LOCUS       sequence-23527-e         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Ricks DM, Kutner R, Zhang XY, Welsh DA, Reiser J
  TITLE     Optimized lentiviral transduction of mouse bone marrow-derived 
            mesenchymal stem cells.
  JOURNAL   Stem Cells Dev. 2008 Jun . 17(3):441-50.
  PUBMED    18513160
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cells 
            Dev. 2008 Jun . 17(3):441-50."
COMMENT     Sequence Label: pNL-EGFP/CMV/WPREdU3
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    38..626
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(91..111)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(509..520)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ggcaggagag ctgtacagtc cggactcaga tctcgagaat caacctctgg attacaaaat
       61 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
      121 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
      181 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
      241 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
      301 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
      361 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
      421 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
      481 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
      541 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
      601 gatctccctt tgggccgcct ccccgcctgg gtacctttaa gaccaatgac ttacaaggca
      661 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
      721 caaagaagac aagatctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      781 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      841 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      901 agaccctttn agtcagtgtg gaaaaatctc taccacc
//
