LOCUS       sequence-235316-       12940 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli-C. difficile shuttle vector for CRISPR interference 
            (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt 
            Pgdh::sgRNA-rfp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12940)
  AUTHORS   Muh U, Pannullo AG, Weiss DS, Ellermeier CD
  TITLE     A xylose-inducible expression system and a CRISPRi-plasmid for 
            targeted knock-down of gene expression in Clostridioides difficile.
  JOURNAL   J Bacteriol. 2019 Feb 11. pii: JB.00711-18. doi: 
            10.1128/JB.00711-18.
  PUBMED    30745377
REFERENCE   2  (bases 1 to 12940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Bacteriol. 2019 Feb 11. pii: JB.00711-18. doi: 10.1128/JB.00711-18."
COMMENT     Sequence Label: pIA33
FEATURES             Location/Qualifiers
     source          1..12940
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1788..1814,1815..1817,1818..4304,4305..4307,
                     4308..5894)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     misc_RNA        6788..6863
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             complement(7258..7629)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            7662..7771
                     /note="incP origin of transfer"
     primer_bind     complement(8967..8984)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9138..9726)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9218..9237)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgttatat gcacaactac tcccaacata aacatatatt gcttgttcta actcatattt
       61 ttgtaaaatt tcaacatcat atccagtcaa atcgtactct ctcttatata caaatggttt
      121 ttctaatttt ttgttcaata aatctcctcc tatttcttac ttaatttagc taaaaatcta
      181 tttatatttc acctcctttt tgactttaag cctacgaata ccatatttat gattataaca
      241 tcttctattt ttcccaaatc cttacatctc ccctaatttt tagacttaag ggcgaattct
      301 gcatcaagct agcataaaaa taagaagcct gcatttgcag gcttcttatt tttatggtac
      361 gtataattta aagggttatg gctgtatata gtcataaccc ttatatttca ttaattaaag
      421 ttaaattttg tatattcaaa aagctcttga tatttacggc agagccacca tatagagatg
      481 cctcagaccc taatgatgaa atttctatat ttattccttt gttaaaagag ctaactaaca
      541 tatcttttgt aatctgtaat atctcaggga tatcactaat tatttggcta tttaaatata
      601 ttatttcagg agcataagtt gtaatagcat tatttatagc tattgttaag taactacaaa
      661 actcatgaat aacttttttg gcattctggt tatcttcata atatagttgc tttactatat
      721 cagaatctat tttaggaata ttttctaaag aagaaagctg ttcaaatacc tttttttctg
      781 aacaatactg ttctaaacag ccacgatttc cacaaggaca tagtttacca ttaggcatga
      841 tgatagtgtg accaatttct ccactcatac catttctgcc actatataat ttattattta
      901 ttataatgcc tgaaccaaat ccactgtgaa tactgagact aagtagtgag ttatgtacag
      961 ttgaaaaagt attctcagct aaagctgtta agtttgcttc attttctatg tgaataggaa
     1021 agtcatactt tttgctaagg atgctatata agtcaatctc attaagattg taatatggag
     1081 taaacaatac tttattttca caagtaatcc catgtatagc caaagtaaga ccaattacct
     1141 tataaggagt gtctattttg gatatgttat aactatttat aatttcatct atcaattgta
     1201 taacattgtc tttacttact tgtatatctg ttaatttctt agaatttata atagttccat
     1261 ctaagtaaga tagagaagaa aatatatagt cgtatccaat gtccatactt aaagaaatcc
     1321 ctgcacattt attaaatact aataatatgg gttttcttcc gccactatga gtactatttc
     1381 caattcctat ttcatgaact agagattcat caataagttt tttggtaata gcggatatag
     1441 ttgctttatt caatcctata gtagaagcta tacttgccct agaaatagga ccatttttta
     1501 taatttgttc aagtaccaat ctttcattca tttctcgaat agtgtattta tcagtaacca
     1561 atttgatatt cctccttaaa ataatattgt aatacttttt acacaaaaat aaaaggttat
     1621 tttgcattga caaagataat taaatatttt attattagtt cataagttag tttaatatac
     1681 taacaaaaat aaagcaagta aaatatacct aaaatataaa aaaattagga taggaaaacg
     1741 atagttatga agtggcattc aaggagggta aagaggagag tcgacgcatg gataaaaaat
     1801 atagtatagg attagcaata ggaacaaata gtgtaggatg ggcagtaata actgatgaat
     1861 ataaagtacc aagtaaaaaa tttaaagtat taggaaatac agatagacat agtataaaaa
     1921 aaaatttaat aggtgcatta ctttttgata gtggtgaaac agcagaagca acaagactta
     1981 aaagaacagc aagaagaaga tatactagaa gaaaaaatag aatatgttat ttacaagaaa
     2041 tatttagtaa tgaaatggca aaagtagatg atagtttttt tcatagatta gaagaaagtt
     2101 ttttagtaga agaagataaa aaacacgaaa gacatccaat atttggaaat atagtagatg
     2161 aagtagcata tcatgaaaaa tatccaacaa tatatcattt aagaaaaaaa cttgtagata
     2221 gtacagataa agcagattta agacttatat atcttgcatt agcacatatg ataaaattta
     2281 gaggacattt tcttatagaa ggtgatttaa atccagataa tagtgatgta gataaacttt
     2341 ttatacaatt agtacaaaca tataatcaat tatttgaaga aaatccaata aatgcaagtg
     2401 gtgtagatgc aaaagcaata ttaagtgcaa gattaagtaa aagtagaaga ttagaaaatc
     2461 ttatagcaca attacctgga gaaaaaaaaa atggattatt tggtaatctt atagctttaa
     2521 gtttaggatt aacaccaaat tttaaatcaa attttgatct tgcagaagat gcaaaacttc
     2581 aattaagtaa agatacatat gatgatgatt tagataattt acttgcacaa ataggtgatc
     2641 aatatgcaga tttatttctt gcagcaaaaa atctttctga tgcaatactt ttaagtgata
     2701 tacttagagt aaatacagaa ataacaaaag caccattatc tgcaagtatg ataaaaagat
     2761 atgatgaaca tcatcaagat ttaacattac ttaaagcatt agtaagacaa caattaccag
     2821 aaaaatataa agaaattttt tttgatcaaa gtaaaaatgg atatgcagga tatatagatg
     2881 gtggtgcaag tcaagaagaa ttttataaat ttattaaacc aatattagaa aaaatggatg
     2941 gaacagaaga attattagta aaacttaata gagaagattt acttagaaaa caaagaacat
     3001 ttgataatgg aagtatacca catcaaatac atttaggtga attacatgca atattaagaa
     3061 gacaagaaga tttttatcca tttcttaaag ataatagaga aaaaattgaa aaaatactta
     3121 catttagaat accatattat gtaggaccat tagcaagagg aaatagtaga tttgcttgga
     3181 tgacaagaaa aagtgaagaa acaataacac catggaattt tgaagaagta gtagataaag
     3241 gagcatctgc acaaagtttt atagaaagaa tgacaaattt tgataaaaat cttccaaatg
     3301 aaaaagtatt accaaaacat agtttattat atgaatattt tacagtatat aatgaattaa
     3361 caaaagtaaa atatgtaaca gaaggtatga gaaaaccagc atttttaagt ggtgaacaaa
     3421 aaaaagctat agtagattta ttatttaaaa caaatagaaa agtaacagta aaacaattaa
     3481 aagaagatta ttttaaaaaa attgaatgtt ttgatagtgt agaaatatct ggtgtagaag
     3541 atagatttaa tgcaagttta ggaacatatc atgatttact taaaataata aaggataaag
     3601 attttttaga taatgaagaa aatgaagata ttttagaaga tatagtatta acattaactt
     3661 tatttgaaga tagagaaatg atagaagaaa gattaaaaac ttatgctcat ttatttgatg
     3721 ataaagtaat gaaacaactt aaaagaagaa gatatacagg ttggggaaga ttaagtagaa
     3781 aacttataaa tggaataaga gataaacaaa gtggaaaaac aatattagat tttcttaaaa
     3841 gtgatggatt tgcaaataga aattttatgc aattaataca tgatgatagt ttaacattta
     3901 aagaagatat tcaaaaagca caagtaagtg gacaaggtga tagtttacat gaacatatag
     3961 caaatttagc aggatctcca gcaataaaaa aaggtatatt acaaacagta aaagtagttg
     4021 atgaattagt aaaagtaatg ggtagacata aaccagaaaa tatagttata gaaatggcaa
     4081 gagaaaatca aacaacacaa aaaggacaaa aaaatagtag agaaagaatg aaaagaatag
     4141 aagaaggtat aaaagaatta ggaagtcaaa tacttaaaga acatccagta gaaaatacac
     4201 aacttcaaaa tgaaaaactt tatttatatt atttacaaaa tggaagagat atgtatgtag
     4261 atcaagaatt agatataaat agattaagtg attatgatgt agatgcaata gtaccacaaa
     4321 gttttcttaa agatgattct atagataata aagtattaac aagatctgat aaaaatagag
     4381 gtaaaagtga taatgtacca agtgaagaag ttgtaaaaaa aatgaaaaat tattggagac
     4441 aattacttaa tgcaaaactt ataacacaaa gaaaatttga taatcttaca aaagcagaaa
     4501 gaggtggatt atctgaatta gataaagctg gatttataaa aagacaatta gtagaaacta
     4561 gacaaataac aaaacatgta gcacaaatat tagatagtag aatgaataca aaatatgatg
     4621 aaaatgataa gcttataaga gaagtaaaag ttataacatt aaaaagtaaa ttagtaagtg
     4681 attttagaaa agattttcaa ttttataaag taagagaaat aaataattat catcatgcac
     4741 atgatgcata tcttaatgca gtagtaggaa cagcattaat aaaaaaatat cctaaattag
     4801 aatctgaatt tgtatatgga gattataaag tatatgatgt aagaaaaatg atagcaaaaa
     4861 gtgaacaaga aataggaaaa gcaacagcaa agtacttttt ttattctaat ataatgaatt
     4921 tttttaagac agaaataact ttagcaaatg gtgaaataag aaaaagacct ttaatagaaa
     4981 ctaatggtga aacaggtgaa atagtatggg ataaaggtag agattttgca acagtaagaa
     5041 aagtattaag tatgccacaa gtaaatatag taaaaaaaac agaagtacaa actggaggat
     5101 ttagtaaaga aagtatactt ccaaaaagaa attcagataa acttattgca agaaaaaaag
     5161 attgggatcc aaaaaaatat ggtggatttg atagtccaac agtagcatat agtgtattag
     5221 tagtagcaaa agtagaaaaa ggtaaatcta aaaaacttaa aagtgtaaaa gaattacttg
     5281 gaataacaat aatggaaaga agtagttttg aaaaaaatcc tattgatttt ttagaagcaa
     5341 aaggatataa agaagttaaa aaagatttaa taataaaact tccaaaatat tctttatttg
     5401 aattagaaaa tggaagaaaa agaatgttag caagtgctgg tgaattacaa aaaggtaatg
     5461 aattagcatt accatctaaa tatgtaaatt ttctttattt agcaagtcat tatgaaaaac
     5521 ttaaaggatc tcctgaagat aatgaacaaa aacaattatt tgtagaacaa cataaacatt
     5581 atttagatga aataattgaa caaataagtg aattttctaa aagagtaata ttagcagacg
     5641 caaatttaga taaagtatta tctgcttata ataaacatag agataaacca ataagagaac
     5701 aagcagaaaa tataatacat ctttttacat taacaaattt aggtgcacca gcagcattta
     5761 aatattttga tacaacaata gatagaaaaa gatatacaag tacaaaagaa gtattagacg
     5821 caacattaat acatcaaagt ataacaggat tatatgaaac aagaatagat ttatctcaat
     5881 taggtggtga ttaactcgag caaaaacatc gtagaaatac ggtgtttttt gttaccctaa
     5941 gtttcataaa aataagaagc ctgcaaatgc aggcttctta tttttatggt ttaaacggtt
     6001 ttagctggga tatcggcaaa acaagcaaat actactatag aagcagttaa aagggctatt
     6061 ttttataatc tggagcatag acaagaagtt agagaagcaa ttaaaaaaga aaatataaga
     6121 aaaattctta ttattgggac atcaaaaaaa atggttaatc aaattgtaga gagattgtca
     6181 attggaaggg tctgtaagtt tataaatata caagatataa gtactaaata tgaaatagaa
     6241 atagcaaaac aagctagaaa agaaggaaat catgtaattc ctgttccagc cgttgagata
     6301 aaaagtatgg caagtggtct cagtataaat tctttgaaga gactttttag aaaaggaaat
     6361 aataggaata tggtagtttt agaaaaaaca attataagac caacttttag ttatatagga
     6421 aagttttata ttagtgctga tgtaattaaa cagattattg aatatgaagt atacaacttt
     6481 ggaagcattg atagaataaa taaaatcaat atagagaatt gcaataattt tatgaatatt
     6541 tttgtaagta taaatatcaa tgatttagaa actataaaaa aaattgaaga catacaacag
     6601 acaataaaaa agtctattga gaaaatgact ttagttaatg tacaaaaaat agatattaac
     6661 attcataaaa ttaaaaaaat aaacttcctc caatgttaaa aaataaactg agaaaatgat
     6721 atactaattt gcaataaagt gtgctataat taaactgtaa atggccaaac tttcagttta
     6781 gcggtctgtt ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg
     6841 aaaaagtggc accgagtcgg tgcttttttt ctatggagaa atctagatca gcatgatgtc
     6901 tgactagcgg ccgcggatcc tataagtttt aataaaactt taaatagaaa aaggcttctc
     6961 tcatgagaag tcttttttat ttaaaataaa tataaaataa aatagaggct ataaatagcc
     7021 tctattttat gtgagaaatc cctaaataaa aagatgccag tgtgctggaa ttcgccctta
     7081 ggctgcgcta gggacctctt tagctccttg gaagctgtca gtagtagggg atcggtcttg
     7141 ccttgctcgt cggtgatgta cttcaccagc tccgcgaagt cgctcttctt gatggagcgc
     7201 atggggacgt gcttggcaat cacgcgcacc ccccggccgt tttagcggct aaaaaagtca
     7261 tggctctgcc ctcgggcgga ccacgcccat catgaccttg ccaagctcgt cctgcttctc
     7321 ttcgatcttc gccagcaggg cgaggatcgt ggcatcaccg aaccgcgccg tgcgcgggtc
     7381 gtcggtgagc cagagtttca gcaggccgcc caggcggccc aggtcgccat tgatgcgggc
     7441 cagctcgcgg acgtgctcat agtccacgac gcccgtgatt ttgtagccct ggccgacggc
     7501 cagcaggtag gccgacaggc tcatgccggc cgccgccgcc ttttcctcaa tcgctcttcg
     7561 ttcgtctgga aggcagtaca ccttgatagg tgggctgccc ttcctggttg gcttggtttc
     7621 atcagccatc cgcttgccct catctgttac gccggcggta gccggccagc ctcgcagagc
     7681 aggattcccg ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg aacacccgct
     7741 cgcgggtggg cctacttcac ctatcctgcc cggctgacgc cgttggatac accaaggaaa
     7801 gtctacacga accctttggc aaaatcctgt atatcgtgcg aaaaaggatg gatataccga
     7861 aaaaatcgct ataatgaccc cgaagcagga agggcgaatt ctgcagtgca gatctgaccg
     7921 gtctctgaaa atataaaaac cacagattga tactaaaacc ttggttgtgt tgcttttcgg
     7981 ggcttaaatc aaggaaaaat ccttgtttta agcctttcaa aaagaaacac aaggtctttg
     8041 tactaacctg tggttatgta taaaattgta gattttaggg taacaaaaaa caccgtattt
     8101 ctacgatgtt tttgcttaaa tacttgtttt tagttacaga caaacctgaa gttaactatt
     8161 tatcaattcc tgcaattcgt ttacaaaacg gcaaatgtga aatccgtcac atactgcgtg
     8221 atgaacttga attgccaaag gaagtataat tttgttatct tctttataat atttccccat
     8281 agtaaaaata ggaatcaaat aatcatatcc tttctgcaaa ttcagattaa agccatcgaa
     8341 ggttgaccac ggtatcatag atacattaaa aatgttttcc ggagcatttg gctttccttc
     8401 cattctatga ttgtttccat accgttgcgt atcactttca taatctgcta aaaatgattt
     8461 aaagtcagac ttacactcag tccaaaggct ggaaaatgtt tcagtatcat tgtgaaatat
     8521 tgtatagctt ggtatcatct catcatatat ccccaattca ccatcttgat tgattgccgt
     8581 cctaaactct gaatggcggt ttacaatcat tgcaatataa taaagcattg caggatatag
     8641 tttcattccc ttttccttta tttgtgtgat atccacttta acggtcatgc tgtaggtaca
     8701 aggtacactt gcaaagtagt ggtcaaaata ctcttttctg ttccaactat ttttatcaat
     8761 tttttcaaat accatctaag ttccctctca aattcaagtt tatcgctcta atgaacaaag
     8821 atattatacc acatttttgt gaatttttca acttgcccac ttcgactgca ctcccgactt
     8881 aataacttct tgaacacttg ccgaaaaaga aaaactgccg ggtacgtacc cggggatcga
     8941 tccccggccg agcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     9001 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     9061 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     9121 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     9181 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     9241 tgaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     9301 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     9361 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     9421 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     9481 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     9541 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     9601 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     9661 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     9721 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     9781 cgttaaggga ttttggtcat gaaatgcaag tttctaacta acatacatca tagttactaa
     9841 actatggtaa ctataatttt attaactata ttaagcactg attagtacta taactcaata
     9901 taagcatatc ccctgtatcg taactagaga accaaacgac ggaaaaagcg atatagataa
     9961 gtttagatag tgcaccaatt ttttactttt ctattttaga atctagcatt tccaatgctt
    10021 actaatacta tttttgattt ttaacttgtt ttaaaaaagc acctactaat taggattcta
    10081 gctctatatt ctagttatac attctctata ttctagctct atattctagt tatacattct
    10141 ctatattcta gctctatatt ctagttatac attctctata ttctagctct atattctagt
    10201 tatacattct ctatattcta gctctatatt ctagttatac attctctata ttctagctct
    10261 atattctagt tatacattct ctatattcta gctctatatt ctagttatac attctctaaa
    10321 aaacatatat atatatacta catatatatg tacaattatt ttaccaaaag aaatttttaa
    10381 aacggcttaa tttcaatact ttgatgccat tttttttttg caattctcgt acaattattt
    10441 taccaaaaga aatttttatt aattagtttt tttgcataaa aataggacta gaaaatcaat
    10501 tctaatccta tcaaaaatct acttgtttaa tctatatgta tagtacataa atcctctatc
    10561 atttatttta gtttcccata catctaaaat tttagtatct aataaagatt ctatcatatt
    10621 tgaaattctt tgctgtgtta ctcctaaatc ttttgctaat tcctcttgat taattcttaa
    10681 tatatttcct tttattttat catctttgac caataaatct tgcttatatc tcatatgagt
    10741 atataatctt aattcttcag ttgaaattat tccccaaatt actgccatgg taacaaagta
    10801 actaatagta tattgtttat ctatattaca tctaacttct tttacattgt atgtatcttt
    10861 tattcctagc tttgtaacac cttttgtttt tgtaatataa tcattatgtt gaagttcttt
    10921 taatgtttct cttaatgtct tttcactcat aacaatgttt ttaactttct tcttacgttt
    10981 ataggttatc agctccatta tatcgtctat gtttaattct ctatctttat tgttaagtaa
    11041 cacattatag ataaacaatt gattaccagt cattatttta acccctttcc tattcttata
    11101 tttcaaatcc tttgagtgct tatgtggcat attgaacaaa gtatcttcat cactatatat
    11161 aaaatctgat tctattttat tccaacaatc actattaaac tcacaacctg tacaaccaaa
    11221 attaaccgtt ttaagcttct catacatata cttgacttga tattctagct cactatttga
    11281 aagactattt tgtgatatat agttccattc tctagcaacg gatattactt gacttaaact
    11341 tttattcctc aatcttaaag tcacaactat tttttgcaaa tctaggtttt tatgtccatc
    11401 tttactacca ttttttaaaa tatctactat acaatttggt agcttactat ctaatatata
    11461 ttttgtatta gtattaccta ctgtttttac atctttgcaa tattttttag ctaacttttc
    11521 tatgtcatat ctatatatat ttttgtcttg gtgtattatt tttacttgtt tagtagtatt
    11581 tttaatatta tatgtataag gtactcttaa tacttgagta gataaatttg cttgtttatc
    11641 tgcacccaat ttatttatta atgtatttgt aacttctgat actaacttaa tgttattagt
    11701 tttattaatt agcacataaa catggaatcc atttccacta tcaactagga tatgataatg
    11761 taatcctatc ttagtaaata aattaattat atctttataa ttaaaacctt gtcctaattc
    11821 ttttttgtca aaatcaaatc ctaatgcata tctatttatt aagttttcat tggttcctgc
    11881 ttttccatca gttgtacata ggttaaaaaa tgtattttca taataaatat tttttgcgaa
    11941 ggtaaattct actatatcat ctatattttt aaaaaaagtt gttttactat attctttaaa
    12001 ttcttctccg agttctaatt gtttactatt acttttatta ttttgaaata ctctaacata
    12061 ttcattatca ataagattat ttccttgtcc tataccatct ctaaatactg ccattagaaa
    12121 ttttttcaac ttatattttg aatctaattg ttccatatat ttattatctc cttatctagt
    12181 ttttttaaga gataacacta cattaaatat atacttattg actaatcaac ttttacaact
    12241 attgaataat tttagcttag tatgtataat aatattgtta agatgctaaa attgatagca
    12301 atattattat ataactacag atacttaatc agttagaccg atattggagt tggtagctcc
    12361 ataaactcaa ttggtagttg agttgtcatt gtctagtagt attatctcat tttgttgtta
    12421 tcttaaaata ttattttaaa aatactctgc ttggtcggct tagggtattt tttttgttta
    12481 attccaatat tggttcaaac tcctgtgtaa tacttttgac acttccatct gtattgaatt
    12541 gacagttgat atggtatctc attattggct tgttcttatg tttttctatg tatttatttt
    12601 ttactttcaa ataatttgta tttccatttt tagttatgtt taacacacta tccttattac
    12661 tcaataaaat tattttttgt tttaacatat gatttataga atctaaaaat tgatattgac
    12721 ttataatagt actttctacc tctctagctt tttctagtgt ttcaaattct tcaaactctg
    12781 ctatattgct atagtatagc ttatcattaa taatattatc tgacaacttt aattccattt
    12841 tataaaacaa ttccaagtta tgtataaaat ttttaatgtt acaacatata gacttattat
    12901 ttgttcttat atggtatctc catttactac ttctagctct
//
