LOCUS       sequence-235289-        3246 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reporter plasmid for full-site selection assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Wright AV, Wang JY, Burstein D, Harrington LB, Paez-Espino D, 
            Kyrpides NC, Iavarone AT, Banfield JF, Doudna JA
  TITLE     A Functional Mini-Integrase in a Two-Protein-type V-C CRISPR System.
  JOURNAL   Mol Cell. 2019 Feb 21;73(4):727-737.e3. doi: 
            10.1016/j.molcel.2018.12.015. Epub 2019 Jan 29.
  PUBMED    30709710
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2018.12.015"; journalName: "Mol Cell"; date: 
            "2019-02-21- 21"; volume: "73"; issue: "4"; pages: "727-737.e3"
COMMENT     Sequence Label: pVC_CRISPR_Cat
FEATURES             Location/Qualifiers
     source          1..3246
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        119..221
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             311..970
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(377..396)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      991..1085
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     1182..1204
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1242..1260)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1328..1432
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1433..1501,1502..2293)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1651..1670)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2464..3052
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2953..2972
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3206..3223
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
       61 cgactggaaa gcgggcagtg agcgcaacgc aatcctctag aggatccaaa tgagacgttg
      121 atcggcacgt aagaggttcc aactttcacc ataatgaaat aagatcacta ccgggcgtat
      181 tttttgagtt atcgagattt tcaggagcta aggaagctaa aatgtcccca ttattggaag
      241 ggtttataag gaaagtaaag aagacctgct ttctagacgt ccattgttga tccgggaagg
      301 atcggtcttc atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca
      361 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt
      421 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc
      481 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaatttc gtatggcaat
      541 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga
      601 gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct
      661 acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg
      721 gtttattgag aatatgtttt tcgtgtcagc caatccctgg gtgagtttca ccagttttga
      781 tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta
      841 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
      901 tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg
      961 cggggcgtaa tttgatatcg agctcgcttg gactcctgtt gatagatcca gtaatgacct
     1021 cagaactcca tctggatttg ttcagaacgc tcggttgccg ccgggcgttt tttattggtg
     1081 agaatcctta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     1141 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     1201 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     1261 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     1321 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     1381 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     1441 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     1501 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     1561 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     1621 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     1681 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     1741 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     1801 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     1861 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     1921 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     1981 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     2041 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     2101 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     2161 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     2221 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     2281 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     2341 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     2401 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     2461 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     2521 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     2581 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     2641 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     2701 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     2761 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     2821 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     2881 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     2941 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     3001 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     3061 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     3121 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     3181 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     3241 aatacg
//
