LOCUS       sequence-235287-        3043 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid containing shortened version of V-D CRISPR array for 
            integration assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Wright AV, Wang JY, Burstein D, Harrington LB, Paez-Espino D, 
            Kyrpides NC, Iavarone AT, Banfield JF, Doudna JA
  TITLE     A Functional Mini-Integrase in a Two-Protein-type V-C CRISPR System.
  JOURNAL   Mol Cell. 2019 Feb 21;73(4):727-737.e3. doi: 
            10.1016/j.molcel.2018.12.015. Epub 2019 Jan 29.
  PUBMED    30709710
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2018.12.015"; journalName: "Mol Cell"; date: 
            "2019-02-21- 21"; volume: "73"; issue: "4"; pages: "727-737.e3"
COMMENT     Sequence Label: pVD_CRISPR
FEATURES             Location/Qualifiers
     source          1..3043
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     65..81
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     65..81
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_feature    94..150
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(151..168)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(151..167)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(160..182)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(376..395)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     495..517
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(555..573)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        641..745
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(746..814,815..1606)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(964..983)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1777..2365
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2266..2285
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2519..2536
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcattatttt tactaagcaa ttaagcatac cagacgctct ccatgattat ataacaattt
       61 cacacaggaa acagctatga ccatgattac gccaagcttg catgcctgca ggtcgactct
      121 agaggatccc cgggtaccga gctcgaattc actggccgtc gttttacaac gtcgtgactg
      181 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
      241 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
      301 cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
      361 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
      421 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
      481 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
      541 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
      601 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
      661 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
      721 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
      781 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
      841 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
      901 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
      961 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     1021 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     1081 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     1141 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     1201 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     1261 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     1321 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     1381 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     1441 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     1501 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     1561 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     1621 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     1681 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     1741 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     1801 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     1861 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     1921 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     1981 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     2041 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     2101 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     2161 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     2221 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     2281 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     2341 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     2401 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     2461 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     2521 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcggg ttataccttc
     2581 ttgtaactag gggcaggggt aaagatacgc ggaaacagag tggaacaggc ggagacactc
     2641 ccagtcgtgc tagacttgaa aaacagaata tgccggagtg atgagtggga aggctagttc
     2701 tctttctcgc tctcaacgga gctcaggaga atggaaccat catcattttt ttgcggggat
     2761 acaaaaggaa agagtttcgg gcttgaattg acaagcatgc ctttttccct ctaatcttcg
     2821 gggatcaata ccaaactctg ggcctttttc cctctaatct tcggggagat aaggctagag
     2881 aaaaacctgc tttaccccat aatcagaggt tccccggcat attctatttt caaagaacaa
     2941 tggaatagag cggaaaaaac cctccgtttc tatcccgttt ttgatacgtt tcccaagaaa
     3001 tgcatgtcac tgcgaaccta catcattttc taaaataaga caa
//
