LOCUS       sequence-235338-       15698 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  miniMos vector expresses ERKKTR-mClover and mCherry-H2B in C. 
            elegans VPCs; request with plasmids pCC335 and pCC336 for 
            unphosphorylated and phosphomimetic controls (Contains Neomycin 
            resistance).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15698)
  AUTHORS   de la Cova C, Townley R, Regot S, Greenwald I
  TITLE     A Real-Time Biosensor for ERK Activity Reveals Signaling Dynamics 
            during C. elegans Cell Fate Specification.
  JOURNAL   Dev Cell. 2017 Sep 11;42(5):542-553.e4. doi: 
            10.1016/j.devcel.2017.07.014. Epub 2017 Aug 17.
  PUBMED    28826819
REFERENCE   2  (bases 1 to 15698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2017.07.014"; journalName: "Dev Cell"; date: 
            "2017-09-11- 11"; volume: "42"; issue: "5"; pages: "542-553.e4"
COMMENT     Sequence Label: pCC324
FEATURES             Location/Qualifiers
     source          1..15698
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     283..299
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     283..299
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7873..7894)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     8481..8502
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             8512..8565
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(10697..10714)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10697..10713)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             11535..12329
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(11589..11608)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     12199..12218
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     13030..13046
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     13031..13047
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13702..13724
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(13762..13780)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        13848..13952
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(13953..14021,14022..14813)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(14171..14190)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      14984..15572
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15473..15492
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcaccgtacg tctcgaggaa ttctatcagg tgtacaagta tgaaatgtcg tttcgaacat
       61 atagatgtct cgcaaacgta aatatttatc gattgtcata aaactttgac cttgtgaagt
      121 gtcaaccttg actgtcgaac caccatagtt tggcgcgaat tgagcgtcat aattgtttac
      181 tctcagtgca gtcaacatgt cgagtttcgt gccgaataaa gagcaaacgc ggacagtatt
      241 aattttctgt tttcatttga agaaaacagc tgcggccgga tccaggaaac agctatgacc
      301 atgattacgc caagctacgt aatacgactc actagcagag cgaattctga tctacaaata
      361 tattatggat cggtttccgt tttataggaa aaacacgcag aggtggcaaa actccctgcg
      421 acacaacttg tcattcaacg actgcttcat caagatccca agaagagccg atcgtcctgg
      481 gaagggctcc tactgggcgg tgcacccaaa tgcctccgga atgttcgaga acggcagctg
      541 cttacggcgt cggaaacgat ttcgggtaag tcttcggtta ctgtacttat caaattgtgt
      601 aaattaatga tcttatcact tcaaagagaa acgcaaaaat ctccaacaaa aaaaaacgaa
      661 aaattgattt agagagggcg cgcgcgtgct tcccccttca cgattttgag agagaaagac
      721 aattctacag taatcctcaa ggttggtcga tgacagaaaa ggcgagaaat ttgttttaaa
      781 tacgattgag agactattgg gggaagagat tatgatgaac ggctgcagga ttggataaga
      841 ctttgcagat tttgatgtgg ggtactgtag tttaaaggga agactgtgaa caaatattta
      901 agatagtgtg gctatgcgca ggcttaggct taaatttagg tttaggctta ggcttaggct
      961 tagtcttagg ctcagtctca gggttaggtt taggcttagg ctcaggttca gacttaggtt
     1021 caggcttagc ctattgctta ggcttaggct taggcttagg cttaggctcc ggcttaggat
     1081 taggcttagg cttaggttca ggctcagggt taggcttagg gtaaagctta ggttctggct
     1141 taggcttaga cttatgctta agattaggca atgaagagta cctgtgtatt tggttcagcc
     1201 gaaattgcgc aactttgaga atgtacaaaa ttcttaaaaa attgcagagt tggaactttc
     1261 agaaatcgca aatgccttcg gagaatagga agcttcggca ctgtggtttt ggtactgacc
     1321 tgcaattttc aaatcattct gcaaagtttt aaatttgaca aaaatttttt atcaaaatgg
     1381 ttttgaaacg tttatctaca tagggtaatt aacctcgccc cccccccccc cccaaaatat
     1441 tttcaatttt cactcaattt tactcaatac aattaaacag tcatattaag atgaattgac
     1501 ttacgcgcgc gaaatactct cctcccggta ctacccaata aaatgtgcca tctgttgcaa
     1561 tagttgtact cctactactt ctacgggggg gggcaacaga tgcctcctat ccaatattgt
     1621 agtgttatgt tgaaatgaaa atgagtgact tgtgtctgtg tgtgtatgat gaagtagtta
     1681 gaaaagagga caaagaaaaa gtaataagaa gttgcaagag tttgtttgag tggggtgagc
     1741 ggggtttgca cataacttta aatttttttc aactattcgg cttgggaact ctacacctat
     1801 tgaaatataa ggctgtaggc aagcacttag gcatgtaggt aggaagaacg gggacatgac
     1861 tatgcaaaca ggtataattc tgttccaatg tactgttcca aatatcaaat cctccataca
     1921 gtctgtcctc caaccacaag cccattaaaa tgacagtcag gctgtccagc ggggggtccc
     1981 ccgccgaaaa agaagggctg tcgcggggcg ctagctaata tgtgacacat atacatataa
     2041 ctgtatcact tgtacatgta cataagacgg attggaaaca aaagtgattc tctttgccta
     2101 tggttcattc catttcgcgc aatgttttag gtagtacggt agattaaatg gccttggggc
     2161 gaggagggga ggactaatca cttggagggg aaagaagagg caagacaaag attgatgcgg
     2221 tttgaattta gatatttagg tggtggagcg aggtgtcaat gtttgtcagg tgcctgattt
     2281 attagtatat gtactttaag agatgaatta tagaagtgct gcaaatttga aaggcataac
     2341 tgtaaggtac cttttaaatt actaccggca tgtggtgtca aagtgtttca gttcggtttg
     2401 atctacaaag aatgctggag aagagacgca gactttttcg gggcggtgct cgggaagagc
     2461 tgatttacgt cgatttacag ttttcctcgt ttctagaatt tcgtaaatcg acacaaatcg
     2521 gctttttccg cgccccgtcc tttgggtccc gggatgtctc tgtctcttca actatctaca
     2581 ctatctgtac tggtaatagt gcattttcta aaattgaatc ataaaatttt gctgtaaaag
     2641 ttgaacgttt tttaaacaca gttttaaaaa atatttaaaa aattcaattt caggctcgcg
     2701 gcggccaaga cgacgacgac gacgacttcc accacccggc tccatcgaaa atctctcgca
     2761 aaaatcctct tccactgctc ccggagcccc caataacccc accactcctc tcctcccttt
     2821 tcccaaatct tccaccatca ctcccaaatt tctgcctgtt tcctccaggc atggatcgat
     2881 cgtagagcac tagtggatcc agagcgcatg ctctagtttt gtaaatctac aatgttcaaa
     2941 cgctggactt catgctaaga tcctgaaaat aggcagtagg taggtaggtt cagccataaa
     3001 taccaaaaaa catgcctgac aggcctacct attcaggact acctacgcct gtctcgtgcc
     3061 tatatgtcta ttctactatt ttagtaaaca tacactcttc aaaaacgcaa tatcaccgaa
     3121 aatagcaaaa agtaagcgga aagcatgcat gtaggcaagt aggcagatag gaataggtca
     3181 tgtataaaaa catacctgta actacatgcc ttgtctattt ctcaaagncc cccccccccc
     3241 attgttgacg aaaattgaca ccgacaattg ccctattctt tctccttatt tctctcctct
     3301 ccattctatt tcataacttt tcatcagtat attcactcaa ttggaagaaa atatgtatgt
     3361 ctacaacaac ctacaagaag acgtcaattt catttcggac cccccttctc cacgtttgtc
     3421 gagaaagtcg taaagagcat atggaggagc aacaaggggg cttagtttca cattttaatt
     3481 gttgcgcctt cttgttgacc tcctggacag gaaaatggat gagtttatga tgttgggaga
     3541 aacaaatttt tggaattttt aatgaaaacc tatttggaaa gtcctgaaga cctaacgttt
     3601 gaattttttg agaaaaaaat gtacaaaaaa tacagtttat tctagtggct tgaaaggcaa
     3661 attcaaactc ccgccaaaga aaggcaccgt ccaatcatcg acgccggcca cacccactgc
     3721 tcattgctga ttcgtcgata gggagtttct gtagaagaat ataaaggtac tgtaggcaac
     3781 tgtagcggta ctgtagaaga gtgtggatgt actgttggtg taatgtagag gtactataga
     3841 tattctctag gggtactgta tgaaaatgta tggattctgt ggaaatattg taggaaactg
     3901 tgggggtact gaagatatac tgtagatgtt ctgtgtggtt tttaaggggt actgtaggaa
     3961 tactgaagag cgttttttgc aatagaatcc gctcatatct tgactatccc agcattgtcg
     4021 gatttgtttt tcaaaaatca ttttcttaaa gctattttct ctggaaaaaa ctcttcccaa
     4081 ttaccgtacc agtgtcattt ttccttactg aaaacaaagg aaaacaagta ggttgaggaa
     4141 agaaaaacaa ttgtttttaa tcttgaatga gtaagcttgg cacacacatg tctctaaaac
     4201 aaattaacta cggagtacgg tagtcttgtt tttcaaaaga gaaccaactg ggtacatatt
     4261 agttttcatt ttactccccc tccttgcatt ttcctcgttt tcataacaaa tcaatttgga
     4321 gtcatattca tggaaattga aaaagacaag aagacaagga gtaggagaag gtaatgagaa
     4381 aaatgggttt aaggttttga aaagaaattg acaatcacat aaaacctaga ggggtgctaa
     4441 gtaagcaatg tgatcggaaa ttgtaatgga ttagggtgaa tggagggtgt ttggttttgg
     4501 gtttgattga atttggcgct agttgccaag agttgccggg aatctggctt ttgctgggtg
     4561 gtaaaattca tagaaaaatt tgtagaaaaa tcggcggccg agtttttcct tttttgcgac
     4621 cacgcaggtt gcaaaaagca taaattctgg ttcatgttca caaaacctcg cgcaagtttt
     4681 taaaatcttg agtaggggcc tgcctgaggt gctgaggtga attagaccaa gtttttgttc
     4741 gcaactacca aaaacttcag aaaaattttt gttaatctca tcataaaatt cgtttacttc
     4801 ggctaatttt tattagttat aagtctataa aaaacctcca aaatatcccc tttttaaggt
     4861 taaatttttg aaaattttcg aattataata aagtctgaaa attgtcaaaa tttcctgcaa
     4921 ttgaccgtgt tggcaatttt ggcaatttca gcaagagccg aaattaccaa ttgcacggtc
     4981 tcgtcaaggt tgtcagtgtc tagataaaca ttatgaaaat ttcgaaaagc tgacgaaaac
     5041 tcttcagagt atatgactca aacttttgat gattttgatg cgacattgct caaagttaca
     5101 taaaaatcta attttaaaat gttataaagc ttaatccctt tctaattcct aataagtacc
     5161 gaaagtacag tagttctagt caccttgcca aaacaaaagg ataaacagga aaactaaaaa
     5221 gtttctaaaa agaaaagccg cgaaaaggag agagagagat gggataatgt tacaaattgg
     5281 acctgttaag tgtcggttta atgaaccgag cttctaaata tggcacgagg agcggtggtg
     5341 tggccagcgg cgccgcaccg tctgcagtca accgtgcgat cgagcggcac ctttgatcca
     5401 aaaaatgccg cgatttttgc cgcgctctcc ccaggcaggc agtggggaag agatgtcctt
     5461 tatgaaggag attgacggga ggaggaggag gaggaaaccg tttttgatta actggtggat
     5521 ggcattgcgg tttttattct tccgtaagga caactggttc ttttttcttc tattttgatg
     5581 tcttcgtttt gtctttactg gtctgtcttc gaagttgctg agctgattta gaacattgta
     5641 aatttgtaag aaaattgatt tttatcaaca taattacgaa gtatcacgtt ttacgtgtgt
     5701 ttttagagtt ttcaagtaaa aaatttgaaa tcagcggtat tcaaaaccta aaccttttca
     5761 cggggactcg atcccttgac cttttgctcc aaaggcattg ctcttaccca ctgagccatc
     5821 cgtggcgccg ttttcttagg gcgccgtacc aagtggcgca gtggctaaga gggacgactt
     5881 tgaagcaaac ggtcatgagt tcgagccccc gggagttgat ttaaattttc tgattacgac
     5941 tcgctgcgcc ctaaaggtcc ctttagctcc ccattgatca tttatcccaa atctcattta
     6001 taaaatcttt gtcccgagac aaacccagtc atatttaatt agaataatca ctaccctttt
     6061 ttgattctcc cacatacaat ttttttaaac cgagagtacg gtaggtaata acaatttatg
     6121 gagaagaata ttaggtgatg agaatgggtg tctctactac tattatatat attactttat
     6181 tcattcatta aattatggtg gtggtggtga cgatgagaaa ctttacggaa tttatgagtg
     6241 aaattgttcc aaaaaagtgg tgaagtgtca aatggaatat ctaactggga ggggggtgaa
     6301 taaggacaat ggggttagtg tactctcgag aatatggaat tagaaaatga ggaaaacttg
     6361 caaaaaatgg agctttgaaa aaaactagct gaaaacatcc tttcagagtt ggattttaag
     6421 attttaagat ttctctaaat cattgtagtg aaattttcta ctgaggtttt ctagtttctt
     6481 tttcatttgg tatacatttt tttgtagaaa atgttcacca aaactttttt cgaatgcata
     6541 ataattcaac attttctatg ctttttaaat tatttttctt gttgcagaaa acccaaacta
     6601 ccgtactttt tcactaggtg gtctccaaac tttaatccgt tataactcca caaatatttt
     6661 tgatataaaa aagttgtcaa ttaacaaaat gtgcaaaatt ttatgaccaa tatttaatca
     6721 gttgacagtt ttttgatatc tcaggtaacc gcagagttat gagcgtttga agttgaagcg
     6781 tgctctaggc tctccaggtg acccaaatat ttgaatattt tagtaattct catgatttta
     6841 aaattgtcca acaaaaattc aaatattgac actaattctt cccacctaga caccccacca
     6901 ttccatggac taatcccgtt aattcgaacg cttcccccct accgctctcg gtgtcactca
     6961 aaatttcaaa caagtcgtga cagttctcgg gtggtctgat accagtttac ctacattttc
     7021 aaaaaaaaac acaatcaact ctttcatctt cagtattcca gtgaatcccg tcccacacca
     7081 gatgccccaa ctcagcgggg gctgcgtgat acccggtcag cagcagcacc agcaacagga
     7141 gtaactggtt cctcccaaat ataggttagt gatgtgaatg taaaacaaag acttattttt
     7201 ctattagaaa tgtgttgtca ttttccttat tacaagttgt tgcctttttc ccttacgggc
     7261 ggtctcggta agcgctgtct ccccgttggc cagccccgcc cactcaacgc tccgcccttc
     7321 ttctccttct aaggcttggc ttctcaaaca aattgacttt tcccaatcgc taagatttca
     7381 atatattttg atatattcca aaaccttctt tattaattat caatccaagc aaaactctat
     7441 acatattccc tgaaaaagat ccccgggatt ggccaaagga cccaaaggta tgtttcgaat
     7501 gatactaaca taacatagaa cattttcagg aggacccttg gctagcgtcg acgccagatc
     7561 tgcggcggcc gcaccatgaa gggccgaaag cctcgggacc tggaacttcc acttagccca
     7621 agcctgctgg gtggccaggg acctgaacgg actccaggat caggaacaag ctctggtctt
     7681 caggcaccgg ggccagcgct ttcgcccagc aagcgtagcg gtctcgagga cccagcgacg
     7741 ccatcgaaga agccaaaaac cccatcggtg tcgtcaaggc tggagcgcct gaccctccag
     7801 tccagctttc aattcccatc cactagtatg gtgagcaagg gcgaggagct gttcaccggg
     7861 gttgtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtccgc
     7921 ggcgagggcg agggcgatgc caccaacggc aagctgaccc tgaagttcat ctgcaccacc
     7981 ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cgtggcctgc
     8041 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     8101 ggctacgtcc aggagcgcac catctctttc aaggacgacg gtacctacaa gacccgcgcc
     8161 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     8221 aaggaggacg gcaacatcct ggggcacaag ctggagtaca acttcaacag ccacaacgtc
     8281 tatatcacgg ccgacaagca gaagaacggc atcaaggcta acttcaagat ccgccacaac
     8341 gttgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     8401 ggccccgtgc tgctgcccga caaccactac ctgagccatc agtccgccct gagcaaagac
     8461 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgagggcaga
     8521 ggaagtctgc taacatgcgg tgacgtcgag gagaatcctg gcccaggagg tagggccggc
     8581 tctgtctcaa agggtgaaga agataacatg gcaattatta aagagtttat gcgtttcaag
     8641 gtgcatatgg agggatctgt caatgggcat gagtttgaaa ttgaaggtga aggagaaggc
     8701 cgaccatatg agggaacaca aaccgcaaaa ctaaaggtaa gtttaaacat atatatacta
     8761 actaaccctg attatttaaa ttttcaggta actaaaggcg gaccattacc attcgcctgg
     8821 gacatcctct ctccacagtt catgtatgga agtaaagctt atgttaaaca tccggcagat
     8881 ataccagatt atttgaaact ttcattcccg gagggtttta agtgggaacg cgtaatgaat
     8941 tttgaagacg gaggagttgt tacagtgacg caagactcaa ggtaagttta aacagttcgg
     9001 tactaactaa ccatacatat ttaaattttc agcctccaag atggagaatt tatttataaa
     9061 gtcaaacttc gaggaacgaa tttcccctcg gatggacctg ttatgcagaa gaagactatg
     9121 ggatgggaag cttcaagtga aagaatgtac cctgaagacg gtgctcttaa gggagagatt
     9181 aaacaacgtc ttaaattgaa agatggagga cattacgatg ctgaggtaag tttaaacatg
     9241 attttactaa ctaactaatc tgatttaaat tttcaggtga agacaactta caaagccaaa
     9301 aaaccagttc agctgccagg agcgtacaat gttaatatta aactggatat cacctcccac
     9361 aacgaggatt acactatcgt tgagcaatat gaaagagctg aagggcggca ctcgacaggt
     9421 ggcatggatg aattgtataa gggaggagga ggaggtggaa tgccaccaaa gccatctgcc
     9481 aagggagcca agaaggccgc caagaccgtt acgaagccaa aggacggaaa gaagagacgt
     9541 catgcccgta aggaatcata ctccgtctac atctaccgtg tcctcaagca agttcatcca
     9601 gacactggag tttcctccaa agccatgtct atcatgaact cttttgtcaa cgatgtcttc
     9661 gagcgtattg ctgctgaagc atcccgtctt gctcactaca acaagcgttc cacaatctca
     9721 tcccgcgaaa ttcagaccgc tgtccgtctg atccttccag gagagcttgc caagcacgcc
     9781 gtgtctgagg gaaccaaggc cgttaccaag tacacttcca gcaagtaagc ggccgccagc
     9841 tccgcatcgg ccgctgtcat cagatcgcca tctcgcgccc gtgcctctga cttctaagtc
     9901 caattactct tcaacatccc tacatgctct ttctccctgt gctcccaccc cctatttttg
     9961 ttattatcaa aaaacttctc ttaatttctt tgttttttag cttcttttaa gtcacctcta
    10021 acaatgaaat tgtgtagatt caaaaataga attaattcgt aataaaaagt cgaaaaaaat
    10081 tgtgctccct ccccccatta ataataattc tatcccaaaa tctacacaat gttctgtgta
    10141 cacttcttat gttttttact tctgataaat ttttttgaaa catcatagaa aaaaccgcac
    10201 acaaaatacc ttatcatatg ttacgtttca gtttatgacc gcaattttta tttcttcgca
    10261 cgtctgggcc tctcatgacg tcaaatcatg ctcatcgtga aaaagttttg gagtattttt
    10321 ggaatttttc aatcaagtga aagtttatga aattaatttt cctgcttttg ctttttgggg
    10381 tttcccctat tgtttgtcaa gatttcgagg acggcgtttt tcttgctaaa atcacaagta
    10441 ttgatgagca cgatgcaaga aagatcggaa gaaggtttgg gtttgaggct cagtggaagg
    10501 tgagtagaag ttgataattt gaaagtggag tagtgtctat ggggttcttc gaatgcatcg
    10561 cgcgcaccgt acgtctcgag gaattcctgc aggatatctg gatccacgaa gcttcccatg
    10621 gtgacgtcac cggttctaga tacctaggtg agctctggta ccctctagtc aaggccttaa
    10681 gtgagtcgta ttacggactg gccgtcgttt tacaggccgg atgggccatg gcaccttaac
    10741 atttctaaat tttagtttaa taaaaaaatt ttaaaataat acgagttttg agactaactt
    10801 tcaataaaaa ccaaaaaatt gcttcaaaaa agagctcgcg ccgatttacg ataaacacag
    10861 cgcgctacga cactccgaaa accgcgccgc accattacat tctccgcccc accgcacggt
    10921 ttgcggaagg cgatgaaatt gctgggccta aaattttaac cctttttttc ccgtttttca
    10981 actgttttcc cgattttttc gatatttttt tcaggttttt agttagaaaa gtatataaaa
    11041 attgcattat ttgtagtttt cgctcgtaat ttgtgataga aaacggccaa tttgcctaga
    11101 tttgtgattc taccaagtgg aatttttggg aaaatatcaa tattccacga tttttattca
    11161 aatcataacg tttcgaaatt atcagcttaa ataaaactaa aataaataat aaaatactaa
    11221 aaaccctgaa tttctcggtt ctcggttcag cttgagttcg ttctatacac gttatcgatt
    11281 tttgtcattt cgtttaatca tgggttttga ttcattttaa atatgaaatt tgatttattc
    11341 ctcgtttcga agttggtttg tcttgtccgg ccggtaaaca aatgttatta aaaaaaataa
    11401 taaatttcga aatttttcgt aatgttccgc aatcgtgttt ttccgtttat ctcgtcttcc
    11461 cttccttcaa atatactatt tattttctca attcacatat tttcagccag ccctgctcaa
    11521 caagtggaat aaaaatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
    11581 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
    11641 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
    11701 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
    11761 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
    11821 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
    11881 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
    11941 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
    12001 atgatctgga cgaagagcat caggggctcg caccagccga actgttcgcc aggctcaagg
    12061 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
    12121 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
    12181 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
    12241 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
    12301 tctatcgcct tcttgacgag ttcttctgag tccaattact cttcaacatc cctacatgct
    12361 ctttctccct gtgctcccac cccctatttt tgttattatc aaaaaacttc tcttaatttc
    12421 tttgtttttt agcttctttt aagccacctc taacaatgaa attgtgtaga ttcaaaaata
    12481 gaattaattc gtaataaaaa gtcgaaaaaa attgtgctcc ctccccccat taataataat
    12541 tctatcccaa aatctacaca atgttctgtg tacacttctt atgtttttta cttctgataa
    12601 atttttttga aacatcatag aaaaaaccgc acacaaaata ccttatcata tgttacgttt
    12661 cagtttatga ccgcaatttt tatttcttcg cacgtctggg cctctcatga cgtcaaatca
    12721 tgctcatcgt gaaaaagttt tggagtattt ttggaatttt tcaatcaagt gaaagtttat
    12781 gaaattaatt ttcctgcttt tgctttttgg ggtttcccct attgtttgtc aagatttcga
    12841 ggacggcgtt tttcttgcta aaatcacaag tattgatgag cacgatgcaa gaaagatcgg
    12901 aagaaggttt gggtttgagg ctcagtggaa ggtgagtaga agttgataat ttgaaagtgg
    12961 agtagtgtct atggggtttt tgccttaaat gacagaatac attcccaata taccaaacat
    13021 aactgtttct cgaggtcgac ggtatcgata agcttgatcc ggatctgcgg cttactcacc
    13081 agacctggcc ccatccgatt accacctatt cgcttcgatg ggacacgcac tcgctgagca
    13141 gcgcttcgat tcttacgaaa gtgtgaaaaa atggctcgat gaatggttcg ccgcaaaaga
    13201 cgatgagttc tactggcgtg gaatccacaa attgcccgag agatgggaaa aatgtgtagc
    13261 tagcgacggc aaatactttg aataaatgat tttttctttt tccacaaaat ttaacgtgtt
    13321 ttttgattta aaaaaaacga catttcatac ttgtacacct gatatctaga tacctaggtg
    13381 agctctggta cccccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
    13441 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
    13501 tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
    13561 accgcatatg gtgcactctc agtgcaatct gctctgatgc cgcatagtta agccagcccc
    13621 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
    13681 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
    13741 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
    13801 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
    13861 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
    13921 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
    13981 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
    14041 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
    14101 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
    14161 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
    14221 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
    14281 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
    14341 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
    14401 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
    14461 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
    14521 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
    14581 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
    14641 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggtcag
    14701 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
    14761 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
    14821 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
    14881 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
    14941 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
    15001 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
    15061 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
    15121 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
    15181 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
    15241 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
    15301 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
    15361 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
    15421 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
    15481 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
    15541 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
    15601 tcctggtctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
    15661 tggataaccg tattaccgcc tttgagtgag ctgatacc
//
