LOCUS       sequence-235262-        5920 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NF-kB p65, kinase sites 276,305 mutated.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5920)
  AUTHORS   He B, Weber GF
  TITLE     Phosphorylation of NF-kappaB proteins by cyclic GMP-dependent 
            kinase. A noncanonical pathway to NF-kappaB activation.
  JOURNAL   Eur J Biochem. 2003 May . 270(10):2174-85.
  PUBMED    12752437
REFERENCE   2  (bases 1 to 5920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eur J 
            Biochem. 2003 May . 270(10):2174-85."
COMMENT     Sequence Label: pRSV-p65T305A,S276A
FEATURES             Location/Qualifiers
     source          1..5920
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..262
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     CDS             1557..1928
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     intron          2022..2087
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2217..2237
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2662..2796
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2699..2718)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2753..2772
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3563..3581)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3649..3753
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3754..3822,3823..4614)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3972..3991)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4785..5373
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5274..5293
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5527..5544
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5625..5644)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 ccgcattgca gagatattgt atttaagtgc ctagctcgat acaataaacg ccatttgacc
      241 attcaccaca ttggtgtgca cctccaagct tcaccatgga cgaactgttc cccctcatct
      301 tcccggcaga gccagcccag gcctctggcc cctatgtgga gatcattgag cagcccaagc
      361 agcggggcat gcgcttccgc tacaagtgcg aggggcgctc cgcgggcagc atcccaggcg
      421 agaggagcac agataccacc aagacccacc ccaccatcaa gatcaatggc tacacaggac
      481 cagggacagt gcgcatctcc ctggtcacca aggaccctcc tcaccggcct cacccccacg
      541 agcttgtagg aaaggactgc cgggatggct tctatgaggc tgagctctgc ccggaccgct
      601 gcatccacag tttccagaac ctgggaatcc agtgtgtgaa gaagcgggac ctggagcagg
      661 ctatcagtca gcgcatccag accaacaaca accccttcca agttcctata gaagagcagc
      721 gtggggacta cgacctgaat gctgtgcggc tctgcttcca ggtgacagtg cgggacccat
      781 caggcaggcc cctccgcctg ccgcctgtcc tttctcatcc catctttgac aatcgtgccc
      841 ccaacactgc cgagctcaag atctgccgag tgaaccgaaa ctctggcagc tgcctcggtg
      901 gggatgagat cttcctactg tgtgacaagg tgcagaaaga ggacattgag gtgtatttca
      961 cgggaccagg ctgggaggcc cgaggctcct tttcgcaagc tgatgtgcac cgacaagtgg
     1021 ccattgtgtt ccggacccct ccctacgcag accccagcct gcaggctcct gtgcgtgtct
     1081 ccatgcagct gcggcggcct gccgaccggg agctcagtga gcccatggaa ttccagtacc
     1141 tgccagatac agacgatcgt caccggattg aggagaaacg taaaagggca tatgagacct
     1201 tcaagagcat catgaagaag agtcctttca gcggacccac cgacccccgg cctccacctc
     1261 gacgcattgc tgtgccttcc cgcagctcag cttctgtccc caagccagca ccccagccct
     1321 atccctttac gtcatccctg agcaccatca actatgatga gtttcccacc atggtgtttc
     1381 cttctgggca gatcagccag gcctcggcct tggccccggc ccctccccaa gtcctgcccc
     1441 aggctccagc ccctgcccct gctccagcca tggtatcagc tctggcccag gccccagccc
     1501 ctgtcccagt cctagcccca ggccctcctc aggctgtggc cccacctgcc cccaagccca
     1561 cccaggctgg ggaaggaacg ctgtcagagg ccctgctgca gctgcagttt gatgatgaag
     1621 acctgggggc cttgcttggc aacagcacag acccagctgt gttcacagac ctggcatccg
     1681 tcgacaactc cgagtttcag cagctgctga accagggcat acctgtggcc ccccacacaa
     1741 ctgagcccat gctgatggag taccctgagg ctataactcg cctagtgaca ggggcccaga
     1801 ggccccccga cccagctcct gctccactgg gggccccggg gctccccaat ggcctccttt
     1861 caggagatga agacttctcc tccattgcgg acatggactt ctcagccctg ctgagtcaga
     1921 tcagctccta agggggtgac ggggggatct ttgtgaagga accttacttc tgtggtgtga
     1981 cataattgga caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa
     2041 gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg
     2101 gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag
     2161 aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa
     2221 aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga
     2281 gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa
     2341 aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt ataagtaggc
     2401 ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat agagtgtctg
     2461 ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta
     2521 ataaggaata tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt
     2581 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     2641 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     2701 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     2761 tccaaactca tcaatgtatc ttatcatgtc tggatcccca ggaagctcct ctgtgtcctc
     2821 ataaacccta acctcctcta cttgagagga cattccaatc ataggctgcc catccaccct
     2881 ctgtgtcctc ctgttaatta ggtcacttaa caaaaaggaa attgggtagg ggtttttcac
     2941 agaccgcttt ctaagggtaa ttttaaaata tctgggaagt cccttccact gctgtgttcc
     3001 agaagtgttg gtaaacagcc cacaaatgtc aacagcagaa acatacaagc tgtcagcttt
     3061 gcacaagggc ccaacaccct gctcatcaag aagcactgtg gttgctgtgt tagtaatgtg
     3121 caaaacagga ggcacatttt ccccacctgt gtaggttcca aaatatctag tgttttcatt
     3181 tttacttgga tcaggaaccc agcactccac tggataagca ttatccttat ccaaaacagc
     3241 cttgtggtca gtgttcatct gctgactgtc aactgtagca ttttttgggg ttacagtttg
     3301 agcaggatat ttggtcctgt agtttgctaa cacaccctgc agctccaaag gttccccacc
     3361 aacagcaaaa aaatgaaaat ttgacccttg aatgggtttt ccagcaccat tttcatgagt
     3421 tttttgtgtc cctgaatgca agtttaacat agcagttacc ccaataacct cagttttaac
     3481 agtaacagct tcccacatca aaatatttcc acaggttaag tcctcattta aattaggcaa
     3541 aggaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     3601 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     3661 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     3721 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     3781 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     3841 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     3901 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     3961 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc
     4021 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     4081 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     4141 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     4201 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     4261 gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc
     4321 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     4381 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     4441 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     4501 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     4561 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     4621 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     4681 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     4741 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     4801 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     4861 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     4921 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     4981 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     5041 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     5101 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     5161 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     5221 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     5281 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     5341 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     5401 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     5461 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     5521 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt
     5581 acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat
     5641 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
     5701 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
     5761 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgtatctga
     5821 ggggactagg gtgtgtttag gcgaaaagcg gggcttcggt tgtacgcggt taggagtccc
     5881 ctcaggatat agtagtttcg cttttgcata gggaggggga
//
