LOCUS       sequence-23528-e         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  TITLE     T4 Lysozyme Plasmids - Tray 1
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: T4 Lysozyme I3C
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(707..729)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    718..734
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 gaaaaagccc gctcattagg cgggctcaga tctgctcatg tttgacagct tatcatcgat
       61 aagcttcggc aataaattct tgatatgttt tcatatgttt cctttaaata ttttaattag
      121 taattgtcta ttcaagtaat tgtgaatata ctatcacaat tccaagagaa agtaaacagc
      181 tttatagatt tttatacgcg tcccaagtgc cagttctaaa cgttgtaatg actcgttttg
      241 cgcgattagg tgtttgatat accatctact tttagctaag ttaactgctg cttcatccca
      301 gcgtttttgt tgaagcatac gtaaagagtt agtaaatcct gccacaccgg tttctcccat
      361 ttggaaaacc atattaatca atgcacagcg acgaaccgca tcaagagaat cataaaccgg
      421 ttttaattta gcatttctca gaattccgcg aacagcagca tcaacatcct gattaaagag
      481 tttttcagcc tcatcttttg taattacacc attgcaatta cgcccaatag ctttatctaa
      541 ttcagattta gcagcattaa gtgatggact ttttgtaagc aaatgaccga tgccaatagt
      601 gtaatagcct tctgtgtctt tatagatttt aagtctaaga ccttcatcta tacgtaacat
      661 ttcaaaacaa ttcataatac ctcctaagca tcgatggatc ctgtttcctg tgtgaaattg
      721 ttatccgctc acaattccac acattatacg agccgatgat aattgtcaca gggggatggg
      781 gagtagctga tccggttcng tggaatgtat cgnccattca ccatancgag cgagatatgc
      841 acngggagg
//
