LOCUS       sequence-235341-        1357 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1357)
  TITLE     Davidson HaloTag constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1357)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Halo-Sec61-C-18
FEATURES             Location/Qualifiers
     source          1..1357
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             39..929
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     CDS             993..1280
                     /codon_start=1
                     /gene="SEC61B"
                     /product="human Sec61-beta subunit of the translocon in the
                     endoplasmic reticulum"
                     /label=Sec61-beta
                     /translation="PGPTPSGTNVGSSGRSPSKAVAARAAGSTVRQRKNASCGTRSAGR
                     TTSAGTGGMWRFYTEDSPGLKVGPVPVLVMSLLFIASVFMLHIWGKYTRS"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg tcgccaccat ggcagaaatc ggtactggct
       61 ttccattcga cccccattat gtggaagtcc tgggcgagcg catgcactac gtcgatgttg
      121 gtccgcgcga tggcacccct gtgctgttcc tgcacggtaa cccgacctcc tcctacgtgt
      181 ggcgcaacat catcccgcat gttgcaccga cccatcgctg cattgctcca gacctgatcg
      241 gtatgggcaa atccgacaaa ccagacctgg gttatttctt cgacgaccac gtccgcttca
      301 tggatgcctt catcgaagcc ctgggtctgg aagaggtcgt cctggtcatt cacgactggg
      361 gctccgctct gggtttccac tgggccaagc gcaatccaga gcgcgtcaaa ggtattgcat
      421 ttatggagtt catccgccct atcccgacct gggacgaatg gccagaattt gcccgcgaga
      481 ccttccaggc cttccgcacc accgacgtcg gccgcaagct gatcatcgat cagaacgttt
      541 ttatcgaggg tacgctgccg atgggtgtcg tccgcccgct gactgaagtc gagatggacc
      601 attaccgcga gccgttcctg aatcctgttg accgcgagcc actgtggcgc ttcccaaacg
      661 agctgccaat cgccggtgag ccagcgaaca tcgtcgcgct ggtcgaagaa tacatggact
      721 ggctgcacca gtcccctgtc ccgaagctgc tgttctgggg caccccaggc gttctgatcc
      781 caccggccga agccgctcgc ctggccaaaa gcctgcctaa ctgcaaggct gtggacatcg
      841 gcccgggtct gaatctgctg caagaagaca acccggacct gatcggcagc gagatcgcgc
      901 gctggctgtc gacgctcgag atttccggca tgtacaagtc cggactcaga tctggctcca
      961 gcgcaggcag cgcatccggc ggaagcggaa gccctggtcc gacccccagt ggcactaacg
     1021 tgggatcctc agggcgctct cccagcaaag cagtggccgc ccgggcggcg ggatccactg
     1081 tccggcagag gaaaaatgcc agctgtggga caaggagtgc aggccgcaca acctcggcag
     1141 gcaccggggg gatgtggcga ttctacacag aagattcacc tgggctcaaa gttggccctg
     1201 ttccagtatt ggttatgagt cttctgttca tcgcttctgt atttatgttg cacatttggg
     1261 gcaagtacac tcgttcgtag gaattctgca gtcgacggta ccgcgggccc gggatccacc
     1321 ggatctagat aactgatcat aatcagccat accacat
//
