LOCUS       sequence-235293-        3469 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid containing shortened versions of both natural V-C CRISPR 
            arrays for preparing sequencing library.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Wright AV, Wang JY, Burstein D, Harrington LB, Paez-Espino D, 
            Kyrpides NC, Iavarone AT, Banfield JF, Doudna JA
  TITLE     A Functional Mini-Integrase in a Two-Protein-type V-C CRISPR System.
  JOURNAL   Mol Cell. 2019 Feb 21;73(4):727-737.e3. doi: 
            10.1016/j.molcel.2018.12.015. Epub 2019 Jan 29.
  PUBMED    30709710
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2018.12.015"; journalName: "Mol Cell"; date: 
            "2019-02-21- 21"; volume: "73"; issue: "4"; pages: "727-737.e3"
COMMENT     Sequence Label: pVC-CRISPR
FEATURES             Location/Qualifiers
     source          1..3469
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      512..538
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     553..569
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     553..569
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_feature    582..638
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(639..656)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(639..655)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(648..670)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(864..883)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     983..1005
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1043..1061)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1129..1233
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1234..1302,1303..2094)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1452..1471)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2265..2853
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2754..2773
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3007..3024
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aaatacaata atatgactgt atttagcccc taatttattt agtaactgta gggattatga
       61 gttttagcct tataaaccct tccaataatg ggccagagtt tggtattgat ccttataaac
      121 ccttccaata atgggccggc acttctatct ggaaccttat aaacccttcc aataatggag
      181 aatagcaaat atcatattga ttgtcaataa aaaattagaa agtttgatgc taaattgaag
      241 aataaattaa ttggaaaaat ggattaaaac actacaaagc atgaagttga ggattcggct
      301 attttcagcc gagagcgtag aggtaaattc tatcgctgag acgattgaca ataagagtgt
      361 agtttaagtt tctctctaaa gggaaaagtg acaattcttc ggctgcaaga gacaaaaagg
      421 attgtcctca ttgatttact ttaggtaaaa ttaccagatt agggttctgt tggactcttc
      481 taactttcgg agaaaaaact agagtcacac tggctcacct tcgggtgggc ctttctgcat
      541 aacaatttca cacaggaaac agctatgacc atgattacgc caagcttgca tgcctgcagg
      601 tcgactctag aggatccccg ggtaccgagc tcgaattcac tggccgtcgt tttacaacgt
      661 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
      721 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
      781 ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
      841 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
      901 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
      961 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     1021 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     1081 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     1141 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     1201 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     1261 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     1321 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     1381 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     1441 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     1501 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     1561 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     1621 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     1681 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     1741 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     1801 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     1861 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     1921 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     1981 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     2041 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     2101 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     2161 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     2221 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     2281 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     2341 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     2401 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     2461 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     2521 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     2581 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     2641 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     2701 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     2761 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     2821 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     2881 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     2941 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     3001 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgctacgt
     3061 gccgatcaac gtatcatttt cgccagatat cgacgaagag ccttcatcac agaaagcttg
     3121 aggaagagcg tctccggagt ccggagacgc tgcaatgatg gtcatatacg ataaattagg
     3181 gacgcagaag accgatcctt cccggatcaa caatggacgt ctagaaagca ggtcttcttt
     3241 actttcctta taaacccttc caataatggg gagaccacga caggtctccc ttataaaccc
     3301 ttccaataat gggccagagt ttggtattga tccttataaa cccttccaat aatgggagac
     3361 tagcaaatat ctatttgaaa gtcaattata aaagtataat aaatttgttt aatatgtgat
     3421 taaacttgat agaaaatata ttaaaaagtt aaataaatgg tgaggggct
//
