LOCUS       sequence-235336-        2108 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Outer Mitochondrial Membrane.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2108)
  TITLE     Davidson HaloTag constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Halo-TOMM20-N-10
FEATURES             Location/Qualifiers
     source          1..2108
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        33..132
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     83..103
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      160..169
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      625..634
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             631..1521
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     polyA_signal    1646..1767
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1683..1702)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1737..1756
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1911..1932)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
       61 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      121 ataagcagag ctggtttagt gaccgtcaga tccgctagcg ccaccatggt gggtcggaac
      181 agcgccatcg ccgccggtgt atgcggggcc cttttcattg ggtactgcat ctacttcgac
      241 cgcaaaagac gaagtgaccc caacttcaag aacaggcttc gagaacgaag aaagaaacag
      301 aagcttgcca aggagagagc tgggctttcc aagttacctg accttaaaga tgctgaagct
      361 gttcagaagt tcttccttga agaaatacag cttggtgaag agttactagc tcaaggtgaa
      421 tatgagaagg gcgtagacca tctgacaaat gcaattgctg tgtgtggaca gccacagcag
      481 ttactgcagg tcttacagca aactcttcca ccaccagtgt tccagatgct tctgactaag
      541 ctcccaacaa ttagtcagag aattgtaagt gctcagagct tggctgaaga tgatgtggaa
      601 ggcggtagcg gggatccacc ggtcgccacc atggcagaaa tcggtactgg ctttccattc
      661 gacccccatt atgtggaagt cctgggcgag cgcatgcact acgtcgatgt tggtccgcgc
      721 gatggcaccc ctgtgctgtt cctgcacggt aacccgacct cctcctacgt gtggcgcaac
      781 atcatcccgc atgttgcacc gacccatcgc tgcattgctc cagacctgat cggtatgggc
      841 aaatccgaca aaccagacct gggttatttc ttcgacgacc acgtccgctt catggatgcc
      901 ttcatcgaag ccctgggtct ggaagaggtc gtcctggtca ttcacgactg gggctccgct
      961 ctgggtttcc actgggccaa gcgcaatcca gagcgcgtca aaggtattgc atttatggag
     1021 ttcatccgcc ctatcccgac ctgggacgaa tggccagaat ttgcccgcga gaccttccag
     1081 gccttccgca ccaccgacgt cggccgcaag ctgatcatcg atcagaacgt ttttatcgag
     1141 ggtacgctgc cgatgggtgt cgtccgcccg ctgactgaag tcgagatgga ccattaccgc
     1201 gagccgttcc tgaatcctgt tgaccgcgag ccactgtggc gcttcccaaa cgagctgcca
     1261 atcgccggtg agccagcgaa catcgtcgcg ctggtcgaag aatacatgga ctggctgcac
     1321 cagtcccctg tcccgaagct gctgttctgg ggcaccccag gcgttctgat cccaccggcc
     1381 gaagccgctc gcctggccaa aagcctgcct aactgcaagg ctgtggacat cggcccgggt
     1441 ctgaatctgc tgcaagaaga caacccggac ctgatcggca gcgagatcgc gcgctggctg
     1501 tcgacgctcg agatttccgg ctaagcggcc gcgactctag atcataatca gccataccac
     1561 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     1621 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     1681 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     1741 tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat attttgttaa
     1801 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     1861 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     1921 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     1981 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     2041 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     2101 cggcgaac
//
