atggcggacg
     9661 cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat
     9721 tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccctgct tcatccccgt
     9781 ggcccgttgc tcgcgtttgc tggcggtgtc cccggaagaa atatatttgc atgtctttag
     9841 ttctatgatg acacaaaccc cgcccagcgt cttgtcattg gcgaattcga acacgcagat
     9901 gcagtcgggg cggcgcggtc cgaggtccac ttcgcatatt aaggtgacgc gtgtggcctc
     9961 gaacaccgag cgaccctgca gcgacccgct taacagcgtc aacagcgtgc cgcagatccc
    10021 ggggggcaat gagatatgaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg
    10081 atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt
    10141 gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat
    10201 ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg
    10261 gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca
    10321 cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc
    10381 gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca
    10441 ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct
    10501 gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg
    10561 caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg
    10621 cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt
    10681 gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg
    10741 aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag
    10801 cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat
    10861 cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca
    10921 atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc
    10981 gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact
    11041 cgtccggatc gggagatggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg
    11101 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacgggtgtt gggtcgtttg
    11161 ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc
    11221 attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg
    11281 aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc actggccccg
    11341 tgggttaggg acggggtccc ccatggggaa tggtttatgg ttcgtggggg ttattatttt
    11401 gggcgttgcg tggggtcagg tccacgactg gactgagcag acagacccat ggtttttgga
    11461 tggcctgggc atggaccgca tgtactggcg cgacacgaac accgggcgtc tgtggctgcc
    11521 aaacaccccc gacccccaaa aaccaccgcg cggatttctg gcgtgccaag ctagtcgacc
    11581 aattctcatg tttgacagct tatcatcgca gatccgggca acgttgttgc cattgctgca
    11641 ggcgcagaac tggtaggtat ggaagatcta tacattgaat caatattggc aattagccat
    11701 attagtcatt ggttatatag cataaatcaa tattggctat tggccattgc atacgttgta
    11761 tctatatcat aatatgtaca tttatattgg ctcatgtcca atatgaccgc catgttgaca
    11821 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
    11881 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
    11941 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
    12001 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
    12061 gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
    12121 ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct acgtattagt
    12181 catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg gatagcggtt
    12241 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
    12301 ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga cgcaaatggg
    12361 cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat
    12421 ctctagaagc tgggtaccag ctgctagc
//
