LOCUS       sequence-235251-        6975 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6975)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 6975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_021220-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..6975
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1086..1105
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1128..1137
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2133..2150
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2202..2257
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2256..2275)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4493..4511)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4579..4683
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4684..4752,4753..5544)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4902..4921)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5715..6303
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6204..6223
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6457..6474
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6591..6612
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6627..6632,6633..6650,6651..6657)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6665..6681
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6670..6692
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6689..6705
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6689..6705
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcctcac tctcttccgc atcgctgtct gcgagggcca gctgttgggc tcgcggttga
      661 ggacaaactc ttcgcggtct ttccagtact cttggatcgg aaacccgtcg gcctccgaac
      721 ggtactccgc caccgaggga cctgagcgag tccgcatcga ccggatcgga aaacctctcg
      781 agaaaggcgt ctaaccagtc acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc
      841 gggtggcggt cggggttgtt tctggcggag gtgctgctga tgatgtaatt aaagtaggcg
      901 gtcttgagac ggcggatggt cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg
      961 tgagtactcc ctctcaaaag cgggcattac ttctgcgcta agattgtcag tttccaaaaa
     1021 cgaggaggat ttgatattca cctggcccga tctggccata cacttgagtg acaatgacat
     1081 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caagtttgcc accatggagt
     1141 ttcagaccca ggtactcatg tccctgctgc tctgcatgtc tggtgcggcc gccgaggaac
     1201 tgtccgtgtc tcctatcgcc caggccctga gagtgcacga gagcggcatc ttcgagtgca
     1261 gcttcgagaa cagcagctac aacctggacc tgcggtggat cctgttcgac ggcaccgtgc
     1321 tgctgaacgg caacaccagc gaggacggcc ggttcatcaa cgaggacggc atcctgcgga
     1381 tgaccaacct gaccatcccc gacagcggcg agtacaactg cgagaacctg gaaatcaaca
     1441 ccaccgtgac cgcccacctg aaggtgtaca tcatgccctc tggcgcgccg tcgacctcca
     1501 tcacggccta taagagtgag ggggagtcag cggagttctc cttcccactc aaccttggag
     1561 aggaaagcct gcagggagag ttgagatgga aggcagagaa ggctccttct tcccagtcct
     1621 ggatcacctt ctccctaaag aaccaaaagg tgtctgtgca gaagtctact agcaacccca
     1681 agttccagct gtccgaaacg ctcccactca cccttcagat accccaggtc tcccttcagt
     1741 ttgctggttc tggcaacctg accctgactc tggacagagg gatactgtat caggaagtga
     1801 acctggtggt gatgaaagtg actcagcccg acagcaacac tttgacctgt gaggtgatgg
     1861 gacccacctc acccaagatg agactgatct tgaagcagga gaatcaggag gccagggtct
     1921 ccaggcagga gaaagtgatt caagtgcagg cccctgaagc aggggtgtgg caatgtctac
     1981 tgagtgaagg tgaagaggtc aagatggact ccaagatcca ggttttatcc aaagggttga
     2041 attccggcag cctgcaccac atcctggacg cccagaagat gctgtggaac cacagggaca
     2101 ggaacctgcc tcctctggcc cctctgggcc cccaccacca tcaccaccat tgagtctaga
     2161 cacgtgatta attaaggatc ccccgacctc gacctctggc taataaagga aatttatttt
     2221 cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc
     2281 aaatcatttg gtcgagatcc ctcggagatc tctagctaga ggatcgatcc ccgccccgga
     2341 cgaactaaac ctgactacga catctctgcc ccttcttcgc ggggcagtgc atgtaatccc
     2401 ttcagttggt tggtacaact tgccaactgg gccctgttcc acatgtgaca cgggggggga
     2461 ccaaacacaa aggggttctc tgactgtagt tgacatcctt ataaatggat gtgcacattt
     2521 gccaacactg agtggctttc atcctggagc agactttgca gtctgtggac tgcaacacaa
     2581 cattgccttt atgtgtaact cttggctgaa gctcttacac caatgctggg ggacatgtac
     2641 ctcccagggg cccaggaaga ctacgggagg ctacaccaac gtcaatcaga ggggcctgtg
     2701 tagctaccga taagcggacc ctcaagaggg cattagcaat agtgtttata aggccccctt
     2761 gttaacccta aacgggtagc atatgcttcc cgggtagtag tatatactat ccagactaac
     2821 cctaattcaa tagcatatgt tacccaacgg gaagcatatg ctatcgaatt agggttagta
     2881 aaagggtcct aaggaacagc gatatctccc accccatgag ctgtcacggt tttatttaca
     2941 tggggtcagg attccacgag ggtagtgaac cattttagtc acaagggcag tggctgaaga
     3001 tcaaggagcg ggcagtgaac tctcctgaat cttcgcctgc ttcttcattc tccttcgttt
     3061 agctaataga ataactgctg agttgtgaac agtaaggtgt atgtgaggtg ctcgaaaaca
     3121 aggtttcagg tgacgccccc agaataaaat ttggacgggg ggttcagtgg tggcattgtg
     3181 ctatgacacc aatataaccc tcacaaaccc cttgggcaat aaatactagt gtaggaatga
     3241 aacattctga atatctttaa caatagaaat ccatggggtg gggacaagcc gtaaagactg
     3301 gatgtccatc tcacacgaat ttatggctat gggcaacaca taatcctagt gcaatatgat
     3361 actggggtta ttaagatgtg tcccaggcag ggaccaagac aggtgaacca tgttgttaca
     3421 ctctatttgt aacaagggga aagagagtgg acgccgacag cagcggactc cactggttgt
     3481 ctctaacacc cccgaaaatt aaacggggct ccacgccaat ggggcccata aacaaagaca
     3541 agtggccact cttttttttg aaattgtgga gtgggggcac gcgtcagccc ccacacgccg
     3601 ccctgcggtt ttggactgta aaataagggt gtaataactt ggctgattgt aaccccgcta
     3661 accactgcgg tcaaaccact tgcccacaaa accactaatg gcaccccggg gaatacctgc
     3721 ataagtaggt gggcgggcca agataggggc gcgattgctg cgatctggag gacaaattac
     3781 acacacttgc gcctgagcgc caagcacagg gttgttggtc ctcatattca cgaggtcgct
     3841 gagagcacgg tgggctaatg ttgccatggg tagcatatac tacccaaata tctggatagc
     3901 atatgctatc ctaatctata tctgggtagc ataggctatc ctaatctata tctgggtagc
     3961 atatgctatc ctaatctata tctgggtagt atatgctatc ctaatttata tctgggtagc
     4021 ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt
     4081 atatgctatc ctaatctgta tccgggtagc atatgctatc ctaatagaga ttagggtagt
     4141 atatgctatc ctaatttata tctgggtagc atatactacc caaatatctg gatagcatat
     4201 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagcatag
     4261 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat
     4321 gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat
     4381 gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg ggtagcatat
     4441 gctatcctca tgataagctg tcaaacatga gaattttctt gaagacgaaa gggcctcgtg
     4501 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     4561 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     4621 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     4681 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     4741 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     4801 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     4861 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     4921 tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     4981 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     5041 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     5101 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     5161 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     5221 atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     5281 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     5341 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     5401 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     5461 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     5521 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     5581 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     5641 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     5701 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     5761 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     5821 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     5881 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     5941 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     6001 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     6061 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     6121 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     6181 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     6241 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     6301 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     6361 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     6421 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     6481 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     6541 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     6601 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     6661 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     6721 ctctagctag aggtcgacca attctcatgt ttgacagctt atcatcgcag atccgggcaa
     6781 cgttgttgcc attgctgcag gcgcagaact ggtaggtatg gaagatctat acattgaatc
     6841 aatattggca attagccata ttagtcattg gttatatagc ataaatcaat attggctatt
     6901 ggccattgca tacgttgtat ctatatcata atatgtacat ttatattggc tcatgtccaa
     6961 tatgaccgcc atgtt
//
