LOCUS       sequence-235291-        2920 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid containing shortened version of upstream V-C CRISPR array 
            for integration assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Wright AV, Wang JY, Burstein D, Harrington LB, Paez-Espino D, 
            Kyrpides NC, Iavarone AT, Banfield JF, Doudna JA
  TITLE     A Functional Mini-Integrase in a Two-Protein-type V-C CRISPR System.
  JOURNAL   Mol Cell. 2019 Feb 21;73(4):727-737.e3. doi: 
            10.1016/j.molcel.2018.12.015. Epub 2019 Jan 29.
  PUBMED    30709710
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2018.12.015"; journalName: "Mol Cell"; date: 
            "2019-02-21- 21"; volume: "73"; issue: "4"; pages: "727-737.e3"
COMMENT     Sequence Label: pVC-CRISPR1
FEATURES             Location/Qualifiers
     source          1..2920
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        60..164
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(165..233,234..1025)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(383..402)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1196..1784
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1685..1704
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1938..1955
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     2404..2420
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2404..2420
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_feature    2433..2489
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2490..2507)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2490..2506)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2499..2521)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2715..2734)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2834..2856
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2894..2912)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
       61 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
      121 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
      181 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
      241 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
      301 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
      361 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
      421 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
      481 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
      541 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
      601 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
      661 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
      721 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
      781 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
      841 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
      901 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
      961 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     1021 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     1081 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     1141 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     1201 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     1261 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     1321 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     1381 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     1441 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     1501 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     1561 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     1621 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     1681 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     1741 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     1801 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     1861 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     1921 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     1981 aaccgctacg tgccgatcaa cgtatcattt tcgccagata tcgacgaaga gccttcatca
     2041 cagaaagctt gaggaagagc gtctccggag tccggagacg ctgcaatgat ggtcatatac
     2101 gataaattag ggacgcagaa gaccgatcct tcccggatca acaatggacg tctagaaagc
     2161 aggtcttctt tactttcctt ataaaccctt ccaataatgg ggagaccacg acaggtctcc
     2221 cttataaacc cttccaataa tgggccagag tttggtattg atccttataa acccttccaa
     2281 taatgggaga ctagcaaata tctatttgaa agtcaattat aaaagtataa taaatttgtt
     2341 taatatgtga ttaaacttga tagaaaatat attaaaaagt taaataaata taacaatttc
     2401 acacaggaaa cagctatgac catgattacg ccaagcttgc atgcctgcag gtcgactcta
     2461 gaggatcccc gggtaccgag ctcgaattca ctggccgtcg ttttacaacg tcgtgactgg
     2521 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     2581 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     2641 gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     2701 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     2761 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     2821 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     2881 gcgagacgaa agggcctcgt gatacgccta tttttatagg
//
